Abstract:
:Chronic myeloid leukaemia (CML) is caused by a defined genetic abnormality that generates BCR-ABL, a constitutively active tyrosine kinase. It is widely believed that BCR-ABL activates Akt signalling that suppresses the forkhead O transcription factors (FOXO), supporting the proliferation or inhibiting the apoptosis of CML cells. Although the use of the tyrosine kinase inhibitor imatinib is a breakthrough for CML therapy, imatinib does not deplete the leukaemia-initiating cells (LICs) that drive the recurrence of CML. Here, using a syngeneic transplantation system and a CML-like myeloproliferative disease mouse model, we show that Foxo3a has an essential role in the maintenance of CML LICs. We find that cells with nuclear localization of Foxo3a and decreased Akt phosphorylation are enriched in the LIC population. Serial transplantation of LICs generated from Foxo3a(+/+) and Foxo3a(-/-) mice shows that the ability of LICs to cause disease is significantly decreased by Foxo3a deficiency. Furthermore, we find that TGF-beta is a critical regulator of Akt activation in LICs and controls Foxo3a localization. A combination of TGF-beta inhibition, Foxo3a deficiency and imatinib treatment led to efficient depletion of CML in vivo. Furthermore, the treatment of human CML LICs with a TGF-beta inhibitor impaired their colony-forming ability in vitro. Our results demonstrate a critical role for the TGF-beta-FOXO pathway in the maintenance of LICs, and strengthen our understanding of the mechanisms that specifically maintain CML LICs in vivo.
journal_name
Naturejournal_title
Natureauthors
Naka K,Hoshii T,Muraguchi T,Tadokoro Y,Ooshio T,Kondo Y,Nakao S,Motoyama N,Hirao Adoi
10.1038/nature08734subject
Has Abstractpub_date
2010-02-04 00:00:00pages
676-80issue
7281eissn
0028-0836issn
1476-4687pii
nature08734journal_volume
463pub_type
杂志文章相关文献
NATURE文献大全abstract::Subcutaneous (s.c.) administration of Arg-vasopressin (AVP) prolongs retention of a learned behaviour and elevates arterial blood pressure. Intracerebroventricular (i.c.v.) injection of a thousandfold lower dose of AVP than needed with s.c. injection produces the same behavioural effect, suggesting that AVP acts on th...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/301608a0
更新日期:1983-02-17 00:00:00
abstract::The first event in the initiation of an immune response is the capture and presentation of antigen to T cells. Such presentation involves two distinct steps: (1) display of the antigen, which requires uptake, processing and re-expression of the antigen in association with MHC molecules on the presenting cell surface; ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/334253a0
更新日期:1988-07-21 00:00:00
abstract::The angle between the spin of a star and the orbital planes of its planets traces the history of the planetary system. Exoplanets orbiting close to cool stars are expected to be on circular, aligned orbits because of strong tidal interactions with the stellar convective envelope. Spin-orbit alignment can be measured w...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature24677
更新日期:2018-01-25 00:00:00
abstract::Several of the thousands of human long non-coding RNAs (lncRNAs) have been functionally characterized; however, potential roles for lncRNAs in somatic tissue differentiation remain poorly understood. Here we show that a 3.7-kilobase lncRNA, terminal differentiation-induced ncRNA (TINCR), controls human epidermal diffe...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature11661
更新日期:2013-01-10 00:00:00
abstract::Understanding how ecological communities are organized and how they change through time is critical to predicting the effects of climate change. Recent work documenting the co-occurrence structure of modern communities found that most significant species pairs co-occur less frequently than would be expected by chance....
journal_title:Nature
pub_type: 历史文章,杂志文章
doi:10.1038/nature16447
更新日期:2016-01-07 00:00:00
abstract::There is compelling evidence that sleep contributes to the long-term consolidation of new memories. This function of sleep has been linked to slow (<1 Hz) potential oscillations, which predominantly arise from the prefrontal neocortex and characterize slow wave sleep. However, oscillations in brain potentials are comm...
