Adaptive minirhizotron for pepper roots observation and its installation based on root system architecture traits.

abstract：Background:Over the last years, several plant science labs have started to employ fluctuating growth light conditions to simulate natural light regimes more closely. Many plant mutants reveal quantifiable effects under fluctuating light despite being indistinguishable from wild-type plants under standard constant light...

journal_title：Plant methods

authors： Schneider D,Lopez LS,Li M,Crawford JD,Kirchhoff H,Kunz HH

更新日期：2019-12-23 00:00:00

abstract：Background:Deep rooting is one of the most promising plant traits for improving crop yield under water-limited conditions. Most root phenotyping methods are designed for laboratory-grown plants, typically measuring very young plants not grown in soil and not allowing full development of the root system. Results:This s...

journal_title：Plant methods

authors： Chen S,Svane SF,Thorup-Kristensen K

更新日期：2019-12-09 00:00:00

abstract：Background:Nowadays, there is an increasing demand for fast and reliable plant biomolecular analyses. Conventional methods for the isolation of nucleic acids are time-consuming and require multiple and often non-automatable steps to remove cellular interferences, with consequence that sample preparation is the major bo...

journal_title：Plant methods

authors： Marengo A,Cagliero C,Sgorbini B,Anderson JL,Emaus MN,Bicchi C,Bertea CM,Rubiolo P

更新日期：2019-03-09 00:00:00

abstract：Background:Image processing techniques have been widely used in the analysis of leaf characteristics. Earlier techniques for processing digital RGB color images of plant leaves had several drawbacks, such as inadequate de-noising, and adopting normal-probability statistical estimation models which have few parameters a...

journal_title：Plant methods

authors： Chen Z,Wang F,Zhang P,Ke C,Zhu Y,Cao W,Jiang H

更新日期：2020-02-26 00:00:00

abstract：BACKGROUND:Protein phosphorylation is accepted as a major regulatory pathway in plants. More than 1000 protein kinases are predicted in the Arabidopsis proteome, however, only a few studies look systematically for in vivo protein phosphorylation sites. Owing to the low stoichiometry and low abundance of phosphorylated ...

journal_title：Plant methods

authors： Wolschin F,Weckwerth W

更新日期：2005-11-01 00:00:00

abstract：BACKGROUND:Archegoniates (bryophytes, ferns and gymnosperms), such as the moss Physcomitrella patens, possess freely motile sperm cells (spermatozoids) which reach the egg cell via surface water. Although these motile flagellated sperm cells are a traditional botanical subject, they have not been thoroughly analysed in...

journal_title：Plant methods

authors： Horst NA,Reski R

更新日期：2017-05-10 00:00:00

abstract：BACKGROUND:Despite increasing demand, imaging the internal structure of plant organs or tissues without the use of transgenic lines expressing fluorescent proteins remains a challenge. Techniques such as magnetic resonance imaging, optical projection tomography or X-ray absorption tomography have been used with various...

journal_title：Plant methods

authors： Rousseau D,Widiez T,Di Tommaso S,Rositi H,Adrien J,Maire E,Langer M,Olivier C,Peyrin F,Rogowsky P

更新日期：2015-12-18 00:00:00

abstract：BACKGROUND:Effects of abiotic and biotic stresses on plant photosynthetic performance lead to fitness and yield decrease. The maximum quantum efficiency of photosystem II (F v/F m) is a parameter of chlorophyll fluorescence (ChlF) classically used to track changes in photosynthetic performance. Despite recent technical...

journal_title：Plant methods

authors： Bresson J,Vasseur F,Dauzat M,Koch G,Granier C,Vile D

更新日期：2015-03-26 00:00:00

abstract：Background:An effective and expeditious approach to assess plant nitrogen status is urgently needed in rice production and management as the conventional chemical methods are laborious and time-consuming. Results:Fourier transform infrared photoacoustic spectroscopy (FTIR-PAS) was used to record the spectra of rice le...

journal_title：Plant methods

authors： Wu K,Du C,Ma F,Shen Y,Liang D,Zhou J

更新日期：2019-08-19 00:00:00

abstract：BACKGROUND:Nectar reabsorption is a widely known phenomenon, related to the strategy of resource-recovery and also to maintain the nectar homeostasis at the nectary. The method currently performed to demonstrate nectar being reabsorbed involves the use of radioactive tracers applied to the nectary. Although this method...

