Genotype-independent Agrobacterium rhizogenes-mediated root transformation of chickpea: a rapid and efficient method for reverse genetics studies.

abstract：BACKGROUND:Plant breeding and genetics demand fast, exact and reproducible phenotyping. Efficient statistical evaluation of phenotyping data requires standardised data storage ensuring long-term data availability while maintaining intellectual property rights. This is state of the art at phenomics centres, which, howev...

journal_title：Plant methods

authors： Köhl K,Gremmels J

更新日期：2015-04-07 00:00:00

abstract：BACKGROUND:Archegoniates (bryophytes, ferns and gymnosperms), such as the moss Physcomitrella patens, possess freely motile sperm cells (spermatozoids) which reach the egg cell via surface water. Although these motile flagellated sperm cells are a traditional botanical subject, they have not been thoroughly analysed in...

journal_title：Plant methods

authors： Horst NA,Reski R

更新日期：2017-05-10 00:00:00

abstract：Background:Plant root apex is the major part to direct the root growth and development by responding to various signals/cues from internal and soil environments. To study and understand root system biology particularly at a molecular and cellular level, an Arabidopsis T-DNA insertional enhancer trap line J3411 expressi...

journal_title：Plant methods

authors： Zhang L,Qin LN,Zeng ZR,Wu CZ,Gong YY,Liu LH,Cao FQ

更新日期：2019-01-31 00:00:00

abstract：BACKGROUND:In silico analyses based on sequence similarities with animal channels have identified a large number of plant genes likely to encode ion channels. The attempts made to characterise such putative plant channels at the functional level have most often relied on electrophysiological analyses in classical expre...

journal_title：Plant methods

authors： Hosy E,Duby G,Véry AA,Costa A,Sentenac H,Thibaud JB

更新日期：2005-12-19 00:00:00

abstract：Background:Assessing the impact of the environment on plant performance requires growing plants under controlled environmental conditions. Plant phenotypes are a product of genotype × environment (G × E), and the Enviratron at Iowa State University is a facility for testing under controlled conditions the effects of th...

journal_title：Plant methods

authors： Bao Y,Zarecor S,Shah D,Tuel T,Campbell DA,Chapman AVE,Imberti D,Kiekhaefer D,Imberti H,Lübberstedt T,Yin Y,Nettleton D,Lawrence-Dill CJ,Whitham SA,Tang L,Howell SH

更新日期：2019-10-23 00:00:00

abstract：BACKGROUND:Simultaneous analysis of multiple functional-related phytohormones and their metabolites will improve our understanding of interactions among different hormones in the same biologic process. RESULTS:A method was developed for simultaneous quantification of multiple phytohormones, abscisic acid, indole-3-ace...

journal_title：Plant methods

authors： Liu H,Li X,Xiao J,Wang S

更新日期：2012-01-15 00:00:00

abstract：BACKGROUND:RNA interference results in specific gene silencing by small-interfering RNAs (siRNAs). Synthetic siRNAs provide a powerful tool for manipulating gene expression but high cost suggests that novel siRNA production methods are desirable. Strong evolutionary conservation of siRNA structure suggested that siRNAs...

journal_title：Plant methods

authors： Chau BL,Lee KA

更新日期：2007-11-25 00:00:00

abstract：BACKGROUND:Leaf rust (LR), caused by Puccinia triticina and is an important disease of wheat (Triticum aestivum L.). The most sustainable method for controlling rust diseases is deployment of cultivars incorporating adult plant resistance (APR). However, phenotyping breeding populations or germplasm collections for res...

journal_title：Plant methods

authors： Riaz A,Periyannan S,Aitken E,Hickey L

更新日期：2016-03-02 00:00:00

abstract：:[This corrects the article DOI: 10.1186/s13007-017-0166-6.]. ...

journal_title：Plant methods

authors： Lüpke M,Steinbrecher R,Leuchner M,Menzel A

更新日期：2017-11-16 00:00:00

abstract：Background:Deep rooting is one of the most promising plant traits for improving crop yield under water-limited conditions. Most root phenotyping methods are designed for laboratory-grown plants, typically measuring very young plants not grown in soil and not allowing full development of the root system. Results:This s...

journal_title：Plant methods

authors： Chen S,Svane SF,Thorup-Kristensen K

更新日期：2019-12-09 00:00:00

abstract：Background:Progress in high-throughput molecular methods accompanied by more complex experimental designs demands novel data visualisation solutions. To specifically answer the question which parts of the specifical biological system are responding in particular perturbation, integrative approach in which experimental ...

