abstract：:The successful treatment of keloids is a great challenge in the plastic surgery field. Activating transcription factor 3 (ATF3) is discovered as an adaptive responsive gene, which plays a critical role in fibroblast activation. This study aimed to investigate the expression and biological role of ATF3 in the pathogene...

journal_title：Bioengineered

authors： Wang XM,Liu XM,Wang Y,Chen ZY

更新日期：2021-12-01 00:00:00

abstract：:Tumor immunity is closely associated with the prognosis of tumors, including osteosarcoma (OS). The aim of the present study was to construct an immune-related prognostic index (PI) to predict the prognosis of OS. Herein, OS expression data were sourced from the Therapeutically Applicable Research to Generate Effectiv...

journal_title：Bioengineered

authors： Yin CD,Hou YL,Liu XR,He YS,Wang XP,Li CJ,Tan XH,Liu J

更新日期：2021-12-01 00:00:00

abstract：:The edible filamentous fungi are hot candidate for future supply of functional food and feed with e.g. protein, essential amino acids, and compounds with immunostimulant activity. L-carnitine that plays a crucial role in energy metabolism represents a functional compound normally produced by Zygomycetes filamentous fu...

journal_title：Bioengineered

authors： Rousta N,Ferreira JA,Taherzadeh MJ

更新日期：2021-12-01 00:00:00

abstract：:Ulcerative colitis (UC) is a type of refractory and recurrent inflammatory disorder that occurs in colon and rectum. Immune cell infiltration plays a critical role in UC progression; therefore, this study aims to explore potential biomarkers for UC and to analyze characteristics of immune cell infiltration based on th...

journal_title：Bioengineered

authors： Xue G,Hua L,Zhou N,Li J

更新日期：2021-12-01 00:00:00

abstract：:Pancreatic adenocarcinoma (PAAD) ranks among the most lethal cancers worldwide with high mortality. A marked increase in the level of long non-coding RNA LINC00460 was reported in PAAD patients, in comparison with the healthy controls. However, the underlying mechanisms of the above phenomenon are not yet well underst...

journal_title：Bioengineered

authors： Cheng J,Lou Y,Jiang K

更新日期：2021-12-01 00:00:00

abstract：:Kidney renal clear cell carcinoma (ccRCC) is a malignant tumor originating from renal tubular epithelium, lncRNAs can regulate the occurrence and development of EMT by targeting EMT transcription factors. We constructed a new survival signature based on EMT-related differentially expressed lncRNAs obtained from The Ca...

journal_title：Bioengineered

authors： Cheng YB,Li S,Zhang YP,Zhu ZH,Zhao N

更新日期：2021-01-31 00:00:00

abstract：:Circular RNA (circRNA) circ-LRP6 was recently proven to be a pivotal player in various human diseases. Nevertheless, its role in esophageal squamous cell cancer (ESCC) remains unknown. In the current study, we investigated the expression level, biological function and mechanism of circ-LRP6 in ESCC. Circ-LRP6 was sign...

journal_title：Bioengineered

authors： Wang J,Zhu W,Tao G,Wang W

更新日期：2020-12-01 00:00:00

abstract：:N-linked glycosylation is a primary source of heterogeneity associated with recombinant monoclonal antibodies and plays a key role in a myriad of drug properties associated with biological function. The glycosylation profile of recombinant monoclonal antibodies is influenced by an array of cell culture inputs which mu...

journal_title：Bioengineered

authors： Schilling M,Feng P,Sosic Z,Traviglia SL

更新日期：2020-12-01 00:00:00

abstract：:In this study, a total of six fungal samples were isolated from apple, strawberry and orange pulp. DNA sequence analysis was used as molecular identification method. ITS region was aligned in DNA sequence analysis, and an algorithm sequence similarity was done using BLAST (Basic Local Alignment Search Tool) program to...

journal_title：Bioengineered

authors： Asci F,Aydin B,Akkus GU,Unal A,Erdogmus SF,Korcan SE,Jahan I

更新日期：2020-12-01 00:00:00

abstract：:The human chromogranin A-derived peptide CGA-N12, which is composed of 12 amino acid residues with the sequence ALQGAKERAHQQ, showed strong antifungal activity and the least hemolytic activity in previous studies. However, synthetic peptides are relatively expensive to produce. Recombinant expression of peptides in th...

