Separation and purification of ginsenoside Re from ginseng bud by selective adsorption of active carbon and preparative high-performance liquid chromatography.

Abstract:

:Ginsenoside Re (G-Re) is a major ingredient of the ginseng bud. A novel and rapid method to isolate and purify G-Re from ginseng buds was established. The procedure involves solvent extraction of ginseng bud powder, then pre-purification with an active carbon column and purification by preparative HPLC (prep-HPLC). Active carbon, which can selectively adsorb other kinds of ginsenosides except G-Re, was used as a new media to pre-purify G-Re in this study. In addition, we describe the development and optimisation of prep-HPLC parameters for G-Re purification. Compared to other types of high-purity G-Re preparation methods, this method is efficient, economical, waste-conscious and has the potential to maximise.

journal_name

Nat Prod Res

journal_title

Natural product research

authors

Kuang P,Wang G,Yuan Q,Liang H

doi

10.1080/14786419.2010.540577

subject

Has Abstract

pub_date

2012-01-01 00:00:00

pages

286-90

issue

3

eissn

1478-6419

issn

1478-6427

journal_volume

26

pub_type

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