Engineering Saccharomyces cerevisiae for improvement in ethanol tolerance by accumulation of trehalose.

abstract：:N-linked glycosylation is a primary source of heterogeneity associated with recombinant monoclonal antibodies and plays a key role in a myriad of drug properties associated with biological function. The glycosylation profile of recombinant monoclonal antibodies is influenced by an array of cell culture inputs which mu...

journal_title：Bioengineered

authors： Schilling M,Feng P,Sosic Z,Traviglia SL

更新日期：2020-12-01 00:00:00

abstract：:The plant-specific RING-domain finger proteins play important roles in plant development and stress responses. We recently identified and functionally characterized a stress-induced gene OsSRFP1 (Oryza sativa Stress-related RING Finger Protein 1) from rice. We showed evidences of the biotechnological potential of the ...

journal_title：Bioengineered

authors： Fang H,Meng Q,Zhang H,Huang J

更新日期：2016-01-01 00:00:00

abstract：:Kidney renal clear cell carcinoma (ccRCC) is a malignant tumor originating from renal tubular epithelium, lncRNAs can regulate the occurrence and development of EMT by targeting EMT transcription factors. We constructed a new survival signature based on EMT-related differentially expressed lncRNAs obtained from The Ca...

journal_title：Bioengineered

authors： Cheng YB,Li S,Zhang YP,Zhu ZH,Zhao N

更新日期：2021-01-31 00:00:00

abstract：:Alkaline pectinase has important applications in the pretreatment of waste water from food processing and in both the fabric and paper industries. In this study, a 2-level factorial design was used to screen significant factors that affect the activity of alkaline pectinase, and the response surface methodology (RSM) ...

journal_title：Bioengineered

authors： Yu P,Zhang Y,Gu D

更新日期：2017-09-03 00:00:00

abstract：:In contrast to conventional, molecular medicine that focuses on targeting specific pathways, stem cell therapy aims to perturb many related mechanisms in order to derive therapeutic benefit. This emerging modality is inherently complex due to the variety of cell types that can be used, delivery approaches that need to...

journal_title：Bioengineered

authors： Momeni A,Neelamegham S,Parashurama N

更新日期：2017-07-04 00:00:00

abstract：:For somatic gene therapy of hemophilia B, hepatocytes as the main cellular host for expression of hFIX are attractive targets. In gene therapy protocols, an efficient expression vector equipped with cis-regulatory elements such as introns is required. With this in mind, hFIX-expressing plasmids equipped with different...

journal_title：Bioengineered

authors： Azadbakhsh AS,Sam MR,Farokhi F

更新日期：2016-11-01 00:00:00

abstract：:Touch is produced by sensations that include approaching, sliding, pressing, and temperature. This concept has become a target of research in biotechnology, especially in the field of bionic biology. This study measured sliding and pressing with traditional tactile sensors in order to improve a machine operator's judg...

journal_title：Bioengineered

authors： Chen L,Di C,Chen X,Li Z,Luo J

更新日期：2017-01-02 00:00:00

abstract：:We report the molecular cloning, expression, and single-step homogeneous purification of RNA polymerase II subunit RPB5 from Saccharomyces cerevisiae. RPB5 is a 210 amino acid nuclear protein that functions as the fifth largest subunit of polymerase II and plays a central role in transcription. The gene that codes for...

journal_title：Bioengineered

authors： Chhetri G,Ghosh A,Chinta R,Akhtar S,Tripathi T

更新日期：2015-01-01 00:00:00

abstract：:The deep-sea bacterium strain FA13 was isolated from the sediment of the South Atlantic Ocean and identified as Bacillus circulans based on 16S ribosomal DNA sequence. Through liquid fermentation with five media, the cell-free supernatant fermented with ISP2 showed the highest inhibition activities against mycelial gr...

journal_title：Bioengineered

authors： Zhou Y,Wang J,Gao X,Wang K,Wang W,Wang Q,Yan P

更新日期：2019-12-01 00:00:00

abstract：:Microbial enzymes have been used in a large number of fields, such as chemical, agricultural and biopharmaceutical industries. The enzyme production rate and yield are the main factors to consider when choosing the appropriate expression system for the production of recombinant proteins. Recombinant enzymes have been ...

journal_title：Bioengineered

authors： Liu L,Yang H,Shin HD,Chen RR,Li J,Du G,Chen J

更新日期：2013-07-01 00:00:00

abstract：:As the second most common gynecologic malignant tumors with a high mortality rate, cervical cancer jeopardizes women's life worldwide. The low cure rate in cervical cancer patients is mainly attributed to the lack of effective therapies. One feasible novel strategy is to develop immune-based approaches such as adoptiv...