journal_title:Nature
pub_type: 临床试验,杂志文章
doi:10.1038/nature05278
更新日期:2006-11-30 00:00:00
abstract::Oncogenic mutations in the serine/threonine kinase B-RAF (also known as BRAF) are found in 50-70% of malignant melanomas. Pre-clinical studies have demonstrated that the B-RAF(V600E) mutation predicts a dependency on the mitogen-activated protein kinase (MAPK) signalling cascade in melanoma-an observation that has bee...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature09627
更新日期:2010-12-16 00:00:00
abstract::For life to be sustained, mistakes in DNA repair must be tolerated when damage obscures the genetic information. In bacteria such as Escherichia coli, DNA damage elicits the well regulated 'SOS response'. For the extreme case of damage that cannot be repaired by conventional enzymes, there are proteins that allow the ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/380727a0
更新日期:1996-04-25 00:00:00
abstract::TATA-binding protein (TBP) binds the minor groove of the TATA element with the DNA bent 80 degrees towards the major groove. A constrained minicircle strategy has been used to test the effect of DNA topology on the affinity of TBP for the TATA element. We report here that TBP bound to DNA which was slightly pre-bent t...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/373724a0
更新日期:1995-02-23 00:00:00
abstract::The coupling of ATP hydrolysis to electron transfer by the enzyme nitrogenase during biological nitrogen fixation is an important example of a nucleotide-dependent transduction mechanism. The crystal structure has been determined for the complex between the Fe-protein and MoFe-protein components of nitrogenase stabili...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/387370a0
更新日期:1997-05-22 00:00:00
abstract::(S)-2-hydroxypropylphosphonate ((S)-2-HPP) epoxidase (HppE) is a mononuclear non-haem-iron-dependent enzyme responsible for the final step in the biosynthesis of the clinically useful antibiotic fosfomycin. Enzymes of this class typically catalyse oxygenation reactions that proceed via the formation of substrate radic...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature11998
更新日期:2013-04-04 00:00:00
abstract::Defects in the architecture or integrity of the nuclear envelope are associated with a variety of human diseases1. Micronuclei, one common nuclear aberration, are an origin for chromothripsis2, a catastrophic mutational process that is commonly observed in cancer3-5. Chromothripsis occurs after micronuclei spontaneous...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/s41586-018-0534-z
更新日期:2018-09-01 00:00:00
abstract::The three-dimensional structure of the ligand-binding region of human E-selectin has been determined at 2.0 A resolution. The structure reveals limited contact between the two domains and a coordination of Ca2+ not predicted from other C-type lectins. Structure/function analysis indicates a defined region and specific...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/367532a0
更新日期:1994-02-10 00:00:00
abstract::Cysticercosis caused by larval tapeworms is a major public health problem and a cause of substantial economic losses in the farm-animal industries. Taenia ovis in sheep is a particularly important example. Immunity to reinfection with the larvae has a central role in regulating natural transmission of the parasites, a...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/338585a0
更新日期:1989-04-13 00:00:00
abstract::The travelling salesman problem is a classical problem in the field of combinatorial optimization, concerned with efficient methods for maximizing or minimizing a function of many independent variables. Given the positions of N cities, which in the simplest case lie in the plane, what is the shortest closed tour in wh...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/326689a0
更新日期:1987-04-16 00:00:00
abstract::Little is known about the biochemical or functional nature of the proteins encoded by the bcl-2 gene, which undergoes chromosomal translocation in approximately 85% of follicular lymphoma, 20% of diffuse large cell lymphoma and 10% of chronic lymphocytic leukaemia of B cells. Translocation of bcl-2 sequences from chro...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/342195a0
更新日期:1989-11-09 00:00:00
abstract::Do biological motors move with regular steps? To address this question, we constructed instrumentation with the spatial and temporal sensitivity to resolve movement on a molecular scale. We deposited silica beads carrying single molecules of the motor protein kinesin on microtubules using optical tweezers and analysed...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/365721a0
更新日期:1993-10-21 00:00:00
abstract::The largest products of magmatic activity on Earth, the great bodies of granite and their corresponding large eruptions, have a dual nature: homogeneity at the large scale and spatial and temporal heterogeneity at the small scale. This duality calls for a mechanism that selectively removes the large-scale heterogeneit...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature09799
