Abstract：
Background:An effective and expeditious approach to assess plant nitrogen status is urgently needed in rice production and management as the conventional chemical methods are laborious and time-consuming. Results:Fourier transform infrared photoacoustic spectroscopy (FTIR-PAS) was used to record the spectra of rice leaves for the effective diagnosis of nitrogen nutrition status. The band in the wavenumber range of 1680 to 1630 cm-1 was associated with amide I and that from 1570 to 1510 cm-1 with amide II. We attempted to use this information to characterize the nitrogen status in rice plants at different growth stages. The ratio of photoacoustic intensity of amide II to amide I was measured and applied as nitrogen status index, and considering the yields, the ratio showed a positive linear correlation (R2 = 0.9) with the total nitrogen of rice leaves. The ratio at the tillering and full panicle stages were more suitable for diagnosis, a ratio of 0.4-0.55 indicated an adequate nitrogen status, ratios lower than 0.4 indicated a poor nitrogen status; whereas ratios greater than 0.55 indicated excessive nitrogen supply. Conclusion:Our study provides an effective and rapid strategy for nitrogen-supply assessment in rice based on FTIR-PAS, which can guide rational fertilization in rice production.
journal_name
Plant Methodsjournal_title
Plant methodsauthors
Wu K,Du C,Ma F,Shen Y,Liang D,Zhou Jdoi
10.1186/s13007-019-0482-0subject
Has Abstractpub_date
2019-08-19 00:00:00pages
94issn
1746-4811pii
482journal_volume
15pub_type
杂志文章相关文献
Plant Methods文献大全abstract：BACKGROUND:Mitochondria play critical roles in plant growth, development and stress tolerance. Numerous researchers have carried out studies on the plant mitochondrial genome structure, mitochondrial metabolism and nuclear-cytoplasmic interactions. However, classical plant mitochondria extraction methods are time-consu...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/s13007-020-00690-6
更新日期：2020-11-04 00:00:00
abstract：Background:Strigolactones represent the most recently described group of plant hormones involved in many aspects of plant growth regulation. Simultaneously, root exuded strigolactones mediate rhizosphere signaling towards beneficial arbuscular mycorrhizal fungi, but also attract parasitic plants. The seed germination o...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/s13007-020-00669-3
更新日期：2020-09-17 00:00:00
abstract：BACKGROUND:The main aim of this study was to improve fungal resistance in bread wheat via transgenesis. Transgenic wheat plants harboring barley chitinase (chi26) gene, driven by maize ubi promoter, were obtained using biolistic bombardment, whereas the herbicide resistance gene, bar, driven by the CaMV 35S promoter wa...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/s13007-017-0191-5
更新日期：2017-05-22 00:00:00
abstract：BACKGROUND:In the contexts of genomics, post-genomics and systems biology approaches, data integration presents a major concern. Databases provide crucial solutions: they store, organize and allow information to be queried, they enhance the visibility of newly produced data by comparing them with previously published r...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/1746-4811-7-8
更新日期：2011-03-29 00:00:00
abstract：BACKGROUND:A multitude of different imaging systems are already available to image genetically altered RNA species; however, only a few of these techniques are actually suitable to visualize endogenous RNA. One possibility is to use fluorescently-labelled and hybridization-sensitive probes. In order to yield more infor...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/1746-4811-10-15
更新日期：2014-05-31 00:00:00
abstract：Background:Charcoal rot is a fungal disease that thrives in warm dry conditions and affects the yield of soybeans and other important agronomic crops worldwide. There is a need for robust, automatic and consistent early detection and quantification of disease symptoms which are important in breeding programs for the de...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/s13007-018-0349-9
更新日期：2018-10-03 00:00:00
abstract：:Chloroplasts play a great role for sustained wellbeing of life on the planet. They have the power and raw materials that can be used as sophisticated biological factories. They are rich in energy as they have lots of pigment-protein complexes capable of collecting sunlight, in sugar produced by photosynthesis and in m...
