Rapid characterisation of flavonoids from Sophora alopecuroides L. by HPLC/DAD/ESI-MSn.

Abstract:

:An HPLC/DAD/ESI-MSn method was established to characterise flavonoids in the seeds of Sophora alopecuroides L. All the flavonoids exhibited abundant [M-H]- ions, which triggered data-dependent multistage mass spectrometry fragmentations. Characteristic UV spectra were used to confirm flavonoid subtypes. In total, 22 flavonoids and two phenolic compounds were detected and identified. Five of them were identified by comparing with reference standards, and the others were characterised based on the retention behaviour, multistage fragmentation feature and UV absorption. A total of 19 compounds were reported from S. alopecuroides for the first time. This method accomplished rapid profiling of flavonoid constituents in S. alopecuroides L.

journal_name

Nat Prod Res

journal_title

Natural product research

authors

Yang WZ,Ye G,Meng AH,Sabir G,Qiao X,Guo DA,Ye M

doi

10.1080/14786419.2012.688052

subject

Has Abstract

pub_date

2013-03-01 00:00:00

pages

323-30

issue

4-5

eissn

1478-6419

issn

1478-6427

journal_volume

27

pub_type

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