Abstract:
:Biodiesel, as one type of renewable energy, is an ideal substitute for petroleum-based diesel fuel and is usually made from triacylglycerides by transesterification with alcohols. Biodiesel production based on microbial fermentation aiming to establish more efficient, less-cost and sustainable biodiesel production strategies is under current investigation by various start-up biotechnology companies and research centers. Genetic engineering plays a key role in the transformation of microbes into the desired cell factories with high efficiency of biodiesel production. Here, we present an overview of principal microorganisms used in the microbial biodiesel production and recent advances in metabolic engineering for the modification required. Overexpression or deletion of the related enzymes for de novo synthesis of biodiesel is highlighted with relevant examples.
journal_name
Bioengineeredjournal_title
Bioengineeredauthors
Lin H,Wang Q,Shen Q,Zhan J,Zhao Ydoi
10.4161/bioe.23114subject
Has Abstractpub_date
2013-09-01 00:00:00pages
292-304issue
5eissn
2165-5979issn
2165-5987pii
23114journal_volume
4pub_type
杂志文章,评审相关文献
Bioengineered文献大全abstract::The influenza A virus is a critical public health problem that causes epidemics and pandemics, and occurs widely all over the world. Various vaccines against the virus have not provided a solution to the problem. Different approaches, particularly M2e peptide-based vaccines, are available for developing universal vacc...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.4161/21655979.2014.969131
更新日期:2014-01-01 00:00:00
abstract::Cyanobacteria are Gram-negative photoautotrophic prokaryotes and have shown great importance to the Earth's ecology. Based on their capability in oxygenic photosynthesis and genetic merits, they can be engineered as microbial chassis for direct conversion of carbon dioxide to value-added biofuels and chemicals. In the...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2020.1837458
更新日期:2020-12-01 00:00:00
abstract::Molybdoenzymes play essential functions in living organisms and, as a result, in various geochemical cycles. It is thus crucial to understand how these complex proteins become highly efficient enzymes able to perform a wide range of catalytic activities. It has been established that specific chaperones are involved du...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2016.1218579
更新日期:2017-03-04 00:00:00
abstract::In this paper, Penaeus orientolis prawn freshness rapid determination method using electronic nose (e-nose) and non-linear data processing technique is studied. E-nose responses to prawns stored at 4 °C are measured. Meanwhile, physical/chemical indexes (firmness, pH, total volatile basic nitrogen (TVB-N), total viabl...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.4161/21655979.2014.993294
更新日期:2015-01-01 00:00:00
abstract::In contrast to conventional, molecular medicine that focuses on targeting specific pathways, stem cell therapy aims to perturb many related mechanisms in order to derive therapeutic benefit. This emerging modality is inherently complex due to the variety of cell types that can be used, delivery approaches that need to...
journal_title:Bioengineered
pub_type: 杂志文章,评审
doi:10.1080/21655979.2016.1233090
更新日期:2017-07-04 00:00:00
abstract::We have employed upstream open reading frames (uORFs) to systematically tune the translation levels of recombinant proteins. We present the design principles that guided the development of this technology and provide information that may help others in implementing synthetic uORFs for their own applications. We also r...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.4161/bioe.27607
更新日期:2014-05-01 00:00:00
abstract::This experiment aimed to investigate the role of 24-epibrassinolide (EBL) against NaCl-induced salinity stress in Acacia gerrardii Benth. NaCl (200 mM) imparted deleterious effects on the growth and chlorophyll contents of A. gerrardii, but foliar application of EBL (1.0 mg/l; each plant received 2.5 ml) mitigated the...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2017.1297348
更新日期:2018-01-01 00:00:00
abstract::Ulcerative colitis (UC) is a type of refractory and recurrent inflammatory disorder that occurs in colon and rectum. Immune cell infiltration plays a critical role in UC progression; therefore, this study aims to explore potential biomarkers for UC and to analyze characteristics of immune cell infiltration based on th...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2020.1863016
更新日期:2021-12-01 00:00:00
abstract::Mammalian collagen has been widely used as a biomedical material. Nevertheless, there are still concerns about the variability between preparations, particularly with the possibility that the products may transmit animal-based diseases. Many groups have examined the possible application of bioengineered mammalian coll...
