Microscopy of Physcomitrella patens sperm cells.

abstract：BACKGROUND:In silico analyses based on sequence similarities with animal channels have identified a large number of plant genes likely to encode ion channels. The attempts made to characterise such putative plant channels at the functional level have most often relied on electrophysiological analyses in classical expre...

journal_title：Plant methods

authors： Hosy E,Duby G,Véry AA,Costa A,Sentenac H,Thibaud JB

更新日期：2005-12-19 00:00:00

abstract：:[This corrects the article DOI: 10.1186/s13007-017-0166-6.]. ...

journal_title：Plant methods

authors： Lüpke M,Steinbrecher R,Leuchner M,Menzel A

更新日期：2017-11-16 00:00:00

abstract：BACKGROUND:Simultaneous analysis of multiple functional-related phytohormones and their metabolites will improve our understanding of interactions among different hormones in the same biologic process. RESULTS:A method was developed for simultaneous quantification of multiple phytohormones, abscisic acid, indole-3-ace...

journal_title：Plant methods

authors： Liu H,Li X,Xiao J,Wang S

更新日期：2012-01-15 00:00:00

abstract：BACKGROUND:Nectar reabsorption is a widely known phenomenon, related to the strategy of resource-recovery and also to maintain the nectar homeostasis at the nectary. The method currently performed to demonstrate nectar being reabsorbed involves the use of radioactive tracers applied to the nectary. Although this method...

journal_title：Plant methods

authors： Cardoso-Gustavson P,Robazzi Bignelli Valente Aguiar JM,Ricardo Pansarin E,de Barros F

更新日期：2013-06-19 00:00:00

abstract：: Three terrestrial plants are known to perform C4 photosynthesis without the dual-cell system by partitioning two distinct types of chloroplasts in separate cytoplasmic compartments. We report herein a protocol for isolating the dimorphic chloroplasts from Bienertia sinuspersici. Hypo-osmotically lysed protoplasts und...

journal_title：Plant methods

authors： Lung SC,Yanagisawa M,Chuong SD

更新日期：2012-03-06 00:00:00

abstract：BACKGROUND:The main aim of this study was to improve fungal resistance in bread wheat via transgenesis. Transgenic wheat plants harboring barley chitinase (chi26) gene, driven by maize ubi promoter, were obtained using biolistic bombardment, whereas the herbicide resistance gene, bar, driven by the CaMV 35S promoter wa...

journal_title：Plant methods

authors： Eissa HF,Hassanien SE,Ramadan AM,El-Shamy MM,Saleh OM,Shokry AM,Abdelsattar M,Morsy YB,El-Maghraby MA,Alameldin HF,Hassan SM,Osman GH,Mahfouz HT,Gad El-Karim GA,Madkour MA,Bahieldin A

更新日期：2017-05-22 00:00:00

abstract：BACKGROUND:In eukaryotic cells, the proteasome maintains homeostasis by selectively degrading regulatory and misfolded proteins, and in doing so contributes to the amino acid pool. Inhibition of the proteasome in yeast and human cells decreases de novo protein synthesis. However, it is not know if proteasome inhibition...

journal_title：Plant methods

authors： Van Hoewyk D

更新日期：2016-03-16 00:00:00

abstract：BACKGROUND:The data generated during a course of a biological experiment/study can be sometimes be massive and its management becomes quite critical for the success of the investigation undertaken. The accumulation and analysis of such large datasets often becomes tedious for biologists and lab technicians. Most of the...

journal_title：Plant methods

authors： Vankadavath RN,Hussain AJ,Bodanapu R,Kharshiing E,Basha PO,Gupta S,Sreelakshmi Y,Sharma R

更新日期：2009-12-10 00:00:00

abstract：:Intact protein complexes can be separated by apparent molecular mass using a standard polyacrylamide gel electrophoresis system combining mild detergents and the dye Coomassie Blue. Referring to the blue coloured gel and the gentle method of solubilization yielding native and enzymatically active protein complexes, th...

journal_title：Plant methods

authors： Eubel H,Braun HP,Millar AH

更新日期：2005-11-16 00:00:00

abstract：Background:The cellular morphology of plant organs is strongly related to other physical properties such as shape, size, growth, mechanical properties or chemical composition. Cell morphology often vary depending on the type of tissue, or on the distance to a specific tissue. A common challenge in quantitative plant hi...

