Computer aided data acquisition tool for high-throughput phenotyping of plant populations.

abstract：Background:Insect herbivory poses a major threat to maize. Benzoxazinoids are important anti-insect secondary metabolites in maize, whose biosynthetic pathway has been extensively studied. However, yet little is known about how benzoxazinoids are regulated in maize, partly due to lack of mutant resources and recalcitra...

journal_title：Plant methods

authors： Gao L,Shen G,Zhang L,Qi J,Zhang C,Ma C,Li J,Wang L,Malook SU,Wu J

更新日期：2019-11-28 00:00:00

abstract：Background:The stomata of plants mainly regulate gas exchange and water dispersion between the interior and external environments of plants and play a major role in the plants' health. The existing methods of stomata segmentation and measurement are mostly for specialized plants. The purpose of this research is to deve...

journal_title：Plant methods

authors： Li K,Huang J,Song W,Wang J,Lv S,Wang X

更新日期：2019-07-03 00:00:00

abstract：BACKGROUND:Allotetraploid white clover (Trifolium repens L.) is an important forage legume widely cultivated in most temperate regions. Only a small number of microsatellite markers are publicly available and can be utilized in white clover breeding programs. The objectives of this study were to develop an integrated a...

journal_title：Plant methods

authors： Zhang Y,He J,Zhao PX,Bouton JH,Monteros MJ

更新日期：2008-07-16 00:00:00

abstract：BACKGROUND:Establishing transcriptional regulatory networks that include protein-protein and protein-DNA interactions has become a key component to better understanding many fundamental biological processes. Although a variety of techniques are available to expose protein-protein and protein-DNA interactions, unequivoc...

journal_title：Plant methods

authors： Xie Z,Grotewold E

更新日期：2008-10-27 00:00:00

abstract：: Three terrestrial plants are known to perform C4 photosynthesis without the dual-cell system by partitioning two distinct types of chloroplasts in separate cytoplasmic compartments. We report herein a protocol for isolating the dimorphic chloroplasts from Bienertia sinuspersici. Hypo-osmotically lysed protoplasts und...

journal_title：Plant methods

authors： Lung SC,Yanagisawa M,Chuong SD

更新日期：2012-03-06 00:00:00

abstract：BACKGROUND:Establishment and maintenance of mutualistic plant-microbial interactions in the rhizosphere and within plant roots involve several root cell types. The processes of host-microbe recognition and infection require complex signal exchange and activation of downstream responses. These molecular events coordinat...

journal_title：Plant methods

authors： Gavrilovic S,Yan Z,Jurkiewicz AM,Stougaard J,Markmann K

更新日期：2016-01-22 00:00:00

abstract：: Generating and identifying transformants is essential for many studies of gene function. In Arabidopsis thaliana, a revolutionary protocol termed floral dip is now the most widely used transformation method. Although robust, it involves a number of relatively time-consuming and laborious steps, including manipulating...

journal_title：Plant methods

authors： Davis AM,Hall A,Millar AJ,Darrah C,Davis SJ

更新日期：2009-02-27 00:00:00

abstract：Background:Most bacteria are not culturable, but can be identified through molecular methods such as metagenomics studies. Due to specific metabolic requirements and symbiotic relationships, these bacteria cannot survive on typical laboratory media. Many economically and medically important bacteria are unculturable; i...

journal_title：Plant methods

authors： Krystel J,Shi Q,Shaw J,Gupta G,Hall D,Stover E

更新日期：2019-07-31 00:00:00

abstract：Background:Plant parasitic weeds belonging to the genus Striga are a major threat for food production in Sub-Saharan Africa and Southeast Asia. The parasite's life cycle starts with the induction of seed germination by host plant-derived signals, followed by parasite attachment, infection, outgrowth, flowering, reprodu...

journal_title：Plant methods

authors： Masteling R,Voorhoeve L,IJsselmuiden J,Dini-Andreote F,de Boer W,Raaijmakers JM

更新日期：2020-05-01 00:00:00

abstract：Background:Open tissue culture technique could be simplified by using different bacteriostatic agents. There is a great difference in the bacteriostatic effects of different antimicrobial agents on various explants. However, there is no report about the effective bacteriostatic agent for open tissue culture of Acacia a...

journal_title：Plant methods

authors： Huang L,Wang H,Shahid MQ,Zeng B

更新日期：2019-01-25 00:00:00

abstract：Background:Deep rooting is one of the most promising plant traits for improving crop yield under water-limited conditions. Most root phenotyping methods are designed for laboratory-grown plants, typically measuring very young plants not grown in soil and not allowing full development of the root system. Results:This s...

