Increased recovery and improved purity of PHA from recombinant Cupriavidus necator.

abstract：:Mammalian collagen has been widely used as a biomedical material. Nevertheless, there are still concerns about the variability between preparations, particularly with the possibility that the products may transmit animal-based diseases. Many groups have examined the possible application of bioengineered mammalian coll...

journal_title：Bioengineered

authors： Ramshaw JA,Werkmeister JA,Dumsday GJ

更新日期：2014-07-01 00:00:00

abstract：:In recent years there has been great progress with the implementation and utilization of Clustered Regularly Interspaced Palindromic Repeats (CRISPR) and CRISPR-associated protein (Cas) systems in the world of genetic engineering. Many forms of CRISPR-Cas9 have been developed as genome editing tools and techniques and...

journal_title：Bioengineered

authors： Perez Rojo F,Nyman RKM,Johnson AAT,Navarro MP,Ryan MH,Erskine W,Kaur P

更新日期：2018-01-01 00:00:00

abstract：:Vaccination is the most cost effective strategy for the control and prevention of the plethora of viral diseases affecting poultry production. The major challenge for poultry vaccination is the design of vaccines that will protect against multiple pathogens via a single protective dose, delivered by mass vaccination. ...

journal_title：Bioengineered

authors： Iqbal M

更新日期：2012-07-01 00:00:00

abstract：:Extraction of sugar is the rate-limiting step in converting unpretreated biomass into value-added products through microbial fermentation. Both anaerobic fungi and anaerobic bacteria have evolved to produce large multi-cellulase complexes referred to as cellulosomes, which are powerful machines for biomass deconstruct...

journal_title：Bioengineered

authors： Gilmore SP,Henske JK,O'Malley MA

更新日期：2015-01-01 00:00:00

abstract：:The anti-inflammatory cytokine interleukin-10 and its viral homologs were chosen as model proteins for the development of drug delivery systems based on probiotic carriers like E. coli Nissle 1917, E. coli G3/10, and Saccharomyces boulardii. Exterior cytokine secretion was achieved by a modified E. coli hemolysin tran...

journal_title：Bioengineered

authors： Pöhlmann C,Thomas M,Förster S,Brandt M,Hartmann M,Bleich A,Gunzer F

更新日期：2013-05-01 00:00:00

abstract：:Alkaline pectinase has important applications in the pretreatment of waste water from food processing and in both the fabric and paper industries. In this study, a 2-level factorial design was used to screen significant factors that affect the activity of alkaline pectinase, and the response surface methodology (RSM) ...

journal_title：Bioengineered

authors： Yu P,Zhang Y,Gu D

更新日期：2017-09-03 00:00:00

abstract：:Molybdoenzymes play essential functions in living organisms and, as a result, in various geochemical cycles. It is thus crucial to understand how these complex proteins become highly efficient enzymes able to perform a wide range of catalytic activities. It has been established that specific chaperones are involved du...

journal_title：Bioengineered

authors： Lemaire ON,Bouillet S,Méjean V,Iobbi-Nivol C,Genest O

更新日期：2017-03-04 00:00:00

abstract：:We have employed upstream open reading frames (uORFs) to systematically tune the translation levels of recombinant proteins. We present the design principles that guided the development of this technology and provide information that may help others in implementing synthetic uORFs for their own applications. We also r...

journal_title：Bioengineered

authors： Ferreira JP,Noderer WL,Diaz de Arce AJ,Wang CL

更新日期：2014-05-01 00:00:00

abstract：:Pancreatic adenocarcinoma (PAAD) ranks among the most lethal cancers worldwide with high mortality. A marked increase in the level of long non-coding RNA LINC00460 was reported in PAAD patients, in comparison with the healthy controls. However, the underlying mechanisms of the above phenomenon are not yet well underst...

journal_title：Bioengineered

authors： Cheng J,Lou Y,Jiang K

更新日期：2021-12-01 00:00:00

abstract：:The bioengineering tools have significant advantages through less time-consuming and utilized as a promising stage for the production of pharmaceutical bioproducts under the single platform. This review highlighted the advantages and current improvement in the plant, animal and microbial bioengineering tools and outli...

journal_title：Bioengineered

authors： Sarsaiya S,Shi J,Chen J

更新日期：2019-12-01 00:00:00

abstract：:As the second most common gynecologic malignant tumors with a high mortality rate, cervical cancer jeopardizes women's life worldwide. The low cure rate in cervical cancer patients is mainly attributed to the lack of effective therapies. One feasible novel strategy is to develop immune-based approaches such as adoptiv...

