Expanding the bioluminescent reporter toolkit for plant science with NanoLUC.

abstract：: Microarrays are routine tools for transcript profiling, and genomic tiling arrays such as the Arabidopsis AGRONOMICS1 arrays have been found to be highly suitable for such experiments because changes in genome annotation can be easily integrated at the data analysis level. In a transcript profiling experiment, RNA la...

journal_title：Plant methods

authors： Müller M,Patrignani A,Rehrauer H,Gruissem W,Hennig L

更新日期：2012-06-13 00:00:00

abstract：Background:Plasmopara obducens is the biotrophic oomycete responsible for impatiens downy mildew, a destructive disease of Impatiens that causes high crop loss. Currently, there are no available methods for the microscopic detection of P. obducens from leaves of impatiens, which may be contributing to the spread of the...

journal_title：Plant methods

authors： Salgado-Salazar C,Bauchan GR,Wallace EC,Crouch JA

更新日期：2018-10-24 00:00:00

abstract：:Chloroplasts play a great role for sustained wellbeing of life on the planet. They have the power and raw materials that can be used as sophisticated biological factories. They are rich in energy as they have lots of pigment-protein complexes capable of collecting sunlight, in sugar produced by photosynthesis and in m...

journal_title：Plant methods

authors： Adem M,Beyene D,Feyissa T

更新日期：2017-04-19 00:00:00

abstract：:Terahertz technology is still an evolving research field that attracts scientists with very different backgrounds working on a wide range of subjects. In the past two decades, it has been demonstrated that terahertz technology can provide a non-invasive tool for measuring and monitoring the water content of leaves and...

journal_title：Plant methods

authors： Gente R,Koch M

更新日期：2015-03-06 00:00:00

abstract：BACKGROUND:Maize haploid breeding technology can be used to rapidly develop homozygous lines, significantly shorten the breeding cycle and improve breeding efficiency. Rapid and accurate sorting haploid kernels is a prerequisite for the large-scale application of this technology. At present, the automatic haploid sorti...

journal_title：Plant methods

authors： Qu Y,Liu Z,Zhang Y,Yang J,Li H

更新日期：2021-01-06 00:00:00

abstract：BACKGROUND:When conducting plant research, the measurement of photosynthetic pigments can provide basic information on the physiological status of a plant. High-pressure liquid chromatography (HPLC) is becoming widely used for this purpose because it provides an accurate determination of a variety of photosynthetic pig...

journal_title：Plant methods

authors： Hu X,Tanaka A,Tanaka R

更新日期：2013-06-19 00:00:00

abstract：BACKGROUND:There is considerable interest in rapid assays or screening systems for assigning gene function. However, analysis of gene function in the flowers of some species is restricted due to the difficulty of producing stably transformed transgenic plants. As a result, experimental approaches based on transient gen...

journal_title：Plant methods

authors： Shang Y,Schwinn KE,Bennett MJ,Hunter DA,Waugh TL,Pathirana NN,Brummell DA,Jameson PE,Davies KM

更新日期：2007-01-08 00:00:00

abstract：Background:Root is the principal part of plants to absorb water and nutrient, anchor the plant and affect yield and quality. Non-destructive detection of root traits is difficult to perform due to the hidden nature of the root. Therefore, improved methods to measure roots are necessary to support plant breeding, and op...

journal_title：Plant methods

authors： Lu W,Wang X,Wang F

更新日期：2019-03-23 00:00:00

abstract：BACKGROUND:Striga species are noxious root hemi-parasitic weeds that debilitate cereal production in sub-Saharan Africa (SSA). Control options for Striga are limited and developing Striga resistant crop germplasm is regarded as the best and most sustainable control measure. Efforts to improve germplasm for Striga resis...

journal_title：Plant methods

authors： Runo S,Macharia S,Alakonya A,Machuka J,Sinha N,Scholes J

更新日期：2012-06-21 00:00:00

abstract：Background:Nowadays, there is an increasing demand for fast and reliable plant biomolecular analyses. Conventional methods for the isolation of nucleic acids are time-consuming and require multiple and often non-automatable steps to remove cellular interferences, with consequence that sample preparation is the major bo...

journal_title：Plant methods

authors： Marengo A,Cagliero C,Sgorbini B,Anderson JL,Emaus MN,Bicchi C,Bertea CM,Rubiolo P

