A novel rind puncture technique to measure rind thickness and diameter in plant stalks.

abstract：Background:Wheat is one of the most widely grown crop in temperate climates for food and animal feed. In order to meet the demands of the predicted population increase in an ever-changing climate, wheat production needs to dramatically increase. Spike and grain traits are critical determinants of final yield and grain ...

journal_title：Plant methods

authors： Hughes N,Askew K,Scotson CP,Williams K,Sauze C,Corke F,Doonan JH,Nibau C

更新日期：2017-11-01 00:00:00

abstract：BACKGROUND:Identifying cis-regulatory elements is critical in understanding the direct and indirect regulatory mechanisms of gene expression. Current approaches include DNase-seq, a technique that combines sensitivity to the nonspecific endonuclease DNase I with high throughput sequencing to identify regions of regulat...

journal_title：Plant methods

authors： Cumbie JS,Filichkin SA,Megraw M

更新日期：2015-09-04 00:00:00

abstract：BACKGROUND:Striga species are noxious root hemi-parasitic weeds that debilitate cereal production in sub-Saharan Africa (SSA). Control options for Striga are limited and developing Striga resistant crop germplasm is regarded as the best and most sustainable control measure. Efforts to improve germplasm for Striga resis...

journal_title：Plant methods

authors： Runo S,Macharia S,Alakonya A,Machuka J,Sinha N,Scholes J

更新日期：2012-06-21 00:00:00

abstract：:Primer design is a critical step in the application of PCR-based technologies in gene expression and genetic diversity analysis. As more plant genomes have been sequenced in recent years, the emphasis of primer design strategy has shifted to genome-wide and high-throughput direction. This paper summarizes recent advan...

journal_title：Plant methods

authors： Yang X,Scheffler BE,Weston LA

更新日期：2006-03-01 00:00:00

abstract：Background:Insect herbivory poses a major threat to maize. Benzoxazinoids are important anti-insect secondary metabolites in maize, whose biosynthetic pathway has been extensively studied. However, yet little is known about how benzoxazinoids are regulated in maize, partly due to lack of mutant resources and recalcitra...

journal_title：Plant methods

authors： Gao L,Shen G,Zhang L,Qi J,Zhang C,Ma C,Li J,Wang L,Malook SU,Wu J

更新日期：2019-11-28 00:00:00

abstract：Background:Over the last years, several plant science labs have started to employ fluctuating growth light conditions to simulate natural light regimes more closely. Many plant mutants reveal quantifiable effects under fluctuating light despite being indistinguishable from wild-type plants under standard constant light...

journal_title：Plant methods

authors： Schneider D,Lopez LS,Li M,Crawford JD,Kirchhoff H,Kunz HH

更新日期：2019-12-23 00:00:00

abstract：BACKGROUND:Archegoniates (bryophytes, ferns and gymnosperms), such as the moss Physcomitrella patens, possess freely motile sperm cells (spermatozoids) which reach the egg cell via surface water. Although these motile flagellated sperm cells are a traditional botanical subject, they have not been thoroughly analysed in...

journal_title：Plant methods

authors： Horst NA,Reski R

更新日期：2017-05-10 00:00:00

abstract：BACKGROUND:Starch is the most important source of calories for human nutrition and the majority of it is produced by cereal farming. Starch is also used as a renewable raw material in a range of industrial sectors. It can be chemically modified to introduce new physicochemical properties. In this way starch is adapted ...

journal_title：Plant methods

authors： Carciofi M,Blennow A,Nielsen MM,Holm PB,Hebelstrup KH

更新日期：2012-09-07 00:00:00

abstract：BACKGROUND:It is well known that preparation of biological (plant and animal) tissues for Scanning Electron Microscopy (SEM) by chemical fixation and critical point drying results in shrinkage of tissues, often by up to 20-30%, depending on the tissue type and fixation protocol used. We sought to identify a protocol th...

journal_title：Plant methods

authors： Talbot MJ,White RG

更新日期：2013-10-02 00:00:00

abstract：BACKGROUND:Genetic studies on the molecular mechanisms of the regulation of root growth require the characterisation of a specific root phenotype to be linked with a certain genotype. Such studies using classical labour-intensive methods are severely hindered due to the technical limitations that are associated with th...

