Improving health from the inside: Use of engineered intestinal microorganisms as in situ cytokine delivery system.

abstract：:In this paper, Penaeus orientolis prawn freshness rapid determination method using electronic nose (e-nose) and non-linear data processing technique is studied. E-nose responses to prawns stored at 4 °C are measured. Meanwhile, physical/chemical indexes (firmness, pH, total volatile basic nitrogen (TVB-N), total viabl...

journal_title：Bioengineered

authors： Wei L,Yuanyuan H,Yanping C,Jiaojiao J,Guohua H

更新日期：2015-01-01 00:00:00

abstract：:MiR-25 is a well-documented oncogenic miRNA implicated in esophageal squamous cell carcinoma (ESCC) development, progression and metastasis. However, whether and how miR-25 is involved in the development and metastasis of ESCC remain un-addressed. By using qRT-PCR analysis to compare levels of miR-25 in ESCC tissues w...

journal_title：Bioengineered

authors： Liu B,Li X,Li C,Xu R,Sun X

更新日期：2019-12-01 00:00:00

abstract：:The plant-specific RING-domain finger proteins play important roles in plant development and stress responses. We recently identified and functionally characterized a stress-induced gene OsSRFP1 (Oryza sativa Stress-related RING Finger Protein 1) from rice. We showed evidences of the biotechnological potential of the ...

journal_title：Bioengineered

authors： Fang H,Meng Q,Zhang H,Huang J

更新日期：2016-01-01 00:00:00

abstract：:A genetic recombinant Saccharomyces cerevisiae starter with high ethanol tolerance capacities was constructed. In this study, the gene of trehalose-6-phosphate synthase (encoded by tps1), which catalyzes the first step in trehalose synthesis, was cloned and overexpressed in S. cerevisiae. Moreover, the gene of neutral...

journal_title：Bioengineered

authors： Divate NR,Chen GH,Wang PM,Ou BR,Chung YC

更新日期：2016-11-01 00:00:00

abstract：:We previously presented the cloning, heterologous expression, and characterization of a novel multidomain endoxylanase from Arthrobacter sp. GN16 isolated from the feces of Grus nigricollis. Molecular and biochemical characterization studies indicate that the glycoside hydrolase (GH) family 10 domain at the N-terminus...

journal_title：Bioengineered

authors： Zhou J,Liu Y,Shen J,Zhang R,Tang X,Li J,Wang Y,Huang Z

更新日期：2015-01-01 00:00:00

abstract：:Economically feasible production of second-generation biofuels requires efficient co-fermentation of pentose and hexose sugars in lignocellulosic hydrolysates under very harsh conditions. Baker's yeast is an excellent, traditionally used ethanol producer but is naturally not able to utilize pentoses. This is due to th...

journal_title：Bioengineered

authors： Oreb M,Dietz H,Farwick A,Boles E

更新日期：2012-11-01 00:00:00

abstract：:Bacterial CotA-laccases exhibit higher activity in alkaline pH and salt concentration conditions compared to laccases from white-rot fungi. They are considered as green catalysts in decolorizing of industrial dyes. However, CotA-laccases are limited due to the low yield and catalytic efficiency as the spore-bound natu...

journal_title：Bioengineered

authors： Ouyang F,Zhao M

更新日期：2019-12-01 00:00:00

abstract：:Endophytic fungi and bacteria are the most ubiquitous and representative commensal members that have been studied so far in various higher plants. Within colonization and interaction with their host plants, endophytic microbiota are reportedly to modulate not only the host's growth but also holobiont resilience to abi...

journal_title：Bioengineered

authors： Xie H,Feng X,Wang M,Wang Y,Kumar Awasthi M,Xu P

更新日期：2020-12-01 00:00:00

abstract：:Herein, I track the evolution of synthetic biology from its earliest incarnations more than 50 years ago, through the DIYbio revolution, to the next 50 years. ...

journal_title：Bioengineered

authors： Sleator RD

更新日期：2014-03-01 00:00:00

abstract：:Mammalian collagen has been widely used as a biomedical material. Nevertheless, there are still concerns about the variability between preparations, particularly with the possibility that the products may transmit animal-based diseases. Many groups have examined the possible application of bioengineered mammalian coll...

