Abstract:
Background:Strigolactones represent the most recently described group of plant hormones involved in many aspects of plant growth regulation. Simultaneously, root exuded strigolactones mediate rhizosphere signaling towards beneficial arbuscular mycorrhizal fungi, but also attract parasitic plants. The seed germination of parasitic plants induced by host strigolactones leads to serious agricultural problems worldwide. More insight in these signaling molecules is hampered by their extremely low concentrations in complex soil and plant tissue matrices, as well as their instability. So far, the combination of tailored isolation-that would replace current unspecific, time-consuming and labour-intensive processing of large samples-and a highly sensitive method for the simultaneous profiling of a broad spectrum of strigolactones has not been reported. Results:Depending on the sample matrix, two different strategies for the rapid extraction of the seven structurally similar strigolactones and highly efficient single-step pre-concentration on polymeric RP SPE sorbent were developed and validated. Compared to conventional methods, controlled temperature during the extraction and the addition of an organic modifier (acetonitrile, acetone) to the extraction solvent helped to tailor strigolactone isolation from low initial amounts of root tissue (150 mg fresh weight, FW) and root exudate (20 ml), which improved both strigolactone stability and sample purity. We have designed an efficient UHPLC separation with sensitive MS/MS detection for simultaneous analysis of seven natural strigolactones including their biosynthetic precursors-carlactone and carlactonoic acid. In combination with the optimized UHPLC-MS/MS method, attomolar detection limits were achieved. The new method allowed successful profiling of seven strigolactones in small exudate and root tissue samples of four different agriculturally important plant species-sorghum, rice, pea and tomato. Conclusion:The established method provides efficient strigolactone extraction with aqueous mixtures of less nucleophilic organic solvents from small root tissue and root exudate samples, in combination with rapid single-step pre-concentration. This method improves strigolactone stability and eliminates the co-extraction and signal of matrix-associated contaminants during the final UHPLC-MS/MS analysis with an electrospray interface, which dramatically increases the overall sensitivity of the analysis. We show that the method can be applied to a variety of plant species.
journal_name
Plant Methodsjournal_title
Plant methodsauthors
Floková K,Shimels M,Andreo Jimenez B,Bardaro N,Strnad M,Novák O,Bouwmeester HJdoi
10.1186/s13007-020-00669-3subject
Has Abstractpub_date
2020-09-17 00:00:00pages
125issn
1746-4811pii
669journal_volume
16pub_type
杂志文章相关文献
Plant Methods文献大全abstract:Background:Lignocellulosic biomass is a complex network of polymers making the cell walls of plants. It represents a feedstock of sustainable resources to be converted into fuels, chemicals and materials. Because of its complex architecture, lignocellulose is a recalcitrant material that necessitates some pretreatments...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/s13007-018-0342-3
更新日期:2018-08-27 00:00:00
abstract:: Microarrays are routine tools for transcript profiling, and genomic tiling arrays such as the Arabidopsis AGRONOMICS1 arrays have been found to be highly suitable for such experiments because changes in genome annotation can be easily integrated at the data analysis level. In a transcript profiling experiment, RNA la...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/1746-4811-8-18
更新日期:2012-06-13 00:00:00
abstract:Background:Plant root apex is the major part to direct the root growth and development by responding to various signals/cues from internal and soil environments. To study and understand root system biology particularly at a molecular and cellular level, an Arabidopsis T-DNA insertional enhancer trap line J3411 expressi...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/s13007-019-0393-0
更新日期:2019-01-31 00:00:00
abstract:Background:Insect herbivory poses a major threat to maize. Benzoxazinoids are important anti-insect secondary metabolites in maize, whose biosynthetic pathway has been extensively studied. However, yet little is known about how benzoxazinoids are regulated in maize, partly due to lack of mutant resources and recalcitra...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/s13007-019-0529-2
更新日期:2019-11-28 00:00:00
abstract:BACKGROUND:Effects of abiotic and biotic stresses on plant photosynthetic performance lead to fitness and yield decrease. The maximum quantum efficiency of photosystem II (F v/F m) is a parameter of chlorophyll fluorescence (ChlF) classically used to track changes in photosynthetic performance. Despite recent technical...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/s13007-015-0067-5
更新日期:2015-03-26 00:00:00
abstract:BACKGROUND:Pollen tubes extend rapidly when pollen grains are incubated in defined media. Tube extension requires many critical functions of plant cells including molecular signaling, cytoskeleton remodeling, secretion, and cell wall synthesis. Consequently, pollen tube growth has been established as a way to conduct p...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/1746-4811-10-3
更新日期:2014-01-23 00:00:00
abstract:Background:In-field measurement of yield and growth rate in pasture species is imprecise and costly, limiting scientific and commercial application. Our study proposed a LiDAR-based mobile platform for non-invasive vegetative biomass and growth rate estimation in perennial ryegrass (Lolium perenne L.). This included de...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/s13007-019-0456-2