journal_title:Bioengineered
pub_type: 杂志文章,评审
doi:10.4161/bioe.28791
更新日期:2014-07-01 00:00:00
abstract::We propose a mechanism of action for the betL* mutation which is based on DNA topology. Removing a single thymine residue from the betL σ(A) promoter's -10 and -35 spacer results in a 'twist'-mediated activation of transcription which accounts for the osmotolerance phenotype observed for strains expressing betL*. ...
journal_title:Bioengineered
pub_type: 评论,杂志文章
doi:10.1080/21655979.2016.1171438
更新日期:2016-04-02 00:00:00
abstract::Bacterial L-asparaginase has been a universal component of therapies for childhood acute lymphoblastic leukemia since the 1970s. Two principal enzymes derived from Escherichia coli and Erwinia chrysanthemi are the only options clinically approved to date. We recently reported a study of recombinant L-asparaginase (Ans...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.4161/bioe.21710
更新日期:2013-01-01 00:00:00
abstract::The straw can be degraded efficiently into humus by powerful enzymes from microorganisms, resulting in the accelerated circulation of N,P,K and other effective elements in ecological system. We isolated a strain through screening the straw degradation strains from natural humic straw in the low temperature area in nor...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2016.1227143
更新日期:2016-09-02 00:00:00
abstract::The human chromogranin A-derived peptide CGA-N12, which is composed of 12 amino acid residues with the sequence ALQGAKERAHQQ, showed strong antifungal activity and the least hemolytic activity in previous studies. However, synthetic peptides are relatively expensive to produce. Recombinant expression of peptides in th...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2020.1736237
更新日期:2020-12-01 00:00:00
abstract::In recent years there has been great progress with the implementation and utilization of Clustered Regularly Interspaced Palindromic Repeats (CRISPR) and CRISPR-associated protein (Cas) systems in the world of genetic engineering. Many forms of CRISPR-Cas9 have been developed as genome editing tools and techniques and...
journal_title:Bioengineered
pub_type: 杂志文章,评审
doi:10.1080/21655979.2018.1470720
更新日期:2018-01-01 00:00:00
abstract::Protein production through dedicated secretion systems might offer an potential alternative to the conventional cytoplasmical expression. The application of Type 1 secretion systems of Gram-negative bacteria, however, where often not successful in the past for a wide range of proteins. Recently, two studies using the ...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.4161/bioe.20712
更新日期:2012-09-01 00:00:00
abstract::The ascomycete Ophiostoma piceae produces a sterol esterase (OPE) with high affinity toward p-nitrophenol, glycerol, and sterol esters. Recently, this enzyme has been heterologously expressed in the methylotrophic yeast Pichia pastoris under the AOX1 methanol-inducible promoter (PAOX1) using sorbitol as co-susbtrate, ...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.4161/bioe.22818
更新日期:2013-07-01 00:00:00
abstract::The plant-specific RING-domain finger proteins play important roles in plant development and stress responses. We recently identified and functionally characterized a stress-induced gene OsSRFP1 (Oryza sativa Stress-related RING Finger Protein 1) from rice. We showed evidences of the biotechnological potential of the ...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2015.1131368
更新日期:2016-01-01 00:00:00
abstract::We previously presented the cloning, heterologous expression, and characterization of a novel multidomain endoxylanase from Arthrobacter sp. GN16 isolated from the feces of Grus nigricollis. Molecular and biochemical characterization studies indicate that the glycoside hydrolase (GH) family 10 domain at the N-terminus...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2014.1004021
更新日期:2015-01-01 00:00:00
abstract::In this paper, a miniaturized cell suspension concentration monitoring method was investigated. The sensing unit was a carbon screen-printed electrode (CSPE) in serial with a 433MHz vacuum-packaged surface acoustic wave resonator (SAWR). SAWR provided a stable and high operating frequency, which helps to keep the stab...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2015.1119342
更新日期:2015-01-01 00:00:00