journal_title：Plant methods

authors： Chen S,Svane SF,Thorup-Kristensen K

更新日期：2019-12-09 00:00:00

abstract：BACKGROUND:Nectar reabsorption is a widely known phenomenon, related to the strategy of resource-recovery and also to maintain the nectar homeostasis at the nectary. The method currently performed to demonstrate nectar being reabsorbed involves the use of radioactive tracers applied to the nectary. Although this method...

journal_title：Plant methods

authors： Cardoso-Gustavson P,Robazzi Bignelli Valente Aguiar JM,Ricardo Pansarin E,de Barros F

更新日期：2013-06-19 00:00:00

abstract：BACKGROUND:The tomato (Solanum lycopersicum L.) plant is both an economically important food crop and an ideal dicot model to investigate various physiological phenomena not possible in Arabidopsis thaliana. Due to the great diversity of tomato cultivars used by the research community, it is often difficult to reliably...

journal_title：Plant methods

authors： Carvalho RF,Campos ML,Pino LE,Crestana SL,Zsögön A,Lima JE,Benedito VA,Peres LE

更新日期：2011-06-29 00:00:00

abstract：:Primer design is a critical step in the application of PCR-based technologies in gene expression and genetic diversity analysis. As more plant genomes have been sequenced in recent years, the emphasis of primer design strategy has shifted to genome-wide and high-throughput direction. This paper summarizes recent advan...

journal_title：Plant methods

authors： Yang X,Scheffler BE,Weston LA

更新日期：2006-03-01 00:00:00

abstract：BACKGROUND:Protoplasts isolated from leaves are useful materials in plant research. One application, the transient expression of recombinant genes using Arabidopsis mesophyll protoplasts (TEAMP), is currently commonly used for studies of subcellular protein localization, promoter activity, and in vivo protein-protein i...

journal_title：Plant methods

authors： Wu FH,Shen SC,Lee LY,Lee SH,Chan MT,Lin CS

更新日期：2009-11-24 00:00:00

abstract：Background:In-field measurement of yield and growth rate in pasture species is imprecise and costly, limiting scientific and commercial application. Our study proposed a LiDAR-based mobile platform for non-invasive vegetative biomass and growth rate estimation in perennial ryegrass (Lolium perenne L.). This included de...

journal_title：Plant methods

authors： Ghamkhar K,Irie K,Hagedorn M,Hsiao J,Fourie J,Gebbie S,Hoyos-Villegas V,George R,Stewart A,Inch C,Werner A,Barrett B

更新日期：2019-07-10 00:00:00

abstract：:Plants are good sources of biologically active compounds with antimicrobial activity, including polypeptides. Antimicrobial peptides (AMPs) represent one of the main barriers of plant innate immunity to environmental stress factors and are attracting much research interest. There are some extraction methods for isolat...

journal_title：Plant methods

authors： Barashkova AS,Rogozhin EA

更新日期：2020-10-23 00:00:00

abstract：Background:Chloroplasts are critical organelles that perceive and convey metabolic and stress signals to different cellular components, while remaining the seat of photosynthesis and a metabolic factory. The proteomes of intact leaves, chloroplasts, and suborganellar fractions of plastids have been evaluated in the mod...

journal_title：Plant methods

authors： Bhattacharya O,Ortiz I,Walling LL

更新日期：2020-09-24 00:00:00

abstract：BACKGROUND:Plant metabolites are commonly functionally classified, as defense- or growth-related phytohormones, primary and specialized metabolites, and so forth. Analytical procedures for the quantifications of these metabolites are challenging because the metabolites can vary over several orders of magnitude in conce...

journal_title：Plant methods

authors： Schäfer M,Brütting C,Baldwin IT,Kallenbach M

更新日期：2016-05-26 00:00:00

abstract：BACKGROUND:Mitochondria play critical roles in plant growth, development and stress tolerance. Numerous researchers have carried out studies on the plant mitochondrial genome structure, mitochondrial metabolism and nuclear-cytoplasmic interactions. However, classical plant mitochondria extraction methods are time-consu...

journal_title：Plant methods

authors： Xu Y,Li X,Huang J,Peng L,Luo D,Zhang Q,Dan Z,Xiao H,Yang F,Hu J

更新日期：2020-11-04 00:00:00

abstract：Background:Nowadays, there is an increasing demand for fast and reliable plant biomolecular analyses. Conventional methods for the isolation of nucleic acids are time-consuming and require multiple and often non-automatable steps to remove cellular interferences, with consequence that sample preparation is the major bo...