更新日期:2019-07-10 00:00:00
abstract:: Large-scale genetic screens in Arabidopsis are a powerful approach for molecular dissection of complex signaling networks. However, map-based cloning can be time-consuming or even hampered due to low chromosomal recombination. Current strategies using next generation sequencing for molecular identification of mutatio...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/1746-4811-8-12
更新日期:2012-03-30 00:00:00
abstract:BACKGROUND:Transcription factors (TFs) coordinate precise gene expression patterns that give rise to distinct phenotypic outputs. The identification of genes and transcriptional networks regulated by a TF often requires stable transformation and expression changes in plant cells. However, the production of stable trans...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/s13007-016-0141-7
更新日期:2016-10-19 00:00:00
abstract:Motivation:In 2005, researchers from the French National Research Institute for Agriculture, Food and Environment (Institut national de recherche pour l'agriculture, l'alimentation et l'environnement, INRAE) started a collaboration with the French farmers' seed network Réseau Semences Paysannes (RSP) on bread wheat par...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/s13007-020-00640-2
更新日期:2020-07-23 00:00:00
abstract:BACKGROUND:In the contexts of genomics, post-genomics and systems biology approaches, data integration presents a major concern. Databases provide crucial solutions: they store, organize and allow information to be queried, they enhance the visibility of newly produced data by comparing them with previously published r...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/1746-4811-7-8
更新日期:2011-03-29 00:00:00
abstract::Modern agriculture has created a demand for plant biotechnology products that provide durable resistance to insect pests, tolerance of herbicide applications for weed control, and agronomic traits tailored for specific geographies. These transgenic trait products require a modular and sequential multigene stacking pla...
journal_title:Plant methods
pub_type: 杂志文章,评审
doi:10.1186/s13007-016-0111-0
更新日期:2016-02-01 00:00:00
abstract:BACKGROUND:A multitude of different imaging systems are already available to image genetically altered RNA species; however, only a few of these techniques are actually suitable to visualize endogenous RNA. One possibility is to use fluorescently-labelled and hybridization-sensitive probes. In order to yield more infor...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/1746-4811-10-15
更新日期:2014-05-31 00:00:00
abstract:BACKGROUND:The data generated during a course of a biological experiment/study can be sometimes be massive and its management becomes quite critical for the success of the investigation undertaken. The accumulation and analysis of such large datasets often becomes tedious for biologists and lab technicians. Most of the...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/1746-4811-5-18
更新日期:2009-12-10 00:00:00
abstract:BACKGROUND:Nectar reabsorption is a widely known phenomenon, related to the strategy of resource-recovery and also to maintain the nectar homeostasis at the nectary. The method currently performed to demonstrate nectar being reabsorbed involves the use of radioactive tracers applied to the nectary. Although this method...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/1746-4811-9-20
更新日期:2013-06-19 00:00:00
abstract:BACKGROUND:The tomato (Solanum lycopersicum L.) plant is both an economically important food crop and an ideal dicot model to investigate various physiological phenomena not possible in Arabidopsis thaliana. Due to the great diversity of tomato cultivars used by the research community, it is often difficult to reliably...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/1746-4811-7-18
更新日期:2011-06-29 00:00:00
abstract:BACKGROUND:Plant breeding and genetics demand fast, exact and reproducible phenotyping. Efficient statistical evaluation of phenotyping data requires standardised data storage ensuring long-term data availability while maintaining intellectual property rights. This is state of the art at phenomics centres, which, howev...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/s13007-015-0069-3
更新日期:2015-04-07 00:00:00
abstract:BACKGROUND:When conducting plant research, the measurement of photosynthetic pigments can provide basic information on the physiological status of a plant. High-pressure liquid chromatography (HPLC) is becoming widely used for this purpose because it provides an accurate determination of a variety of photosynthetic pig...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/1746-4811-9-19
更新日期:2013-06-19 00:00:00
abstract::Receptor-like protein kinases (RLKs) are a large and important group of plant proteins involved in numerous aspects of development and stress response. Within this family, homo-oligermization of receptors followed by autophosphorylation of the intracellular protein kinase domain appears to be a widespread mechanism to...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/1746-4811-9-22
更新日期:2013-06-26 00:00:00
abstract::Chloroplasts play a great role for sustained wellbeing of life on the planet. They have the power and raw materials that can be used as sophisticated biological factories. They are rich in energy as they have lots of pigment-protein complexes capable of collecting sunlight, in sugar produced by photosynthesis and in m...
