Abstract：
BACKGROUND:Effects of abiotic and biotic stresses on plant photosynthetic performance lead to fitness and yield decrease. The maximum quantum efficiency of photosystem II (F v/F m) is a parameter of chlorophyll fluorescence (ChlF) classically used to track changes in photosynthetic performance. Despite recent technical and methodological advances in ChlF imaging, the spatio-temporal heterogeneity of F v/F m still awaits for standardized and accurate quantification. RESULTS:We developed a method to quantify the dynamics of spatial heterogeneity of photosynthetic efficiency through the distribution-based analysis of F v/F m values. The method was applied to Arabidopsis thaliana grown under well-watered and severe water deficit (survival rate of 40%). First, whole-plant F v/F m shifted from unimodal to bimodal distributions during plant development despite a constant mean F v/F m under well-watered conditions. The establishment of a bimodal distribution of F v/F m reflects the occurrence of two types of leaf regions with contrasted photosynthetic efficiency. The distance between the two modes (called S) quantified the whole-plant photosynthetic heterogeneity. The weighted contribution of the most efficient/healthiest leaf regions to whole-plant performance (called W max) quantified the spatial efficiency of a photosynthetically heterogeneous plant. Plant survival to water deficit was associated to high S values, as well as with strong and fast recovery of W max following soil rewatering. Hence, during stress surviving plants had higher, but more efficient photosynthetic heterogeneity compared to perishing plants. Importantly, S allowed the discrimination between surviving and perishing plants four days earlier than the mean F v/F m. A sensitivity analysis from simulated dynamics of F v/F m showed that parameters indicative of plant tolerance and/or stress intensity caused identifiable changes in S and W max. Finally, an independent comparison of six Arabidopsis accessions grown under well-watered conditions indicated that S and W max are related to the genetic variability of growth. CONCLUSIONS:The distribution-based analysis of ChlF provides an efficient tool for quantifying photosynthetic heterogeneity and performance. S and W max are good indicators to estimate plant survival under water stress. Our results suggest that the dynamics of photosynthetic heterogeneity are key components of plant growth and tolerance to stress.
journal_name
Plant Methodsjournal_title
Plant methodsauthors
Bresson J,Vasseur F,Dauzat M,Koch G,Granier C,Vile Ddoi
10.1186/s13007-015-0067-5subject
Has Abstractpub_date
2015-03-26 00:00:00pages
23issn
1746-4811pii
67journal_volume
11pub_type
杂志文章相关文献
Plant Methods文献大全abstract：Background:Root is the principal part of plants to absorb water and nutrient, anchor the plant and affect yield and quality. Non-destructive detection of root traits is difficult to perform due to the hidden nature of the root. Therefore, improved methods to measure roots are necessary to support plant breeding, and op...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/s13007-019-0414-z
更新日期：2019-03-23 00:00:00
abstract：UNLABELLED: BACKGROUND:The powdery mildew disease represents a valuable patho-system to study the interaction between plant hosts and obligate biotrophic fungal pathogens. Numerous discoveries have been made on the basis of the quantitative evaluation of plant-powdery mildew interactions, especially in the context of ...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/1746-4811-8-35
更新日期：2012-08-31 00:00:00
abstract：BACKGROUND:Establishment and maintenance of mutualistic plant-microbial interactions in the rhizosphere and within plant roots involve several root cell types. The processes of host-microbe recognition and infection require complex signal exchange and activation of downstream responses. These molecular events coordinat...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/s13007-016-0105-y
更新日期：2016-01-22 00:00:00
abstract：Background:An effective and expeditious approach to assess plant nitrogen status is urgently needed in rice production and management as the conventional chemical methods are laborious and time-consuming. Results:Fourier transform infrared photoacoustic spectroscopy (FTIR-PAS) was used to record the spectra of rice le...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/s13007-019-0482-0
更新日期：2019-08-19 00:00:00
abstract：:[This corrects the article DOI: 10.1186/s13007-017-0166-6.]. ...