journal_title：Bioengineered

authors： Ramshaw JA,Werkmeister JA,Dumsday GJ

更新日期：2014-07-01 00:00:00

abstract：:A simple procedure for recovering biodegradable polymer from bacterial cells has been developed using economical and environmentally friendly solvent or chemicals. Recombinant bacterium, Cupriavidus necator harboring pBBR1MCS-C2 plasmid polyhydroxyalkanoate (PHA) synthase gene was used for the production of copolymer ...

journal_title：Bioengineered

authors： Anis SN,Iqbal NM,Kumar S,Al-Ashraf A

更新日期：2013-03-01 00:00:00

abstract：:For somatic gene therapy of hemophilia B, hepatocytes as the main cellular host for expression of hFIX are attractive targets. In gene therapy protocols, an efficient expression vector equipped with cis-regulatory elements such as introns is required. With this in mind, hFIX-expressing plasmids equipped with different...

journal_title：Bioengineered

authors： Azadbakhsh AS,Sam MR,Farokhi F

更新日期：2016-11-01 00:00:00

abstract：:Molybdoenzymes play essential functions in living organisms and, as a result, in various geochemical cycles. It is thus crucial to understand how these complex proteins become highly efficient enzymes able to perform a wide range of catalytic activities. It has been established that specific chaperones are involved du...

journal_title：Bioengineered

authors： Lemaire ON,Bouillet S,Méjean V,Iobbi-Nivol C,Genest O

更新日期：2017-03-04 00:00:00

abstract：:Extraction of sugar is the rate-limiting step in converting unpretreated biomass into value-added products through microbial fermentation. Both anaerobic fungi and anaerobic bacteria have evolved to produce large multi-cellulase complexes referred to as cellulosomes, which are powerful machines for biomass deconstruct...

journal_title：Bioengineered

authors： Gilmore SP,Henske JK,O'Malley MA

更新日期：2015-01-01 00:00:00

abstract：:In this study, the conditions of extraction of loquat flowers polyphenolics were optimized through response surface methodology (RSM). Proper extraction conditions were: solid to liquid ratio 1 g per 50 mL and ethanol concentration 50% at 61°C for 9 min. Furthermore, the antioxidant and anti-polyphenol oxidase (PPO) a...

journal_title：Bioengineered

authors： Huang GL,Ma JJ,Sui SY,Wang YN

更新日期：2020-12-01 00:00:00

abstract：:The edible filamentous fungi are hot candidate for future supply of functional food and feed with e.g. protein, essential amino acids, and compounds with immunostimulant activity. L-carnitine that plays a crucial role in energy metabolism represents a functional compound normally produced by Zygomycetes filamentous fu...

journal_title：Bioengineered

authors： Rousta N,Ferreira JA,Taherzadeh MJ

更新日期：2021-12-01 00:00:00

abstract：:Botulinum toxin type A can temporarily inhibit muscle contraction. Currently, physicians administer this toxin as a bio-drug in treatment of some muscle contraction disorders. TAT-BoNT/A(1-448) is a functional recombinant protein derived from botulinum toxin light chain. Unlike the full length botulinum toxin, TAT-BoN...

journal_title：Bioengineered

authors： Saffarian P,Peerayeh SN,Amani J,Ebrahimi F,Sedighianrad H,Halabian R,Imani Fooladi AA

更新日期：2016-11-01 00:00:00

abstract：:Kidney renal clear cell carcinoma (ccRCC) is a malignant tumor originating from renal tubular epithelium, lncRNAs can regulate the occurrence and development of EMT by targeting EMT transcription factors. We constructed a new survival signature based on EMT-related differentially expressed lncRNAs obtained from The Ca...

journal_title：Bioengineered

authors： Cheng YB,Li S,Zhang YP,Zhu ZH,Zhao N

更新日期：2021-01-31 00:00:00

abstract：:Epstein-Barr virus (EBV) is an oncogenic virus that infects more than 90% of the world's population. The proteins and miRNAs encoded by EBV are involved in multiple human malignancies. Recently R-resistance RNA-seq demonstrated that EBV-encoded circular RNAs. The current research aims to explore their functions in EBV...

journal_title：Bioengineered

authors： Qiao Y,Zhao X,Liu J,Yang W

更新日期：2019-12-01 00:00:00

abstract：:Atherosclerosis enables to alter not only the microstructural but also the physical properties of the arterial walls by plaque forming. Few studies so far have been conducted to calculate the isotropic or anisotropic mechanical properties of the healthy and atherosclerotic human coronary arteries. To date there is a p...

