From biocontrol to cancer, probiotics and beyond.

Abstract:

:This invited commentary covers the period 1997-2012 and has seen changes in terminology that progressed from "basic" and "applied" to "translational" research. In the context of Bioengineered, these changes map readily onto the processes of identifying microbial characteristics appropriate for specific applications, isolation of suitable cultures, strain or genome manipulation and exploitation of these or their metabolomes across a range of settings.   To a great degree, this commentary and my career reflect an engagement with molecular microbiology and the trialling of bacteria and derived constructs in applications ranging from intensive-scale crop protection to amelioration of gastrointestinal disease. This engagement began with laboratory and field evaluations of biocontrol, specifically use of pseudomonads effective against nematode and fungal plant pathogens, characterization of mechanisms mediating beneficial effects of probiotic lactobacilli and bifidobacteria and assessment of functional foods in multinational clinical trials relating to inflammatory bowel disease. Subsequent work focused on (1) intellectual property (IP)-based medical devices for localized delivery of systemically toxic and gene cancer therapies; (2) growth of the science base supporting expansion of a multinational business including company acquisitions; (3) complementing existing inter-institutional research capabilities through development of a national industry-led collaboration; and, most recently, (4) strategic research programs at Ireland's newest medical school. My activities as outlined above parallel two distinct aspects of translational research: (1) involvement in knowledge-driven (commercial and research) organizations that brought together necessary resources and infrastructure and (2) availability of scale research funding from European Framework and Irish national programs.

journal_name

Bioengineered

journal_title

Bioengineered

authors

Dunne C

doi

10.4161/bioe.23251

subject

Has Abstract

pub_date

2013-07-01 00:00:00

pages

185-90

issue

4

eissn

2165-5979

issn

2165-5987

pii

23251

journal_volume

4

pub_type

杂志文章
  • Effect of testosterone on the proliferation and collagen synthesis of cardiac fibroblasts induced by angiotensin II in neonatal rat.

    abstract::The objective is to explore the effect of testosterone on the proliferation and collagen synthesis of neonatal rat cardiac fibroblasts (CF) induced by Angiotensin II (Ang II) and the underlying mechanisms. Derived from neonatal rats, the CFs were divided into 4 groups: the control group, Ang II group, testosterone gro...

    journal_title:Bioengineered

    pub_type: 杂志文章

    doi:10.1080/21655979.2016.1227141

    authors: Yang X,Wang Y,Yan S,Sun L,Yang G,Li Y,Yu C

    更新日期:2017-01-02 00:00:00

  • Expression and purification of recombinant TAT-BoNT/A(1-448) under denaturing and native conditions.

    abstract::Botulinum toxin type A can temporarily inhibit muscle contraction. Currently, physicians administer this toxin as a bio-drug in treatment of some muscle contraction disorders. TAT-BoNT/A(1-448) is a functional recombinant protein derived from botulinum toxin light chain. Unlike the full length botulinum toxin, TAT-BoN...

    journal_title:Bioengineered

    pub_type: 杂志文章

    doi:10.1080/21655979.2016.1201252

    authors: Saffarian P,Peerayeh SN,Amani J,Ebrahimi F,Sedighianrad H,Halabian R,Imani Fooladi AA

    更新日期:2016-11-01 00:00:00

  • Friction properties of biological functional materials: PVDF membranes.

    abstract::Touch is produced by sensations that include approaching, sliding, pressing, and temperature. This concept has become a target of research in biotechnology, especially in the field of bionic biology. This study measured sliding and pressing with traditional tactile sensors in order to improve a machine operator's judg...

    journal_title:Bioengineered

    pub_type: 杂志文章

    doi:10.1080/21655979.2016.1227612

    authors: Chen L,Di C,Chen X,Li Z,Luo J

    更新日期:2017-01-02 00:00:00

  • Cell suspension concentration monitoring by using a miniaturized serial high frequency SAWR sensor.

    abstract::In this paper, a miniaturized cell suspension concentration monitoring method was investigated. The sensing unit was a carbon screen-printed electrode (CSPE) in serial with a 433MHz vacuum-packaged surface acoustic wave resonator (SAWR). SAWR provided a stable and high operating frequency, which helps to keep the stab...

