Isolation of antimicrobial peptides from different plant sources: Does a general extraction method exist?

Abstract:

:Plants are good sources of biologically active compounds with antimicrobial activity, including polypeptides. Antimicrobial peptides (AMPs) represent one of the main barriers of plant innate immunity to environmental stress factors and are attracting much research interest. There are some extraction methods for isolation of AMPs from plant organs based on the type of extractant and initial fractionation stages. But most methods are directed to obtain some specific structural types of AMPs and do not allow to understand the molecular diversity of AMP inside a whole plant. In this mini-review, we suggest an optimized scheme of AMP isolation from plants followed by obtaining a set of peptides belonging to various structural families. This approach can be performed for large-scale screening of plants to identify some novel or homologous AMPs for fundamental and applied studies.

journal_name

Plant Methods

journal_title

Plant methods

authors

Barashkova AS,Rogozhin EA

doi

10.1186/s13007-020-00687-1

subject

Has Abstract

pub_date

2020-10-23 00:00:00

pages

143

issn

1746-4811

pii

687

journal_volume

16

pub_type

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