journal_title：Bioengineered

authors： Karimi A,Rahmati SM,Sera T,Kudo S,Navidbakhsh M

更新日期：2017-03-04 00:00:00

abstract：:Downregulation of lncRNA H19 (H19) expression is associated with an unfavorable prognosis in some cancers. However, little was known as to whether there was an association between H19 and minimally invasive follicular thyroid carcinoma (MI-FTC). In our study, we used quantitative real-time polymerase chain reaction (q...

journal_title：Bioengineered

authors： Dai Y,Miao Y,Zhu Q,Gao M,Hao F

更新日期：2019-12-01 00:00:00

abstract：:The human chromogranin A-derived peptide CGA-N12, which is composed of 12 amino acid residues with the sequence ALQGAKERAHQQ, showed strong antifungal activity and the least hemolytic activity in previous studies. However, synthetic peptides are relatively expensive to produce. Recombinant expression of peptides in th...

journal_title：Bioengineered

authors： Li X,Fan Y,Lin Q,Luo J,Huang Y,Bao Y,Xu L

更新日期：2020-12-01 00:00:00

abstract：:The main treatment option for Hemophilia A/B patients involves the administration of recombinant coagulation factors on-demand or in a prophylactic approach. Despite the safety and efficacy of this replacement therapy, the development of antibodies against the coagulation factor infused, which neutralize the procoagul...

journal_title：Bioengineered

authors： Swiech K,Picanço-Castro V,Covas DT

更新日期：2017-09-03 00:00:00

abstract：:We demonstrated previously that expression of Rhizomucor miehei lipase (RML) in Pichia pastoris could be significantly increased by addition of gene propeptide, optimized signal peptide codons and manipulation of gene dosage. In this study, effects of various strategies on the protein synthesis and secretion pathways ...

journal_title：Bioengineered

authors： Huang J,Wang Q,Bu W,Chen L,Yang Z,Zheng W,Li Y,Li J

更新日期：2019-12-01 00:00:00

abstract：:In contrast to conventional, molecular medicine that focuses on targeting specific pathways, stem cell therapy aims to perturb many related mechanisms in order to derive therapeutic benefit. This emerging modality is inherently complex due to the variety of cell types that can be used, delivery approaches that need to...

journal_title：Bioengineered

authors： Momeni A,Neelamegham S,Parashurama N

更新日期：2017-07-04 00:00:00

abstract：:We report the molecular cloning, expression, and single-step homogeneous purification of RNA polymerase II subunit RPB5 from Saccharomyces cerevisiae. RPB5 is a 210 amino acid nuclear protein that functions as the fifth largest subunit of polymerase II and plays a central role in transcription. The gene that codes for...

journal_title：Bioengineered

authors： Chhetri G,Ghosh A,Chinta R,Akhtar S,Tripathi T

更新日期：2015-01-01 00:00:00

abstract：:This study is aimed to explore the roles of LINC00528 in myocardial infarction (MI) progression. Quantitative real-time PCR showed that the expression of LINC00528 and COX-2 was upregulated while miR-143-3p expression was down-regulated in post-MI cells. In function assays, LINC00528 suppression promoted post-MI cells...

journal_title：Bioengineered

authors： Liu K,Zhao D,Wang D

更新日期：2020-12-01 00:00:00

abstract：:Cyanobacteria are Gram-negative photoautotrophic prokaryotes and have shown great importance to the Earth's ecology. Based on their capability in oxygenic photosynthesis and genetic merits, they can be engineered as microbial chassis for direct conversion of carbon dioxide to value-added biofuels and chemicals. In the...

journal_title：Bioengineered

authors： Wang F,Gao Y,Yang G

更新日期：2020-12-01 00:00:00

abstract：:Organ transplantation can offer a curative option for patients with end stage organ failure. Unfortunately the treatment is severely limited by the availability of donor organs. Organ bioengineering could provide a solution to the worldwide critical organ shortage. The majority of protocols to date have employed the u...

journal_title：Bioengineered

authors： Welman T,Michel S,Segaren N,Shanmugarajah K

更新日期：2015-01-01 00:00:00

abstract：:Microbial enzymes have been used in a large number of fields, such as chemical, agricultural and biopharmaceutical industries. The enzyme production rate and yield are the main factors to consider when choosing the appropriate expression system for the production of recombinant proteins. Recombinant enzymes have been ...

journal_title：Bioengineered

authors： Liu L,Yang H,Shin HD,Chen RR,Li J,Du G,Chen J

更新日期：2013-07-01 00:00:00