journal_title：Plant methods
pub_type： 杂志文章,已发布勘误
doi：10.1186/s13007-017-0249-4
更新日期：2017-11-16 00:00:00
abstract：Motivation:In 2005, researchers from the French National Research Institute for Agriculture, Food and Environment (Institut national de recherche pour l'agriculture, l'alimentation et l'environnement, INRAE) started a collaboration with the French farmers' seed network Réseau Semences Paysannes (RSP) on bread wheat par...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/s13007-020-00640-2
更新日期：2020-07-23 00:00:00
abstract：Background:Protein data over circadian time scale is scarce for clock transcription factors. Further work in this direction is required for refining quantitative clock models. However, gathering highly resolved dynamics of low-abundance transcription factors has been a major challenge in the field. In this work we prov...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/s13007-019-0454-4
更新日期：2019-07-08 00:00:00
abstract：BACKGROUND:Transcription factors (TFs) coordinate precise gene expression patterns that give rise to distinct phenotypic outputs. The identification of genes and transcriptional networks regulated by a TF often requires stable transformation and expression changes in plant cells. However, the production of stable trans...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/s13007-016-0141-7
更新日期：2016-10-19 00:00:00
abstract：BACKGROUND:Microsatellites are popular molecular markers in many plant species due to their stable and highly polymorphic nature. A number of analysis methods have been described but analyses of these markers are typically performed on cumbersome polyacrylamide gels or more conveniently by capillary electrophoresis on ...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/1746-4811-4-8
更新日期：2008-05-19 00:00:00
abstract：BACKGROUND:Simultaneous analysis of multiple functional-related phytohormones and their metabolites will improve our understanding of interactions among different hormones in the same biologic process. RESULTS:A method was developed for simultaneous quantification of multiple phytohormones, abscisic acid, indole-3-ace...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/1746-4811-8-2
更新日期：2012-01-15 00:00:00
abstract：:Chloroplasts play a great role for sustained wellbeing of life on the planet. They have the power and raw materials that can be used as sophisticated biological factories. They are rich in energy as they have lots of pigment-protein complexes capable of collecting sunlight, in sugar produced by photosynthesis and in m...
journal_title：Plant methods
pub_type： 杂志文章,评审
doi：10.1186/s13007-017-0179-1
更新日期：2017-04-19 00:00:00
abstract：: Generating and identifying transformants is essential for many studies of gene function. In Arabidopsis thaliana, a revolutionary protocol termed floral dip is now the most widely used transformation method. Although robust, it involves a number of relatively time-consuming and laborious steps, including manipulating...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/1746-4811-5-3
更新日期：2009-02-27 00:00:00
abstract：BACKGROUND:The tomato (Solanum lycopersicum L.) plant is both an economically important food crop and an ideal dicot model to investigate various physiological phenomena not possible in Arabidopsis thaliana. Due to the great diversity of tomato cultivars used by the research community, it is often difficult to reliably...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/1746-4811-7-18
更新日期：2011-06-29 00:00:00
abstract：BACKGROUND:It is well known that preparation of biological (plant and animal) tissues for Scanning Electron Microscopy (SEM) by chemical fixation and critical point drying results in shrinkage of tissues, often by up to 20-30%, depending on the tissue type and fixation protocol used. We sought to identify a protocol th...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/1746-4811-9-36
更新日期：2013-10-02 00:00:00
abstract：Background:Plastome (plastid genome) sequences provide valuable information for understanding the phylogenetic relationships and evolutionary history of plants. Although the rapid development of high-throughput sequencing technology has led to an explosion of plastome sequences, annotation remains a significant bottlen...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/s13007-019-0435-7
更新日期：2019-05-21 00:00:00
abstract：BACKGROUND:Cyst nematodes are biotrophs that form specialized feeding structures in the roots of host plants, which consist of a syncytial fusion of hypertrophied cells. The formation of syncytium is accompanied by profound transcriptional changes and active metabolism in infected tissues. The challenge in gene express...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/s13007-016-0123-9
更新日期：2016-04-26 00:00:00
abstract：:Plants are good sources of biologically active compounds with antimicrobial activity, including polypeptides. Antimicrobial peptides (AMPs) represent one of the main barriers of plant innate immunity to environmental stress factors and are attracting much research interest. There are some extraction methods for isolat...
