Abstract:
Background:Plasmopara obducens is the biotrophic oomycete responsible for impatiens downy mildew, a destructive disease of Impatiens that causes high crop loss. Currently, there are no available methods for the microscopic detection of P. obducens from leaves of impatiens, which may be contributing to the spread of the disease. Fluorescence in situ hybridization (FISH) is a sensitive and robust method that uses sequence-specific, fluorescence-labeled oligonucleotide probes to detect target organisms from the environment. To study this important pathogen, we developed and standardized a FISH technique for the visualization of P. obducens from Impatiens walleriana tissues using a species-specific 24-mer oligonucleotide probe designed to target a region of the rRNA internal transcribed spacer 2 (ITS2). Results:Since P. obducens cannot be propagated in vitro, we developed a custom E. coli expression vector that transcribes the P. obducens rRNA-ITS target sequence (clone-FISH) for use as a control and to optimize hybridization conditions. The FISH assay could detect P. obducens sporangiophores, sporangia and oospores, and hyphae from naturally infected I. walleriana leaves and stems. Cross-reactivity was not observed from plant tissue, and the assay did not react when applied to E. coli with self-ligated plasmids and non-target oomycete species. Conclusions:This FISH protocol may provide a valuable tool for the study of this disease and could potentially be used to improve early monitoring of P. obducens, substantially reducing the persistence and spread of this destructive plant pathogen.
journal_name
Plant Methodsjournal_title
Plant methodsauthors
Salgado-Salazar C,Bauchan GR,Wallace EC,Crouch JAdoi
10.1186/s13007-018-0362-zsubject
Has Abstractpub_date
2018-10-24 00:00:00pages
92issn
1746-4811pii
362journal_volume
14pub_type
杂志文章相关文献
Plant Methods文献大全abstract:BACKGROUND:A multitude of different imaging systems are already available to image genetically altered RNA species; however, only a few of these techniques are actually suitable to visualize endogenous RNA. One possibility is to use fluorescently-labelled and hybridization-sensitive probes. In order to yield more infor...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/1746-4811-10-15
更新日期:2014-05-31 00:00:00
abstract:Background:Measurements of rind and culm thickness and stem radius/diameter are important to biomechanical, ecological and physiological plant studies. However, many methods of measuring rind thickness and diameter are labor intensive and induce plant fatality. A novel rind puncture methodology for obtaining measuremen...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/s13007-020-00587-4
更新日期:2020-04-01 00:00:00
abstract:Background:Most bacteria are not culturable, but can be identified through molecular methods such as metagenomics studies. Due to specific metabolic requirements and symbiotic relationships, these bacteria cannot survive on typical laboratory media. Many economically and medically important bacteria are unculturable; i...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/s13007-019-0465-1
更新日期:2019-07-31 00:00:00
abstract:Background:Chloroplasts are critical organelles that perceive and convey metabolic and stress signals to different cellular components, while remaining the seat of photosynthesis and a metabolic factory. The proteomes of intact leaves, chloroplasts, and suborganellar fractions of plastids have been evaluated in the mod...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/s13007-020-00667-5
更新日期:2020-09-24 00:00:00
abstract:Background:To investigate potential effects of herbicide phytotoxic on crops, a major challenge is a lack of non-destructive and rapid methods to detect plant growth that could allow characterization of herbicide-resistant plants. In such a case, hyperspectral imaging can quickly obtain the spectrum for each pixel in t...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/s13007-020-00576-7
更新日期:2020-03-05 00:00:00
abstract::[This corrects the article DOI: 10.1186/s13007-017-0166-6.]. ...