journal_title：Plant methods

authors： Legland D,Guillon F,Devaux MF

更新日期：2020-05-06 00:00:00

abstract：BACKGROUND:Establishment and maintenance of mutualistic plant-microbial interactions in the rhizosphere and within plant roots involve several root cell types. The processes of host-microbe recognition and infection require complex signal exchange and activation of downstream responses. These molecular events coordinat...

journal_title：Plant methods

authors： Gavrilovic S,Yan Z,Jurkiewicz AM,Stougaard J,Markmann K

更新日期：2016-01-22 00:00:00

abstract：: Large-scale genetic screens in Arabidopsis are a powerful approach for molecular dissection of complex signaling networks. However, map-based cloning can be time-consuming or even hampered due to low chromosomal recombination. Current strategies using next generation sequencing for molecular identification of mutatio...

journal_title：Plant methods

authors： Liu KH,McCormack M,Sheen J

更新日期：2012-03-30 00:00:00

abstract：BACKGROUND:Plant breeding and genetics demand fast, exact and reproducible phenotyping. Efficient statistical evaluation of phenotyping data requires standardised data storage ensuring long-term data availability while maintaining intellectual property rights. This is state of the art at phenomics centres, which, howev...

journal_title：Plant methods

authors： Köhl K,Gremmels J

更新日期：2015-04-07 00:00:00

abstract：BACKGROUND:Leaf rust (LR), caused by Puccinia triticina and is an important disease of wheat (Triticum aestivum L.). The most sustainable method for controlling rust diseases is deployment of cultivars incorporating adult plant resistance (APR). However, phenotyping breeding populations or germplasm collections for res...

journal_title：Plant methods

authors： Riaz A,Periyannan S,Aitken E,Hickey L

更新日期：2016-03-02 00:00:00

abstract：Background:Many agronomic traits have been bred into modern wheat varieties, but wheat (Triticum aestivum L.) continues to be vulnerable to heat stress, with high night-time temperature (HNT) stress shown to have large negative impact on yield and quality. Global mean temperature during the day is consistently warming ...

journal_title：Plant methods

authors： Hein NT,Wagner D,Bheemanahalli R,Šebela D,Bustamante C,Chiluwal A,Neilsen ML,Jagadish SVK

更新日期：2019-04-24 00:00:00

abstract：BACKGROUND:Protein phosphorylation is accepted as a major regulatory pathway in plants. More than 1000 protein kinases are predicted in the Arabidopsis proteome, however, only a few studies look systematically for in vivo protein phosphorylation sites. Owing to the low stoichiometry and low abundance of phosphorylated ...

journal_title：Plant methods

authors： Wolschin F,Weckwerth W

更新日期：2005-11-01 00:00:00

abstract：BACKGROUND:Brassinosteriods (BRs) are a group of important phytohormones that have major effects on plant growth and development. To fully elucidate the function of BRs, a sensitive BR assay is required. However, most of the previously reported methods are tedious and time-consuming due to multiple pretreatment steps. ...

journal_title：Plant methods

authors： Ding J,Wu JH,Liu JF,Yuan BF,Feng YQ

更新日期：2014-11-24 00:00:00

abstract：Background:Herbaria are valuable sources of extensive curated plant material that are now accessible to genetic studies because of advances in high-throughput, next-generation sequencing methods. As an applied assessment of large-scale recovery of plastid and ribosomal genome sequences from herbarium material for plant...

journal_title：Plant methods

authors： Nevill PG,Zhong X,Tonti-Filippini J,Byrne M,Hislop M,Thiele K,van Leeuwen S,Boykin LM,Small I

更新日期：2020-01-04 00:00:00

abstract：Background:Progress in high-throughput molecular methods accompanied by more complex experimental designs demands novel data visualisation solutions. To specifically answer the question which parts of the specifical biological system are responding in particular perturbation, integrative approach in which experimental ...

journal_title：Plant methods

authors： Zagorščak M,Blejec A,Ramšak Ž,Petek M,Stare T,Gruden K

更新日期：2018-08-30 00:00:00

abstract：Background:Plant roots release a variety of organic compounds into the soil which alter the physical, chemical and biological properties of the rhizosphere. Root exudates are technically challenging to measure in soil because roots are difficult to access and exudates can be bound by minerals or consumed by microorgani...

journal_title：Plant methods

authors： Kawasaki A,Okada S,Zhang C,Delhaize E,Mathesius U,Richardson AE,Watt M,Gilliham M,Ryan PR

更新日期：2018-12-20 00:00:00

abstract：Background:Open tissue culture technique could be simplified by using different bacteriostatic agents. There is a great difference in the bacteriostatic effects of different antimicrobial agents on various explants. However, there is no report about the effective bacteriostatic agent for open tissue culture of Acacia a...

