Recent achievements obtained by chloroplast transformation.

abstract：BACKGROUND:Identifying cis-regulatory elements is critical in understanding the direct and indirect regulatory mechanisms of gene expression. Current approaches include DNase-seq, a technique that combines sensitivity to the nonspecific endonuclease DNase I with high throughput sequencing to identify regions of regulat...

journal_title：Plant methods

authors： Cumbie JS,Filichkin SA,Megraw M

更新日期：2015-09-04 00:00:00

abstract：BACKGROUND:It is well known that preparation of biological (plant and animal) tissues for Scanning Electron Microscopy (SEM) by chemical fixation and critical point drying results in shrinkage of tissues, often by up to 20-30%, depending on the tissue type and fixation protocol used. We sought to identify a protocol th...

journal_title：Plant methods

authors： Talbot MJ,White RG

更新日期：2013-10-02 00:00:00

abstract：BACKGROUND:In silico analyses based on sequence similarities with animal channels have identified a large number of plant genes likely to encode ion channels. The attempts made to characterise such putative plant channels at the functional level have most often relied on electrophysiological analyses in classical expre...

journal_title：Plant methods

authors： Hosy E,Duby G,Véry AA,Costa A,Sentenac H,Thibaud JB

更新日期：2005-12-19 00:00:00

abstract：Background:Wheat is one of the most widely grown crop in temperate climates for food and animal feed. In order to meet the demands of the predicted population increase in an ever-changing climate, wheat production needs to dramatically increase. Spike and grain traits are critical determinants of final yield and grain ...

journal_title：Plant methods

authors： Hughes N,Askew K,Scotson CP,Williams K,Sauze C,Corke F,Doonan JH,Nibau C

更新日期：2017-11-01 00:00:00

abstract：Background:Assessing the impact of the environment on plant performance requires growing plants under controlled environmental conditions. Plant phenotypes are a product of genotype × environment (G × E), and the Enviratron at Iowa State University is a facility for testing under controlled conditions the effects of th...

journal_title：Plant methods

authors： Bao Y,Zarecor S,Shah D,Tuel T,Campbell DA,Chapman AVE,Imberti D,Kiekhaefer D,Imberti H,Lübberstedt T,Yin Y,Nettleton D,Lawrence-Dill CJ,Whitham SA,Tang L,Howell SH

更新日期：2019-10-23 00:00:00

abstract：UNLABELLED: BACKGROUND:The powdery mildew disease represents a valuable patho-system to study the interaction between plant hosts and obligate biotrophic fungal pathogens. Numerous discoveries have been made on the basis of the quantitative evaluation of plant-powdery mildew interactions, especially in the context of ...

journal_title：Plant methods

authors： Weßling R,Panstruga R

更新日期：2012-08-31 00:00:00

abstract：BACKGROUND:Transient gene expression systems are indispensable tools in molecular biology. Yet, their routine application is limited to few plant species often requiring substantial equipment and facilities. High chloroplast and chlorophyll content may further impede downstream applications of transformed cells from gr...

journal_title：Plant methods

authors： Pitzschke A,Persak H

更新日期：2012-05-04 00:00:00

abstract：Background:Plant root apex is the major part to direct the root growth and development by responding to various signals/cues from internal and soil environments. To study and understand root system biology particularly at a molecular and cellular level, an Arabidopsis T-DNA insertional enhancer trap line J3411 expressi...

journal_title：Plant methods

authors： Zhang L,Qin LN,Zeng ZR,Wu CZ,Gong YY,Liu LH,Cao FQ

更新日期：2019-01-31 00:00:00

abstract：: Generating and identifying transformants is essential for many studies of gene function. In Arabidopsis thaliana, a revolutionary protocol termed floral dip is now the most widely used transformation method. Although robust, it involves a number of relatively time-consuming and laborious steps, including manipulating...

journal_title：Plant methods

authors： Davis AM,Hall A,Millar AJ,Darrah C,Davis SJ

更新日期：2009-02-27 00:00:00

abstract：BACKGROUND:Despite increasing demand, imaging the internal structure of plant organs or tissues without the use of transgenic lines expressing fluorescent proteins remains a challenge. Techniques such as magnetic resonance imaging, optical projection tomography or X-ray absorption tomography have been used with various...

journal_title：Plant methods

authors： Rousseau D,Widiez T,Di Tommaso S,Rositi H,Adrien J,Maire E,Langer M,Olivier C,Peyrin F,Rogowsky P