journal_title：Plant methods
pub_type： 杂志文章,评审
doi：10.1186/s13007-017-0179-1
更新日期：2017-04-19 00:00:00
abstract：Background:Image processing techniques have been widely used in the analysis of leaf characteristics. Earlier techniques for processing digital RGB color images of plant leaves had several drawbacks, such as inadequate de-noising, and adopting normal-probability statistical estimation models which have few parameters a...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/s13007-020-0561-2
更新日期：2020-02-26 00:00:00
abstract：Background:Plant root apex is the major part to direct the root growth and development by responding to various signals/cues from internal and soil environments. To study and understand root system biology particularly at a molecular and cellular level, an Arabidopsis T-DNA insertional enhancer trap line J3411 expressi...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/s13007-019-0393-0
更新日期：2019-01-31 00:00:00
abstract：BACKGROUND:Leaf rust (LR), caused by Puccinia triticina and is an important disease of wheat (Triticum aestivum L.). The most sustainable method for controlling rust diseases is deployment of cultivars incorporating adult plant resistance (APR). However, phenotyping breeding populations or germplasm collections for res...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/s13007-016-0117-7
更新日期：2016-03-02 00:00:00
abstract：BACKGROUND:Protein phosphorylation is accepted as a major regulatory pathway in plants. More than 1000 protein kinases are predicted in the Arabidopsis proteome, however, only a few studies look systematically for in vivo protein phosphorylation sites. Owing to the low stoichiometry and low abundance of phosphorylated ...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/1746-4811-1-9
更新日期：2005-11-01 00:00:00
abstract：BACKGROUND:Simultaneous analysis of multiple functional-related phytohormones and their metabolites will improve our understanding of interactions among different hormones in the same biologic process. RESULTS:A method was developed for simultaneous quantification of multiple phytohormones, abscisic acid, indole-3-ace...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/1746-4811-8-2
更新日期：2012-01-15 00:00:00
abstract：Background:To investigate potential effects of herbicide phytotoxic on crops, a major challenge is a lack of non-destructive and rapid methods to detect plant growth that could allow characterization of herbicide-resistant plants. In such a case, hyperspectral imaging can quickly obtain the spectrum for each pixel in t...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/s13007-020-00576-7
更新日期：2020-03-05 00:00:00
abstract：Background:Monitoring the timing of seedling emergence and early development via high-throughput phenotyping with computer vision is a challenging topic of high interest in plant science. While most studies focus on the measurements of leaf area index or detection of specific events such as emergence, little attention ...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/s13007-020-00647-9
更新日期：2020-07-30 00:00:00
abstract：: Three terrestrial plants are known to perform C4 photosynthesis without the dual-cell system by partitioning two distinct types of chloroplasts in separate cytoplasmic compartments. We report herein a protocol for isolating the dimorphic chloroplasts from Bienertia sinuspersici. Hypo-osmotically lysed protoplasts und...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/1746-4811-8-8
更新日期：2012-03-06 00:00:00
abstract：: We report on a detailed chromatin immunoprecipitation (ChIP) protocol for the unicellular green alga Chlamydomonas reinhardtii. The protocol is suitable for the analysis of nucleosome occupancy, histone modifications and transcription factor binding sites at the level of mononucleosomes for targeted and genome-wide s...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/1746-4811-7-35
更新日期：2011-11-03 00:00:00
abstract：BACKGROUND:Microsatellites are popular molecular markers in many plant species due to their stable and highly polymorphic nature. A number of analysis methods have been described but analyses of these markers are typically performed on cumbersome polyacrylamide gels or more conveniently by capillary electrophoresis on ...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/1746-4811-4-8
更新日期：2008-05-19 00:00:00
abstract：Background:Protein data over circadian time scale is scarce for clock transcription factors. Further work in this direction is required for refining quantitative clock models. However, gathering highly resolved dynamics of low-abundance transcription factors has been a major challenge in the field. In this work we prov...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/s13007-019-0454-4
更新日期：2019-07-08 00:00:00