journal_title：Bioengineered

authors： Li X,Fan Y,Lin Q,Luo J,Huang Y,Bao Y,Xu L

更新日期：2020-12-01 00:00:00

abstract：NANNOCHLOROPSIS:sp. is a green alga that is widely used in the aquaculture industry as a feed in Malaysia, but genetic engineering studies of this alga are still underexplored even though there is a growing interest in microalgae genetic engineering for various industrial purposes. This study aims to investigate the ef...

journal_title：Bioengineered

authors： Zainal Abidin AA,Suntarajh M,Balia Yusof ZN

更新日期：2020-12-01 00:00:00

abstract：:Increasing evidence has shown that the tumor microenvironment (TME) plays an important role in tumor occurrence and development and can also affect patient prognosis. In this study, we screened key prognostic genes in the breast cancer (BC) TME by analyzing the immune and stromal scores of tumor samples to detect diff...

journal_title：Bioengineered

authors： Ye Q,Han X,Wu Z

更新日期：2020-12-01 00:00:00

abstract：:Rhizoremediation is one of the most accepted, cost-effective bioremediation techniques focusing on the application of rhizospheric microorganisms in combination with plants for the remediation of organic and inorganic pollutants from the contaminated sites. This work focuses on isolation and identification of metal re...

journal_title：Bioengineered

authors： Yaashikaa PR,Senthil Kumar P,Varjani S,Saravanan A

更新日期：2020-12-01 00:00:00

abstract：:By-products from the food sector with a high load of organic matter present both a waste-handling problem related to expenses and to the environment, yet also an opportunity. This study aims to increase the value of stale bread and brewers spent grain (BSG) by re-introducing these residues to the food production chain...

journal_title：Bioengineered

authors： Gmoser R,Fristedt R,Larsson K,Undeland I,Taherzadeh MJ,Lennartsson PR

更新日期：2020-12-01 00:00:00

abstract：:In this study, the conditions of extraction of loquat flowers polyphenolics were optimized through response surface methodology (RSM). Proper extraction conditions were: solid to liquid ratio 1 g per 50 mL and ethanol concentration 50% at 61°C for 9 min. Furthermore, the antioxidant and anti-polyphenol oxidase (PPO) a...

journal_title：Bioengineered

authors： Huang GL,Ma JJ,Sui SY,Wang YN

更新日期：2020-12-01 00:00:00

abstract：:The major drawback of chemical transformations for the production of 2, 5-furan dicarboxylic acid (FDCA) implies the usage of hazardous chemicals, high temperature and high pressure from nonrenewable resources. Alternate to chemical methods, biological methods are promising. Microbial FDCA production is improved throu...

journal_title：Bioengineered

authors： Rajesh RO,Godan TK,Sindhu R,Pandey A,Binod P

更新日期：2020-12-01 00:00:00

abstract：:Cyanobacteria are Gram-negative photoautotrophic prokaryotes and have shown great importance to the Earth's ecology. Based on their capability in oxygenic photosynthesis and genetic merits, they can be engineered as microbial chassis for direct conversion of carbon dioxide to value-added biofuels and chemicals. In the...

journal_title：Bioengineered

authors： Wang F,Gao Y,Yang G

更新日期：2020-12-01 00:00:00

abstract：:This study is aimed to explore the roles of LINC00528 in myocardial infarction (MI) progression. Quantitative real-time PCR showed that the expression of LINC00528 and COX-2 was upregulated while miR-143-3p expression was down-regulated in post-MI cells. In function assays, LINC00528 suppression promoted post-MI cells...

journal_title：Bioengineered

authors： Liu K,Zhao D,Wang D

更新日期：2020-12-01 00:00:00

abstract：:Endophytic fungi and bacteria are the most ubiquitous and representative commensal members that have been studied so far in various higher plants. Within colonization and interaction with their host plants, endophytic microbiota are reportedly to modulate not only the host's growth but also holobiont resilience to abi...

journal_title：Bioengineered

authors： Xie H,Feng X,Wang M,Wang Y,Kumar Awasthi M,Xu P

更新日期：2020-12-01 00:00:00

abstract：:Downregulation of lncRNA H19 (H19) expression is associated with an unfavorable prognosis in some cancers. However, little was known as to whether there was an association between H19 and minimally invasive follicular thyroid carcinoma (MI-FTC). In our study, we used quantitative real-time polymerase chain reaction (q...

