Abstract:
:Production of psychrophilic enzymes in the commonly used mesophilic expression systems is hampered by low intrinsic stability of the recombinant enzymes at the optimal host growth temperatures. Unless strategies for low-temperature expression are advanced, research on psychrophilic enzymes may end up being biased toward those that can be stably produced in commonly used mesophilic host systems. Two main strategies are currently being explored for the development of low-temperature expression in bacterial hosts: (i) low-temperature adaption of existing mesophilic expression systems, and (ii) development of new psychrophilic hosts. These developments include genetic engineering of the expression cassettes to optimize the promoter/operator systems that regulate heterologous expression. In this addendum we present our efforts in the development of such low-temperature expression systems, and speculate about future advancements in the field and potential applications.
journal_name
Bioengineeredjournal_title
Bioengineeredauthors
Bjerga GE,Lale R,Williamson AKdoi
10.1080/21655979.2015.1128589subject
Has Abstractpub_date
2016-01-01 00:00:00pages
33-8issue
1eissn
2165-5979issn
2165-5987journal_volume
7pub_type
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