Abstract：
: Three terrestrial plants are known to perform C4 photosynthesis without the dual-cell system by partitioning two distinct types of chloroplasts in separate cytoplasmic compartments. We report herein a protocol for isolating the dimorphic chloroplasts from Bienertia sinuspersici. Hypo-osmotically lysed protoplasts under our defined conditions released intact compartments containing the central chloroplasts and intact vacuoles with adhering peripheral chloroplasts. Following Percoll step gradient purification both chloroplast preparations demonstrated high homogeneities as evaluated from the relative abundance of respective protein markers. This protocol will open novel research directions toward understanding the mechanism of single-cell C4 photosynthesis.
journal_name
Plant Methodsjournal_title
Plant methodsauthors
Lung SC,Yanagisawa M,Chuong SDdoi
10.1186/1746-4811-8-8subject
Has Abstractpub_date
2012-03-06 00:00:00pages
8issue
1issn
1746-4811pii
1746-4811-8-8journal_volume
8pub_type
杂志文章相关文献
Plant Methods文献大全abstract：BACKGROUND:Leaf rust (LR), caused by Puccinia triticina and is an important disease of wheat (Triticum aestivum L.). The most sustainable method for controlling rust diseases is deployment of cultivars incorporating adult plant resistance (APR). However, phenotyping breeding populations or germplasm collections for res...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/s13007-016-0117-7
更新日期：2016-03-02 00:00:00
abstract：Background:Wheat is one of the most widely grown crop in temperate climates for food and animal feed. In order to meet the demands of the predicted population increase in an ever-changing climate, wheat production needs to dramatically increase. Spike and grain traits are critical determinants of final yield and grain ...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/s13007-017-0229-8
更新日期：2017-11-01 00:00:00
abstract：BACKGROUND:Plant breeding and genetics demand fast, exact and reproducible phenotyping. Efficient statistical evaluation of phenotyping data requires standardised data storage ensuring long-term data availability while maintaining intellectual property rights. This is state of the art at phenomics centres, which, howev...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/s13007-015-0069-3
更新日期：2015-04-07 00:00:00
abstract：Background:Over the last years, several plant science labs have started to employ fluctuating growth light conditions to simulate natural light regimes more closely. Many plant mutants reveal quantifiable effects under fluctuating light despite being indistinguishable from wild-type plants under standard constant light...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/s13007-019-0546-1
更新日期：2019-12-23 00:00:00
abstract：Background:Deep rooting is one of the most promising plant traits for improving crop yield under water-limited conditions. Most root phenotyping methods are designed for laboratory-grown plants, typically measuring very young plants not grown in soil and not allowing full development of the root system. Results:This s...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/s13007-019-0533-6
更新日期：2019-12-09 00:00:00
abstract：Background:The cellular morphology of plant organs is strongly related to other physical properties such as shape, size, growth, mechanical properties or chemical composition. Cell morphology often vary depending on the type of tissue, or on the distance to a specific tissue. A common challenge in quantitative plant hi...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/s13007-020-00603-7
更新日期：2020-05-06 00:00:00
abstract：Motivation:In 2005, researchers from the French National Research Institute for Agriculture, Food and Environment (Institut national de recherche pour l'agriculture, l'alimentation et l'environnement, INRAE) started a collaboration with the French farmers' seed network Réseau Semences Paysannes (RSP) on bread wheat par...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/s13007-020-00640-2
更新日期：2020-07-23 00:00:00
abstract：Background:Plant parasitic weeds belonging to the genus Striga are a major threat for food production in Sub-Saharan Africa and Southeast Asia. The parasite's life cycle starts with the induction of seed germination by host plant-derived signals, followed by parasite attachment, infection, outgrowth, flowering, reprodu...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/s13007-020-00602-8
更新日期：2020-05-01 00:00:00
abstract：BACKGROUND:Simultaneous analysis of multiple functional-related phytohormones and their metabolites will improve our understanding of interactions among different hormones in the same biologic process. RESULTS:A method was developed for simultaneous quantification of multiple phytohormones, abscisic acid, indole-3-ace...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/1746-4811-8-2
更新日期：2012-01-15 00:00:00
abstract：:[This corrects the article DOI: 10.1186/s13007-017-0166-6.]. ...