journal_title：Plant methods

authors： Huang L,Wang H,Shahid MQ,Zeng B

更新日期：2019-01-25 00:00:00

abstract：BACKGROUND:Effects of abiotic and biotic stresses on plant photosynthetic performance lead to fitness and yield decrease. The maximum quantum efficiency of photosystem II (F v/F m) is a parameter of chlorophyll fluorescence (ChlF) classically used to track changes in photosynthetic performance. Despite recent technical...

journal_title：Plant methods

authors： Bresson J,Vasseur F,Dauzat M,Koch G,Granier C,Vile D

更新日期：2015-03-26 00:00:00

abstract：Background:Wheat is one of the most widely grown crop in temperate climates for food and animal feed. In order to meet the demands of the predicted population increase in an ever-changing climate, wheat production needs to dramatically increase. Spike and grain traits are critical determinants of final yield and grain ...

journal_title：Plant methods

authors： Hughes N,Askew K,Scotson CP,Williams K,Sauze C,Corke F,Doonan JH,Nibau C

更新日期：2017-11-01 00:00:00

abstract：Motivation:In 2005, researchers from the French National Research Institute for Agriculture, Food and Environment (Institut national de recherche pour l'agriculture, l'alimentation et l'environnement, INRAE) started a collaboration with the French farmers' seed network Réseau Semences Paysannes (RSP) on bread wheat par...

journal_title：Plant methods

authors： De Oliveira Y,Burlot L,Dawson JC,Goldringer I,Madi D,Rivière P,Steinbach D,van Frank G,Thomas M

更新日期：2020-07-23 00:00:00

abstract：Background:Chickpea (Cicer arietinum L.), an important legume crop is one of the major source of dietary protein. Developing an efficient and reproducible transformation method is imperative to expedite functional genomics studies in this crop. Here, we present an optimized and detailed procedure for Agrobacterium rhiz...

journal_title：Plant methods

authors： Aggarwal PR,Nag P,Choudhary P,Chakraborty N,Chakraborty S

更新日期：2018-07-06 00:00:00

abstract：BACKGROUND:Despite increasing demand, imaging the internal structure of plant organs or tissues without the use of transgenic lines expressing fluorescent proteins remains a challenge. Techniques such as magnetic resonance imaging, optical projection tomography or X-ray absorption tomography have been used with various...

journal_title：Plant methods

authors： Rousseau D,Widiez T,Di Tommaso S,Rositi H,Adrien J,Maire E,Langer M,Olivier C,Peyrin F,Rogowsky P

更新日期：2015-12-18 00:00:00

abstract：BACKGROUND:It is well known that preparation of biological (plant and animal) tissues for Scanning Electron Microscopy (SEM) by chemical fixation and critical point drying results in shrinkage of tissues, often by up to 20-30%, depending on the tissue type and fixation protocol used. We sought to identify a protocol th...

journal_title：Plant methods

authors： Talbot MJ,White RG

更新日期：2013-10-02 00:00:00

abstract：Background:Over the last years, several plant science labs have started to employ fluctuating growth light conditions to simulate natural light regimes more closely. Many plant mutants reveal quantifiable effects under fluctuating light despite being indistinguishable from wild-type plants under standard constant light...

journal_title：Plant methods

authors： Schneider D,Lopez LS,Li M,Crawford JD,Kirchhoff H,Kunz HH

更新日期：2019-12-23 00:00:00

abstract：Background:Insect herbivory poses a major threat to maize. Benzoxazinoids are important anti-insect secondary metabolites in maize, whose biosynthetic pathway has been extensively studied. However, yet little is known about how benzoxazinoids are regulated in maize, partly due to lack of mutant resources and recalcitra...

journal_title：Plant methods

authors： Gao L,Shen G,Zhang L,Qi J,Zhang C,Ma C,Li J,Wang L,Malook SU,Wu J

更新日期：2019-11-28 00:00:00

abstract：BACKGROUND:There is considerable interest in rapid assays or screening systems for assigning gene function. However, analysis of gene function in the flowers of some species is restricted due to the difficulty of producing stably transformed transgenic plants. As a result, experimental approaches based on transient gen...

journal_title：Plant methods

authors： Shang Y,Schwinn KE,Bennett MJ,Hunter DA,Waugh TL,Pathirana NN,Brummell DA,Jameson PE,Davies KM

更新日期：2007-01-08 00:00:00