journal_title：Plant methods

authors： Slota M,Maluszynski M,Szarejko I

更新日期：2016-06-24 00:00:00

abstract：Background:Root is the principal part of plants to absorb water and nutrient, anchor the plant and affect yield and quality. Non-destructive detection of root traits is difficult to perform due to the hidden nature of the root. Therefore, improved methods to measure roots are necessary to support plant breeding, and op...

journal_title：Plant methods

authors： Lu W,Wang X,Wang F

更新日期：2019-03-23 00:00:00

abstract：Background:The demand for effective use of water resources has increased because of ongoing global climate transformations in the agriculture science sector. Cost-effective and timely distributions of the appropriate amount of water are vital not only to maintain a healthy status of plants leaves but to drive the produ...

journal_title：Plant methods

authors： Zahid A,Abbas HT,Ren A,Zoha A,Heidari H,Shah SA,Imran MA,Alomainy A,Abbasi QH

更新日期：2019-11-18 00:00:00

abstract：Background:Non-contact resonant ultrasound spectroscopy (NC-RUS) has been proven as a reliable technique for the dynamic determination of leaf water status. It has been already tested in more than 50 plant species. In parallel, relative water content (RWC) is highly used in the ecophysiological field to describe the de...

journal_title：Plant methods

authors： Fariñas MD,Jimenez-Carretero D,Sancho-Knapik D,Peguero-Pina JJ,Gil-Pelegrín E,Gómez Álvarez-Arenas T

更新日期：2019-11-07 00:00:00

abstract：Background:Strigolactones represent the most recently described group of plant hormones involved in many aspects of plant growth regulation. Simultaneously, root exuded strigolactones mediate rhizosphere signaling towards beneficial arbuscular mycorrhizal fungi, but also attract parasitic plants. The seed germination o...

journal_title：Plant methods

authors： Floková K,Shimels M,Andreo Jimenez B,Bardaro N,Strnad M,Novák O,Bouwmeester HJ

更新日期：2020-09-17 00:00:00

abstract：Background:Functional genomic studies using genetics approaches of conifers are hampered by the complex and enormous genome, long vegetative growth period, and exertion in genetic transformation. Thus, the research carried out on gene function in Pinus tabuliformis is typically performed by heterologous expression base...

journal_title：Plant methods

authors： Liu S,Ma J,Liu H,Guo Y,Li W,Niu S

更新日期：2020-04-10 00:00:00

abstract：BACKGROUND:Plant breeding and genetics demand fast, exact and reproducible phenotyping. Efficient statistical evaluation of phenotyping data requires standardised data storage ensuring long-term data availability while maintaining intellectual property rights. This is state of the art at phenomics centres, which, howev...

journal_title：Plant methods

authors： Köhl K,Gremmels J

更新日期：2015-04-07 00:00:00

abstract：BACKGROUND:Despite increasing demand, imaging the internal structure of plant organs or tissues without the use of transgenic lines expressing fluorescent proteins remains a challenge. Techniques such as magnetic resonance imaging, optical projection tomography or X-ray absorption tomography have been used with various...

journal_title：Plant methods

authors： Rousseau D,Widiez T,Di Tommaso S,Rositi H,Adrien J,Maire E,Langer M,Olivier C,Peyrin F,Rogowsky P

更新日期：2015-12-18 00:00:00

abstract：:The genomic sequences of many important Triticeae crop species are hard to assemble and analyse due to their large genome sizes, (in part) polyploid genomes and high repeat content. Recently, the draft genomes of barley and bread wheat were reported thanks to cost-efficient and fast NGS technologies. The genome of bar...

journal_title：Plant methods

authors： Spannagl M,Martis MM,Pfeifer M,Nussbaumer T,Mayer KF

更新日期：2013-09-06 00:00:00

abstract：: Generating and identifying transformants is essential for many studies of gene function. In Arabidopsis thaliana, a revolutionary protocol termed floral dip is now the most widely used transformation method. Although robust, it involves a number of relatively time-consuming and laborious steps, including manipulating...

journal_title：Plant methods

authors： Davis AM,Hall A,Millar AJ,Darrah C,Davis SJ

更新日期：2009-02-27 00:00:00

abstract：BACKGROUND:When conducting plant research, the measurement of photosynthetic pigments can provide basic information on the physiological status of a plant. High-pressure liquid chromatography (HPLC) is becoming widely used for this purpose because it provides an accurate determination of a variety of photosynthetic pig...

