Abstract:
:[This corrects the article DOI: 10.1186/s13007-017-0166-6.].
journal_name
Plant Methodsjournal_title
Plant methodsauthors
Lüpke M,Steinbrecher R,Leuchner M,Menzel Adoi
10.1186/s13007-017-0249-4subject
Has Abstractpub_date
2017-11-16 00:00:00pages
100issn
1746-4811pii
249journal_volume
13pub_type
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Plant Methods文献大全abstract:BACKGROUND:In the contexts of genomics, post-genomics and systems biology approaches, data integration presents a major concern. Databases provide crucial solutions: they store, organize and allow information to be queried, they enhance the visibility of newly produced data by comparing them with previously published r...
journal_title:Plant methods
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更新日期:2011-03-29 00:00:00
abstract:BACKGROUND:There is considerable interest in rapid assays or screening systems for assigning gene function. However, analysis of gene function in the flowers of some species is restricted due to the difficulty of producing stably transformed transgenic plants. As a result, experimental approaches based on transient gen...
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abstract:Background:Antirrhinum (snapdragon) species are models for genetic and evolutionary research but recalcitrant to genetic transformation, limiting use of transgenic methods for functional genomics. Transient gene expression from viral vectors and virus-induced gene silencing (VIGS) offer transformation-free alternatives...
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abstract:: Large-scale genetic screens in Arabidopsis are a powerful approach for molecular dissection of complex signaling networks. However, map-based cloning can be time-consuming or even hampered due to low chromosomal recombination. Current strategies using next generation sequencing for molecular identification of mutatio...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/1746-4811-8-12
更新日期:2012-03-30 00:00:00
abstract:BACKGROUND:When conducting plant research, the measurement of photosynthetic pigments can provide basic information on the physiological status of a plant. High-pressure liquid chromatography (HPLC) is becoming widely used for this purpose because it provides an accurate determination of a variety of photosynthetic pig...
journal_title:Plant methods
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doi:10.1186/1746-4811-9-19
更新日期:2013-06-19 00:00:00
abstract:BACKGROUND:Maize haploid breeding technology can be used to rapidly develop homozygous lines, significantly shorten the breeding cycle and improve breeding efficiency. Rapid and accurate sorting haploid kernels is a prerequisite for the large-scale application of this technology. At present, the automatic haploid sorti...
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doi:10.1186/s13007-020-00703-4
更新日期:2021-01-06 00:00:00
abstract:Background:The cellular morphology of plant organs is strongly related to other physical properties such as shape, size, growth, mechanical properties or chemical composition. Cell morphology often vary depending on the type of tissue, or on the distance to a specific tissue. A common challenge in quantitative plant hi...
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doi:10.1186/s13007-020-00603-7
更新日期:2020-05-06 00:00:00
abstract:Background:Plant roots release a variety of organic compounds into the soil which alter the physical, chemical and biological properties of the rhizosphere. Root exudates are technically challenging to measure in soil because roots are difficult to access and exudates can be bound by minerals or consumed by microorgani...
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doi:10.1186/s13007-018-0380-x
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abstract:BACKGROUND:Establishing transcriptional regulatory networks that include protein-protein and protein-DNA interactions has become a key component to better understanding many fundamental biological processes. Although a variety of techniques are available to expose protein-protein and protein-DNA interactions, unequivoc...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/1746-4811-4-25
更新日期:2008-10-27 00:00:00
abstract::Intact protein complexes can be separated by apparent molecular mass using a standard polyacrylamide gel electrophoresis system combining mild detergents and the dye Coomassie Blue. Referring to the blue coloured gel and the gentle method of solubilization yielding native and enzymatically active protein complexes, th...
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doi:10.1186/1746-4811-1-11
更新日期:2005-11-16 00:00:00
abstract:Background:Herbaria are valuable sources of extensive curated plant material that are now accessible to genetic studies because of advances in high-throughput, next-generation sequencing methods. As an applied assessment of large-scale recovery of plastid and ribosomal genome sequences from herbarium material for plant...
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更新日期:2020-01-04 00:00:00
abstract:Background:Lignocellulosic biomass is a complex network of polymers making the cell walls of plants. It represents a feedstock of sustainable resources to be converted into fuels, chemicals and materials. Because of its complex architecture, lignocellulose is a recalcitrant material that necessitates some pretreatments...
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doi:10.1186/s13007-018-0342-3
更新日期:2018-08-27 00:00:00
abstract::Over the last 10 years, high-density SNP arrays and DNA re-sequencing have illuminated the majority of the genotypic space for a number of organisms, including humans, maize, rice and Arabidopsis. For any researcher willing to define and score a phenotype across many individuals, Genome Wide Association Studies (GWAS)...
journal_title:Plant methods
pub_type: 杂志文章,评审
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更新日期:2013-07-22 00:00:00
abstract:Background:Over the last years, several plant science labs have started to employ fluctuating growth light conditions to simulate natural light regimes more closely. Many plant mutants reveal quantifiable effects under fluctuating light despite being indistinguishable from wild-type plants under standard constant light...
