Abstract：
: Generating and identifying transformants is essential for many studies of gene function. In Arabidopsis thaliana, a revolutionary protocol termed floral dip is now the most widely used transformation method. Although robust, it involves a number of relatively time-consuming and laborious steps, including manipulating an Agrobacterium tumefaciens culture and aseptic procedures for the selection of plant lines harboring antibiotic-selection markers. Furthermore, where multiple transgenes are to be introduced, achieving this by sequential transformations over multiple generations adds significantly to the time required. To circumvent these bottlenecks, we have developed three streamlined sub-protocols. First, we find that A. thaliana can be transformed by dipping directly into an A. tumefaciens culture supplemented with surfactant, eliminating the need for media exchange to a buffered solution. Next, we illustrate that A. thaliana lines possessing a double-transformation event can be readily generated by simply by floral-dipping into a mixture of two A. tumefaciens cultures harboring distinct transformation vectors. Finally, we report an alternative method of transformant selection on chromatography sand that does not require surface sterilization of seeds. These sub-protocols, which can be used separately or in combination, save time and money, and reduce the possibility of contamination.
journal_name
Plant Methodsjournal_title
Plant methodsauthors
Davis AM,Hall A,Millar AJ,Darrah C,Davis SJdoi
10.1186/1746-4811-5-3subject
Has Abstractpub_date
2009-02-27 00:00:00pages
3issn
1746-4811pii
1746-4811-5-3journal_volume
5pub_type
杂志文章相关文献
Plant Methods文献大全abstract：Background:Open tissue culture technique could be simplified by using different bacteriostatic agents. There is a great difference in the bacteriostatic effects of different antimicrobial agents on various explants. However, there is no report about the effective bacteriostatic agent for open tissue culture of Acacia a...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/s13007-019-0390-3
更新日期：2019-01-25 00:00:00
abstract：BACKGROUND:In silico analyses based on sequence similarities with animal channels have identified a large number of plant genes likely to encode ion channels. The attempts made to characterise such putative plant channels at the functional level have most often relied on electrophysiological analyses in classical expre...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/1746-4811-1-14
更新日期：2005-12-19 00:00:00
abstract：Background:Root is the principal part of plants to absorb water and nutrient, anchor the plant and affect yield and quality. Non-destructive detection of root traits is difficult to perform due to the hidden nature of the root. Therefore, improved methods to measure roots are necessary to support plant breeding, and op...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/s13007-019-0414-z
更新日期：2019-03-23 00:00:00
abstract：:Modern agriculture has created a demand for plant biotechnology products that provide durable resistance to insect pests, tolerance of herbicide applications for weed control, and agronomic traits tailored for specific geographies. These transgenic trait products require a modular and sequential multigene stacking pla...
journal_title：Plant methods
pub_type： 杂志文章,评审
doi：10.1186/s13007-016-0111-0
更新日期：2016-02-01 00:00:00
abstract：:Terahertz technology is still an evolving research field that attracts scientists with very different backgrounds working on a wide range of subjects. In the past two decades, it has been demonstrated that terahertz technology can provide a non-invasive tool for measuring and monitoring the water content of leaves and...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/s13007-015-0057-7
更新日期：2015-03-06 00:00:00
abstract：BACKGROUND:Mitochondria play critical roles in plant growth, development and stress tolerance. Numerous researchers have carried out studies on the plant mitochondrial genome structure, mitochondrial metabolism and nuclear-cytoplasmic interactions. However, classical plant mitochondria extraction methods are time-consu...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/s13007-020-00690-6
更新日期：2020-11-04 00:00:00
abstract：BACKGROUND:A multitude of different imaging systems are already available to image genetically altered RNA species; however, only a few of these techniques are actually suitable to visualize endogenous RNA. One possibility is to use fluorescently-labelled and hybridization-sensitive probes. In order to yield more infor...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/1746-4811-10-15
更新日期：2014-05-31 00:00:00