journal_title：Plant methods

authors： Marengo A,Cagliero C,Sgorbini B,Anderson JL,Emaus MN,Bicchi C,Bertea CM,Rubiolo P

更新日期：2019-03-09 00:00:00

abstract：BACKGROUND:RNA interference results in specific gene silencing by small-interfering RNAs (siRNAs). Synthetic siRNAs provide a powerful tool for manipulating gene expression but high cost suggests that novel siRNA production methods are desirable. Strong evolutionary conservation of siRNA structure suggested that siRNAs...

journal_title：Plant methods

authors： Chau BL,Lee KA

更新日期：2007-11-25 00:00:00

abstract：Background:Low pollen viability may limit grapevine yield under certain conditions, causing relevant economic losses to grape-growers. It is usually evaluated by the quantification of the number of viable and non-viable pollen grains that are present in a sample after an adequate pollen grain staining procedure. Althou...

journal_title：Plant methods

authors： Tello J,Montemayor MI,Forneck A,Ibáñez J

更新日期：2018-01-09 00:00:00

abstract：BACKGROUND:Archegoniates (bryophytes, ferns and gymnosperms), such as the moss Physcomitrella patens, possess freely motile sperm cells (spermatozoids) which reach the egg cell via surface water. Although these motile flagellated sperm cells are a traditional botanical subject, they have not been thoroughly analysed in...

journal_title：Plant methods

authors： Horst NA,Reski R

更新日期：2017-05-10 00:00:00

abstract：BACKGROUND:Cyst nematodes are biotrophs that form specialized feeding structures in the roots of host plants, which consist of a syncytial fusion of hypertrophied cells. The formation of syncytium is accompanied by profound transcriptional changes and active metabolism in infected tissues. The challenge in gene express...

journal_title：Plant methods

authors： Anjam MS,Ludwig Y,Hochholdinger F,Miyaura C,Inada M,Siddique S,Grundler FM

更新日期：2016-04-26 00:00:00

abstract：BACKGROUND:Pentatricopeptide-repeat proteins (PPRs) characterized by tandem arrays of a degenerate 35-amino-acid repeat (PPR motif) can bind a single strand RNA and regulate organelle gene expression at the post-transcriptional level, including RNA cleavage, splicing, editing and stability etc. PPRs are conserved in al...

journal_title：Plant methods

authors： Chen J,Zhu H,Huang J,Huang W

更新日期：2020-11-18 00:00:00

abstract：BACKGROUND:The elucidation of complex biological systems requires integration of multiple molecular parameters. Accordingly, high throughput methods like transcriptomics, proteomics, metabolomics and lipidomics have emerged to provide the tools for successful system-wide investigations. Unfortunately, optimized analysi...

journal_title：Plant methods

authors： Salem MA,Jüppner J,Bajdzienko K,Giavalisco P

更新日期：2016-11-10 00:00:00

abstract：UNLABELLED: BACKGROUND:Phytohormones are the key metabolites participating in the regulation of multiple functions of plant organism. Among them, jasmonates, as well as abscisic and salicylic acids are responsible for triggering and modulating plant reactions targeted against pathogens and herbivores, as well as resis...

journal_title：Plant methods

authors： Balcke GU,Handrick V,Bergau N,Fichtner M,Henning A,Stellmach H,Tissier A,Hause B,Frolov A

更新日期：2012-11-22 00:00:00

abstract：:The genomic sequences of many important Triticeae crop species are hard to assemble and analyse due to their large genome sizes, (in part) polyploid genomes and high repeat content. Recently, the draft genomes of barley and bread wheat were reported thanks to cost-efficient and fast NGS technologies. The genome of bar...

journal_title：Plant methods

authors： Spannagl M,Martis MM,Pfeifer M,Nussbaumer T,Mayer KF

更新日期：2013-09-06 00:00:00

abstract：Background:Functional genomic studies using genetics approaches of conifers are hampered by the complex and enormous genome, long vegetative growth period, and exertion in genetic transformation. Thus, the research carried out on gene function in Pinus tabuliformis is typically performed by heterologous expression base...

journal_title：Plant methods

authors： Liu S,Ma J,Liu H,Guo Y,Li W,Niu S

更新日期：2020-04-10 00:00:00