Direct observation of kinesin stepping by optical trapping interferometry.

Abstract:

:Do biological motors move with regular steps? To address this question, we constructed instrumentation with the spatial and temporal sensitivity to resolve movement on a molecular scale. We deposited silica beads carrying single molecules of the motor protein kinesin on microtubules using optical tweezers and analysed their motion under controlled loads by interferometry. We find that kinesin moves with 8-nm steps.

journal_name

Nature

journal_title

Nature

authors

Svoboda K,Schmidt CF,Schnapp BJ,Block SM

doi

10.1038/365721a0

subject

Has Abstract

pub_date

1993-10-21 00:00:00

pages

721-7

issue

6448

eissn

0028-0836

issn

1476-4687

journal_volume

365

pub_type

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