Different construction strategies affected on the physiology of Pichia pastoris strains highly expressed lipase by transcriptional analysis of key genes.

abstract：:As the second most common gynecologic malignant tumors with a high mortality rate, cervical cancer jeopardizes women's life worldwide. The low cure rate in cervical cancer patients is mainly attributed to the lack of effective therapies. One feasible novel strategy is to develop immune-based approaches such as adoptiv...

journal_title：Bioengineered

authors： Zheng Y,Yang Y,Wu S,Zhu Y,Tang X,Liu X

更新日期：2017-07-04 00:00:00

abstract：:The deep-sea bacterium strain FA13 was isolated from the sediment of the South Atlantic Ocean and identified as Bacillus circulans based on 16S ribosomal DNA sequence. Through liquid fermentation with five media, the cell-free supernatant fermented with ISP2 showed the highest inhibition activities against mycelial gr...

journal_title：Bioengineered

authors： Zhou Y,Wang J,Gao X,Wang K,Wang W,Wang Q,Yan P

更新日期：2019-12-01 00:00:00

abstract：:Ulcerative colitis (UC) is a type of refractory and recurrent inflammatory disorder that occurs in colon and rectum. Immune cell infiltration plays a critical role in UC progression; therefore, this study aims to explore potential biomarkers for UC and to analyze characteristics of immune cell infiltration based on th...

journal_title：Bioengineered

authors： Xue G,Hua L,Zhou N,Li J

更新日期：2021-12-01 00:00:00

abstract：:This study is aimed to explore the roles of LINC00528 in myocardial infarction (MI) progression. Quantitative real-time PCR showed that the expression of LINC00528 and COX-2 was upregulated while miR-143-3p expression was down-regulated in post-MI cells. In function assays, LINC00528 suppression promoted post-MI cells...

journal_title：Bioengineered

authors： Liu K,Zhao D,Wang D

更新日期：2020-12-01 00:00:00

abstract：:N-linked glycosylation is a primary source of heterogeneity associated with recombinant monoclonal antibodies and plays a key role in a myriad of drug properties associated with biological function. The glycosylation profile of recombinant monoclonal antibodies is influenced by an array of cell culture inputs which mu...

journal_title：Bioengineered

authors： Schilling M,Feng P,Sosic Z,Traviglia SL

更新日期：2020-12-01 00:00:00

abstract：:Surface plasmon resonance (SPR) sensing is recently emerging as a valuable technique for measuring the binding constants, association and dissociation rate constants, and stoichimetry for a binding interaction kinetics in a number of emerging biological areas. This technique can be applied to the study of immune syste...

journal_title：Bioengineered

authors： Jahanshahi P,Wei Q,Jie Z,Ghomeishi M,Sekaran SD,Mahamd Adikan FR

更新日期：2017-05-04 00:00:00

abstract：:In this study, the conditions of extraction of loquat flowers polyphenolics were optimized through response surface methodology (RSM). Proper extraction conditions were: solid to liquid ratio 1 g per 50 mL and ethanol concentration 50% at 61°C for 9 min. Furthermore, the antioxidant and anti-polyphenol oxidase (PPO) a...

journal_title：Bioengineered

authors： Huang GL,Ma JJ,Sui SY,Wang YN

更新日期：2020-12-01 00:00:00

abstract：:Kidney renal clear cell carcinoma (ccRCC) is a malignant tumor originating from renal tubular epithelium, lncRNAs can regulate the occurrence and development of EMT by targeting EMT transcription factors. We constructed a new survival signature based on EMT-related differentially expressed lncRNAs obtained from The Ca...

journal_title：Bioengineered

authors： Cheng YB,Li S,Zhang YP,Zhu ZH,Zhao N

更新日期：2021-01-31 00:00:00

abstract：:Stromal interaction molecule 1 (STIM1) is a calcium-sensing protein localized in the membrane of the endoplasmic reticulum. The expression of STIM1 has been shown to be closely associated with cell proliferation. The aim of the present study was to investigate the role of STIM1 in the regulation of cancer progression ...

journal_title：Bioengineered

authors： Ge C,Zeng B,Li R,Li Z,Fu Q,Wang W,Wang Z,Dong S,Lai Z,Wang Y,Xue Y,Guo J,Di T,Song X

更新日期：2019-12-01 00:00:00

abstract：:During the last few decades, Streptomycetes have shown to be a very important and adaptable group of bacteria for the production of various beneficial secondary metabolites. These secondary metabolites have been of great interest in academia and the pharmaceutical industries. To date, a vast variety of techniques and ...

