Engineering ribosomal leaky scanning and upstream open reading frames for precise control of protein translation.

abstract：:Protein production through dedicated secretion systems might offer an potential alternative to the conventional cytoplasmical expression. The application of Type 1 secretion systems of Gram-negative bacteria, however, where often not successful in the past for a wide range of proteins. Recently, two studies using the ...

journal_title：Bioengineered

authors： Schwarz CK,Lenders MH,Smits SH,Schmitt L

更新日期：2012-09-01 00:00:00

abstract：:The plant-specific RING-domain finger proteins play important roles in plant development and stress responses. We recently identified and functionally characterized a stress-induced gene OsSRFP1 (Oryza sativa Stress-related RING Finger Protein 1) from rice. We showed evidences of the biotechnological potential of the ...

journal_title：Bioengineered

authors： Fang H,Meng Q,Zhang H,Huang J

更新日期：2016-01-01 00:00:00

abstract：:Extraction of sugar is the rate-limiting step in converting unpretreated biomass into value-added products through microbial fermentation. Both anaerobic fungi and anaerobic bacteria have evolved to produce large multi-cellulase complexes referred to as cellulosomes, which are powerful machines for biomass deconstruct...

journal_title：Bioengineered

authors： Gilmore SP,Henske JK,O'Malley MA

更新日期：2015-01-01 00:00:00

abstract：:Endophytic fungi and bacteria are the most ubiquitous and representative commensal members that have been studied so far in various higher plants. Within colonization and interaction with their host plants, endophytic microbiota are reportedly to modulate not only the host's growth but also holobiont resilience to abi...

journal_title：Bioengineered

authors： Xie H,Feng X,Wang M,Wang Y,Kumar Awasthi M,Xu P

更新日期：2020-12-01 00:00:00

abstract：:D-xylonate dehydratase YjhG from Escherichia coli can convert D-xylonate into 2-keto-3-deoxy- D-xylonate (KDX), and is a key enzyme in the biosynthesis of 1,2,4-butanetriol and other chemicals. However, the biochemical properties of YjhG still remain unknown. In this study, the activity assay method for YjhG was estab...

journal_title：Bioengineered

authors： Jiang Y,Liu W,Cheng T,Cao Y,Zhang R,Xian M

更新日期：2015-01-01 00:00:00

abstract：:During the last few decades, Streptomycetes have shown to be a very important and adaptable group of bacteria for the production of various beneficial secondary metabolites. These secondary metabolites have been of great interest in academia and the pharmaceutical industries. To date, a vast variety of techniques and ...

journal_title：Bioengineered

authors： Bekker V,Dodd A,Brady D,Rumbold K

更新日期：2014-09-01 00:00:00

abstract：:Production of psychrophilic enzymes in the commonly used mesophilic expression systems is hampered by low intrinsic stability of the recombinant enzymes at the optimal host growth temperatures. Unless strategies for low-temperature expression are advanced, research on psychrophilic enzymes may end up being biased towa...

journal_title：Bioengineered

authors： Bjerga GE,Lale R,Williamson AK

更新日期：2016-01-01 00:00:00

abstract：:The detecting mechanism of carbon nanotubes gas sensor based on multi-stable stochastic resonance (MSR) model was studied in this paper. A numerically stimulating model based on MSR was established. And gas-ionizing experiment by adding electronic white noise to induce 1.65 MHz periodic component in the carbon nanotub...

journal_title：Bioengineered

authors： Jingyi Z

更新日期：2015-01-01 00:00:00

abstract：:In this paper, Penaeus orientolis prawn freshness rapid determination method using electronic nose (e-nose) and non-linear data processing technique is studied. E-nose responses to prawns stored at 4 °C are measured. Meanwhile, physical/chemical indexes (firmness, pH, total volatile basic nitrogen (TVB-N), total viabl...

journal_title：Bioengineered

authors： Wei L,Yuanyuan H,Yanping C,Jiaojiao J,Guohua H

更新日期：2015-01-01 00:00:00

abstract：:Illicium verum (badiane or star anise), Crataegus oxyacantha ssp monogyna (hawthorn) and Allium cepa (onion), have traditionnally been used as medicinal plants in Algeria. This study showed that the outer layer of onion is rich in flavonols with contents of 103 ± 7.90 µg/g DW (red variety) and 17.3 ± 0.69 µg/gDW (whit...