journal_title：Plant methods

authors： Hu X,Tanaka A,Tanaka R

更新日期：2013-06-19 00:00:00

abstract：BACKGROUND:Protein phosphorylation is accepted as a major regulatory pathway in plants. More than 1000 protein kinases are predicted in the Arabidopsis proteome, however, only a few studies look systematically for in vivo protein phosphorylation sites. Owing to the low stoichiometry and low abundance of phosphorylated ...

journal_title：Plant methods

authors： Wolschin F,Weckwerth W

更新日期：2005-11-01 00:00:00

abstract：BACKGROUND:Plant metabolites are commonly functionally classified, as defense- or growth-related phytohormones, primary and specialized metabolites, and so forth. Analytical procedures for the quantifications of these metabolites are challenging because the metabolites can vary over several orders of magnitude in conce...

journal_title：Plant methods

authors： Schäfer M,Brütting C,Baldwin IT,Kallenbach M

更新日期：2016-05-26 00:00:00

abstract：BACKGROUND:Protoplasts isolated from leaves are useful materials in plant research. One application, the transient expression of recombinant genes using Arabidopsis mesophyll protoplasts (TEAMP), is currently commonly used for studies of subcellular protein localization, promoter activity, and in vivo protein-protein i...

journal_title：Plant methods

authors： Wu FH,Shen SC,Lee LY,Lee SH,Chan MT,Lin CS

更新日期：2009-11-24 00:00:00

abstract：BACKGROUND:Effects of abiotic and biotic stresses on plant photosynthetic performance lead to fitness and yield decrease. The maximum quantum efficiency of photosystem II (F v/F m) is a parameter of chlorophyll fluorescence (ChlF) classically used to track changes in photosynthetic performance. Despite recent technical...

journal_title：Plant methods

authors： Bresson J,Vasseur F,Dauzat M,Koch G,Granier C,Vile D

更新日期：2015-03-26 00:00:00

abstract：BACKGROUND:A multitude of different imaging systems are already available to image genetically altered RNA species; however, only a few of these techniques are actually suitable to visualize endogenous RNA. One possibility is to use fluorescently-labelled and hybridization-sensitive probes. In order to yield more infor...

journal_title：Plant methods

authors： Göhring J,Fulcher N,Schilcher K,Barta A,Jacak J

更新日期：2014-05-31 00:00:00

abstract：:Over the last 10 years, high-density SNP arrays and DNA re-sequencing have illuminated the majority of the genotypic space for a number of organisms, including humans, maize, rice and Arabidopsis. For any researcher willing to define and score a phenotype across many individuals, Genome Wide Association Studies (GWAS)...

journal_title：Plant methods

authors： Korte A,Farlow A

更新日期：2013-07-22 00:00:00

abstract：BACKGROUND:The inability to genetically transform any fern species has been a major technical barrier to unlocking fern biology. Initial attempts to overcome this limitation were based on transient transformation approaches or achieved very low efficiencies. A highly efficient method of stable transformation was recent...

journal_title：Plant methods

authors： Plackett AR,Rabbinowitsch EH,Langdale JA

更新日期：2015-07-03 00:00:00

abstract：BACKGROUND:In silico analyses based on sequence similarities with animal channels have identified a large number of plant genes likely to encode ion channels. The attempts made to characterise such putative plant channels at the functional level have most often relied on electrophysiological analyses in classical expre...

journal_title：Plant methods

authors： Hosy E,Duby G,Véry AA,Costa A,Sentenac H,Thibaud JB

更新日期：2005-12-19 00:00:00

abstract：BACKGROUND:Artificial chromosomes (ACs) are a promising next-generation vector for genetic engineering. The most common methods for developing AC constructs are to clone and combine centromeric DNA and telomeric DNA fragments into a single large DNA construct. The AC constructs developed from such methods will contain ...

journal_title：Plant methods

authors： Lin L,Koo DH,Zhang W,St Peter J,Jiang J

更新日期：2011-04-15 00:00:00

abstract：BACKGROUND:Maize haploid breeding technology can be used to rapidly develop homozygous lines, significantly shorten the breeding cycle and improve breeding efficiency. Rapid and accurate sorting haploid kernels is a prerequisite for the large-scale application of this technology. At present, the automatic haploid sorti...

journal_title：Plant methods

authors： Qu Y,Liu Z,Zhang Y,Yang J,Li H

更新日期：2021-01-06 00:00:00