Abstract:
:Mammalian collagen has been widely used as a biomedical material. Nevertheless, there are still concerns about the variability between preparations, particularly with the possibility that the products may transmit animal-based diseases. Many groups have examined the possible application of bioengineered mammalian collagens. However, translating laboratory studies into large-scale manufacturing has often proved difficult, although certain yeast and plant systems seem effective. Production of full-length mammalian collagens, with the required secondary modification to give proline hydroxylation, has proved difficult in E. coli. However, recently, a new group of collagens, which have the characteristic triple helical structure of collagen, has been identified in bacteria. These proteins are stable without the need for hydroxyproline and are able to be produced and purified from E. coli in high yield. Initial studies indicate that they would be suitable for biomedical applications.
journal_name
Bioengineeredjournal_title
Bioengineeredauthors
Ramshaw JA,Werkmeister JA,Dumsday GJdoi
10.4161/bioe.28791subject
Has Abstractpub_date
2014-07-01 00:00:00pages
227-33issue
4eissn
2165-5979issn
2165-5987pii
28791journal_volume
5pub_type
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