Abstract：
: We report on a detailed chromatin immunoprecipitation (ChIP) protocol for the unicellular green alga Chlamydomonas reinhardtii. The protocol is suitable for the analysis of nucleosome occupancy, histone modifications and transcription factor binding sites at the level of mononucleosomes for targeted and genome-wide studies. We describe the optimization of conditions for crosslinking, chromatin fragmentation and antibody titer determination and provide recommendations and an example for the normalization of ChIP results as determined by real-time PCR.
journal_name
Plant Methodsjournal_title
Plant methodsauthors
Strenkert D,Schmollinger S,Schroda Mdoi
10.1186/1746-4811-7-35subject
Has Abstractpub_date
2011-11-03 00:00:00pages
35issue
1issn
1746-4811pii
1746-4811-7-35journal_volume
7pub_type
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