abstract：BACKGROUND:Effects of abiotic and biotic stresses on plant photosynthetic performance lead to fitness and yield decrease. The maximum quantum efficiency of photosystem II (F v/F m) is a parameter of chlorophyll fluorescence (ChlF) classically used to track changes in photosynthetic performance. Despite recent technical...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/s13007-015-0067-5
更新日期：2015-03-26 00:00:00
abstract：BACKGROUND:Simultaneous analysis of multiple functional-related phytohormones and their metabolites will improve our understanding of interactions among different hormones in the same biologic process. RESULTS:A method was developed for simultaneous quantification of multiple phytohormones, abscisic acid, indole-3-ace...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/1746-4811-8-2
更新日期：2012-01-15 00:00:00
abstract：Background:Deep rooting is one of the most promising plant traits for improving crop yield under water-limited conditions. Most root phenotyping methods are designed for laboratory-grown plants, typically measuring very young plants not grown in soil and not allowing full development of the root system. Results:This s...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/s13007-019-0533-6
更新日期：2019-12-09 00:00:00
abstract：BACKGROUND:Fluorescent proteins are extraordinary tools for biology studies due to their versatility; they are used extensively to improve comprehension of plant-microbe interactions. The viral infection process can easily be tracked and imaged in a plant with fluorescent protein-tagged viruses. In plants, fluorescent ...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/1746-4811-10-22
更新日期：2014-07-05 00:00:00
abstract：BACKGROUND:Transcription factors (TFs) coordinate precise gene expression patterns that give rise to distinct phenotypic outputs. The identification of genes and transcriptional networks regulated by a TF often requires stable transformation and expression changes in plant cells. However, the production of stable trans...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/s13007-016-0141-7
更新日期：2016-10-19 00:00:00
abstract：BACKGROUND:Establishing transcriptional regulatory networks that include protein-protein and protein-DNA interactions has become a key component to better understanding many fundamental biological processes. Although a variety of techniques are available to expose protein-protein and protein-DNA interactions, unequivoc...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/1746-4811-4-25
更新日期：2008-10-27 00:00:00
abstract：BACKGROUND:The elucidation of complex biological systems requires integration of multiple molecular parameters. Accordingly, high throughput methods like transcriptomics, proteomics, metabolomics and lipidomics have emerged to provide the tools for successful system-wide investigations. Unfortunately, optimized analysi...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/s13007-016-0146-2
更新日期：2016-11-10 00:00:00
abstract：BACKGROUND:Genetic studies on the molecular mechanisms of the regulation of root growth require the characterisation of a specific root phenotype to be linked with a certain genotype. Such studies using classical labour-intensive methods are severely hindered due to the technical limitations that are associated with th...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/s13007-016-0135-5
更新日期：2016-06-24 00:00:00
abstract：Background:The stomata of plants mainly regulate gas exchange and water dispersion between the interior and external environments of plants and play a major role in the plants' health. The existing methods of stomata segmentation and measurement are mostly for specialized plants. The purpose of this research is to deve...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/s13007-019-0453-5
更新日期：2019-07-03 00:00:00
abstract：BACKGROUND:Maize haploid breeding technology can be used to rapidly develop homozygous lines, significantly shorten the breeding cycle and improve breeding efficiency. Rapid and accurate sorting haploid kernels is a prerequisite for the large-scale application of this technology. At present, the automatic haploid sorti...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/s13007-020-00703-4
更新日期：2021-01-06 00:00:00
abstract：BACKGROUND:RNA interference results in specific gene silencing by small-interfering RNAs (siRNAs). Synthetic siRNAs provide a powerful tool for manipulating gene expression but high cost suggests that novel siRNA production methods are desirable. Strong evolutionary conservation of siRNA structure suggested that siRNAs...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/1746-4811-3-13
更新日期：2007-11-25 00:00:00