journal_title：Plant methods
pub_type： 杂志文章,已发布勘误
doi：10.1186/s13007-017-0249-4
更新日期：2017-11-16 00:00:00
abstract：BACKGROUND:Protein phosphorylation is accepted as a major regulatory pathway in plants. More than 1000 protein kinases are predicted in the Arabidopsis proteome, however, only a few studies look systematically for in vivo protein phosphorylation sites. Owing to the low stoichiometry and low abundance of phosphorylated ...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/1746-4811-1-9
更新日期：2005-11-01 00:00:00
abstract：Background:Plastome (plastid genome) sequences provide valuable information for understanding the phylogenetic relationships and evolutionary history of plants. Although the rapid development of high-throughput sequencing technology has led to an explosion of plastome sequences, annotation remains a significant bottlen...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/s13007-019-0435-7
更新日期：2019-05-21 00:00:00
abstract：Background:Insect herbivory poses a major threat to maize. Benzoxazinoids are important anti-insect secondary metabolites in maize, whose biosynthetic pathway has been extensively studied. However, yet little is known about how benzoxazinoids are regulated in maize, partly due to lack of mutant resources and recalcitra...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/s13007-019-0529-2
更新日期：2019-11-28 00:00:00
abstract：:Over the last 10 years, high-density SNP arrays and DNA re-sequencing have illuminated the majority of the genotypic space for a number of organisms, including humans, maize, rice and Arabidopsis. For any researcher willing to define and score a phenotype across many individuals, Genome Wide Association Studies (GWAS)...
journal_title：Plant methods
pub_type： 杂志文章,评审
doi：10.1186/1746-4811-9-29
更新日期：2013-07-22 00:00:00
abstract：Background:Monitoring the timing of seedling emergence and early development via high-throughput phenotyping with computer vision is a challenging topic of high interest in plant science. While most studies focus on the measurements of leaf area index or detection of specific events such as emergence, little attention ...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/s13007-020-00647-9
更新日期：2020-07-30 00:00:00
abstract：Background:Chloroplasts are critical organelles that perceive and convey metabolic and stress signals to different cellular components, while remaining the seat of photosynthesis and a metabolic factory. The proteomes of intact leaves, chloroplasts, and suborganellar fractions of plastids have been evaluated in the mod...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/s13007-020-00667-5
更新日期：2020-09-24 00:00:00
abstract：Background:Many agronomic traits have been bred into modern wheat varieties, but wheat (Triticum aestivum L.) continues to be vulnerable to heat stress, with high night-time temperature (HNT) stress shown to have large negative impact on yield and quality. Global mean temperature during the day is consistently warming ...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/s13007-019-0424-x
更新日期：2019-04-24 00:00:00
abstract：: Microarrays are routine tools for transcript profiling, and genomic tiling arrays such as the Arabidopsis AGRONOMICS1 arrays have been found to be highly suitable for such experiments because changes in genome annotation can be easily integrated at the data analysis level. In a transcript profiling experiment, RNA la...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/1746-4811-8-18
更新日期：2012-06-13 00:00:00
abstract：:Modern agriculture has created a demand for plant biotechnology products that provide durable resistance to insect pests, tolerance of herbicide applications for weed control, and agronomic traits tailored for specific geographies. These transgenic trait products require a modular and sequential multigene stacking pla...
journal_title：Plant methods
pub_type： 杂志文章,评审
doi：10.1186/s13007-016-0111-0
更新日期：2016-02-01 00:00:00
abstract：:Plants are good sources of biologically active compounds with antimicrobial activity, including polypeptides. Antimicrobial peptides (AMPs) represent one of the main barriers of plant innate immunity to environmental stress factors and are attracting much research interest. There are some extraction methods for isolat...
