abstract：BACKGROUND:Maize haploid breeding technology can be used to rapidly develop homozygous lines, significantly shorten the breeding cycle and improve breeding efficiency. Rapid and accurate sorting haploid kernels is a prerequisite for the large-scale application of this technology. At present, the automatic haploid sorti...

journal_title：Plant methods

authors： Qu Y,Liu Z,Zhang Y,Yang J,Li H

更新日期：2021-01-06 00:00:00

abstract：BACKGROUND:Pentatricopeptide-repeat proteins (PPRs) characterized by tandem arrays of a degenerate 35-amino-acid repeat (PPR motif) can bind a single strand RNA and regulate organelle gene expression at the post-transcriptional level, including RNA cleavage, splicing, editing and stability etc. PPRs are conserved in al...

journal_title：Plant methods

authors： Chen J,Zhu H,Huang J,Huang W

更新日期：2020-11-18 00:00:00

abstract：BACKGROUND:Mitochondria play critical roles in plant growth, development and stress tolerance. Numerous researchers have carried out studies on the plant mitochondrial genome structure, mitochondrial metabolism and nuclear-cytoplasmic interactions. However, classical plant mitochondria extraction methods are time-consu...

journal_title：Plant methods

authors： Xu Y,Li X,Huang J,Peng L,Luo D,Zhang Q,Dan Z,Xiao H,Yang F,Hu J

更新日期：2020-11-04 00:00:00

abstract：Background:Antirrhinum (snapdragon) species are models for genetic and evolutionary research but recalcitrant to genetic transformation, limiting use of transgenic methods for functional genomics. Transient gene expression from viral vectors and virus-induced gene silencing (VIGS) offer transformation-free alternatives...

journal_title：Plant methods

authors： Tan Y,Bukys A,Molnár A,Hudson A

更新日期：2020-10-27 00:00:00

abstract：:Plants are good sources of biologically active compounds with antimicrobial activity, including polypeptides. Antimicrobial peptides (AMPs) represent one of the main barriers of plant innate immunity to environmental stress factors and are attracting much research interest. There are some extraction methods for isolat...

journal_title：Plant methods

authors： Barashkova AS,Rogozhin EA

更新日期：2020-10-23 00:00:00

abstract：Background:Chloroplasts are critical organelles that perceive and convey metabolic and stress signals to different cellular components, while remaining the seat of photosynthesis and a metabolic factory. The proteomes of intact leaves, chloroplasts, and suborganellar fractions of plastids have been evaluated in the mod...

journal_title：Plant methods

authors： Bhattacharya O,Ortiz I,Walling LL

更新日期：2020-09-24 00:00:00

abstract：Background:Strigolactones represent the most recently described group of plant hormones involved in many aspects of plant growth regulation. Simultaneously, root exuded strigolactones mediate rhizosphere signaling towards beneficial arbuscular mycorrhizal fungi, but also attract parasitic plants. The seed germination o...

journal_title：Plant methods

authors： Floková K,Shimels M,Andreo Jimenez B,Bardaro N,Strnad M,Novák O,Bouwmeester HJ

更新日期：2020-09-17 00:00:00

abstract：Background:Monitoring the timing of seedling emergence and early development via high-throughput phenotyping with computer vision is a challenging topic of high interest in plant science. While most studies focus on the measurements of leaf area index or detection of specific events such as emergence, little attention ...

journal_title：Plant methods

authors： Samiei S,Rasti P,Ly Vu J,Buitink J,Rousseau D

更新日期：2020-07-30 00:00:00

abstract：Motivation:In 2005, researchers from the French National Research Institute for Agriculture, Food and Environment (Institut national de recherche pour l'agriculture, l'alimentation et l'environnement, INRAE) started a collaboration with the French farmers' seed network Réseau Semences Paysannes (RSP) on bread wheat par...

journal_title：Plant methods

authors： De Oliveira Y,Burlot L,Dawson JC,Goldringer I,Madi D,Rivière P,Steinbach D,van Frank G,Thomas M

更新日期：2020-07-23 00:00:00

abstract：Background:The cellular morphology of plant organs is strongly related to other physical properties such as shape, size, growth, mechanical properties or chemical composition. Cell morphology often vary depending on the type of tissue, or on the distance to a specific tissue. A common challenge in quantitative plant hi...

