Abstract:
:Circular RNA (circRNA) circ-LRP6 was recently proven to be a pivotal player in various human diseases. Nevertheless, its role in esophageal squamous cell cancer (ESCC) remains unknown. In the current study, we investigated the expression level, biological function and mechanism of circ-LRP6 in ESCC. Circ-LRP6 was significantly upregulated in ESCC tissues and correlated with malignant clinicopathological characteristics and poor prognosis. Knockdown of circ-LRP6 evidently reduced ESCC cell viability, colony formation and invasion. Circ-LRP6 was mainly located in the cytoplasm and could sponge miR-182 to increase the expression of Myc, a well-documented proto-oncogene. Importantly, circ-LRP6 depletion significantly retarded tumor growth in vivo. And silencing of miR-182 or overexpression of Myc effectively rescued the attenuated aggressive phenotype of ESCC cells caused by circ-LRP6 knockdown. Therefore, our data indicate that circ-LRP6 is a novel oncogenic circRNA in ESCC, targeting the circ-LRP6/miR-182/Myc signaling may be a promising therapeutic approach for ESCC patients.
journal_name
Bioengineeredjournal_title
Bioengineeredauthors
Wang J,Zhu W,Tao G,Wang Wdoi
10.1080/21655979.2020.1809922subject
Has Abstractpub_date
2020-12-01 00:00:00pages
932-938issue
1eissn
2165-5979issn
2165-5987journal_volume
11pub_type
杂志文章相关文献
Bioengineered文献大全abstract::Microbial enzymes have been used in a large number of fields, such as chemical, agricultural and biopharmaceutical industries. The enzyme production rate and yield are the main factors to consider when choosing the appropriate expression system for the production of recombinant proteins. Recombinant enzymes have been ...
journal_title:Bioengineered
pub_type: 杂志文章,评审
doi:10.4161/bioe.24761
更新日期:2013-07-01 00:00:00
abstract::The bioengineering tools have significant advantages through less time-consuming and utilized as a promising stage for the production of pharmaceutical bioproducts under the single platform. This review highlighted the advantages and current improvement in the plant, animal and microbial bioengineering tools and outli...
journal_title:Bioengineered
pub_type: 杂志文章,评审
doi:10.1080/21655979.2019.1682108
更新日期:2019-12-01 00:00:00
abstract::Extraction of sugar is the rate-limiting step in converting unpretreated biomass into value-added products through microbial fermentation. Both anaerobic fungi and anaerobic bacteria have evolved to produce large multi-cellulase complexes referred to as cellulosomes, which are powerful machines for biomass deconstruct...
journal_title:Bioengineered
pub_type: 杂志文章,评审
doi:10.1080/21655979.2015.1060379
更新日期:2015-01-01 00:00:00
abstract::A simple procedure for recovering biodegradable polymer from bacterial cells has been developed using economical and environmentally friendly solvent or chemicals. Recombinant bacterium, Cupriavidus necator harboring pBBR1MCS-C2 plasmid polyhydroxyalkanoate (PHA) synthase gene was used for the production of copolymer ...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.4161/bioe.22350
更新日期:2013-03-01 00:00:00
abstract::D-xylonate dehydratase YjhG from Escherichia coli can convert D-xylonate into 2-keto-3-deoxy- D-xylonate (KDX), and is a key enzyme in the biosynthesis of 1,2,4-butanetriol and other chemicals. However, the biochemical properties of YjhG still remain unknown. In this study, the activity assay method for YjhG was estab...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2015.1040208
更新日期:2015-01-01 00:00:00
abstract::The ascomycete Ophiostoma piceae produces a sterol esterase (OPE) with high affinity toward p-nitrophenol, glycerol, and sterol esters. Recently, this enzyme has been heterologously expressed in the methylotrophic yeast Pichia pastoris under the AOX1 methanol-inducible promoter (PAOX1) using sorbitol as co-susbtrate, ...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.4161/bioe.22818
更新日期:2013-07-01 00:00:00
abstract::In this study, a total of six fungal samples were isolated from apple, strawberry and orange pulp. DNA sequence analysis was used as molecular identification method. ITS region was aligned in DNA sequence analysis, and an algorithm sequence similarity was done using BLAST (Basic Local Alignment Search Tool) program to...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2020.1739379
更新日期:2020-12-01 00:00:00
abstract::Pancreatic adenocarcinoma (PAAD) ranks among the most lethal cancers worldwide with high mortality. A marked increase in the level of long non-coding RNA LINC00460 was reported in PAAD patients, in comparison with the healthy controls. However, the underlying mechanisms of the above phenomenon are not yet well underst...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2020.1863035
更新日期:2021-12-01 00:00:00