abstract::Alkaline pectinase has important applications in the pretreatment of waste water from food processing and in both the fabric and paper industries. In this study, a 2-level factorial design was used to screen significant factors that affect the activity of alkaline pectinase, and the response surface methodology (RSM) ...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2017.1292188
更新日期:2017-09-03 00:00:00
abstract::Tryptophan, an aromatic amino acid, has been widely used in food industry because it participates in the regulation of protein synthesis and metabolic network in vivo. In this study, we obtained a strain named TRP03 by enhancing the tryptophan synthesis pathway, which could accumulate tryptophan at approximately 35 g/...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2019.1592417
更新日期:2019-12-01 00:00:00
abstract::Increasing evidence has shown that the tumor microenvironment (TME) plays an important role in tumor occurrence and development and can also affect patient prognosis. In this study, we screened key prognostic genes in the breast cancer (BC) TME by analyzing the immune and stromal scores of tumor samples to detect diff...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2020.1840731
更新日期:2020-12-01 00:00:00
abstract::Economically feasible production of second-generation biofuels requires efficient co-fermentation of pentose and hexose sugars in lignocellulosic hydrolysates under very harsh conditions. Baker's yeast is an excellent, traditionally used ethanol producer but is naturally not able to utilize pentoses. This is due to th...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.4161/bioe.21444
更新日期:2012-11-01 00:00:00
abstract::Rhizoremediation is one of the most accepted, cost-effective bioremediation techniques focusing on the application of rhizospheric microorganisms in combination with plants for the remediation of organic and inorganic pollutants from the contaminated sites. This work focuses on isolation and identification of metal re...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2020.1728034
更新日期:2020-12-01 00:00:00
abstract::The deep-sea bacterium strain FA13 was isolated from the sediment of the South Atlantic Ocean and identified as Bacillus circulans based on 16S ribosomal DNA sequence. Through liquid fermentation with five media, the cell-free supernatant fermented with ISP2 showed the highest inhibition activities against mycelial gr...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2019.1586055
更新日期:2019-12-01 00:00:00
abstract::Atherosclerosis enables to alter not only the microstructural but also the physical properties of the arterial walls by plaque forming. Few studies so far have been conducted to calculate the isotropic or anisotropic mechanical properties of the healthy and atherosclerotic human coronary arteries. To date there is a p...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2016.1212134
更新日期:2017-03-04 00:00:00
abstract::We demonstrated previously that expression of Rhizomucor miehei lipase (RML) in Pichia pastoris could be significantly increased by addition of gene propeptide, optimized signal peptide codons and manipulation of gene dosage. In this study, effects of various strategies on the protein synthesis and secretion pathways ...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2019.1614422
更新日期:2019-12-01 00:00:00
abstract::Endophytic fungi and bacteria are the most ubiquitous and representative commensal members that have been studied so far in various higher plants. Within colonization and interaction with their host plants, endophytic microbiota are reportedly to modulate not only the host's growth but also holobiont resilience to abi...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2020.1816788
更新日期:2020-12-01 00:00:00
abstract::The edible filamentous fungi are hot candidate for future supply of functional food and feed with e.g. protein, essential amino acids, and compounds with immunostimulant activity. L-carnitine that plays a crucial role in energy metabolism represents a functional compound normally produced by Zygomycetes filamentous fu...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2020.1863618
更新日期:2021-12-01 00:00:00
abstract::N-linked glycosylation is a primary source of heterogeneity associated with recombinant monoclonal antibodies and plays a key role in a myriad of drug properties associated with biological function. The glycosylation profile of recombinant monoclonal antibodies is influenced by an array of cell culture inputs which mu...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2020.1842651
更新日期:2020-12-01 00:00:00