更新日期：2019-03-09 00:00:00

abstract：Background:Deep rooting is one of the most promising plant traits for improving crop yield under water-limited conditions. Most root phenotyping methods are designed for laboratory-grown plants, typically measuring very young plants not grown in soil and not allowing full development of the root system. Results:This s...

journal_title：Plant methods

authors： Chen S,Svane SF,Thorup-Kristensen K

更新日期：2019-12-09 00:00:00

abstract：BACKGROUND:The inability to genetically transform any fern species has been a major technical barrier to unlocking fern biology. Initial attempts to overcome this limitation were based on transient transformation approaches or achieved very low efficiencies. A highly efficient method of stable transformation was recent...

journal_title：Plant methods

authors： Plackett AR,Rabbinowitsch EH,Langdale JA

更新日期：2015-07-03 00:00:00

abstract：Background:Plant root apex is the major part to direct the root growth and development by responding to various signals/cues from internal and soil environments. To study and understand root system biology particularly at a molecular and cellular level, an Arabidopsis T-DNA insertional enhancer trap line J3411 expressi...

journal_title：Plant methods

authors： Zhang L,Qin LN,Zeng ZR,Wu CZ,Gong YY,Liu LH,Cao FQ

更新日期：2019-01-31 00:00:00

abstract：BACKGROUND:The tomato (Solanum lycopersicum L.) plant is both an economically important food crop and an ideal dicot model to investigate various physiological phenomena not possible in Arabidopsis thaliana. Due to the great diversity of tomato cultivars used by the research community, it is often difficult to reliably...

journal_title：Plant methods

authors： Carvalho RF,Campos ML,Pino LE,Crestana SL,Zsögön A,Lima JE,Benedito VA,Peres LE

更新日期：2011-06-29 00:00:00

abstract：Background:High throughput non-destructive phenotyping is emerging as a significant approach for phenotyping germplasm and breeding populations for the identification of superior donors, elite lines, and QTLs. Detection and counting of spikes, the grain bearing organs of wheat, is critical for phenomics of a large set ...

journal_title：Plant methods

authors： Misra T,Arora A,Marwaha S,Chinnusamy V,Rao AR,Jain R,Sahoo RN,Ray M,Kumar S,Raju D,Jha RR,Nigam A,Goel S

更新日期：2020-03-18 00:00:00

abstract：Background:Many agronomic traits have been bred into modern wheat varieties, but wheat (Triticum aestivum L.) continues to be vulnerable to heat stress, with high night-time temperature (HNT) stress shown to have large negative impact on yield and quality. Global mean temperature during the day is consistently warming ...

journal_title：Plant methods

authors： Hein NT,Wagner D,Bheemanahalli R,Šebela D,Bustamante C,Chiluwal A,Neilsen ML,Jagadish SVK

更新日期：2019-04-24 00:00:00

abstract：BACKGROUND:Brassinosteriods (BRs) are a group of important phytohormones that have major effects on plant growth and development. To fully elucidate the function of BRs, a sensitive BR assay is required. However, most of the previously reported methods are tedious and time-consuming due to multiple pretreatment steps. ...

journal_title：Plant methods

authors： Ding J,Wu JH,Liu JF,Yuan BF,Feng YQ

更新日期：2014-11-24 00:00:00

abstract：Background:Wheat is one of the most widely grown crop in temperate climates for food and animal feed. In order to meet the demands of the predicted population increase in an ever-changing climate, wheat production needs to dramatically increase. Spike and grain traits are critical determinants of final yield and grain ...

journal_title：Plant methods

authors： Hughes N,Askew K,Scotson CP,Williams K,Sauze C,Corke F,Doonan JH,Nibau C

更新日期：2017-11-01 00:00:00

abstract：Background:Open tissue culture technique could be simplified by using different bacteriostatic agents. There is a great difference in the bacteriostatic effects of different antimicrobial agents on various explants. However, there is no report about the effective bacteriostatic agent for open tissue culture of Acacia a...

journal_title：Plant methods

authors： Huang L,Wang H,Shahid MQ,Zeng B

更新日期：2019-01-25 00:00:00

abstract：BACKGROUND:Establishing transcriptional regulatory networks that include protein-protein and protein-DNA interactions has become a key component to better understanding many fundamental biological processes. Although a variety of techniques are available to expose protein-protein and protein-DNA interactions, unequivoc...