journal_title：Plant methods
pub_type： 杂志文章,评审
doi：10.1186/s13007-020-00687-1
更新日期：2020-10-23 00:00:00
abstract：Background:Non-contact resonant ultrasound spectroscopy (NC-RUS) has been proven as a reliable technique for the dynamic determination of leaf water status. It has been already tested in more than 50 plant species. In parallel, relative water content (RWC) is highly used in the ecophysiological field to describe the de...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/s13007-019-0511-z
更新日期：2019-11-07 00:00:00
abstract：Background:Most bacteria are not culturable, but can be identified through molecular methods such as metagenomics studies. Due to specific metabolic requirements and symbiotic relationships, these bacteria cannot survive on typical laboratory media. Many economically and medically important bacteria are unculturable; i...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/s13007-019-0465-1
更新日期：2019-07-31 00:00:00
abstract：:Terahertz technology is still an evolving research field that attracts scientists with very different backgrounds working on a wide range of subjects. In the past two decades, it has been demonstrated that terahertz technology can provide a non-invasive tool for measuring and monitoring the water content of leaves and...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/s13007-015-0057-7
更新日期：2015-03-06 00:00:00
abstract：BACKGROUND:Protoplasts isolated from leaves are useful materials in plant research. One application, the transient expression of recombinant genes using Arabidopsis mesophyll protoplasts (TEAMP), is currently commonly used for studies of subcellular protein localization, promoter activity, and in vivo protein-protein i...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/1746-4811-5-16
更新日期：2009-11-24 00:00:00
abstract：BACKGROUND:The main aim of this study was to improve fungal resistance in bread wheat via transgenesis. Transgenic wheat plants harboring barley chitinase (chi26) gene, driven by maize ubi promoter, were obtained using biolistic bombardment, whereas the herbicide resistance gene, bar, driven by the CaMV 35S promoter wa...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/s13007-017-0191-5
更新日期：2017-05-22 00:00:00
abstract：BACKGROUND:Mitochondria play critical roles in plant growth, development and stress tolerance. Numerous researchers have carried out studies on the plant mitochondrial genome structure, mitochondrial metabolism and nuclear-cytoplasmic interactions. However, classical plant mitochondria extraction methods are time-consu...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/s13007-020-00690-6
更新日期：2020-11-04 00:00:00
abstract：BACKGROUND:Transient gene expression systems are indispensable tools in molecular biology. Yet, their routine application is limited to few plant species often requiring substantial equipment and facilities. High chloroplast and chlorophyll content may further impede downstream applications of transformed cells from gr...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/1746-4811-8-14
更新日期：2012-05-04 00:00:00
abstract：BACKGROUND:Striga species are noxious root hemi-parasitic weeds that debilitate cereal production in sub-Saharan Africa (SSA). Control options for Striga are limited and developing Striga resistant crop germplasm is regarded as the best and most sustainable control measure. Efforts to improve germplasm for Striga resis...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/1746-4811-8-20
更新日期：2012-06-21 00:00:00
abstract：Background:Herbaria are valuable sources of extensive curated plant material that are now accessible to genetic studies because of advances in high-throughput, next-generation sequencing methods. As an applied assessment of large-scale recovery of plastid and ribosomal genome sequences from herbarium material for plant...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/s13007-019-0534-5
更新日期：2020-01-04 00:00:00
abstract：Background:Deep rooting is one of the most promising plant traits for improving crop yield under water-limited conditions. Most root phenotyping methods are designed for laboratory-grown plants, typically measuring very young plants not grown in soil and not allowing full development of the root system. Results:This s...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/s13007-019-0533-6
更新日期：2019-12-09 00:00:00
abstract：Background:Image processing techniques have been widely used in the analysis of leaf characteristics. Earlier techniques for processing digital RGB color images of plant leaves had several drawbacks, such as inadequate de-noising, and adopting normal-probability statistical estimation models which have few parameters a...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/s13007-020-0561-2
更新日期：2020-02-26 00:00:00
abstract：BACKGROUND:Kinome profiling aims at the parallel analysis of kinase activities in a cell. Novel developed arrays containing consensus substrates for kinases are used to assess those kinase activities. The arrays described in this paper were already used to determine kinase activities in mammalian systems, but since sub...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/1746-4811-3-3
更新日期：2007-02-12 00:00:00
abstract：BACKGROUND:In silico analyses based on sequence similarities with animal channels have identified a large number of plant genes likely to encode ion channels. The attempts made to characterise such putative plant channels at the functional level have most often relied on electrophysiological analyses in classical expre...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/1746-4811-1-14
更新日期：2005-12-19 00:00:00