    journal_title:Bioengineered

    pub_type: 杂志文章

    doi:10.1080/21655979.2015.1119342

    authors: Li J,Feng H,Fang Y

    更新日期:2015-01-01 00:00:00

  • Recombinant Lactococcus lactis fails to secrete bovine chymosine.

    abstract::Bovine chymosin is an important milk-clotting agent used in the manufacturing of cheeses. Currently, the production of recombinant proteins by genetically modified organisms is widespread, leading to greatly reduced costs. Lactococcus (L.) lactis, the model lactic acid bacterium, was considered a good candidate for he...

    journal_title:Bioengineered

    pub_type: 杂志文章

    doi:10.4161/bioe.36327

    authors: Luerce TD,Azevedo MS,LeBlanc JG,Azevedo V,Miyoshi A,Pontes DS

    更新日期:2014-01-01 00:00:00

  • Engineering Saccharomyces cerevisiae for improvement in ethanol tolerance by accumulation of trehalose.

    abstract::A genetic recombinant Saccharomyces cerevisiae starter with high ethanol tolerance capacities was constructed. In this study, the gene of trehalose-6-phosphate synthase (encoded by tps1), which catalyzes the first step in trehalose synthesis, was cloned and overexpressed in S. cerevisiae. Moreover, the gene of neutral...

    journal_title:Bioengineered

    pub_type: 杂志文章

    doi:10.1080/21655979.2016.1207019

    authors: Divate NR,Chen GH,Wang PM,Ou BR,Chung YC

    更新日期:2016-11-01 00:00:00

  • Different construction strategies affected on the physiology of Pichia pastoris strains highly expressed lipase by transcriptional analysis of key genes.

    abstract::We demonstrated previously that expression of Rhizomucor miehei lipase (RML) in Pichia pastoris could be significantly increased by addition of gene propeptide, optimized signal peptide codons and manipulation of gene dosage. In this study, effects of various strategies on the protein synthesis and secretion pathways ...

    journal_title:Bioengineered

    pub_type: 杂志文章

    doi:10.1080/21655979.2019.1614422

    authors: Huang J,Wang Q,Bu W,Chen L,Yang Z,Zheng W,Li Y,Li J

    更新日期:2019-12-01 00:00:00

  • Clinical significance of serum MicroRNA-203 in patients with acute myeloid leukemia.

    abstract::This study aimed to detect serum miR-203 expression levels in AML and explore its potential clinical significance. Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) was performed to measure the serum miR-203 levels in 134 patients with AML and 70 healthy controls. The results demonstrated that ser...

    journal_title:Bioengineered

    pub_type: 杂志文章,收录出版

    doi:10.1080/21655979.2019.1652490

    authors: Guo Y

    更新日期:2019-12-01 00:00:00

  • miR-25 mediates metastasis and epithelial-mesenchymal-transition in human esophageal squamous cell carcinoma via regulation of E-cadherin signaling.

    abstract::MiR-25 is a well-documented oncogenic miRNA implicated in esophageal squamous cell carcinoma (ESCC) development, progression and metastasis. However, whether and how miR-25 is involved in the development and metastasis of ESCC remain un-addressed. By using qRT-PCR analysis to compare levels of miR-25 in ESCC tissues w...

    journal_title:Bioengineered

    pub_type: 杂志文章

    doi:10.1080/21655979.2019.1687391

    authors: Liu B,Li X,Li C,Xu R,Sun X

    更新日期:2019-12-01 00:00:00

  • Production of L-carnitine-enriched edible filamentous fungal biomass through submerged cultivation.

    abstract::The edible filamentous fungi are hot candidate for future supply of functional food and feed with e.g. protein, essential amino acids, and compounds with immunostimulant activity. L-carnitine that plays a crucial role in energy metabolism represents a functional compound normally produced by Zygomycetes filamentous fu...

    journal_title:Bioengineered

    pub_type: 杂志文章

    doi:10.1080/21655979.2020.1863618

    authors: Rousta N,Ferreira JA,Taherzadeh MJ

    更新日期:2021-12-01 00:00:00

  • Bioengineering for Organ Transplantation: Progress and Challenges.

    abstract::Organ transplantation can offer a curative option for patients with end stage organ failure. Unfortunately the treatment is severely limited by the availability of donor organs. Organ bioengineering could provide a solution to the worldwide critical organ shortage. The majority of protocols to date have employed the u...