journal_title：Plant methods

authors： Legland D,Guillon F,Devaux MF

更新日期：2020-05-06 00:00:00

abstract：Background:Plant parasitic weeds belonging to the genus Striga are a major threat for food production in Sub-Saharan Africa and Southeast Asia. The parasite's life cycle starts with the induction of seed germination by host plant-derived signals, followed by parasite attachment, infection, outgrowth, flowering, reprodu...

journal_title：Plant methods

authors： Masteling R,Voorhoeve L,IJsselmuiden J,Dini-Andreote F,de Boer W,Raaijmakers JM

更新日期：2020-05-01 00:00:00

abstract：Background:Functional genomic studies using genetics approaches of conifers are hampered by the complex and enormous genome, long vegetative growth period, and exertion in genetic transformation. Thus, the research carried out on gene function in Pinus tabuliformis is typically performed by heterologous expression base...

journal_title：Plant methods

authors： Liu S,Ma J,Liu H,Guo Y,Li W,Niu S

更新日期：2020-04-10 00:00:00

abstract：Background:Measurements of rind and culm thickness and stem radius/diameter are important to biomechanical, ecological and physiological plant studies. However, many methods of measuring rind thickness and diameter are labor intensive and induce plant fatality. A novel rind puncture methodology for obtaining measuremen...

journal_title：Plant methods

authors： Seegmiller WH,Graves J,Robertson DJ

更新日期：2020-04-01 00:00:00

abstract：Background:High throughput non-destructive phenotyping is emerging as a significant approach for phenotyping germplasm and breeding populations for the identification of superior donors, elite lines, and QTLs. Detection and counting of spikes, the grain bearing organs of wheat, is critical for phenomics of a large set ...

journal_title：Plant methods

authors： Misra T,Arora A,Marwaha S,Chinnusamy V,Rao AR,Jain R,Sahoo RN,Ray M,Kumar S,Raju D,Jha RR,Nigam A,Goel S

更新日期：2020-03-18 00:00:00

abstract：Background:To investigate potential effects of herbicide phytotoxic on crops, a major challenge is a lack of non-destructive and rapid methods to detect plant growth that could allow characterization of herbicide-resistant plants. In such a case, hyperspectral imaging can quickly obtain the spectrum for each pixel in t...

journal_title：Plant methods

authors： Wang J,Zhang C,Shi Y,Long M,Islam F,Yang C,Yang S,He Y,Zhou W

更新日期：2020-03-05 00:00:00

abstract：Background:Image processing techniques have been widely used in the analysis of leaf characteristics. Earlier techniques for processing digital RGB color images of plant leaves had several drawbacks, such as inadequate de-noising, and adopting normal-probability statistical estimation models which have few parameters a...

journal_title：Plant methods

authors： Chen Z,Wang F,Zhang P,Ke C,Zhu Y,Cao W,Jiang H

更新日期：2020-02-26 00:00:00

abstract：Background:Herbaria are valuable sources of extensive curated plant material that are now accessible to genetic studies because of advances in high-throughput, next-generation sequencing methods. As an applied assessment of large-scale recovery of plastid and ribosomal genome sequences from herbarium material for plant...

journal_title：Plant methods

authors： Nevill PG,Zhong X,Tonti-Filippini J,Byrne M,Hislop M,Thiele K,van Leeuwen S,Boykin LM,Small I

更新日期：2020-01-04 00:00:00

abstract：Background:Over the last years, several plant science labs have started to employ fluctuating growth light conditions to simulate natural light regimes more closely. Many plant mutants reveal quantifiable effects under fluctuating light despite being indistinguishable from wild-type plants under standard constant light...

journal_title：Plant methods

authors： Schneider D,Lopez LS,Li M,Crawford JD,Kirchhoff H,Kunz HH

更新日期：2019-12-23 00:00:00

abstract：Background:Deep rooting is one of the most promising plant traits for improving crop yield under water-limited conditions. Most root phenotyping methods are designed for laboratory-grown plants, typically measuring very young plants not grown in soil and not allowing full development of the root system. Results:This s...

journal_title：Plant methods

authors： Chen S,Svane SF,Thorup-Kristensen K

更新日期：2019-12-09 00:00:00

abstract：Background:Insect herbivory poses a major threat to maize. Benzoxazinoids are important anti-insect secondary metabolites in maize, whose biosynthetic pathway has been extensively studied. However, yet little is known about how benzoxazinoids are regulated in maize, partly due to lack of mutant resources and recalcitra...