journal_title：Bioengineered

authors： Bekker V,Dodd A,Brady D,Rumbold K

更新日期：2014-09-01 00:00:00

abstract：:Tryptophan, an aromatic amino acid, has been widely used in food industry because it participates in the regulation of protein synthesis and metabolic network in vivo. In this study, we obtained a strain named TRP03 by enhancing the tryptophan synthesis pathway, which could accumulate tryptophan at approximately 35 g/...

journal_title：Bioengineered

authors： Du L,Zhang Z,Xu Q,Chen N

更新日期：2019-12-01 00:00:00

abstract：:This study aimed to detect serum miR-203 expression levels in AML and explore its potential clinical significance. Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) was performed to measure the serum miR-203 levels in 134 patients with AML and 70 healthy controls. The results demonstrated that ser...

journal_title：Bioengineered

authors： Guo Y

更新日期：2019-12-01 00:00:00

abstract：:The major drawback of chemical transformations for the production of 2, 5-furan dicarboxylic acid (FDCA) implies the usage of hazardous chemicals, high temperature and high pressure from nonrenewable resources. Alternate to chemical methods, biological methods are promising. Microbial FDCA production is improved throu...

journal_title：Bioengineered

authors： Rajesh RO,Godan TK,Sindhu R,Pandey A,Binod P

更新日期：2020-12-01 00:00:00

abstract：:The ascomycete Ophiostoma piceae produces a sterol esterase (OPE) with high affinity toward p-nitrophenol, glycerol, and sterol esters. Recently, this enzyme has been heterologously expressed in the methylotrophic yeast Pichia pastoris under the AOX1 methanol-inducible promoter (PAOX1) using sorbitol as co-susbtrate, ...

journal_title：Bioengineered

authors： Barba Cedillo V,Prieto A,Martínez MJ

更新日期：2013-07-01 00:00:00

abstract：:The objective is to explore the effect of testosterone on the proliferation and collagen synthesis of neonatal rat cardiac fibroblasts (CF) induced by Angiotensin II (Ang II) and the underlying mechanisms. Derived from neonatal rats, the CFs were divided into 4 groups: the control group, Ang II group, testosterone gro...

journal_title：Bioengineered

authors： Yang X,Wang Y,Yan S,Sun L,Yang G,Li Y,Yu C

更新日期：2017-01-02 00:00:00

abstract：:Pancreatic adenocarcinoma (PAAD) ranks among the most lethal cancers worldwide with high mortality. A marked increase in the level of long non-coding RNA LINC00460 was reported in PAAD patients, in comparison with the healthy controls. However, the underlying mechanisms of the above phenomenon are not yet well underst...

journal_title：Bioengineered

authors： Cheng J,Lou Y,Jiang K

更新日期：2021-12-01 00:00:00

abstract：:The bioengineering tools have significant advantages through less time-consuming and utilized as a promising stage for the production of pharmaceutical bioproducts under the single platform. This review highlighted the advantages and current improvement in the plant, animal and microbial bioengineering tools and outli...

journal_title：Bioengineered

authors： Sarsaiya S,Shi J,Chen J

更新日期：2019-12-01 00:00:00

abstract：:We propose a mechanism of action for the betL* mutation which is based on DNA topology. Removing a single thymine residue from the betL σ(A) promoter's -10 and -35 spacer results in a 'twist'-mediated activation of transcription which accounts for the osmotolerance phenotype observed for strains expressing betL*. ...

journal_title：Bioengineered

authors： Keane SM,Culligan EP,Hoffmann RF,Gahan CG,Hill C,Snelling WJ,Sleator RD

更新日期：2016-04-02 00:00:00

abstract：:Vaccination is the most cost effective strategy for the control and prevention of the plethora of viral diseases affecting poultry production. The major challenge for poultry vaccination is the design of vaccines that will protect against multiple pathogens via a single protective dose, delivered by mass vaccination. ...

journal_title：Bioengineered

authors： Iqbal M

更新日期：2012-07-01 00:00:00

abstract：:Bacterial L-asparaginase has been a universal component of therapies for childhood acute lymphoblastic leukemia since the 1970s. Two principal enzymes derived from Escherichia coli and Erwinia chrysanthemi are the only options clinically approved to date. We recently reported a study of recombinant L-asparaginase (Ans...