    journal_title:Bioengineered

    pub_type: 杂志文章,评审

    doi:10.1080/21655979.2015.1081320

    authors: Welman T,Michel S,Segaren N,Shanmugarajah K

    更新日期:2015-01-01 00:00:00

  • Expression of long non-coding RNA H19 predicts distant metastasis in minimally invasive follicular thyroid carcinoma.

    abstract::Downregulation of lncRNA H19 (H19) expression is associated with an unfavorable prognosis in some cancers. However, little was known as to whether there was an association between H19 and minimally invasive follicular thyroid carcinoma (MI-FTC). In our study, we used quantitative real-time polymerase chain reaction (q...

    journal_title:Bioengineered

    pub_type: 杂志文章

    doi:10.1080/21655979.2019.1658489

    authors: Dai Y,Miao Y,Zhu Q,Gao M,Hao F

    更新日期:2019-12-01 00:00:00

  • Combining MPDL3280A with adoptive cell immunotherapy exerts better antitumor effects against cervical cancer.

    abstract::As the second most common gynecologic malignant tumors with a high mortality rate, cervical cancer jeopardizes women's life worldwide. The low cure rate in cervical cancer patients is mainly attributed to the lack of effective therapies. One feasible novel strategy is to develop immune-based approaches such as adoptiv...

    journal_title:Bioengineered

    pub_type: 杂志文章

    doi:10.1080/21655979.2016.1230573

    authors: Zheng Y,Yang Y,Wu S,Zhu Y,Tang X,Liu X

    更新日期:2017-07-04 00:00:00

  • Study of detecting mechanism of carbon nanotubes gas sensor based on multi-stable stochastic resonance model.

    abstract::The detecting mechanism of carbon nanotubes gas sensor based on multi-stable stochastic resonance (MSR) model was studied in this paper. A numerically stimulating model based on MSR was established. And gas-ionizing experiment by adding electronic white noise to induce 1.65 MHz periodic component in the carbon nanotub...

    journal_title:Bioengineered

    pub_type: 杂志文章

    doi:10.1080/21655979.2015.1058453

    authors: Jingyi Z

    更新日期:2015-01-01 00:00:00

  • Use of bio-mimetic three-dimensional technology in therapeutics for heart disease.

    abstract::Due to the limited self-renewal capacity of cardiomyocytes, the mammalian heart exhibits impaired regeneration and insufficient ability to restore heart function after injury. Cardiovascular tissue engineering is currently considered as a promising alternative therapy to restore the structure and function of the faili...

    journal_title:Bioengineered

    pub_type: 杂志文章

    doi:10.4161/bioe.27751

    authors: Serpooshan V,Zhao M,Metzler SA,Wei K,Shah PB,Wang A,Mahmoudi M,Malkovskiy AV,Rajadas J,Butte MJ,Bernstein D,Ruiz-Lozano P

    更新日期:2014-05-01 00:00:00

  • CRISPR-Cas systems: ushering in the new genome editing era.

    abstract::In recent years there has been great progress with the implementation and utilization of Clustered Regularly Interspaced Palindromic Repeats (CRISPR) and CRISPR-associated protein (Cas) systems in the world of genetic engineering. Many forms of CRISPR-Cas9 have been developed as genome editing tools and techniques and...

    journal_title:Bioengineered

    pub_type: 杂志文章,评审

    doi:10.1080/21655979.2018.1470720

    authors: Perez Rojo F,Nyman RKM,Johnson AAT,Navarro MP,Ryan MH,Erskine W,Kaur P

    更新日期:2018-01-01 00:00:00

  • Kinetic analysis of IgM monoclonal antibodies for determination of dengue sample concentration using SPR technique.

    abstract::Surface plasmon resonance (SPR) sensing is recently emerging as a valuable technique for measuring the binding constants, association and dissociation rate constants, and stoichimetry for a binding interaction kinetics in a number of emerging biological areas. This technique can be applied to the study of immune syste...

    journal_title:Bioengineered

    pub_type: 杂志文章

    doi:10.1080/21655979.2016.1223413

    authors: Jahanshahi P,Wei Q,Jie Z,Ghomeishi M,Sekaran SD,Mahamd Adikan FR

    更新日期:2017-05-04 00:00:00

  • Isolation of a novel deep-sea Bacillus circulus strain and uniform design for optimization of its anti-aflatoxigenic bioactive metabolites production.