abstract：: Microarrays are routine tools for transcript profiling, and genomic tiling arrays such as the Arabidopsis AGRONOMICS1 arrays have been found to be highly suitable for such experiments because changes in genome annotation can be easily integrated at the data analysis level. In a transcript profiling experiment, RNA la...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/1746-4811-8-18
更新日期：2012-06-13 00:00:00
abstract：BACKGROUND:Cyst nematodes are biotrophs that form specialized feeding structures in the roots of host plants, which consist of a syncytial fusion of hypertrophied cells. The formation of syncytium is accompanied by profound transcriptional changes and active metabolism in infected tissues. The challenge in gene express...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/s13007-016-0123-9
更新日期：2016-04-26 00:00:00
abstract：UNLABELLED: BACKGROUND:The powdery mildew disease represents a valuable patho-system to study the interaction between plant hosts and obligate biotrophic fungal pathogens. Numerous discoveries have been made on the basis of the quantitative evaluation of plant-powdery mildew interactions, especially in the context of ...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/1746-4811-8-35
更新日期：2012-08-31 00:00:00
abstract：Background:Image processing techniques have been widely used in the analysis of leaf characteristics. Earlier techniques for processing digital RGB color images of plant leaves had several drawbacks, such as inadequate de-noising, and adopting normal-probability statistical estimation models which have few parameters a...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/s13007-020-0561-2
更新日期：2020-02-26 00:00:00
abstract：Background:Antirrhinum (snapdragon) species are models for genetic and evolutionary research but recalcitrant to genetic transformation, limiting use of transgenic methods for functional genomics. Transient gene expression from viral vectors and virus-induced gene silencing (VIGS) offer transformation-free alternatives...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/s13007-020-00683-5
更新日期：2020-10-27 00:00:00
abstract：Background:Many agronomic traits have been bred into modern wheat varieties, but wheat (Triticum aestivum L.) continues to be vulnerable to heat stress, with high night-time temperature (HNT) stress shown to have large negative impact on yield and quality. Global mean temperature during the day is consistently warming ...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/s13007-019-0424-x
更新日期：2019-04-24 00:00:00
abstract：Background:Non-contact resonant ultrasound spectroscopy (NC-RUS) has been proven as a reliable technique for the dynamic determination of leaf water status. It has been already tested in more than 50 plant species. In parallel, relative water content (RWC) is highly used in the ecophysiological field to describe the de...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/s13007-019-0511-z
更新日期：2019-11-07 00:00:00
abstract：Background:Chickpea (Cicer arietinum L.), an important legume crop is one of the major source of dietary protein. Developing an efficient and reproducible transformation method is imperative to expedite functional genomics studies in this crop. Here, we present an optimized and detailed procedure for Agrobacterium rhiz...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/s13007-018-0315-6
更新日期：2018-07-06 00:00:00
abstract：BACKGROUND:It is well known that preparation of biological (plant and animal) tissues for Scanning Electron Microscopy (SEM) by chemical fixation and critical point drying results in shrinkage of tissues, often by up to 20-30%, depending on the tissue type and fixation protocol used. We sought to identify a protocol th...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/1746-4811-9-36
更新日期：2013-10-02 00:00:00
abstract：Background:Plant root apex is the major part to direct the root growth and development by responding to various signals/cues from internal and soil environments. To study and understand root system biology particularly at a molecular and cellular level, an Arabidopsis T-DNA insertional enhancer trap line J3411 expressi...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/s13007-019-0393-0
更新日期：2019-01-31 00:00:00
abstract：Background:Plastome (plastid genome) sequences provide valuable information for understanding the phylogenetic relationships and evolutionary history of plants. Although the rapid development of high-throughput sequencing technology has led to an explosion of plastome sequences, annotation remains a significant bottlen...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/s13007-019-0435-7
更新日期：2019-05-21 00:00:00
abstract：:[This corrects the article DOI: 10.1186/s13007-019-0478-9.]. ...
journal_title：Plant methods
pub_type： 已发布勘误
doi：10.1186/s13007-019-0495-8
更新日期：2019-10-10 00:00:00