journal_title：Bioengineered

authors： Huerta-Saquero A,Evangelista-Martínez Z,Moreno-Enriquez A,Perez-Rueda E

更新日期：2013-01-01 00:00:00

abstract：:Bovine chymosin is an important milk-clotting agent used in the manufacturing of cheeses. Currently, the production of recombinant proteins by genetically modified organisms is widespread, leading to greatly reduced costs. Lactococcus (L.) lactis, the model lactic acid bacterium, was considered a good candidate for he...

journal_title：Bioengineered

authors： Luerce TD,Azevedo MS,LeBlanc JG,Azevedo V,Miyoshi A,Pontes DS

更新日期：2014-01-01 00:00:00

abstract：:CRISPR (Clustered Regularly-Interspaced Short Palindromic Repeats)-Cas9 (CRISPR associated protein 9) has rapidly become the most promising genome editing tool with great potential to revolutionize medicine. Through guidance of a 20 nucleotide RNA (gRNA), CRISPR-Cas9 finds and cuts target protospacer DNA precisely 3 b...

journal_title：Bioengineered

authors： Zhang JH,Adikaram P,Pandey M,Genis A,Simonds WF

更新日期：2016-04-01 00:00:00

abstract：:The edible filamentous fungi are hot candidate for future supply of functional food and feed with e.g. protein, essential amino acids, and compounds with immunostimulant activity. L-carnitine that plays a crucial role in energy metabolism represents a functional compound normally produced by Zygomycetes filamentous fu...

journal_title：Bioengineered

authors： Rousta N,Ferreira JA,Taherzadeh MJ

更新日期：2021-12-01 00:00:00

abstract：:The detecting mechanism of carbon nanotubes gas sensor based on multi-stable stochastic resonance (MSR) model was studied in this paper. A numerically stimulating model based on MSR was established. And gas-ionizing experiment by adding electronic white noise to induce 1.65 MHz periodic component in the carbon nanotub...

journal_title：Bioengineered

authors： Jingyi Z

更新日期：2015-01-01 00:00:00

abstract：:Organ transplantation can offer a curative option for patients with end stage organ failure. Unfortunately the treatment is severely limited by the availability of donor organs. Organ bioengineering could provide a solution to the worldwide critical organ shortage. The majority of protocols to date have employed the u...

journal_title：Bioengineered

authors： Welman T,Michel S,Segaren N,Shanmugarajah K

更新日期：2015-01-01 00:00:00

abstract：:Illicium verum (badiane or star anise), Crataegus oxyacantha ssp monogyna (hawthorn) and Allium cepa (onion), have traditionnally been used as medicinal plants in Algeria. This study showed that the outer layer of onion is rich in flavonols with contents of 103 ± 7.90 µg/g DW (red variety) and 17.3 ± 0.69 µg/gDW (whit...

journal_title：Bioengineered

authors： Benmalek Y,Yahia OA,Belkebir A,Fardeau ML

更新日期：2013-07-01 00:00:00

abstract：:Circular RNA (circRNA) circ-LRP6 was recently proven to be a pivotal player in various human diseases. Nevertheless, its role in esophageal squamous cell cancer (ESCC) remains unknown. In the current study, we investigated the expression level, biological function and mechanism of circ-LRP6 in ESCC. Circ-LRP6 was sign...

journal_title：Bioengineered

authors： Wang J,Zhu W,Tao G,Wang W

更新日期：2020-12-01 00:00:00

abstract：:Extraction of sugar is the rate-limiting step in converting unpretreated biomass into value-added products through microbial fermentation. Both anaerobic fungi and anaerobic bacteria have evolved to produce large multi-cellulase complexes referred to as cellulosomes, which are powerful machines for biomass deconstruct...

journal_title：Bioengineered

authors： Gilmore SP,Henske JK,O'Malley MA

更新日期：2015-01-01 00:00:00

abstract：:The influenza A virus is a critical public health problem that causes epidemics and pandemics, and occurs widely all over the world. Various vaccines against the virus have not provided a solution to the problem. Different approaches, particularly M2e peptide-based vaccines, are available for developing universal vacc...

journal_title：Bioengineered

authors： Kilinc YB,Akdeste ZM,Koc RC,Bagirova M,Allahverdiyev A

更新日期：2014-01-01 00:00:00

abstract：:Epstein-Barr virus (EBV) is an oncogenic virus that infects more than 90% of the world's population. The proteins and miRNAs encoded by EBV are involved in multiple human malignancies. Recently R-resistance RNA-seq demonstrated that EBV-encoded circular RNAs. The current research aims to explore their functions in EBV...

journal_title：Bioengineered

authors： Qiao Y,Zhao X,Liu J,Yang W

更新日期：2019-12-01 00:00:00