journal_title：Bioengineered

authors： Zheng Y,Yang Y,Wu S,Zhu Y,Tang X,Liu X

更新日期：2017-07-04 00:00:00

abstract：:The evolution of natural modular proteins and domain swapping by protein engineers have shown the disruptive potential of non-homologous recombination to create proteins with novel functions or traits. Bacteriophage endolysins, cellulosomes and polyketide synthases are 3 examples of natural modular proteins with each ...

journal_title：Bioengineered

authors： Maervoet VET,Briers Y

更新日期：2017-05-04 00:00:00

abstract：:Botulinum toxin type A can temporarily inhibit muscle contraction. Currently, physicians administer this toxin as a bio-drug in treatment of some muscle contraction disorders. TAT-BoNT/A(1-448) is a functional recombinant protein derived from botulinum toxin light chain. Unlike the full length botulinum toxin, TAT-BoN...

journal_title：Bioengineered

authors： Saffarian P,Peerayeh SN,Amani J,Ebrahimi F,Sedighianrad H,Halabian R,Imani Fooladi AA

更新日期：2016-11-01 00:00:00

abstract：:This invited commentary covers the period 1997-2012 and has seen changes in terminology that progressed from "basic" and "applied" to "translational" research. In the context of Bioengineered, these changes map readily onto the processes of identifying microbial characteristics appropriate for specific applications, i...

journal_title：Bioengineered

authors： Dunne C

更新日期：2013-07-01 00:00:00

abstract：:Tryptophan, an aromatic amino acid, has been widely used in food industry because it participates in the regulation of protein synthesis and metabolic network in vivo. In this study, we obtained a strain named TRP03 by enhancing the tryptophan synthesis pathway, which could accumulate tryptophan at approximately 35 g/...

journal_title：Bioengineered

authors： Du L,Zhang Z,Xu Q,Chen N

更新日期：2019-12-01 00:00:00

abstract：:The successful treatment of keloids is a great challenge in the plastic surgery field. Activating transcription factor 3 (ATF3) is discovered as an adaptive responsive gene, which plays a critical role in fibroblast activation. This study aimed to investigate the expression and biological role of ATF3 in the pathogene...

journal_title：Bioengineered

authors： Wang XM,Liu XM,Wang Y,Chen ZY

更新日期：2021-12-01 00:00:00

abstract：:The major drawback of chemical transformations for the production of 2, 5-furan dicarboxylic acid (FDCA) implies the usage of hazardous chemicals, high temperature and high pressure from nonrenewable resources. Alternate to chemical methods, biological methods are promising. Microbial FDCA production is improved throu...

journal_title：Bioengineered

authors： Rajesh RO,Godan TK,Sindhu R,Pandey A,Binod P

更新日期：2020-12-01 00:00:00

abstract：:The detecting mechanism of carbon nanotubes gas sensor based on multi-stable stochastic resonance (MSR) model was studied in this paper. A numerically stimulating model based on MSR was established. And gas-ionizing experiment by adding electronic white noise to induce 1.65 MHz periodic component in the carbon nanotub...

journal_title：Bioengineered

authors： Jingyi Z

更新日期：2015-01-01 00:00:00

abstract：:Due to the limited self-renewal capacity of cardiomyocytes, the mammalian heart exhibits impaired regeneration and insufficient ability to restore heart function after injury. Cardiovascular tissue engineering is currently considered as a promising alternative therapy to restore the structure and function of the faili...

journal_title：Bioengineered

authors： Serpooshan V,Zhao M,Metzler SA,Wei K,Shah PB,Wang A,Mahmoudi M,Malkovskiy AV,Rajadas J,Butte MJ,Bernstein D,Ruiz-Lozano P

更新日期：2014-05-01 00:00:00

abstract：:A simple procedure for recovering biodegradable polymer from bacterial cells has been developed using economical and environmentally friendly solvent or chemicals. Recombinant bacterium, Cupriavidus necator harboring pBBR1MCS-C2 plasmid polyhydroxyalkanoate (PHA) synthase gene was used for the production of copolymer ...

journal_title：Bioengineered

authors： Anis SN,Iqbal NM,Kumar S,Al-Ashraf A

更新日期：2013-03-01 00:00:00

abstract：:Increasing evidence has shown that the tumor microenvironment (TME) plays an important role in tumor occurrence and development and can also affect patient prognosis. In this study, we screened key prognostic genes in the breast cancer (BC) TME by analyzing the immune and stromal scores of tumor samples to detect diff...