abstract：Background:Low pollen viability may limit grapevine yield under certain conditions, causing relevant economic losses to grape-growers. It is usually evaluated by the quantification of the number of viable and non-viable pollen grains that are present in a sample after an adequate pollen grain staining procedure. Althou...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/s13007-017-0267-2
更新日期：2018-01-09 00:00:00
abstract：BACKGROUND:In silico analyses based on sequence similarities with animal channels have identified a large number of plant genes likely to encode ion channels. The attempts made to characterise such putative plant channels at the functional level have most often relied on electrophysiological analyses in classical expre...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/1746-4811-1-14
更新日期：2005-12-19 00:00:00
abstract：Background:The demand for effective use of water resources has increased because of ongoing global climate transformations in the agriculture science sector. Cost-effective and timely distributions of the appropriate amount of water are vital not only to maintain a healthy status of plants leaves but to drive the produ...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/s13007-019-0522-9
更新日期：2019-11-18 00:00:00
abstract：BACKGROUND:Cyst nematodes are biotrophs that form specialized feeding structures in the roots of host plants, which consist of a syncytial fusion of hypertrophied cells. The formation of syncytium is accompanied by profound transcriptional changes and active metabolism in infected tissues. The challenge in gene express...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/s13007-016-0123-9
更新日期：2016-04-26 00:00:00
abstract：BACKGROUND:Kinome profiling aims at the parallel analysis of kinase activities in a cell. Novel developed arrays containing consensus substrates for kinases are used to assess those kinase activities. The arrays described in this paper were already used to determine kinase activities in mammalian systems, but since sub...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/1746-4811-3-3
更新日期：2007-02-12 00:00:00
abstract：Motivation:In 2005, researchers from the French National Research Institute for Agriculture, Food and Environment (Institut national de recherche pour l'agriculture, l'alimentation et l'environnement, INRAE) started a collaboration with the French farmers' seed network Réseau Semences Paysannes (RSP) on bread wheat par...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/s13007-020-00640-2
更新日期：2020-07-23 00:00:00
abstract：BACKGROUND:When conducting plant research, the measurement of photosynthetic pigments can provide basic information on the physiological status of a plant. High-pressure liquid chromatography (HPLC) is becoming widely used for this purpose because it provides an accurate determination of a variety of photosynthetic pig...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/1746-4811-9-19
更新日期：2013-06-19 00:00:00
abstract：UNLABELLED: BACKGROUND:The powdery mildew disease represents a valuable patho-system to study the interaction between plant hosts and obligate biotrophic fungal pathogens. Numerous discoveries have been made on the basis of the quantitative evaluation of plant-powdery mildew interactions, especially in the context of ...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/1746-4811-8-35
更新日期：2012-08-31 00:00:00
abstract：BACKGROUND:There is considerable interest in rapid assays or screening systems for assigning gene function. However, analysis of gene function in the flowers of some species is restricted due to the difficulty of producing stably transformed transgenic plants. As a result, experimental approaches based on transient gen...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/1746-4811-3-1
更新日期：2007-01-08 00:00:00
abstract：Background:Over the last years, several plant science labs have started to employ fluctuating growth light conditions to simulate natural light regimes more closely. Many plant mutants reveal quantifiable effects under fluctuating light despite being indistinguishable from wild-type plants under standard constant light...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/s13007-019-0546-1
更新日期：2019-12-23 00:00:00
abstract：: Research in plant molecular biology involves DNA purification on a daily basis. Although different commercial kits enable convenient extraction of high-quality DNA from E. coli cells, PCR and agarose gel samples as well as plant tissues, each kit is designed for a particular type of DNA extraction work, and the cost ...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/1746-4811-6-1
更新日期：2010-01-14 00:00:00
abstract：Background:Deep rooting is one of the most promising plant traits for improving crop yield under water-limited conditions. Most root phenotyping methods are designed for laboratory-grown plants, typically measuring very young plants not grown in soil and not allowing full development of the root system. Results:This s...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/s13007-019-0533-6
更新日期：2019-12-09 00:00:00