journal_title：Bioengineered

authors： Benmalek Y,Yahia OA,Belkebir A,Fardeau ML

更新日期：2013-07-01 00:00:00

abstract：:Herein, I track the evolution of synthetic biology from its earliest incarnations more than 50 years ago, through the DIYbio revolution, to the next 50 years. ...

journal_title：Bioengineered

authors： Sleator RD

更新日期：2014-03-01 00:00:00

abstract：:Increasing evidence has shown that the tumor microenvironment (TME) plays an important role in tumor occurrence and development and can also affect patient prognosis. In this study, we screened key prognostic genes in the breast cancer (BC) TME by analyzing the immune and stromal scores of tumor samples to detect diff...

journal_title：Bioengineered

authors： Ye Q,Han X,Wu Z

更新日期：2020-12-01 00:00:00

abstract：:Economically feasible production of second-generation biofuels requires efficient co-fermentation of pentose and hexose sugars in lignocellulosic hydrolysates under very harsh conditions. Baker's yeast is an excellent, traditionally used ethanol producer but is naturally not able to utilize pentoses. This is due to th...

journal_title：Bioengineered

authors： Oreb M,Dietz H,Farwick A,Boles E

更新日期：2012-11-01 00:00:00

abstract：:Bovine chymosin is an important milk-clotting agent used in the manufacturing of cheeses. Currently, the production of recombinant proteins by genetically modified organisms is widespread, leading to greatly reduced costs. Lactococcus (L.) lactis, the model lactic acid bacterium, was considered a good candidate for he...

journal_title：Bioengineered

authors： Luerce TD,Azevedo MS,LeBlanc JG,Azevedo V,Miyoshi A,Pontes DS

更新日期：2014-01-01 00:00:00

abstract：:Surface plasmon resonance (SPR) sensing is recently emerging as a valuable technique for measuring the binding constants, association and dissociation rate constants, and stoichimetry for a binding interaction kinetics in a number of emerging biological areas. This technique can be applied to the study of immune syste...

journal_title：Bioengineered

authors： Jahanshahi P,Wei Q,Jie Z,Ghomeishi M,Sekaran SD,Mahamd Adikan FR

更新日期：2017-05-04 00:00:00

abstract：:Kidney renal clear cell carcinoma (ccRCC) is a malignant tumor originating from renal tubular epithelium, lncRNAs can regulate the occurrence and development of EMT by targeting EMT transcription factors. We constructed a new survival signature based on EMT-related differentially expressed lncRNAs obtained from The Ca...

journal_title：Bioengineered

authors： Cheng YB,Li S,Zhang YP,Zhu ZH,Zhao N

更新日期：2021-01-31 00:00:00

abstract：:The ascomycete Ophiostoma piceae produces a sterol esterase (OPE) with high affinity toward p-nitrophenol, glycerol, and sterol esters. Recently, this enzyme has been heterologously expressed in the methylotrophic yeast Pichia pastoris under the AOX1 methanol-inducible promoter (PAOX1) using sorbitol as co-susbtrate, ...

journal_title：Bioengineered

authors： Barba Cedillo V,Prieto A,Martínez MJ

更新日期：2013-07-01 00:00:00

abstract：:Molybdoenzymes play essential functions in living organisms and, as a result, in various geochemical cycles. It is thus crucial to understand how these complex proteins become highly efficient enzymes able to perform a wide range of catalytic activities. It has been established that specific chaperones are involved du...

journal_title：Bioengineered

authors： Lemaire ON,Bouillet S,Méjean V,Iobbi-Nivol C,Genest O

更新日期：2017-03-04 00:00:00

abstract：:MiR-25 is a well-documented oncogenic miRNA implicated in esophageal squamous cell carcinoma (ESCC) development, progression and metastasis. However, whether and how miR-25 is involved in the development and metastasis of ESCC remain un-addressed. By using qRT-PCR analysis to compare levels of miR-25 in ESCC tissues w...

journal_title：Bioengineered

authors： Liu B,Li X,Li C,Xu R,Sun X

更新日期：2019-12-01 00:00:00

abstract：:The successful treatment of keloids is a great challenge in the plastic surgery field. Activating transcription factor 3 (ATF3) is discovered as an adaptive responsive gene, which plays a critical role in fibroblast activation. This study aimed to investigate the expression and biological role of ATF3 in the pathogene...