journal_title：Bioengineered

authors： Zheng Y,Yang Y,Wu S,Zhu Y,Tang X,Liu X

更新日期：2017-07-04 00:00:00

abstract：:We previously presented the cloning, heterologous expression, and characterization of a novel multidomain endoxylanase from Arthrobacter sp. GN16 isolated from the feces of Grus nigricollis. Molecular and biochemical characterization studies indicate that the glycoside hydrolase (GH) family 10 domain at the N-terminus...

journal_title：Bioengineered

authors： Zhou J,Liu Y,Shen J,Zhang R,Tang X,Li J,Wang Y,Huang Z

更新日期：2015-01-01 00:00:00

abstract：:We propose a mechanism of action for the betL* mutation which is based on DNA topology. Removing a single thymine residue from the betL σ(A) promoter's -10 and -35 spacer results in a 'twist'-mediated activation of transcription which accounts for the osmotolerance phenotype observed for strains expressing betL*. ...

journal_title：Bioengineered

authors： Keane SM,Culligan EP,Hoffmann RF,Gahan CG,Hill C,Snelling WJ,Sleator RD

更新日期：2016-04-02 00:00:00

abstract：:The influenza A virus is a critical public health problem that causes epidemics and pandemics, and occurs widely all over the world. Various vaccines against the virus have not provided a solution to the problem. Different approaches, particularly M2e peptide-based vaccines, are available for developing universal vacc...

journal_title：Bioengineered

authors： Kilinc YB,Akdeste ZM,Koc RC,Bagirova M,Allahverdiyev A

更新日期：2014-01-01 00:00:00

abstract：:This invited commentary covers the period 1997-2012 and has seen changes in terminology that progressed from "basic" and "applied" to "translational" research. In the context of Bioengineered, these changes map readily onto the processes of identifying microbial characteristics appropriate for specific applications, i...

journal_title：Bioengineered

authors： Dunne C

更新日期：2013-07-01 00:00:00

abstract：:Illicium verum (badiane or star anise), Crataegus oxyacantha ssp monogyna (hawthorn) and Allium cepa (onion), have traditionnally been used as medicinal plants in Algeria. This study showed that the outer layer of onion is rich in flavonols with contents of 103 ± 7.90 µg/g DW (red variety) and 17.3 ± 0.69 µg/gDW (whit...

journal_title：Bioengineered

authors： Benmalek Y,Yahia OA,Belkebir A,Fardeau ML

更新日期：2013-07-01 00:00:00

abstract：:A genetic recombinant Saccharomyces cerevisiae starter with high ethanol tolerance capacities was constructed. In this study, the gene of trehalose-6-phosphate synthase (encoded by tps1), which catalyzes the first step in trehalose synthesis, was cloned and overexpressed in S. cerevisiae. Moreover, the gene of neutral...

journal_title：Bioengineered

authors： Divate NR,Chen GH,Wang PM,Ou BR,Chung YC

更新日期：2016-11-01 00:00:00

abstract：:Bovine chymosin is an important milk-clotting agent used in the manufacturing of cheeses. Currently, the production of recombinant proteins by genetically modified organisms is widespread, leading to greatly reduced costs. Lactococcus (L.) lactis, the model lactic acid bacterium, was considered a good candidate for he...

journal_title：Bioengineered

authors： Luerce TD,Azevedo MS,LeBlanc JG,Azevedo V,Miyoshi A,Pontes DS

更新日期：2014-01-01 00:00:00

abstract：:Lon protease is conserved from bacteria to humans and regulates cellular processes by degrading different classes of proteins including antitoxins, transcriptional activators, unfolded proteins, and free ribosomal proteins. Since we found that Lon has several putative cyclic diguanylate (c-di-GMP) binding sites and si...

journal_title：Bioengineered

authors： Osbourne DO,Soo VW,Konieczny I,Wood TK

更新日期：2014-07-01 00:00:00

abstract：:Economically feasible production of second-generation biofuels requires efficient co-fermentation of pentose and hexose sugars in lignocellulosic hydrolysates under very harsh conditions. Baker's yeast is an excellent, traditionally used ethanol producer but is naturally not able to utilize pentoses. This is due to th...

journal_title：Bioengineered

authors： Oreb M,Dietz H,Farwick A,Boles E

更新日期：2012-11-01 00:00:00

abstract：:Engineered zinc-finger nucleases (ZFNs) are powerful tools for creating double-stranded-breaks (DSBs) in genomic DNA in a site-specific manner. These DSBs generated by ZFNs can be repaired by homology-directed repair or nonhomologous end joining, in which the latter can be exploited to generate insertion or deletion m...