journal_title：Plant methods

authors： Zagorščak M,Blejec A,Ramšak Ž,Petek M,Stare T,Gruden K

更新日期：2018-08-30 00:00:00

abstract：BACKGROUND:When conducting plant research, the measurement of photosynthetic pigments can provide basic information on the physiological status of a plant. High-pressure liquid chromatography (HPLC) is becoming widely used for this purpose because it provides an accurate determination of a variety of photosynthetic pig...

journal_title：Plant methods

authors： Hu X,Tanaka A,Tanaka R

更新日期：2013-06-19 00:00:00

abstract：BACKGROUND:Mitochondria play critical roles in plant growth, development and stress tolerance. Numerous researchers have carried out studies on the plant mitochondrial genome structure, mitochondrial metabolism and nuclear-cytoplasmic interactions. However, classical plant mitochondria extraction methods are time-consu...

journal_title：Plant methods

authors： Xu Y,Li X,Huang J,Peng L,Luo D,Zhang Q,Dan Z,Xiao H,Yang F,Hu J

更新日期：2020-11-04 00:00:00

abstract：Background:Plant parasitic weeds belonging to the genus Striga are a major threat for food production in Sub-Saharan Africa and Southeast Asia. The parasite's life cycle starts with the induction of seed germination by host plant-derived signals, followed by parasite attachment, infection, outgrowth, flowering, reprodu...

journal_title：Plant methods

authors： Masteling R,Voorhoeve L,IJsselmuiden J,Dini-Andreote F,de Boer W,Raaijmakers JM

更新日期：2020-05-01 00:00:00

abstract：Background:Insect herbivory poses a major threat to maize. Benzoxazinoids are important anti-insect secondary metabolites in maize, whose biosynthetic pathway has been extensively studied. However, yet little is known about how benzoxazinoids are regulated in maize, partly due to lack of mutant resources and recalcitra...

journal_title：Plant methods

authors： Gao L,Shen G,Zhang L,Qi J,Zhang C,Ma C,Li J,Wang L,Malook SU,Wu J

更新日期：2019-11-28 00:00:00

abstract：BACKGROUND:A multitude of different imaging systems are already available to image genetically altered RNA species; however, only a few of these techniques are actually suitable to visualize endogenous RNA. One possibility is to use fluorescently-labelled and hybridization-sensitive probes. In order to yield more infor...

journal_title：Plant methods

authors： Göhring J,Fulcher N,Schilcher K,Barta A,Jacak J

更新日期：2014-05-31 00:00:00

abstract：Background:Roses are widely used as garden ornamental plants and cut flowers. Rosa chinensis cv 'Old Blush' has been used as a model genotype in rose studies due to its contribution to recurrent flowering and tea scent traits of modern roses. The deficiency of efficient genetic transformation systems is a handicap limi...

journal_title：Plant methods

authors： Lu J,Bai M,Ren H,Liu J,Wang C

更新日期：2017-12-22 00:00:00

abstract：BACKGROUND:In the contexts of genomics, post-genomics and systems biology approaches, data integration presents a major concern. Databases provide crucial solutions: they store, organize and allow information to be queried, they enhance the visibility of newly produced data by comparing them with previously published r...

journal_title：Plant methods

authors： Dèrozier S,Samson F,Tamby JP,Guichard C,Brunaud V,Grevet P,Gagnot S,Label P,Leplé JC,Lecharny A,Aubourg S

更新日期：2011-03-29 00:00:00

abstract：Background:Plant roots release a variety of organic compounds into the soil which alter the physical, chemical and biological properties of the rhizosphere. Root exudates are technically challenging to measure in soil because roots are difficult to access and exudates can be bound by minerals or consumed by microorgani...

journal_title：Plant methods

authors： Kawasaki A,Okada S,Zhang C,Delhaize E,Mathesius U,Richardson AE,Watt M,Gilliham M,Ryan PR

更新日期：2018-12-20 00:00:00

abstract：BACKGROUND:Protein phosphorylation is accepted as a major regulatory pathway in plants. More than 1000 protein kinases are predicted in the Arabidopsis proteome, however, only a few studies look systematically for in vivo protein phosphorylation sites. Owing to the low stoichiometry and low abundance of phosphorylated ...