abstract::Epstein-Barr virus (EBV) is an oncogenic virus that infects more than 90% of the world's population. The proteins and miRNAs encoded by EBV are involved in multiple human malignancies. Recently R-resistance RNA-seq demonstrated that EBV-encoded circular RNAs. The current research aims to explore their functions in EBV...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2019.1679698
更新日期:2019-12-01 00:00:00
abstract::The detecting mechanism of carbon nanotubes gas sensor based on multi-stable stochastic resonance (MSR) model was studied in this paper. A numerically stimulating model based on MSR was established. And gas-ionizing experiment by adding electronic white noise to induce 1.65 MHz periodic component in the carbon nanotub...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2015.1058453
更新日期:2015-01-01 00:00:00
abstract::As the second most common gynecologic malignant tumors with a high mortality rate, cervical cancer jeopardizes women's life worldwide. The low cure rate in cervical cancer patients is mainly attributed to the lack of effective therapies. One feasible novel strategy is to develop immune-based approaches such as adoptiv...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2016.1230573
更新日期:2017-07-04 00:00:00
abstract::In this study, the conditions of extraction of loquat flowers polyphenolics were optimized through response surface methodology (RSM). Proper extraction conditions were: solid to liquid ratio 1 g per 50 mL and ethanol concentration 50% at 61°C for 9 min. Furthermore, the antioxidant and anti-polyphenol oxidase (PPO) a...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2020.1735604
更新日期:2020-12-01 00:00:00
abstract::Protein production through dedicated secretion systems might offer an potential alternative to the conventional cytoplasmical expression. The application of Type 1 secretion systems of Gram-negative bacteria, however, where often not successful in the past for a wide range of proteins. Recently, two studies using the ...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.4161/bioe.20712
更新日期:2012-09-01 00:00:00
abstract::Alkaline pectinase has important applications in the pretreatment of waste water from food processing and in both the fabric and paper industries. In this study, a 2-level factorial design was used to screen significant factors that affect the activity of alkaline pectinase, and the response surface methodology (RSM) ...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2017.1292188
更新日期:2017-09-03 00:00:00
abstract::The anti-inflammatory cytokine interleukin-10 and its viral homologs were chosen as model proteins for the development of drug delivery systems based on probiotic carriers like E. coli Nissle 1917, E. coli G3/10, and Saccharomyces boulardii. Exterior cytokine secretion was achieved by a modified E. coli hemolysin tran...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.4161/bioe.22646
更新日期:2013-05-01 00:00:00
abstract::Mammalian collagen has been widely used as a biomedical material. Nevertheless, there are still concerns about the variability between preparations, particularly with the possibility that the products may transmit animal-based diseases. Many groups have examined the possible application of bioengineered mammalian coll...
journal_title:Bioengineered
pub_type: 杂志文章,评审
doi:10.4161/bioe.28791
更新日期:2014-07-01 00:00:00
abstract::In this paper, Penaeus orientolis prawn freshness rapid determination method using electronic nose (e-nose) and non-linear data processing technique is studied. E-nose responses to prawns stored at 4 °C are measured. Meanwhile, physical/chemical indexes (firmness, pH, total volatile basic nitrogen (TVB-N), total viabl...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.4161/21655979.2014.993294
更新日期:2015-01-01 00:00:00
abstract::The plant-specific RING-domain finger proteins play important roles in plant development and stress responses. We recently identified and functionally characterized a stress-induced gene OsSRFP1 (Oryza sativa Stress-related RING Finger Protein 1) from rice. We showed evidences of the biotechnological potential of the ...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2015.1131368
更新日期:2016-01-01 00:00:00
abstract::This paper investigates the application of anodic porous alumina as an advancement on chip laboratory for gene expressions. The surface was prepared by a suitable electrolytic process to obtain a regular distribution of deep micrometric holes and printed bypen robot tips under standard conditions. The gene expression ...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.4161/bioe.25278
更新日期:2013-09-01 00:00:00
abstract::Herein, I track the evolution of synthetic biology from its earliest incarnations more than 50 years ago, through the DIYbio revolution, to the next 50 years. ...
