Abstract：
:Intact protein complexes can be separated by apparent molecular mass using a standard polyacrylamide gel electrophoresis system combining mild detergents and the dye Coomassie Blue. Referring to the blue coloured gel and the gentle method of solubilization yielding native and enzymatically active protein complexes, this technique has been named Blue-Native Polyacrylamide Gel-Electrophoresis (BN-PAGE). BN-PAGE has become the method of choice for the investigation of the respiratory protein complexes of the electron transfer chains of a range of organisms, including bacteria, yeasts, animals and plants. It allows the separation in two dimensions of extremely hydrophobic protein sets for analysis and also provides information on their native interactions. In this review we discuss the capabilities of BN-PAGE in proteomics and the wider investigation of protein:protein interactions with a focus on its use and potential in plant science.
journal_name
Plant Methodsjournal_title
Plant methodsauthors
Eubel H,Braun HP,Millar AHdoi
10.1186/1746-4811-1-11keywords:
subject
Has Abstractpub_date
2005-11-16 00:00:00pages
11issue
1issn
1746-4811pii
1746-4811-1-11journal_volume
1pub_type
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