journal_title：Plant methods

authors： Gao L,Shen G,Zhang L,Qi J,Zhang C,Ma C,Li J,Wang L,Malook SU,Wu J

更新日期：2019-11-28 00:00:00

abstract：Background:The demand for effective use of water resources has increased because of ongoing global climate transformations in the agriculture science sector. Cost-effective and timely distributions of the appropriate amount of water are vital not only to maintain a healthy status of plants leaves but to drive the produ...

journal_title：Plant methods

authors： Zahid A,Abbas HT,Ren A,Zoha A,Heidari H,Shah SA,Imran MA,Alomainy A,Abbasi QH

更新日期：2019-11-18 00:00:00

abstract：Background:Non-contact resonant ultrasound spectroscopy (NC-RUS) has been proven as a reliable technique for the dynamic determination of leaf water status. It has been already tested in more than 50 plant species. In parallel, relative water content (RWC) is highly used in the ecophysiological field to describe the de...

journal_title：Plant methods

authors： Fariñas MD,Jimenez-Carretero D,Sancho-Knapik D,Peguero-Pina JJ,Gil-Pelegrín E,Gómez Álvarez-Arenas T

更新日期：2019-11-07 00:00:00

abstract：Background:Assessing the impact of the environment on plant performance requires growing plants under controlled environmental conditions. Plant phenotypes are a product of genotype × environment (G × E), and the Enviratron at Iowa State University is a facility for testing under controlled conditions the effects of th...

journal_title：Plant methods

authors： Bao Y,Zarecor S,Shah D,Tuel T,Campbell DA,Chapman AVE,Imberti D,Kiekhaefer D,Imberti H,Lübberstedt T,Yin Y,Nettleton D,Lawrence-Dill CJ,Whitham SA,Tang L,Howell SH

更新日期：2019-10-23 00:00:00

abstract：:[This corrects the article DOI: 10.1186/s13007-019-0478-9.]. ...

journal_title：Plant methods

authors： Dobbels AA,Lorenz AJ

更新日期：2019-10-10 00:00:00

abstract：Background:An effective and expeditious approach to assess plant nitrogen status is urgently needed in rice production and management as the conventional chemical methods are laborious and time-consuming. Results:Fourier transform infrared photoacoustic spectroscopy (FTIR-PAS) was used to record the spectra of rice le...

journal_title：Plant methods

authors： Wu K,Du C,Ma F,Shen Y,Liang D,Zhou J

更新日期：2019-08-19 00:00:00

abstract：Background:Most bacteria are not culturable, but can be identified through molecular methods such as metagenomics studies. Due to specific metabolic requirements and symbiotic relationships, these bacteria cannot survive on typical laboratory media. Many economically and medically important bacteria are unculturable; i...

journal_title：Plant methods

authors： Krystel J,Shi Q,Shaw J,Gupta G,Hall D,Stover E

更新日期：2019-07-31 00:00:00

abstract：Background:In-field measurement of yield and growth rate in pasture species is imprecise and costly, limiting scientific and commercial application. Our study proposed a LiDAR-based mobile platform for non-invasive vegetative biomass and growth rate estimation in perennial ryegrass (Lolium perenne L.). This included de...

journal_title：Plant methods

authors： Ghamkhar K,Irie K,Hagedorn M,Hsiao J,Fourie J,Gebbie S,Hoyos-Villegas V,George R,Stewart A,Inch C,Werner A,Barrett B

更新日期：2019-07-10 00:00:00

abstract：Background:Protein data over circadian time scale is scarce for clock transcription factors. Further work in this direction is required for refining quantitative clock models. However, gathering highly resolved dynamics of low-abundance transcription factors has been a major challenge in the field. In this work we prov...

journal_title：Plant methods

authors： Urquiza-García U,Millar AJ

更新日期：2019-07-08 00:00:00

abstract：Background:The stomata of plants mainly regulate gas exchange and water dispersion between the interior and external environments of plants and play a major role in the plants' health. The existing methods of stomata segmentation and measurement are mostly for specialized plants. The purpose of this research is to deve...

journal_title：Plant methods

authors： Li K,Huang J,Song W,Wang J,Lv S,Wang X

更新日期：2019-07-03 00:00:00

abstract：Background:Woody tropical plants contain high levels of complex organic compounds that inhibit the chemical procedures needed to extract RNA or DNA, thus compromising downstream applications such as RNA sequencing and analysis of gene expression. To overcome this issue, researchers must use extraction protocols using C...

journal_title：Plant methods

authors： Barbier FF,Chabikwa TG,Ahsan MU,Cook SE,Powell R,Tanurdzic M,Beveridge CA