journal_title：Plant methods

authors： Cardoso-Gustavson P,Robazzi Bignelli Valente Aguiar JM,Ricardo Pansarin E,de Barros F

更新日期：2013-06-19 00:00:00

abstract：BACKGROUND:Plant breeding and genetics demand fast, exact and reproducible phenotyping. Efficient statistical evaluation of phenotyping data requires standardised data storage ensuring long-term data availability while maintaining intellectual property rights. This is state of the art at phenomics centres, which, howev...

journal_title：Plant methods

authors： Köhl K,Gremmels J

更新日期：2015-04-07 00:00:00

abstract：Background:Plasmopara obducens is the biotrophic oomycete responsible for impatiens downy mildew, a destructive disease of Impatiens that causes high crop loss. Currently, there are no available methods for the microscopic detection of P. obducens from leaves of impatiens, which may be contributing to the spread of the...

journal_title：Plant methods

authors： Salgado-Salazar C,Bauchan GR,Wallace EC,Crouch JA

更新日期：2018-10-24 00:00:00

abstract：Background:Antirrhinum (snapdragon) species are models for genetic and evolutionary research but recalcitrant to genetic transformation, limiting use of transgenic methods for functional genomics. Transient gene expression from viral vectors and virus-induced gene silencing (VIGS) offer transformation-free alternatives...

journal_title：Plant methods

authors： Tan Y,Bukys A,Molnár A,Hudson A

更新日期：2020-10-27 00:00:00

abstract：BACKGROUND:Plant metabolites are commonly functionally classified, as defense- or growth-related phytohormones, primary and specialized metabolites, and so forth. Analytical procedures for the quantifications of these metabolites are challenging because the metabolites can vary over several orders of magnitude in conce...

journal_title：Plant methods

authors： Schäfer M,Brütting C,Baldwin IT,Kallenbach M

更新日期：2016-05-26 00:00:00

abstract：BACKGROUND:Pollen tubes extend rapidly when pollen grains are incubated in defined media. Tube extension requires many critical functions of plant cells including molecular signaling, cytoskeleton remodeling, secretion, and cell wall synthesis. Consequently, pollen tube growth has been established as a way to conduct p...

journal_title：Plant methods

authors： Hartman E,Levy C,Kern DM,Johnson MA,Basu A

更新日期：2014-01-23 00:00:00

abstract：BACKGROUND:Identifying cis-regulatory elements is critical in understanding the direct and indirect regulatory mechanisms of gene expression. Current approaches include DNase-seq, a technique that combines sensitivity to the nonspecific endonuclease DNase I with high throughput sequencing to identify regions of regulat...

journal_title：Plant methods

authors： Cumbie JS,Filichkin SA,Megraw M

更新日期：2015-09-04 00:00:00

abstract：:Terahertz technology is still an evolving research field that attracts scientists with very different backgrounds working on a wide range of subjects. In the past two decades, it has been demonstrated that terahertz technology can provide a non-invasive tool for measuring and monitoring the water content of leaves and...

journal_title：Plant methods

authors： Gente R,Koch M

更新日期：2015-03-06 00:00:00

abstract：: Generating and identifying transformants is essential for many studies of gene function. In Arabidopsis thaliana, a revolutionary protocol termed floral dip is now the most widely used transformation method. Although robust, it involves a number of relatively time-consuming and laborious steps, including manipulating...

journal_title：Plant methods

authors： Davis AM,Hall A,Millar AJ,Darrah C,Davis SJ

更新日期：2009-02-27 00:00:00

abstract：Background:Measurements of rind and culm thickness and stem radius/diameter are important to biomechanical, ecological and physiological plant studies. However, many methods of measuring rind thickness and diameter are labor intensive and induce plant fatality. A novel rind puncture methodology for obtaining measuremen...

journal_title：Plant methods

authors： Seegmiller WH,Graves J,Robertson DJ

更新日期：2020-04-01 00:00:00

abstract：:[This corrects the article DOI: 10.1186/s13007-017-0166-6.]. ...