更新日期：2015-12-18 00:00:00

abstract：Background:Nowadays, there is an increasing demand for fast and reliable plant biomolecular analyses. Conventional methods for the isolation of nucleic acids are time-consuming and require multiple and often non-automatable steps to remove cellular interferences, with consequence that sample preparation is the major bo...

journal_title：Plant methods

authors： Marengo A,Cagliero C,Sgorbini B,Anderson JL,Emaus MN,Bicchi C,Bertea CM,Rubiolo P

更新日期：2019-03-09 00:00:00

abstract：:Plants are good sources of biologically active compounds with antimicrobial activity, including polypeptides. Antimicrobial peptides (AMPs) represent one of the main barriers of plant innate immunity to environmental stress factors and are attracting much research interest. There are some extraction methods for isolat...

journal_title：Plant methods

authors： Barashkova AS,Rogozhin EA

更新日期：2020-10-23 00:00:00

abstract：Background:Woody tropical plants contain high levels of complex organic compounds that inhibit the chemical procedures needed to extract RNA or DNA, thus compromising downstream applications such as RNA sequencing and analysis of gene expression. To overcome this issue, researchers must use extraction protocols using C...

journal_title：Plant methods

authors： Barbier FF,Chabikwa TG,Ahsan MU,Cook SE,Powell R,Tanurdzic M,Beveridge CA

更新日期：2019-06-05 00:00:00

abstract：BACKGROUND:Effects of abiotic and biotic stresses on plant photosynthetic performance lead to fitness and yield decrease. The maximum quantum efficiency of photosystem II (F v/F m) is a parameter of chlorophyll fluorescence (ChlF) classically used to track changes in photosynthetic performance. Despite recent technical...

journal_title：Plant methods

authors： Bresson J,Vasseur F,Dauzat M,Koch G,Granier C,Vile D

更新日期：2015-03-26 00:00:00

abstract：Background:Open tissue culture technique could be simplified by using different bacteriostatic agents. There is a great difference in the bacteriostatic effects of different antimicrobial agents on various explants. However, there is no report about the effective bacteriostatic agent for open tissue culture of Acacia a...

journal_title：Plant methods

authors： Huang L,Wang H,Shahid MQ,Zeng B

更新日期：2019-01-25 00:00:00

abstract：:Intact protein complexes can be separated by apparent molecular mass using a standard polyacrylamide gel electrophoresis system combining mild detergents and the dye Coomassie Blue. Referring to the blue coloured gel and the gentle method of solubilization yielding native and enzymatically active protein complexes, th...

journal_title：Plant methods

authors： Eubel H,Braun HP,Millar AH

更新日期：2005-11-16 00:00:00

abstract：UNLABELLED: BACKGROUND:Phytohormones are the key metabolites participating in the regulation of multiple functions of plant organism. Among them, jasmonates, as well as abscisic and salicylic acids are responsible for triggering and modulating plant reactions targeted against pathogens and herbivores, as well as resis...

journal_title：Plant methods

authors： Balcke GU,Handrick V,Bergau N,Fichtner M,Henning A,Stellmach H,Tissier A,Hause B,Frolov A

更新日期：2012-11-22 00:00:00

abstract：Background:Herbaria are valuable sources of extensive curated plant material that are now accessible to genetic studies because of advances in high-throughput, next-generation sequencing methods. As an applied assessment of large-scale recovery of plastid and ribosomal genome sequences from herbarium material for plant...

journal_title：Plant methods

authors： Nevill PG,Zhong X,Tonti-Filippini J,Byrne M,Hislop M,Thiele K,van Leeuwen S,Boykin LM,Small I

更新日期：2020-01-04 00:00:00

abstract：:Receptor-like protein kinases (RLKs) are a large and important group of plant proteins involved in numerous aspects of development and stress response. Within this family, homo-oligermization of receptors followed by autophosphorylation of the intracellular protein kinase domain appears to be a widespread mechanism to...

journal_title：Plant methods

authors： Taylor I,Seitz K,Bennewitz S,Walker JC

更新日期：2013-06-26 00:00:00

abstract：Background:Image processing techniques have been widely used in the analysis of leaf characteristics. Earlier techniques for processing digital RGB color images of plant leaves had several drawbacks, such as inadequate de-noising, and adopting normal-probability statistical estimation models which have few parameters a...

journal_title：Plant methods

authors： Chen Z,Wang F,Zhang P,Ke C,Zhu Y,Cao W,Jiang H

更新日期：2020-02-26 00:00:00

abstract：Background:Non-contact resonant ultrasound spectroscopy (NC-RUS) has been proven as a reliable technique for the dynamic determination of leaf water status. It has been already tested in more than 50 plant species. In parallel, relative water content (RWC) is highly used in the ecophysiological field to describe the de...

