Abstract：
Background:Open tissue culture technique could be simplified by using different bacteriostatic agents. There is a great difference in the bacteriostatic effects of different antimicrobial agents on various explants. However, there is no report about the effective bacteriostatic agent for open tissue culture of Acacia auriculiformis. Results:We carried out the bud induction trials under open conditions to screen out an effective antibacterial agent for open tissue culture of A. auriculiformis. The results showed that the suitable type and concentration of bacteriostatic agent was 0.2 g L-1 Chlorothalonil, and the suitable explant type was middle shoot section with leaves (the shoot section with third to fifth axillary bud). The treatment of 0.8 g L-1 Carbendazim for 3 min was the most suitable strategy for explants disinfection, and October was the best time for explants collection. The suitable bud induction medium was 1/8 MS + agar 7 g L-1 + Chlorothalonil 0.2 g L-1 + 6-BA 1.5 mg L-1, and the bud induction rate was 99.54%. Conclusions:Our results revealed that Chlorothalonil is an effective bacteriostatic agent for bud induction of A. auriculiformis under open condition. These results would be very helpful for further establishment of open tissue culture technology for A. auriculiformis.
journal_name
Plant Methodsjournal_title
Plant methodsauthors
Huang L,Wang H,Shahid MQ,Zeng Bdoi
10.1186/s13007-019-0390-3subject
Has Abstractpub_date
2019-01-25 00:00:00pages
5issn
1746-4811pii
390journal_volume
15pub_type
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