journal_title:Bioengineered
pub_type: 社论
doi:10.4161/bioe.28317
更新日期:2014-03-01 00:00:00
abstract::Downregulation of lncRNA H19 (H19) expression is associated with an unfavorable prognosis in some cancers. However, little was known as to whether there was an association between H19 and minimally invasive follicular thyroid carcinoma (MI-FTC). In our study, we used quantitative real-time polymerase chain reaction (q...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2019.1658489
更新日期:2019-12-01 00:00:00
abstract::The evolution of natural modular proteins and domain swapping by protein engineers have shown the disruptive potential of non-homologous recombination to create proteins with novel functions or traits. Bacteriophage endolysins, cellulosomes and polyketide synthases are 3 examples of natural modular proteins with each ...
journal_title:Bioengineered
pub_type: 杂志文章,评审
doi:10.1080/21655979.2016.1222993
更新日期:2017-05-04 00:00:00
abstract::For somatic gene therapy of hemophilia B, hepatocytes as the main cellular host for expression of hFIX are attractive targets. In gene therapy protocols, an efficient expression vector equipped with cis-regulatory elements such as introns is required. With this in mind, hFIX-expressing plasmids equipped with different...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2016.1207018
更新日期:2016-11-01 00:00:00
abstract:NANNOCHLOROPSIS:sp. is a green alga that is widely used in the aquaculture industry as a feed in Malaysia, but genetic engineering studies of this alga are still underexplored even though there is a growing interest in microalgae genetic engineering for various industrial purposes. This study aims to investigate the ef...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2020.1822106
更新日期:2020-12-01 00:00:00
abstract::The deep-sea bacterium strain FA13 was isolated from the sediment of the South Atlantic Ocean and identified as Bacillus circulans based on 16S ribosomal DNA sequence. Through liquid fermentation with five media, the cell-free supernatant fermented with ISP2 showed the highest inhibition activities against mycelial gr...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2019.1586055
更新日期:2019-12-01 00:00:00
abstract::Atherosclerosis enables to alter not only the microstructural but also the physical properties of the arterial walls by plaque forming. Few studies so far have been conducted to calculate the isotropic or anisotropic mechanical properties of the healthy and atherosclerotic human coronary arteries. To date there is a p...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2016.1212134
更新日期:2017-03-04 00:00:00
abstract::The successful treatment of keloids is a great challenge in the plastic surgery field. Activating transcription factor 3 (ATF3) is discovered as an adaptive responsive gene, which plays a critical role in fibroblast activation. This study aimed to investigate the expression and biological role of ATF3 in the pathogene...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2020.1860491
更新日期:2021-12-01 00:00:00
abstract::We previously presented the cloning, heterologous expression, and characterization of a novel multidomain endoxylanase from Arthrobacter sp. GN16 isolated from the feces of Grus nigricollis. Molecular and biochemical characterization studies indicate that the glycoside hydrolase (GH) family 10 domain at the N-terminus...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2014.1004021
更新日期:2015-01-01 00:00:00
abstract::Cyanobacteria are Gram-negative photoautotrophic prokaryotes and have shown great importance to the Earth's ecology. Based on their capability in oxygenic photosynthesis and genetic merits, they can be engineered as microbial chassis for direct conversion of carbon dioxide to value-added biofuels and chemicals. In the...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2020.1837458
更新日期:2020-12-01 00:00:00
abstract::Rhizoremediation is one of the most accepted, cost-effective bioremediation techniques focusing on the application of rhizospheric microorganisms in combination with plants for the remediation of organic and inorganic pollutants from the contaminated sites. This work focuses on isolation and identification of metal re...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2020.1728034
更新日期:2020-12-01 00:00:00