journal_title：Bioengineered

authors： Dai Y,Miao Y,Zhu Q,Gao M,Hao F

更新日期：2019-12-01 00:00:00

abstract：:This study aimed to detect serum miR-203 expression levels in AML and explore its potential clinical significance. Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) was performed to measure the serum miR-203 levels in 134 patients with AML and 70 healthy controls. The results demonstrated that ser...

journal_title：Bioengineered

authors： Guo Y

更新日期：2019-12-01 00:00:00

abstract：:MiR-25 is a well-documented oncogenic miRNA implicated in esophageal squamous cell carcinoma (ESCC) development, progression and metastasis. However, whether and how miR-25 is involved in the development and metastasis of ESCC remain un-addressed. By using qRT-PCR analysis to compare levels of miR-25 in ESCC tissues w...

journal_title：Bioengineered

authors： Liu B,Li X,Li C,Xu R,Sun X

更新日期：2019-12-01 00:00:00

abstract：:The deep-sea bacterium strain FA13 was isolated from the sediment of the South Atlantic Ocean and identified as Bacillus circulans based on 16S ribosomal DNA sequence. Through liquid fermentation with five media, the cell-free supernatant fermented with ISP2 showed the highest inhibition activities against mycelial gr...

journal_title：Bioengineered

authors： Zhou Y,Wang J,Gao X,Wang K,Wang W,Wang Q,Yan P

更新日期：2019-12-01 00:00:00

abstract：:Stromal interaction molecule 1 (STIM1) is a calcium-sensing protein localized in the membrane of the endoplasmic reticulum. The expression of STIM1 has been shown to be closely associated with cell proliferation. The aim of the present study was to investigate the role of STIM1 in the regulation of cancer progression ...

journal_title：Bioengineered

authors： Ge C,Zeng B,Li R,Li Z,Fu Q,Wang W,Wang Z,Dong S,Lai Z,Wang Y,Xue Y,Guo J,Di T,Song X

更新日期：2019-12-01 00:00:00

abstract：:We demonstrated previously that expression of Rhizomucor miehei lipase (RML) in Pichia pastoris could be significantly increased by addition of gene propeptide, optimized signal peptide codons and manipulation of gene dosage. In this study, effects of various strategies on the protein synthesis and secretion pathways ...

journal_title：Bioengineered

authors： Huang J,Wang Q,Bu W,Chen L,Yang Z,Zheng W,Li Y,Li J

更新日期：2019-12-01 00:00:00

abstract：:Epstein-Barr virus (EBV) is an oncogenic virus that infects more than 90% of the world's population. The proteins and miRNAs encoded by EBV are involved in multiple human malignancies. Recently R-resistance RNA-seq demonstrated that EBV-encoded circular RNAs. The current research aims to explore their functions in EBV...

journal_title：Bioengineered

authors： Qiao Y,Zhao X,Liu J,Yang W

更新日期：2019-12-01 00:00:00

abstract：:Tryptophan, an aromatic amino acid, has been widely used in food industry because it participates in the regulation of protein synthesis and metabolic network in vivo. In this study, we obtained a strain named TRP03 by enhancing the tryptophan synthesis pathway, which could accumulate tryptophan at approximately 35 g/...

journal_title：Bioengineered

authors： Du L,Zhang Z,Xu Q,Chen N

更新日期：2019-12-01 00:00:00

abstract：:The bioengineering tools have significant advantages through less time-consuming and utilized as a promising stage for the production of pharmaceutical bioproducts under the single platform. This review highlighted the advantages and current improvement in the plant, animal and microbial bioengineering tools and outli...

journal_title：Bioengineered

authors： Sarsaiya S,Shi J,Chen J

更新日期：2019-12-01 00:00:00

abstract：:Bacterial CotA-laccases exhibit higher activity in alkaline pH and salt concentration conditions compared to laccases from white-rot fungi. They are considered as green catalysts in decolorizing of industrial dyes. However, CotA-laccases are limited due to the low yield and catalytic efficiency as the spore-bound natu...

journal_title：Bioengineered

authors： Ouyang F,Zhao M

更新日期：2019-12-01 00:00:00

abstract：:This experiment aimed to investigate the role of 24-epibrassinolide (EBL) against NaCl-induced salinity stress in Acacia gerrardii Benth. NaCl (200 mM) imparted deleterious effects on the growth and chlorophyll contents of A. gerrardii, but foliar application of EBL (1.0 mg/l; each plant received 2.5 ml) mitigated the...