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doi:10.1186/s13007-019-0546-1
更新日期:2019-12-23 00:00:00
abstract:BACKGROUND:It is well known that preparation of biological (plant and animal) tissues for Scanning Electron Microscopy (SEM) by chemical fixation and critical point drying results in shrinkage of tissues, often by up to 20-30%, depending on the tissue type and fixation protocol used. We sought to identify a protocol th...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/1746-4811-9-36
更新日期:2013-10-02 00:00:00
abstract:UNLABELLED: BACKGROUND:Phytohormones are the key metabolites participating in the regulation of multiple functions of plant organism. Among them, jasmonates, as well as abscisic and salicylic acids are responsible for triggering and modulating plant reactions targeted against pathogens and herbivores, as well as resis...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/1746-4811-8-47
更新日期:2012-11-22 00:00:00
abstract:UNLABELLED: BACKGROUND:The powdery mildew disease represents a valuable patho-system to study the interaction between plant hosts and obligate biotrophic fungal pathogens. Numerous discoveries have been made on the basis of the quantitative evaluation of plant-powdery mildew interactions, especially in the context of ...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/1746-4811-8-35
更新日期:2012-08-31 00:00:00
abstract:Background:Image processing techniques have been widely used in the analysis of leaf characteristics. Earlier techniques for processing digital RGB color images of plant leaves had several drawbacks, such as inadequate de-noising, and adopting normal-probability statistical estimation models which have few parameters a...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/s13007-020-0561-2
更新日期:2020-02-26 00:00:00
abstract:: Three terrestrial plants are known to perform C4 photosynthesis without the dual-cell system by partitioning two distinct types of chloroplasts in separate cytoplasmic compartments. We report herein a protocol for isolating the dimorphic chloroplasts from Bienertia sinuspersici. Hypo-osmotically lysed protoplasts und...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/1746-4811-8-8
更新日期:2012-03-06 00:00:00
abstract:BACKGROUND:In silico analyses based on sequence similarities with animal channels have identified a large number of plant genes likely to encode ion channels. The attempts made to characterise such putative plant channels at the functional level have most often relied on electrophysiological analyses in classical expre...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/1746-4811-1-14
更新日期:2005-12-19 00:00:00
abstract:Background:Most bacteria are not culturable, but can be identified through molecular methods such as metagenomics studies. Due to specific metabolic requirements and symbiotic relationships, these bacteria cannot survive on typical laboratory media. Many economically and medically important bacteria are unculturable; i...
journal_title:Plant methods
pub_type: 杂志文章
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abstract:BACKGROUND:The main aim of this study was to improve fungal resistance in bread wheat via transgenesis. Transgenic wheat plants harboring barley chitinase (chi26) gene, driven by maize ubi promoter, were obtained using biolistic bombardment, whereas the herbicide resistance gene, bar, driven by the CaMV 35S promoter wa...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/s13007-017-0191-5
更新日期:2017-05-22 00:00:00
abstract:BACKGROUND:Cyst nematodes are biotrophs that form specialized feeding structures in the roots of host plants, which consist of a syncytial fusion of hypertrophied cells. The formation of syncytium is accompanied by profound transcriptional changes and active metabolism in infected tissues. The challenge in gene express...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/s13007-016-0123-9
更新日期:2016-04-26 00:00:00
abstract:BACKGROUND:Fluorescent proteins are extraordinary tools for biology studies due to their versatility; they are used extensively to improve comprehension of plant-microbe interactions. The viral infection process can easily be tracked and imaged in a plant with fluorescent protein-tagged viruses. In plants, fluorescent ...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/1746-4811-10-22
更新日期:2014-07-05 00:00:00
abstract:BACKGROUND:Identifying cis-regulatory elements is critical in understanding the direct and indirect regulatory mechanisms of gene expression. Current approaches include DNase-seq, a technique that combines sensitivity to the nonspecific endonuclease DNase I with high throughput sequencing to identify regions of regulat...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/s13007-015-0087-1
更新日期:2015-09-04 00:00:00
abstract:Background:The demand for effective use of water resources has increased because of ongoing global climate transformations in the agriculture science sector. Cost-effective and timely distributions of the appropriate amount of water are vital not only to maintain a healthy status of plants leaves but to drive the produ...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/s13007-019-0522-9
更新日期:2019-11-18 00:00:00
abstract:Background:Plastome (plastid genome) sequences provide valuable information for understanding the phylogenetic relationships and evolutionary history of plants. Although the rapid development of high-throughput sequencing technology has led to an explosion of plastome sequences, annotation remains a significant bottlen...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/s13007-019-0435-7
更新日期:2019-05-21 00:00:00
abstract:Background:Non-contact resonant ultrasound spectroscopy (NC-RUS) has been proven as a reliable technique for the dynamic determination of leaf water status. It has been already tested in more than 50 plant species. In parallel, relative water content (RWC) is highly used in the ecophysiological field to describe the de...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/s13007-019-0511-z
更新日期:2019-11-07 00:00:00
abstract:Background:High throughput non-destructive phenotyping is emerging as a significant approach for phenotyping germplasm and breeding populations for the identification of superior donors, elite lines, and QTLs. Detection and counting of spikes, the grain bearing organs of wheat, is critical for phenomics of a large set ...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/s13007-020-00582-9
更新日期:2020-03-18 00:00:00
abstract:: Generating and identifying transformants is essential for many studies of gene function. In Arabidopsis thaliana, a revolutionary protocol termed floral dip is now the most widely used transformation method. Although robust, it involves a number of relatively time-consuming and laborious steps, including manipulating...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/1746-4811-5-3
更新日期:2009-02-27 00:00:00