更新日期:2011-03-10 00:00:00
abstract::Secreted signalling molecules of the Hedgehog (Hh) family have many essential patterning roles during development of diverse organisms including Drosophila and humans. Although Hedgehog proteins most commonly affect cell fate, they can also stimulate cell proliferation. In humans several distinctive cancers, including...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/35069099
更新日期:2001-03-29 00:00:00
abstract::The structure of an N-terminal fragment of CD4 has been determined to 2.4 A resolution. It has two tightly abutting domains connected by a continuous beta strand. Both have the immunoglobulin fold, but domain 2 has a truncated beta barrel and a non-standard disulphide bond. The binding sites for monoclonal antibodies,...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/348411a0
更新日期:1990-11-29 00:00:00
abstract::Among the pathological effects in man following infection with Mycoplasma pneumoniae is a transient autoimmune disorder characterized by the presence of high-titre erythrocyte autoantibodies (cold agglutinins). These autoantibodies are usually directed against the carbohydrate antigen termed I (ref. 3) which consists ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/307560a0
更新日期:1984-02-09 00:00:00
abstract::Countless millions of people have died from tuberculosis, a chronic infectious disease caused by the tubercle bacillus. The complete genome sequence of the best-characterized strain of Mycobacterium tuberculosis, H37Rv, has been determined and analysed in order to improve our understanding of the biology of this slow-...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/31159
更新日期:1998-06-11 00:00:00
abstract::A variety of microbial communities and their genes (the microbiome) exist throughout the human body, with fundamental roles in human health and disease. The National Institutes of Health (NIH)-funded Human Microbiome Project Consortium has established a population-scale framework to develop metagenomic protocols, resu...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature11209
更新日期:2012-06-13 00:00:00
abstract::Early observations indicated that the Earth's Van Allen radiation belts could be separated into an inner zone dominated by high-energy protons and an outer zone dominated by high-energy electrons. Subsequent studies showed that electrons of moderate energy (less than about one megaelectronvolt) often populate both zon...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature13956
更新日期:2014-11-27 00:00:00
abstract::Haematopoietic stem cells (HSCs) sustain blood production throughout life. HSCs are capable of extensive proliferative expansion, as a single HSC may reconstitute lethally irradiated hosts. In steady-state, HSCs remain largely quiescent and self-renew at a constant low rate, forestalling their exhaustion during adult ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature02994
更新日期:2004-10-21 00:00:00
abstract::Targeted gene disruption in the mouse shows that the Sonic hedgehog (Shh) gene plays a critical role in patterning of vertebrate embryonic tissues, including the brain and spinal cord, the axial skeleton and the limbs. Early defects are observed in the establishment or maintenance of midline structures, such as the no...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/383407a0
更新日期:1996-10-03 00:00:00
abstract::The recently emerged fields of metamaterials and transformation optics promise a family of exciting applications such as invisibility, optical imaging with deeply subwavelength resolution and nanophotonics with the potential for much faster information processing. The possibility of creating optical negative-index met...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature09278
更新日期:2010-08-05 00:00:00
abstract::Mutations in the transcription factor FOXA1 define a unique subset of prostate cancers but the functional consequences of these mutations and whether they confer gain or loss of function is unknown1-9. Here, by annotating the landscape of FOXA1 mutations from 3,086 human prostate cancers, we define two hotspots in the...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/s41586-019-1318-9
更新日期:2019-07-01 00:00:00
abstract::The detection of electron spins associated with single defects in solids is a critical operation for a range of quantum information and measurement applications under development. So far, it has been accomplished for only two defect centres in crystalline solids: phosphorus dopants in silicon, for which electrical rea...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature12081
更新日期:2013-05-02 00:00:00
abstract::Indirect reciprocity is a key mechanism for the evolution of human cooperation. Our behaviour towards other people depends not only on what they have done to us but also on what they have done to others. Indirect reciprocity works through reputation. The standard model of indirect reciprocity offers a binary choice: p...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature07601
更新日期:2009-01-01 00:00:00