journal_title：Plant methods

authors： Lüpke M,Steinbrecher R,Leuchner M,Menzel A

更新日期：2017-11-16 00:00:00

abstract：BACKGROUND:Establishing transcriptional regulatory networks that include protein-protein and protein-DNA interactions has become a key component to better understanding many fundamental biological processes. Although a variety of techniques are available to expose protein-protein and protein-DNA interactions, unequivoc...

journal_title：Plant methods

authors： Xie Z,Grotewold E

更新日期：2008-10-27 00:00:00

abstract：UNLABELLED: BACKGROUND:Phytohormones are the key metabolites participating in the regulation of multiple functions of plant organism. Among them, jasmonates, as well as abscisic and salicylic acids are responsible for triggering and modulating plant reactions targeted against pathogens and herbivores, as well as resis...

journal_title：Plant methods

authors： Balcke GU,Handrick V,Bergau N,Fichtner M,Henning A,Stellmach H,Tissier A,Hause B,Frolov A

更新日期：2012-11-22 00:00:00

abstract：Background:Herbaria are valuable sources of extensive curated plant material that are now accessible to genetic studies because of advances in high-throughput, next-generation sequencing methods. As an applied assessment of large-scale recovery of plastid and ribosomal genome sequences from herbarium material for plant...

journal_title：Plant methods

authors： Nevill PG,Zhong X,Tonti-Filippini J,Byrne M,Hislop M,Thiele K,van Leeuwen S,Boykin LM,Small I

更新日期：2020-01-04 00:00:00

abstract：BACKGROUND:There is considerable interest in rapid assays or screening systems for assigning gene function. However, analysis of gene function in the flowers of some species is restricted due to the difficulty of producing stably transformed transgenic plants. As a result, experimental approaches based on transient gen...

journal_title：Plant methods

authors： Shang Y,Schwinn KE,Bennett MJ,Hunter DA,Waugh TL,Pathirana NN,Brummell DA,Jameson PE,Davies KM

更新日期：2007-01-08 00:00:00

abstract：Background:The cellular morphology of plant organs is strongly related to other physical properties such as shape, size, growth, mechanical properties or chemical composition. Cell morphology often vary depending on the type of tissue, or on the distance to a specific tissue. A common challenge in quantitative plant hi...

journal_title：Plant methods

authors： Legland D,Guillon F,Devaux MF

更新日期：2020-05-06 00:00:00

abstract：BACKGROUND:The elucidation of complex biological systems requires integration of multiple molecular parameters. Accordingly, high throughput methods like transcriptomics, proteomics, metabolomics and lipidomics have emerged to provide the tools for successful system-wide investigations. Unfortunately, optimized analysi...

journal_title：Plant methods

authors： Salem MA,Jüppner J,Bajdzienko K,Giavalisco P

更新日期：2016-11-10 00:00:00

abstract：:Modern agriculture has created a demand for plant biotechnology products that provide durable resistance to insect pests, tolerance of herbicide applications for weed control, and agronomic traits tailored for specific geographies. These transgenic trait products require a modular and sequential multigene stacking pla...

journal_title：Plant methods

authors： Kumar S,Barone P,Smith M

更新日期：2016-02-01 00:00:00

abstract：BACKGROUND:Allotetraploid white clover (Trifolium repens L.) is an important forage legume widely cultivated in most temperate regions. Only a small number of microsatellite markers are publicly available and can be utilized in white clover breeding programs. The objectives of this study were to develop an integrated a...

journal_title：Plant methods

authors： Zhang Y,He J,Zhao PX,Bouton JH,Monteros MJ

更新日期：2008-07-16 00:00:00

abstract：Background:Charcoal rot is a fungal disease that thrives in warm dry conditions and affects the yield of soybeans and other important agronomic crops worldwide. There is a need for robust, automatic and consistent early detection and quantification of disease symptoms which are important in breeding programs for the de...

journal_title：Plant methods

authors： Nagasubramanian K,Jones S,Sarkar S,Singh AK,Singh A,Ganapathysubramanian B

更新日期：2018-10-03 00:00:00

abstract：:Over the last 10 years, high-density SNP arrays and DNA re-sequencing have illuminated the majority of the genotypic space for a number of organisms, including humans, maize, rice and Arabidopsis. For any researcher willing to define and score a phenotype across many individuals, Genome Wide Association Studies (GWAS)...

journal_title：Plant methods

authors： Korte A,Farlow A

更新日期：2013-07-22 00:00:00