更新日期：2019-06-05 00:00:00

abstract：Background:Plastome (plastid genome) sequences provide valuable information for understanding the phylogenetic relationships and evolutionary history of plants. Although the rapid development of high-throughput sequencing technology has led to an explosion of plastome sequences, annotation remains a significant bottlen...

journal_title：Plant methods

authors： Qu XJ,Moore MJ,Li DZ,Yi TS

更新日期：2019-05-21 00:00:00

abstract：Background:Many agronomic traits have been bred into modern wheat varieties, but wheat (Triticum aestivum L.) continues to be vulnerable to heat stress, with high night-time temperature (HNT) stress shown to have large negative impact on yield and quality. Global mean temperature during the day is consistently warming ...

journal_title：Plant methods

authors： Hein NT,Wagner D,Bheemanahalli R,Šebela D,Bustamante C,Chiluwal A,Neilsen ML,Jagadish SVK

更新日期：2019-04-24 00:00:00

abstract：Background:Root is the principal part of plants to absorb water and nutrient, anchor the plant and affect yield and quality. Non-destructive detection of root traits is difficult to perform due to the hidden nature of the root. Therefore, improved methods to measure roots are necessary to support plant breeding, and op...

journal_title：Plant methods

authors： Lu W,Wang X,Wang F

更新日期：2019-03-23 00:00:00

abstract：Background:Nowadays, there is an increasing demand for fast and reliable plant biomolecular analyses. Conventional methods for the isolation of nucleic acids are time-consuming and require multiple and often non-automatable steps to remove cellular interferences, with consequence that sample preparation is the major bo...

journal_title：Plant methods

authors： Marengo A,Cagliero C,Sgorbini B,Anderson JL,Emaus MN,Bicchi C,Bertea CM,Rubiolo P

更新日期：2019-03-09 00:00:00

abstract：Background:Plant root apex is the major part to direct the root growth and development by responding to various signals/cues from internal and soil environments. To study and understand root system biology particularly at a molecular and cellular level, an Arabidopsis T-DNA insertional enhancer trap line J3411 expressi...

journal_title：Plant methods

authors： Zhang L,Qin LN,Zeng ZR,Wu CZ,Gong YY,Liu LH,Cao FQ

更新日期：2019-01-31 00:00:00

abstract：Background:Open tissue culture technique could be simplified by using different bacteriostatic agents. There is a great difference in the bacteriostatic effects of different antimicrobial agents on various explants. However, there is no report about the effective bacteriostatic agent for open tissue culture of Acacia a...

journal_title：Plant methods

authors： Huang L,Wang H,Shahid MQ,Zeng B

更新日期：2019-01-25 00:00:00

abstract：Background:Plant roots release a variety of organic compounds into the soil which alter the physical, chemical and biological properties of the rhizosphere. Root exudates are technically challenging to measure in soil because roots are difficult to access and exudates can be bound by minerals or consumed by microorgani...

journal_title：Plant methods

authors： Kawasaki A,Okada S,Zhang C,Delhaize E,Mathesius U,Richardson AE,Watt M,Gilliham M,Ryan PR

更新日期：2018-12-20 00:00:00

abstract：Background:Plasmopara obducens is the biotrophic oomycete responsible for impatiens downy mildew, a destructive disease of Impatiens that causes high crop loss. Currently, there are no available methods for the microscopic detection of P. obducens from leaves of impatiens, which may be contributing to the spread of the...

journal_title：Plant methods

authors： Salgado-Salazar C,Bauchan GR,Wallace EC,Crouch JA

更新日期：2018-10-24 00:00:00

abstract：Background:Charcoal rot is a fungal disease that thrives in warm dry conditions and affects the yield of soybeans and other important agronomic crops worldwide. There is a need for robust, automatic and consistent early detection and quantification of disease symptoms which are important in breeding programs for the de...

journal_title：Plant methods

authors： Nagasubramanian K,Jones S,Sarkar S,Singh AK,Singh A,Ganapathysubramanian B

更新日期：2018-10-03 00:00:00

abstract：Background:Progress in high-throughput molecular methods accompanied by more complex experimental designs demands novel data visualisation solutions. To specifically answer the question which parts of the specifical biological system are responding in particular perturbation, integrative approach in which experimental ...

journal_title：Plant methods

authors： Zagorščak M,Blejec A,Ramšak Ž,Petek M,Stare T,Gruden K

更新日期：2018-08-30 00:00:00