journal_title：Plant methods

authors： Wolschin F,Weckwerth W

更新日期：2005-11-01 00:00:00

abstract：Background:Antirrhinum (snapdragon) species are models for genetic and evolutionary research but recalcitrant to genetic transformation, limiting use of transgenic methods for functional genomics. Transient gene expression from viral vectors and virus-induced gene silencing (VIGS) offer transformation-free alternatives...

journal_title：Plant methods

authors： Tan Y,Bukys A,Molnár A,Hudson A

更新日期：2020-10-27 00:00:00

abstract：Background:The demand for effective use of water resources has increased because of ongoing global climate transformations in the agriculture science sector. Cost-effective and timely distributions of the appropriate amount of water are vital not only to maintain a healthy status of plants leaves but to drive the produ...

journal_title：Plant methods

authors： Zahid A,Abbas HT,Ren A,Zoha A,Heidari H,Shah SA,Imran MA,Alomainy A,Abbasi QH

更新日期：2019-11-18 00:00:00

abstract：BACKGROUND:Fluorescent proteins are extraordinary tools for biology studies due to their versatility; they are used extensively to improve comprehension of plant-microbe interactions. The viral infection process can easily be tracked and imaged in a plant with fluorescent protein-tagged viruses. In plants, fluorescent ...

journal_title：Plant methods

authors： Pasin F,Kulasekaran S,Natale P,Simón-Mateo C,García JA

更新日期：2014-07-05 00:00:00

abstract：Background:Image processing techniques have been widely used in the analysis of leaf characteristics. Earlier techniques for processing digital RGB color images of plant leaves had several drawbacks, such as inadequate de-noising, and adopting normal-probability statistical estimation models which have few parameters a...

journal_title：Plant methods

authors： Chen Z,Wang F,Zhang P,Ke C,Zhu Y,Cao W,Jiang H

更新日期：2020-02-26 00:00:00

abstract：Background:The cellular morphology of plant organs is strongly related to other physical properties such as shape, size, growth, mechanical properties or chemical composition. Cell morphology often vary depending on the type of tissue, or on the distance to a specific tissue. A common challenge in quantitative plant hi...

journal_title：Plant methods

authors： Legland D,Guillon F,Devaux MF

更新日期：2020-05-06 00:00:00

abstract：BACKGROUND:Transient gene expression systems are indispensable tools in molecular biology. Yet, their routine application is limited to few plant species often requiring substantial equipment and facilities. High chloroplast and chlorophyll content may further impede downstream applications of transformed cells from gr...

journal_title：Plant methods

authors： Pitzschke A,Persak H

更新日期：2012-05-04 00:00:00

abstract：: We report on a detailed chromatin immunoprecipitation (ChIP) protocol for the unicellular green alga Chlamydomonas reinhardtii. The protocol is suitable for the analysis of nucleosome occupancy, histone modifications and transcription factor binding sites at the level of mononucleosomes for targeted and genome-wide s...

journal_title：Plant methods

authors： Strenkert D,Schmollinger S,Schroda M

更新日期：2011-11-03 00:00:00

abstract：BACKGROUND:There is considerable interest in rapid assays or screening systems for assigning gene function. However, analysis of gene function in the flowers of some species is restricted due to the difficulty of producing stably transformed transgenic plants. As a result, experimental approaches based on transient gen...

journal_title：Plant methods

authors： Shang Y,Schwinn KE,Bennett MJ,Hunter DA,Waugh TL,Pathirana NN,Brummell DA,Jameson PE,Davies KM

更新日期：2007-01-08 00:00:00

abstract：:Primer design is a critical step in the application of PCR-based technologies in gene expression and genetic diversity analysis. As more plant genomes have been sequenced in recent years, the emphasis of primer design strategy has shifted to genome-wide and high-throughput direction. This paper summarizes recent advan...

journal_title：Plant methods

authors： Yang X,Scheffler BE,Weston LA

更新日期：2006-03-01 00:00:00

abstract：:Over the last 10 years, high-density SNP arrays and DNA re-sequencing have illuminated the majority of the genotypic space for a number of organisms, including humans, maize, rice and Arabidopsis. For any researcher willing to define and score a phenotype across many individuals, Genome Wide Association Studies (GWAS)...

journal_title：Plant methods

authors： Korte A,Farlow A

更新日期：2013-07-22 00:00:00