journal_title：Bioengineered

authors： Haryadi R,Zhang P,Chan KF,Song Z

更新日期：2013-03-01 00:00:00

abstract：:Biodiesel, as one type of renewable energy, is an ideal substitute for petroleum-based diesel fuel and is usually made from triacylglycerides by transesterification with alcohols. Biodiesel production based on microbial fermentation aiming to establish more efficient, less-cost and sustainable biodiesel production str...

journal_title：Bioengineered

authors： Lin H,Wang Q,Shen Q,Zhan J,Zhao Y

更新日期：2013-09-01 00:00:00

abstract：:In this study, a total of six fungal samples were isolated from apple, strawberry and orange pulp. DNA sequence analysis was used as molecular identification method. ITS region was aligned in DNA sequence analysis, and an algorithm sequence similarity was done using BLAST (Basic Local Alignment Search Tool) program to...

journal_title：Bioengineered

authors： Asci F,Aydin B,Akkus GU,Unal A,Erdogmus SF,Korcan SE,Jahan I

更新日期：2020-12-01 00:00:00

abstract：:We report the molecular cloning, expression, and single-step homogeneous purification of RNA polymerase II subunit RPB5 from Saccharomyces cerevisiae. RPB5 is a 210 amino acid nuclear protein that functions as the fifth largest subunit of polymerase II and plays a central role in transcription. The gene that codes for...

journal_title：Bioengineered

authors： Chhetri G,Ghosh A,Chinta R,Akhtar S,Tripathi T

更新日期：2015-01-01 00:00:00

abstract：:Due to the limited self-renewal capacity of cardiomyocytes, the mammalian heart exhibits impaired regeneration and insufficient ability to restore heart function after injury. Cardiovascular tissue engineering is currently considered as a promising alternative therapy to restore the structure and function of the faili...

journal_title：Bioengineered

authors： Serpooshan V,Zhao M,Metzler SA,Wei K,Shah PB,Wang A,Mahmoudi M,Malkovskiy AV,Rajadas J,Butte MJ,Bernstein D,Ruiz-Lozano P

更新日期：2014-05-01 00:00:00

abstract：:This study is aimed to explore the roles of LINC00528 in myocardial infarction (MI) progression. Quantitative real-time PCR showed that the expression of LINC00528 and COX-2 was upregulated while miR-143-3p expression was down-regulated in post-MI cells. In function assays, LINC00528 suppression promoted post-MI cells...

journal_title：Bioengineered

authors： Liu K,Zhao D,Wang D

更新日期：2020-12-01 00:00:00

abstract：:The human chromogranin A-derived peptide CGA-N12, which is composed of 12 amino acid residues with the sequence ALQGAKERAHQQ, showed strong antifungal activity and the least hemolytic activity in previous studies. However, synthetic peptides are relatively expensive to produce. Recombinant expression of peptides in th...

journal_title：Bioengineered

authors： Li X,Fan Y,Lin Q,Luo J,Huang Y,Bao Y,Xu L

更新日期：2020-12-01 00:00:00

abstract：:Protein production through dedicated secretion systems might offer an potential alternative to the conventional cytoplasmical expression. The application of Type 1 secretion systems of Gram-negative bacteria, however, where often not successful in the past for a wide range of proteins. Recently, two studies using the ...

journal_title：Bioengineered

authors： Schwarz CK,Lenders MH,Smits SH,Schmitt L

更新日期：2012-09-01 00:00:00

abstract：:Production of psychrophilic enzymes in the commonly used mesophilic expression systems is hampered by low intrinsic stability of the recombinant enzymes at the optimal host growth temperatures. Unless strategies for low-temperature expression are advanced, research on psychrophilic enzymes may end up being biased towa...

journal_title：Bioengineered

authors： Bjerga GE,Lale R,Williamson AK

更新日期：2016-01-01 00:00:00

abstract：:We demonstrated previously that expression of Rhizomucor miehei lipase (RML) in Pichia pastoris could be significantly increased by addition of gene propeptide, optimized signal peptide codons and manipulation of gene dosage. In this study, effects of various strategies on the protein synthesis and secretion pathways ...

journal_title：Bioengineered

authors： Huang J,Wang Q,Bu W,Chen L,Yang Z,Zheng W,Li Y,Li J

更新日期：2019-12-01 00:00:00