Abstract：
:Primer design is a critical step in the application of PCR-based technologies in gene expression and genetic diversity analysis. As more plant genomes have been sequenced in recent years, the emphasis of primer design strategy has shifted to genome-wide and high-throughput direction. This paper summarizes recent advances in primer design for profiling of DNA polymorphism and mRNA in higher plants, as well as new primer systems developed for animals that can be adapted for plants.
journal_name
Plant Methodsjournal_title
Plant methodsauthors
Yang X,Scheffler BE,Weston LAdoi
10.1186/1746-4811-2-4keywords:
subject
Has Abstractpub_date
2006-03-01 00:00:00pages
4issue
1issn
1746-4811pii
1746-4811-2-4journal_volume
2pub_type
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