journal_title：Bioengineered

authors： Wang XM,Liu XM,Wang Y,Chen ZY

更新日期：2021-12-01 00:00:00

abstract：:By-products from the food sector with a high load of organic matter present both a waste-handling problem related to expenses and to the environment, yet also an opportunity. This study aims to increase the value of stale bread and brewers spent grain (BSG) by re-introducing these residues to the food production chain...

journal_title：Bioengineered

authors： Gmoser R,Fristedt R,Larsson K,Undeland I,Taherzadeh MJ,Lennartsson PR

更新日期：2020-12-01 00:00:00

abstract：:Organ transplantation can offer a curative option for patients with end stage organ failure. Unfortunately the treatment is severely limited by the availability of donor organs. Organ bioengineering could provide a solution to the worldwide critical organ shortage. The majority of protocols to date have employed the u...

journal_title：Bioengineered

authors： Welman T,Michel S,Segaren N,Shanmugarajah K

更新日期：2015-01-01 00:00:00

abstract：:The straw can be degraded efficiently into humus by powerful enzymes from microorganisms, resulting in the accelerated circulation of N,P,K and other effective elements in ecological system. We isolated a strain through screening the straw degradation strains from natural humic straw in the low temperature area in nor...

journal_title：Bioengineered

authors： Zhang J,Hou H,Chen G,Wang S,Zhang J

更新日期：2016-09-02 00:00:00

abstract：:N-linked glycosylation is a primary source of heterogeneity associated with recombinant monoclonal antibodies and plays a key role in a myriad of drug properties associated with biological function. The glycosylation profile of recombinant monoclonal antibodies is influenced by an array of cell culture inputs which mu...

journal_title：Bioengineered

authors： Schilling M,Feng P,Sosic Z,Traviglia SL

更新日期：2020-12-01 00:00:00

abstract：:Ulcerative colitis (UC) is a type of refractory and recurrent inflammatory disorder that occurs in colon and rectum. Immune cell infiltration plays a critical role in UC progression; therefore, this study aims to explore potential biomarkers for UC and to analyze characteristics of immune cell infiltration based on th...

journal_title：Bioengineered

authors： Xue G,Hua L,Zhou N,Li J

更新日期：2021-12-01 00:00:00

abstract：:Mammalian collagen has been widely used as a biomedical material. Nevertheless, there are still concerns about the variability between preparations, particularly with the possibility that the products may transmit animal-based diseases. Many groups have examined the possible application of bioengineered mammalian coll...

journal_title：Bioengineered

authors： Ramshaw JA,Werkmeister JA,Dumsday GJ

更新日期：2014-07-01 00:00:00

abstract：:Pancreatic adenocarcinoma (PAAD) ranks among the most lethal cancers worldwide with high mortality. A marked increase in the level of long non-coding RNA LINC00460 was reported in PAAD patients, in comparison with the healthy controls. However, the underlying mechanisms of the above phenomenon are not yet well underst...

journal_title：Bioengineered

authors： Cheng J,Lou Y,Jiang K

更新日期：2021-12-01 00:00:00

abstract：:This invited commentary covers the period 1997-2012 and has seen changes in terminology that progressed from "basic" and "applied" to "translational" research. In the context of Bioengineered, these changes map readily onto the processes of identifying microbial characteristics appropriate for specific applications, i...

journal_title：Bioengineered

authors： Dunne C

更新日期：2013-07-01 00:00:00

abstract：:This study aimed to detect serum miR-203 expression levels in AML and explore its potential clinical significance. Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) was performed to measure the serum miR-203 levels in 134 patients with AML and 70 healthy controls. The results demonstrated that ser...

journal_title：Bioengineered

authors： Guo Y

更新日期：2019-12-01 00:00:00

abstract：:We demonstrated previously that expression of Rhizomucor miehei lipase (RML) in Pichia pastoris could be significantly increased by addition of gene propeptide, optimized signal peptide codons and manipulation of gene dosage. In this study, effects of various strategies on the protein synthesis and secretion pathways ...

journal_title：Bioengineered

authors： Huang J,Wang Q,Bu W,Chen L,Yang Z,Zheng W,Li Y,Li J

更新日期：2019-12-01 00:00:00