journal_title:Plant methods
pub_type: 杂志文章,评审
doi:10.1186/s13007-017-0179-1
更新日期:2017-04-19 00:00:00
abstract:BACKGROUND:Kinome profiling aims at the parallel analysis of kinase activities in a cell. Novel developed arrays containing consensus substrates for kinases are used to assess those kinase activities. The arrays described in this paper were already used to determine kinase activities in mammalian systems, but since sub...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/1746-4811-3-3
更新日期:2007-02-12 00:00:00
abstract:Background:Chickpea (Cicer arietinum L.), an important legume crop is one of the major source of dietary protein. Developing an efficient and reproducible transformation method is imperative to expedite functional genomics studies in this crop. Here, we present an optimized and detailed procedure for Agrobacterium rhiz...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/s13007-018-0315-6
更新日期:2018-07-06 00:00:00
abstract:Background:Non-contact resonant ultrasound spectroscopy (NC-RUS) has been proven as a reliable technique for the dynamic determination of leaf water status. It has been already tested in more than 50 plant species. In parallel, relative water content (RWC) is highly used in the ecophysiological field to describe the de...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/s13007-019-0511-z
更新日期:2019-11-07 00:00:00
abstract:Background:Monitoring the timing of seedling emergence and early development via high-throughput phenotyping with computer vision is a challenging topic of high interest in plant science. While most studies focus on the measurements of leaf area index or detection of specific events such as emergence, little attention ...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/s13007-020-00647-9
更新日期:2020-07-30 00:00:00
abstract:BACKGROUND:Cyst nematodes are biotrophs that form specialized feeding structures in the roots of host plants, which consist of a syncytial fusion of hypertrophied cells. The formation of syncytium is accompanied by profound transcriptional changes and active metabolism in infected tissues. The challenge in gene express...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/s13007-016-0123-9
更新日期:2016-04-26 00:00:00
abstract:BACKGROUND:In eukaryotic cells, the proteasome maintains homeostasis by selectively degrading regulatory and misfolded proteins, and in doing so contributes to the amino acid pool. Inhibition of the proteasome in yeast and human cells decreases de novo protein synthesis. However, it is not know if proteasome inhibition...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/s13007-016-0120-z
更新日期:2016-03-16 00:00:00
abstract:Background:Root is the principal part of plants to absorb water and nutrient, anchor the plant and affect yield and quality. Non-destructive detection of root traits is difficult to perform due to the hidden nature of the root. Therefore, improved methods to measure roots are necessary to support plant breeding, and op...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/s13007-019-0414-z
更新日期:2019-03-23 00:00:00
abstract:Background:Plasmopara obducens is the biotrophic oomycete responsible for impatiens downy mildew, a destructive disease of Impatiens that causes high crop loss. Currently, there are no available methods for the microscopic detection of P. obducens from leaves of impatiens, which may be contributing to the spread of the...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/s13007-018-0362-z
更新日期:2018-10-24 00:00:00
abstract::[This corrects the article DOI: 10.1186/s13007-019-0478-9.]. ...
journal_title:Plant methods
pub_type: 已发布勘误
doi:10.1186/s13007-019-0495-8
更新日期:2019-10-10 00:00:00
abstract:BACKGROUND:The elucidation of complex biological systems requires integration of multiple molecular parameters. Accordingly, high throughput methods like transcriptomics, proteomics, metabolomics and lipidomics have emerged to provide the tools for successful system-wide investigations. Unfortunately, optimized analysi...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/s13007-016-0146-2
更新日期:2016-11-10 00:00:00