Driving biomass breakdown through engineered cellulosomes.

Abstract:

:Extraction of sugar is the rate-limiting step in converting unpretreated biomass into value-added products through microbial fermentation. Both anaerobic fungi and anaerobic bacteria have evolved to produce large multi-cellulase complexes referred to as cellulosomes, which are powerful machines for biomass deconstruction. Characterization of bacterial cellulosomes has inspired synthetic "designer" cellulosomes, consisting of parts discovered from the native system that have proven useful for cellulose depolymerization. By contrast, the multi-cellulase complexes produced by anaerobic fungi are much more poorly understood, and to date their composition, architecture, and enzyme tethering mechanism remain unknown and heavily debated. Here, we compare current knowledge pertaining to the cellulosomes produced by both bacteria and fungi, including their application to synthetic enzyme-tethered systems for tunneled biocatalysis. We highlight gaps in knowledge and opportunities for discovery, especially pertaining to the potential of fungal cellulosome-inspired systems.

journal_name

Bioengineered

journal_title

Bioengineered

authors

Gilmore SP,Henske JK,O'Malley MA

doi

10.1080/21655979.2015.1060379

subject

Has Abstract

pub_date

2015-01-01 00:00:00

pages

204-8

issue

4

eissn

2165-5979

issn

2165-5987

journal_volume

6

pub_type

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