journal_title：Bioengineered

authors： Abd Allah EF,Alqarawi AA,Hashem A,Wirth S,Egamberdieva D

更新日期：2018-01-01 00:00:00

abstract：:In recent years there has been great progress with the implementation and utilization of Clustered Regularly Interspaced Palindromic Repeats (CRISPR) and CRISPR-associated protein (Cas) systems in the world of genetic engineering. Many forms of CRISPR-Cas9 have been developed as genome editing tools and techniques and...

journal_title：Bioengineered

authors： Perez Rojo F,Nyman RKM,Johnson AAT,Navarro MP,Ryan MH,Erskine W,Kaur P

更新日期：2018-01-01 00:00:00

abstract：:Alkaline pectinase has important applications in the pretreatment of waste water from food processing and in both the fabric and paper industries. In this study, a 2-level factorial design was used to screen significant factors that affect the activity of alkaline pectinase, and the response surface methodology (RSM) ...

journal_title：Bioengineered

authors： Yu P,Zhang Y,Gu D

更新日期：2017-09-03 00:00:00

abstract：:The main treatment option for Hemophilia A/B patients involves the administration of recombinant coagulation factors on-demand or in a prophylactic approach. Despite the safety and efficacy of this replacement therapy, the development of antibodies against the coagulation factor infused, which neutralize the procoagul...

journal_title：Bioengineered

authors： Swiech K,Picanço-Castro V,Covas DT

更新日期：2017-09-03 00:00:00

abstract：:As the second most common gynecologic malignant tumors with a high mortality rate, cervical cancer jeopardizes women's life worldwide. The low cure rate in cervical cancer patients is mainly attributed to the lack of effective therapies. One feasible novel strategy is to develop immune-based approaches such as adoptiv...

journal_title：Bioengineered

authors： Zheng Y,Yang Y,Wu S,Zhu Y,Tang X,Liu X

更新日期：2017-07-04 00:00:00

abstract：:In contrast to conventional, molecular medicine that focuses on targeting specific pathways, stem cell therapy aims to perturb many related mechanisms in order to derive therapeutic benefit. This emerging modality is inherently complex due to the variety of cell types that can be used, delivery approaches that need to...

journal_title：Bioengineered

authors： Momeni A,Neelamegham S,Parashurama N

更新日期：2017-07-04 00:00:00

abstract：:Efficient assembly of multiple DNA fragments is a pivotal technology for synthetic biology. A scarless and sequence-independent DNA assembly method (DATEL) using thermal exonucleases has been developed recently. Here, we present a simplified DATEL (sDATEL) for efficient assembly of unphosphorylated DNA fragments with ...

journal_title：Bioengineered

authors： Ding W,Weng H,Jin P,Du G,Chen J,Kang Z

更新日期：2017-05-04 00:00:00

abstract：:The evolution of natural modular proteins and domain swapping by protein engineers have shown the disruptive potential of non-homologous recombination to create proteins with novel functions or traits. Bacteriophage endolysins, cellulosomes and polyketide synthases are 3 examples of natural modular proteins with each ...

journal_title：Bioengineered

authors： Maervoet VET,Briers Y

更新日期：2017-05-04 00:00:00

abstract：:Surface plasmon resonance (SPR) sensing is recently emerging as a valuable technique for measuring the binding constants, association and dissociation rate constants, and stoichimetry for a binding interaction kinetics in a number of emerging biological areas. This technique can be applied to the study of immune syste...

journal_title：Bioengineered

authors： Jahanshahi P,Wei Q,Jie Z,Ghomeishi M,Sekaran SD,Mahamd Adikan FR

更新日期：2017-05-04 00:00:00

abstract：:Molybdoenzymes play essential functions in living organisms and, as a result, in various geochemical cycles. It is thus crucial to understand how these complex proteins become highly efficient enzymes able to perform a wide range of catalytic activities. It has been established that specific chaperones are involved du...

journal_title：Bioengineered

authors： Lemaire ON,Bouillet S,Méjean V,Iobbi-Nivol C,Genest O

更新日期：2017-03-04 00:00:00

abstract：:Atherosclerosis enables to alter not only the microstructural but also the physical properties of the arterial walls by plaque forming. Few studies so far have been conducted to calculate the isotropic or anisotropic mechanical properties of the healthy and atherosclerotic human coronary arteries. To date there is a p...

journal_title：Bioengineered

authors： Karimi A,Rahmati SM,Sera T,Kudo S,Navidbakhsh M

更新日期：2017-03-04 00:00:00