journal_title：Plant methods

authors： Fariñas MD,Jimenez-Carretero D,Sancho-Knapik D,Peguero-Pina JJ,Gil-Pelegrín E,Gómez Álvarez-Arenas T

更新日期：2019-11-07 00:00:00

abstract：Background:Chickpea (Cicer arietinum L.), an important legume crop is one of the major source of dietary protein. Developing an efficient and reproducible transformation method is imperative to expedite functional genomics studies in this crop. Here, we present an optimized and detailed procedure for Agrobacterium rhiz...

journal_title：Plant methods

authors： Aggarwal PR,Nag P,Choudhary P,Chakraborty N,Chakraborty S

更新日期：2018-07-06 00:00:00

abstract：BACKGROUND:Fluorescent proteins are extraordinary tools for biology studies due to their versatility; they are used extensively to improve comprehension of plant-microbe interactions. The viral infection process can easily be tracked and imaged in a plant with fluorescent protein-tagged viruses. In plants, fluorescent ...

journal_title：Plant methods

authors： Pasin F,Kulasekaran S,Natale P,Simón-Mateo C,García JA

更新日期：2014-07-05 00:00:00

abstract：Background:Over the last years, several plant science labs have started to employ fluctuating growth light conditions to simulate natural light regimes more closely. Many plant mutants reveal quantifiable effects under fluctuating light despite being indistinguishable from wild-type plants under standard constant light...

journal_title：Plant methods

authors： Schneider D,Lopez LS,Li M,Crawford JD,Kirchhoff H,Kunz HH

更新日期：2019-12-23 00:00:00

abstract：Background:Root is the principal part of plants to absorb water and nutrient, anchor the plant and affect yield and quality. Non-destructive detection of root traits is difficult to perform due to the hidden nature of the root. Therefore, improved methods to measure roots are necessary to support plant breeding, and op...

journal_title：Plant methods

authors： Lu W,Wang X,Wang F

更新日期：2019-03-23 00:00:00

abstract：Background:Functional genomic studies using genetics approaches of conifers are hampered by the complex and enormous genome, long vegetative growth period, and exertion in genetic transformation. Thus, the research carried out on gene function in Pinus tabuliformis is typically performed by heterologous expression base...

journal_title：Plant methods

authors： Liu S,Ma J,Liu H,Guo Y,Li W,Niu S

更新日期：2020-04-10 00:00:00

abstract：BACKGROUND:The main aim of this study was to improve fungal resistance in bread wheat via transgenesis. Transgenic wheat plants harboring barley chitinase (chi26) gene, driven by maize ubi promoter, were obtained using biolistic bombardment, whereas the herbicide resistance gene, bar, driven by the CaMV 35S promoter wa...

journal_title：Plant methods

authors： Eissa HF,Hassanien SE,Ramadan AM,El-Shamy MM,Saleh OM,Shokry AM,Abdelsattar M,Morsy YB,El-Maghraby MA,Alameldin HF,Hassan SM,Osman GH,Mahfouz HT,Gad El-Karim GA,Madkour MA,Bahieldin A

更新日期：2017-05-22 00:00:00

abstract：Background:Strigolactones represent the most recently described group of plant hormones involved in many aspects of plant growth regulation. Simultaneously, root exuded strigolactones mediate rhizosphere signaling towards beneficial arbuscular mycorrhizal fungi, but also attract parasitic plants. The seed germination o...

journal_title：Plant methods

authors： Floková K,Shimels M,Andreo Jimenez B,Bardaro N,Strnad M,Novák O,Bouwmeester HJ

更新日期：2020-09-17 00:00:00

abstract：BACKGROUND:Starch is the most important source of calories for human nutrition and the majority of it is produced by cereal farming. Starch is also used as a renewable raw material in a range of industrial sectors. It can be chemically modified to introduce new physicochemical properties. In this way starch is adapted ...

journal_title：Plant methods

authors： Carciofi M,Blennow A,Nielsen MM,Holm PB,Hebelstrup KH

更新日期：2012-09-07 00:00:00

abstract：BACKGROUND:Brassinosteriods (BRs) are a group of important phytohormones that have major effects on plant growth and development. To fully elucidate the function of BRs, a sensitive BR assay is required. However, most of the previously reported methods are tedious and time-consuming due to multiple pretreatment steps. ...

journal_title：Plant methods

authors： Ding J,Wu JH,Liu JF,Yuan BF,Feng YQ

更新日期：2014-11-24 00:00:00