journal_title:Plant methods
pub_type: 杂志文章,已发布勘误
doi:10.1186/s13007-017-0249-4
更新日期:2017-11-16 00:00:00
abstract:Background:Lignocellulosic biomass is a complex network of polymers making the cell walls of plants. It represents a feedstock of sustainable resources to be converted into fuels, chemicals and materials. Because of its complex architecture, lignocellulose is a recalcitrant material that necessitates some pretreatments...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/s13007-018-0342-3
更新日期:2018-08-27 00:00:00
abstract::Receptor-like protein kinases (RLKs) are a large and important group of plant proteins involved in numerous aspects of development and stress response. Within this family, homo-oligermization of receptors followed by autophosphorylation of the intracellular protein kinase domain appears to be a widespread mechanism to...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/1746-4811-9-22
更新日期:2013-06-26 00:00:00
abstract:BACKGROUND:Genetic studies on the molecular mechanisms of the regulation of root growth require the characterisation of a specific root phenotype to be linked with a certain genotype. Such studies using classical labour-intensive methods are severely hindered due to the technical limitations that are associated with th...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/s13007-016-0135-5
更新日期:2016-06-24 00:00:00
abstract::Modern agriculture has created a demand for plant biotechnology products that provide durable resistance to insect pests, tolerance of herbicide applications for weed control, and agronomic traits tailored for specific geographies. These transgenic trait products require a modular and sequential multigene stacking pla...
journal_title:Plant methods
pub_type: 杂志文章,评审
doi:10.1186/s13007-016-0111-0
更新日期:2016-02-01 00:00:00
abstract:BACKGROUND:There is considerable interest in rapid assays or screening systems for assigning gene function. However, analysis of gene function in the flowers of some species is restricted due to the difficulty of producing stably transformed transgenic plants. As a result, experimental approaches based on transient gen...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/1746-4811-3-1
更新日期:2007-01-08 00:00:00
abstract:BACKGROUND:Pentatricopeptide-repeat proteins (PPRs) characterized by tandem arrays of a degenerate 35-amino-acid repeat (PPR motif) can bind a single strand RNA and regulate organelle gene expression at the post-transcriptional level, including RNA cleavage, splicing, editing and stability etc. PPRs are conserved in al...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/s13007-020-00696-0
更新日期:2020-11-18 00:00:00
abstract:: Research in plant molecular biology involves DNA purification on a daily basis. Although different commercial kits enable convenient extraction of high-quality DNA from E. coli cells, PCR and agarose gel samples as well as plant tissues, each kit is designed for a particular type of DNA extraction work, and the cost ...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/1746-4811-6-1
更新日期:2010-01-14 00:00:00
abstract:Background:Progress in high-throughput molecular methods accompanied by more complex experimental designs demands novel data visualisation solutions. To specifically answer the question which parts of the specifical biological system are responding in particular perturbation, integrative approach in which experimental ...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/s13007-018-0345-0
更新日期:2018-08-30 00:00:00
abstract:BACKGROUND:Maize haploid breeding technology can be used to rapidly develop homozygous lines, significantly shorten the breeding cycle and improve breeding efficiency. Rapid and accurate sorting haploid kernels is a prerequisite for the large-scale application of this technology. At present, the automatic haploid sorti...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/s13007-020-00703-4
更新日期:2021-01-06 00:00:00
abstract::Primer design is a critical step in the application of PCR-based technologies in gene expression and genetic diversity analysis. As more plant genomes have been sequenced in recent years, the emphasis of primer design strategy has shifted to genome-wide and high-throughput direction. This paper summarizes recent advan...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/1746-4811-2-4
更新日期:2006-03-01 00:00:00
abstract:BACKGROUND:The elucidation of complex biological systems requires integration of multiple molecular parameters. Accordingly, high throughput methods like transcriptomics, proteomics, metabolomics and lipidomics have emerged to provide the tools for successful system-wide investigations. Unfortunately, optimized analysi...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/s13007-016-0146-2
更新日期:2016-11-10 00:00:00
abstract:BACKGROUND:Establishment and maintenance of mutualistic plant-microbial interactions in the rhizosphere and within plant roots involve several root cell types. The processes of host-microbe recognition and infection require complex signal exchange and activation of downstream responses. These molecular events coordinat...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/s13007-016-0105-y
更新日期:2016-01-22 00:00:00
abstract:Background:An effective and expeditious approach to assess plant nitrogen status is urgently needed in rice production and management as the conventional chemical methods are laborious and time-consuming. Results:Fourier transform infrared photoacoustic spectroscopy (FTIR-PAS) was used to record the spectra of rice le...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/s13007-019-0482-0
更新日期:2019-08-19 00:00:00
abstract:Background:Functional genomic studies using genetics approaches of conifers are hampered by the complex and enormous genome, long vegetative growth period, and exertion in genetic transformation. Thus, the research carried out on gene function in Pinus tabuliformis is typically performed by heterologous expression base...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/s13007-020-00594-5
更新日期:2020-04-10 00:00:00
abstract:Background:Strigolactones represent the most recently described group of plant hormones involved in many aspects of plant growth regulation. Simultaneously, root exuded strigolactones mediate rhizosphere signaling towards beneficial arbuscular mycorrhizal fungi, but also attract parasitic plants. The seed germination o...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/s13007-020-00669-3
更新日期:2020-09-17 00:00:00
abstract::Chloroplasts play a great role for sustained wellbeing of life on the planet. They have the power and raw materials that can be used as sophisticated biological factories. They are rich in energy as they have lots of pigment-protein complexes capable of collecting sunlight, in sugar produced by photosynthesis and in m...