journal_title：Plant methods

authors： Xie Z,Grotewold E

更新日期：2008-10-27 00:00:00

abstract：:Over the last 10 years, high-density SNP arrays and DNA re-sequencing have illuminated the majority of the genotypic space for a number of organisms, including humans, maize, rice and Arabidopsis. For any researcher willing to define and score a phenotype across many individuals, Genome Wide Association Studies (GWAS)...

journal_title：Plant methods

authors： Korte A,Farlow A

更新日期：2013-07-22 00:00:00

abstract：BACKGROUND:Suppression subtractive hybridization is a popular technique for gene discovery from non-model organisms without an annotated genome sequence, such as cowpea (Vigna unguiculata (L.) Walp). We aimed to use this method to enrich for genes expressed during drought stress in a drought tolerant cowpea line. Howev...

journal_title：Plant methods

authors： Coetzer N,Gazendam I,Oelofse D,Berger DK

更新日期：2010-04-01 00:00:00

abstract：UNLABELLED: BACKGROUND:Phytohormones are the key metabolites participating in the regulation of multiple functions of plant organism. Among them, jasmonates, as well as abscisic and salicylic acids are responsible for triggering and modulating plant reactions targeted against pathogens and herbivores, as well as resis...

journal_title：Plant methods

authors： Balcke GU,Handrick V,Bergau N,Fichtner M,Henning A,Stellmach H,Tissier A,Hause B,Frolov A

更新日期：2012-11-22 00:00:00

abstract：Background:Chloroplasts are critical organelles that perceive and convey metabolic and stress signals to different cellular components, while remaining the seat of photosynthesis and a metabolic factory. The proteomes of intact leaves, chloroplasts, and suborganellar fractions of plastids have been evaluated in the mod...

journal_title：Plant methods

authors： Bhattacharya O,Ortiz I,Walling LL

更新日期：2020-09-24 00:00:00

abstract：BACKGROUND:It is well known that preparation of biological (plant and animal) tissues for Scanning Electron Microscopy (SEM) by chemical fixation and critical point drying results in shrinkage of tissues, often by up to 20-30%, depending on the tissue type and fixation protocol used. We sought to identify a protocol th...

journal_title：Plant methods

authors： Talbot MJ,White RG

更新日期：2013-10-02 00:00:00

abstract：BACKGROUND:We previously developed a high-throughput system called 'Ice-Cap' for growing Arabidopsis seedlings in a 96-well format and rapidly collecting tissue for subsequent DNA extraction and genotyping. While the originally described Ice-Cap method is an effective tool for high-throughput genotyping, one shortcomin...

journal_title：Plant methods

authors： Clark KA,Krysan PJ

更新日期：2007-06-12 00:00:00

abstract：BACKGROUND:Simultaneous analysis of multiple functional-related phytohormones and their metabolites will improve our understanding of interactions among different hormones in the same biologic process. RESULTS:A method was developed for simultaneous quantification of multiple phytohormones, abscisic acid, indole-3-ace...

journal_title：Plant methods

authors： Liu H,Li X,Xiao J,Wang S

更新日期：2012-01-15 00:00:00

abstract：Background:Plant roots release a variety of organic compounds into the soil which alter the physical, chemical and biological properties of the rhizosphere. Root exudates are technically challenging to measure in soil because roots are difficult to access and exudates can be bound by minerals or consumed by microorgani...

journal_title：Plant methods

authors： Kawasaki A,Okada S,Zhang C,Delhaize E,Mathesius U,Richardson AE,Watt M,Gilliham M,Ryan PR

更新日期：2018-12-20 00:00:00

abstract：Background:Strigolactones represent the most recently described group of plant hormones involved in many aspects of plant growth regulation. Simultaneously, root exuded strigolactones mediate rhizosphere signaling towards beneficial arbuscular mycorrhizal fungi, but also attract parasitic plants. The seed germination o...

journal_title：Plant methods

authors： Floková K,Shimels M,Andreo Jimenez B,Bardaro N,Strnad M,Novák O,Bouwmeester HJ

更新日期：2020-09-17 00:00:00

abstract：BACKGROUND:Kinome profiling aims at the parallel analysis of kinase activities in a cell. Novel developed arrays containing consensus substrates for kinases are used to assess those kinase activities. The arrays described in this paper were already used to determine kinase activities in mammalian systems, but since sub...

journal_title：Plant methods

authors： Ritsema T,Joore J,van Workum W,Pieterse CM

更新日期：2007-02-12 00:00:00