    abstract::The deep-sea bacterium strain FA13 was isolated from the sediment of the South Atlantic Ocean and identified as Bacillus circulans based on 16S ribosomal DNA sequence. Through liquid fermentation with five media, the cell-free supernatant fermented with ISP2 showed the highest inhibition activities against mycelial gr...

    journal_title:Bioengineered

    pub_type: 杂志文章

    doi:10.1080/21655979.2019.1586055

    authors: Zhou Y,Wang J,Gao X,Wang K,Wang W,Wang Q,Yan P

    更新日期:2019-12-01 00:00:00

  • Downregulation of long non-coding RNA LINC00460 inhibits the proliferation, migration and invasion, and promotes apoptosis of pancreatic cancer cells via modulation of the miR-320b/ARF1 axis.

    abstract::Pancreatic adenocarcinoma (PAAD) ranks among the most lethal cancers worldwide with high mortality. A marked increase in the level of long non-coding RNA LINC00460 was reported in PAAD patients, in comparison with the healthy controls. However, the underlying mechanisms of the above phenomenon are not yet well underst...

    journal_title:Bioengineered

    pub_type: 杂志文章

    doi:10.1080/21655979.2020.1863035

    authors: Cheng J,Lou Y,Jiang K

    更新日期:2021-12-01 00:00:00

  • Genetic engineering of microorganisms for biodiesel production.

    abstract::Biodiesel, as one type of renewable energy, is an ideal substitute for petroleum-based diesel fuel and is usually made from triacylglycerides by transesterification with alcohols. Biodiesel production based on microbial fermentation aiming to establish more efficient, less-cost and sustainable biodiesel production str...

    journal_title:Bioengineered

    pub_type: 杂志文章,评审

    doi:10.4161/bioe.23114

    authors: Lin H,Wang Q,Shen Q,Zhan J,Zhao Y

    更新日期:2013-09-01 00:00:00

  • Kinetic and thermodynamic characterization of a novel low-temperature-active xylanase from Arthrobacter sp. GN16 isolated from the feces of Grus nigricollis.

    abstract::We previously presented the cloning, heterologous expression, and characterization of a novel multidomain endoxylanase from Arthrobacter sp. GN16 isolated from the feces of Grus nigricollis. Molecular and biochemical characterization studies indicate that the glycoside hydrolase (GH) family 10 domain at the N-terminus...

    journal_title:Bioengineered

    pub_type: 杂志文章

    doi:10.1080/21655979.2014.1004021

    authors: Zhou J,Liu Y,Shen J,Zhang R,Tang X,Li J,Wang Y,Huang Z

    更新日期:2015-01-01 00:00:00

  • Penaeus orientolis prawn freshness rapid determination method based on electronic nose and non-linear stochastic resonance technique.

    abstract::In this paper, Penaeus orientolis prawn freshness rapid determination method using electronic nose (e-nose) and non-linear data processing technique is studied. E-nose responses to prawns stored at 4 °C are measured. Meanwhile, physical/chemical indexes (firmness, pH, total volatile basic nitrogen (TVB-N), total viabl...

    journal_title:Bioengineered

    pub_type: 杂志文章

    doi:10.4161/21655979.2014.993294

    authors: Wei L,Yuanyuan H,Yanping C,Jiaojiao J,Guohua H

    更新日期:2015-01-01 00:00:00

  • Secretion of slow-folding proteins by a Type 1 secretion system.

    abstract::Protein production through dedicated secretion systems might offer an potential alternative to the conventional cytoplasmical expression. The application of Type 1 secretion systems of Gram-negative bacteria, however, where often not successful in the past for a wide range of proteins. Recently, two studies using the ...

    journal_title:Bioengineered

    pub_type: 杂志文章

    doi:10.4161/bioe.20712

    authors: Schwarz CK,Lenders MH,Smits SH,Schmitt L

    更新日期:2012-09-01 00:00:00

  • Chaperones in maturation of molybdoenzymes: Why specific is better than general?

    abstract::Molybdoenzymes play essential functions in living organisms and, as a result, in various geochemical cycles. It is thus crucial to understand how these complex proteins become highly efficient enzymes able to perform a wide range of catalytic activities. It has been established that specific chaperones are involved du...