journal_title:Plant methods
pub_type: 杂志文章,评审
doi:10.1186/s13007-017-0179-1
更新日期:2017-04-19 00:00:00
abstract:BACKGROUND:When conducting plant research, the measurement of photosynthetic pigments can provide basic information on the physiological status of a plant. High-pressure liquid chromatography (HPLC) is becoming widely used for this purpose because it provides an accurate determination of a variety of photosynthetic pig...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/1746-4811-9-19
更新日期:2013-06-19 00:00:00
abstract:BACKGROUND:Allotetraploid white clover (Trifolium repens L.) is an important forage legume widely cultivated in most temperate regions. Only a small number of microsatellite markers are publicly available and can be utilized in white clover breeding programs. The objectives of this study were to develop an integrated a...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/1746-4811-4-19
更新日期:2008-07-16 00:00:00
abstract:BACKGROUND:Protoplasts isolated from leaves are useful materials in plant research. One application, the transient expression of recombinant genes using Arabidopsis mesophyll protoplasts (TEAMP), is currently commonly used for studies of subcellular protein localization, promoter activity, and in vivo protein-protein i...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/1746-4811-5-16
更新日期:2009-11-24 00:00:00
abstract:BACKGROUND:Transient gene expression systems are indispensable tools in molecular biology. Yet, their routine application is limited to few plant species often requiring substantial equipment and facilities. High chloroplast and chlorophyll content may further impede downstream applications of transformed cells from gr...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/1746-4811-8-14
更新日期:2012-05-04 00:00:00
abstract:BACKGROUND:Identifying cis-regulatory elements is critical in understanding the direct and indirect regulatory mechanisms of gene expression. Current approaches include DNase-seq, a technique that combines sensitivity to the nonspecific endonuclease DNase I with high throughput sequencing to identify regions of regulat...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/s13007-015-0087-1
更新日期:2015-09-04 00:00:00
abstract:Background:Antirrhinum (snapdragon) species are models for genetic and evolutionary research but recalcitrant to genetic transformation, limiting use of transgenic methods for functional genomics. Transient gene expression from viral vectors and virus-induced gene silencing (VIGS) offer transformation-free alternatives...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/s13007-020-00683-5
更新日期:2020-10-27 00:00:00
abstract:BACKGROUND:The main aim of this study was to improve fungal resistance in bread wheat via transgenesis. Transgenic wheat plants harboring barley chitinase (chi26) gene, driven by maize ubi promoter, were obtained using biolistic bombardment, whereas the herbicide resistance gene, bar, driven by the CaMV 35S promoter wa...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/s13007-017-0191-5
更新日期:2017-05-22 00:00:00
abstract:BACKGROUND:Effects of abiotic and biotic stresses on plant photosynthetic performance lead to fitness and yield decrease. The maximum quantum efficiency of photosystem II (F v/F m) is a parameter of chlorophyll fluorescence (ChlF) classically used to track changes in photosynthetic performance. Despite recent technical...
journal_title:Plant methods
pub_type: 杂志文章
doi:10.1186/s13007-015-0067-5
更新日期:2015-03-26 00:00:00