Abstract：
:The ubiquitin receptors Rad23 and Dsk2 deliver polyubiquitylated substrates to the proteasome for destruction. The C-terminal ubiquitin-associated (UBA) domain of Rad23 functions as a cis-acting stabilization signal that protects this protein from proteasomal degradation. Here, we provide evidence that the C-terminal UBA domains guard ubiquitin receptors from destruction by preventing initiation of degradation at the proteasome. We show that introduction of unstructured polypeptides that are sufficiently long to function as initiation sites for degradation abrogates the protective effect of UBA domains. Vice versa, degradation of substrates that contain an unstructured extension can be attenuated by the introduction of C-terminal UBA domains. Our study gains insight into the molecular mechanism responsible for the protective effect of UBA domains and explains how ubiquitin receptors can shuttle substrates to the proteasome without themselves becoming subject to proteasomal degradation.
journal_name
Nat Communjournal_title
Nature communicationsauthors
Heinen C,Acs K,Hoogstraten D,Dantuma NPdoi
10.1038/ncomms1179subject
Has Abstractpub_date
2011-02-08 00:00:00pages
191issn
2041-1723pii
ncomms1179journal_volume
2pub_type
杂志文章abstract：:Studies on vesicle formation by the Coat Protein I (COPI) complex have contributed to a basic understanding of how vesicular transport is initiated. Phosphatidic acid (PA) and diacylglycerol (DAG) have been found previously to be required for the fission stage of COPI vesicle formation. Here, we find that PA with vary...
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pub_type： 杂志文章
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pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Nature communications
pub_type： 杂志文章
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pub_type： 杂志文章
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pub_type： 杂志文章
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更新日期：2020-02-25 00:00:00
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pub_type： 杂志文章
doi：10.1038/ncomms8219
更新日期：2015-05-26 00:00:00
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journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/ncomms13808
更新日期：2016-12-15 00:00:00
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pub_type： 杂志文章
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更新日期：2014-11-26 00:00:00
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更新日期：2017-11-27 00:00:00
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更新日期：2015-04-22 00:00:00
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pub_type： 杂志文章
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更新日期：2017-12-19 00:00:00