journal_title：Plant methods
pub_type： 杂志文章,评审
doi：10.1186/s13007-020-00687-1
更新日期：2020-10-23 00:00:00
abstract：Background:In-field measurement of yield and growth rate in pasture species is imprecise and costly, limiting scientific and commercial application. Our study proposed a LiDAR-based mobile platform for non-invasive vegetative biomass and growth rate estimation in perennial ryegrass (Lolium perenne L.). This included de...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/s13007-019-0456-2
更新日期：2019-07-10 00:00:00
abstract：Background:Woody tropical plants contain high levels of complex organic compounds that inhibit the chemical procedures needed to extract RNA or DNA, thus compromising downstream applications such as RNA sequencing and analysis of gene expression. To overcome this issue, researchers must use extraction protocols using C...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/s13007-019-0447-3
更新日期：2019-06-05 00:00:00
abstract：BACKGROUND:Fluorescent proteins are extraordinary tools for biology studies due to their versatility; they are used extensively to improve comprehension of plant-microbe interactions. The viral infection process can easily be tracked and imaged in a plant with fluorescent protein-tagged viruses. In plants, fluorescent ...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/1746-4811-10-22
更新日期：2014-07-05 00:00:00
abstract：Background:Progress in high-throughput molecular methods accompanied by more complex experimental designs demands novel data visualisation solutions. To specifically answer the question which parts of the specifical biological system are responding in particular perturbation, integrative approach in which experimental ...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/s13007-018-0345-0
更新日期：2018-08-30 00:00:00
abstract：BACKGROUND:When conducting plant research, the measurement of photosynthetic pigments can provide basic information on the physiological status of a plant. High-pressure liquid chromatography (HPLC) is becoming widely used for this purpose because it provides an accurate determination of a variety of photosynthetic pig...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/1746-4811-9-19
更新日期：2013-06-19 00:00:00
abstract：Background:An effective and expeditious approach to assess plant nitrogen status is urgently needed in rice production and management as the conventional chemical methods are laborious and time-consuming. Results:Fourier transform infrared photoacoustic spectroscopy (FTIR-PAS) was used to record the spectra of rice le...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/s13007-019-0482-0
更新日期：2019-08-19 00:00:00
abstract：UNLABELLED: BACKGROUND:The powdery mildew disease represents a valuable patho-system to study the interaction between plant hosts and obligate biotrophic fungal pathogens. Numerous discoveries have been made on the basis of the quantitative evaluation of plant-powdery mildew interactions, especially in the context of ...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/1746-4811-8-35
更新日期：2012-08-31 00:00:00
abstract：BACKGROUND:Nectar reabsorption is a widely known phenomenon, related to the strategy of resource-recovery and also to maintain the nectar homeostasis at the nectary. The method currently performed to demonstrate nectar being reabsorbed involves the use of radioactive tracers applied to the nectary. Although this method...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/1746-4811-9-20
更新日期：2013-06-19 00:00:00
abstract：BACKGROUND:The data generated during a course of a biological experiment/study can be sometimes be massive and its management becomes quite critical for the success of the investigation undertaken. The accumulation and analysis of such large datasets often becomes tedious for biologists and lab technicians. Most of the...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/1746-4811-5-18
更新日期：2009-12-10 00:00:00
abstract：BACKGROUND:Brassinosteriods (BRs) are a group of important phytohormones that have major effects on plant growth and development. To fully elucidate the function of BRs, a sensitive BR assay is required. However, most of the previously reported methods are tedious and time-consuming due to multiple pretreatment steps. ...
journal_title：Plant methods
pub_type： 杂志文章
doi：10.1186/1746-4811-10-39
更新日期：2014-11-24 00:00:00