    journal_title:Bioengineered

    pub_type: 杂志文章

    doi:10.1080/21655979.2016.1218579

    authors: Lemaire ON,Bouillet S,Méjean V,Iobbi-Nivol C,Genest O

    更新日期:2017-03-04 00:00:00

  • Regulatory roles of 24-epibrassinolide in tolerance of Acacia gerrardii Benth to salt stress.

    abstract::This experiment aimed to investigate the role of 24-epibrassinolide (EBL) against NaCl-induced salinity stress in Acacia gerrardii Benth. NaCl (200 mM) imparted deleterious effects on the growth and chlorophyll contents of A. gerrardii, but foliar application of EBL (1.0 mg/l; each plant received 2.5 ml) mitigated the...

    journal_title:Bioengineered

    pub_type: 杂志文章

    doi:10.1080/21655979.2017.1297348

    authors: Abd Allah EF,Alqarawi AA,Hashem A,Wirth S,Egamberdieva D

    更新日期:2018-01-01 00:00:00

  • Characteristics of immune cell infiltration and associated diagnostic biomarkers in ulcerative colitis: results from bioinformatics analysis.

    abstract::Ulcerative colitis (UC) is a type of refractory and recurrent inflammatory disorder that occurs in colon and rectum. Immune cell infiltration plays a critical role in UC progression; therefore, this study aims to explore potential biomarkers for UC and to analyze characteristics of immune cell infiltration based on th...

    journal_title:Bioengineered

    pub_type: 杂志文章

    doi:10.1080/21655979.2020.1863016

    authors: Xue G,Hua L,Zhou N,Li J

    更新日期:2021-12-01 00:00:00

  • Implications of endophytic microbiota in Camellia sinensis: a review on current understanding and future insights.

    abstract::Endophytic fungi and bacteria are the most ubiquitous and representative commensal members that have been studied so far in various higher plants. Within colonization and interaction with their host plants, endophytic microbiota are reportedly to modulate not only the host's growth but also holobiont resilience to abi...

    journal_title:Bioengineered

    pub_type: 杂志文章

    doi:10.1080/21655979.2020.1816788

    authors: Xie H,Feng X,Wang M,Wang Y,Kumar Awasthi M,Xu P

    更新日期:2020-12-01 00:00:00

  • Development of an immune-related prognostic index associated with osteosarcoma.

    abstract::Tumor immunity is closely associated with the prognosis of tumors, including osteosarcoma (OS). The aim of the present study was to construct an immune-related prognostic index (PI) to predict the prognosis of OS. Herein, OS expression data were sourced from the Therapeutically Applicable Research to Generate Effectiv...

    journal_title:Bioengineered

    pub_type: 杂志文章

    doi:10.1080/21655979.2020.1864096

    authors: Yin CD,Hou YL,Liu XR,He YS,Wang XP,Li CJ,Tan XH,Liu J

    更新日期:2021-12-01 00:00:00

  • From stale bread and brewers spent grain to a new food source using edible filamentous fungi.

    abstract::By-products from the food sector with a high load of organic matter present both a waste-handling problem related to expenses and to the environment, yet also an opportunity. This study aims to increase the value of stale bread and brewers spent grain (BSG) by re-introducing these residues to the food production chain...

    journal_title:Bioengineered

    pub_type: 杂志文章

    doi:10.1080/21655979.2020.1768694

    authors: Gmoser R,Fristedt R,Larsson K,Undeland I,Taherzadeh MJ,Lennartsson PR

    更新日期:2020-12-01 00:00:00

  • LINC00528 regulates myocardial infarction by targeting the miR-143-3p/COX-2 axis.

    abstract::This study is aimed to explore the roles of LINC00528 in myocardial infarction (MI) progression. Quantitative real-time PCR showed that the expression of LINC00528 and COX-2 was upregulated while miR-143-3p expression was down-regulated in post-MI cells. In function assays, LINC00528 suppression promoted post-MI cells...

    journal_title:Bioengineered

    pub_type: 杂志文章

    doi:10.1080/21655979.2019.1704535

    authors: Liu K,Zhao D,Wang D

    更新日期:2020-12-01 00:00:00