journal_title：Bioengineered

authors： Ye Q,Han X,Wu Z

更新日期：2020-12-01 00:00:00

abstract：:Molybdoenzymes play essential functions in living organisms and, as a result, in various geochemical cycles. It is thus crucial to understand how these complex proteins become highly efficient enzymes able to perform a wide range of catalytic activities. It has been established that specific chaperones are involved du...

journal_title：Bioengineered

authors： Lemaire ON,Bouillet S,Méjean V,Iobbi-Nivol C,Genest O

更新日期：2017-03-04 00:00:00

abstract：:The objective is to explore the effect of testosterone on the proliferation and collagen synthesis of neonatal rat cardiac fibroblasts (CF) induced by Angiotensin II (Ang II) and the underlying mechanisms. Derived from neonatal rats, the CFs were divided into 4 groups: the control group, Ang II group, testosterone gro...

journal_title：Bioengineered

authors： Yang X,Wang Y,Yan S,Sun L,Yang G,Li Y,Yu C

更新日期：2017-01-02 00:00:00

abstract：:This experiment aimed to investigate the role of 24-epibrassinolide (EBL) against NaCl-induced salinity stress in Acacia gerrardii Benth. NaCl (200 mM) imparted deleterious effects on the growth and chlorophyll contents of A. gerrardii, but foliar application of EBL (1.0 mg/l; each plant received 2.5 ml) mitigated the...

journal_title：Bioengineered

authors： Abd Allah EF,Alqarawi AA,Hashem A,Wirth S,Egamberdieva D

更新日期：2018-01-01 00:00:00

abstract：:Circular RNA (circRNA) circ-LRP6 was recently proven to be a pivotal player in various human diseases. Nevertheless, its role in esophageal squamous cell cancer (ESCC) remains unknown. In the current study, we investigated the expression level, biological function and mechanism of circ-LRP6 in ESCC. Circ-LRP6 was sign...

journal_title：Bioengineered

authors： Wang J,Zhu W,Tao G,Wang W

更新日期：2020-12-01 00:00:00

abstract：:We previously presented the cloning, heterologous expression, and characterization of a novel multidomain endoxylanase from Arthrobacter sp. GN16 isolated from the feces of Grus nigricollis. Molecular and biochemical characterization studies indicate that the glycoside hydrolase (GH) family 10 domain at the N-terminus...

journal_title：Bioengineered

authors： Zhou J,Liu Y,Shen J,Zhang R,Tang X,Li J,Wang Y,Huang Z

更新日期：2015-01-01 00:00:00

abstract：:We propose a mechanism of action for the betL* mutation which is based on DNA topology. Removing a single thymine residue from the betL σ(A) promoter's -10 and -35 spacer results in a 'twist'-mediated activation of transcription which accounts for the osmotolerance phenotype observed for strains expressing betL*. ...

journal_title：Bioengineered

authors： Keane SM,Culligan EP,Hoffmann RF,Gahan CG,Hill C,Snelling WJ,Sleator RD

更新日期：2016-04-02 00:00:00

abstract：:Protein production through dedicated secretion systems might offer an potential alternative to the conventional cytoplasmical expression. The application of Type 1 secretion systems of Gram-negative bacteria, however, where often not successful in the past for a wide range of proteins. Recently, two studies using the ...

journal_title：Bioengineered

authors： Schwarz CK,Lenders MH,Smits SH,Schmitt L

更新日期：2012-09-01 00:00:00

abstract：:Ulcerative colitis (UC) is a type of refractory and recurrent inflammatory disorder that occurs in colon and rectum. Immune cell infiltration plays a critical role in UC progression; therefore, this study aims to explore potential biomarkers for UC and to analyze characteristics of immune cell infiltration based on th...

journal_title：Bioengineered

authors： Xue G,Hua L,Zhou N,Li J

更新日期：2021-12-01 00:00:00

abstract：:Bacterial L-asparaginase has been a universal component of therapies for childhood acute lymphoblastic leukemia since the 1970s. Two principal enzymes derived from Escherichia coli and Erwinia chrysanthemi are the only options clinically approved to date. We recently reported a study of recombinant L-asparaginase (Ans...

journal_title：Bioengineered

authors： Huerta-Saquero A,Evangelista-Martínez Z,Moreno-Enriquez A,Perez-Rueda E

更新日期：2013-01-01 00:00:00