Revisiting the COP9 signalosome as a transcriptional regulator.

abstract：:The Polycomb group (PcG) of proteins conveys epigenetic inheritance of repressed transcriptional states. In Drosophila, the Polycomb repressive complex 1 (PRC1) maintains the silent state by inhibiting the transcription machinery and chromatin remodelling at core promoters. Using immunoprecipitation of in vivo formald...

journal_title：EMBO reports

authors： Breiling A,O'Neill LP,D'Eliseo D,Turner BM,Orlando V

更新日期：2004-10-01 00:00:00

abstract：:The hypoxic response in humans is mediated by the hypoxia-inducible transcription factor (HIF), for which prolyl hydroxylases (PHDs) act as oxygen-sensing components. The evolutionary origins of the HIF system have been previously unclear. We demonstrate a functional HIF system in the simplest animal, Trichoplax adhae...

journal_title：EMBO reports

authors： Loenarz C,Coleman ML,Boleininger A,Schierwater B,Holland PW,Ratcliffe PJ,Schofield CJ

更新日期：2011-01-01 00:00:00

abstract：:The active and inactive X chromosomes have distinct epigenetic marks in somatic nuclei, which undergo reprogramming after transplantation into oocytes. We show that, despite the disappearance of Xist RNA coating in 30 min, the epigenetic memory of the inactive X persists with the precocious appearance of histone H3 tr...

journal_title：EMBO reports

authors： Bao S,Miyoshi N,Okamoto I,Jenuwein T,Heard E,Azim Surani M

更新日期：2005-08-01 00:00:00

abstract：:The accumulation of misfolded proteins in the endoplasmic reticulum (ER) activates the Unfolded Protein Response (UPR(ER)) to restore ER homeostasis. The AAA(+) ATPase p97/CDC-48 plays key roles in ER stress by promoting both ER protein degradation and transcription of UPR(ER) genes. Although the mechanisms associated...

journal_title：EMBO reports

authors： Marza E,Taouji S,Barroso K,Raymond AA,Guignard L,Bonneu M,Pallares-Lupon N,Dupuy JW,Fernandez-Zapico ME,Rosenbaum J,Palladino F,Dupuy D,Chevet E

更新日期：2015-03-01 00:00:00

abstract：:Elongation of the telomeric overhang by telomerase is counteracted by synthesis of the complementary strand by the CST complex, CTC1(Cdc13)/Stn1/Ten1. Interaction of budding yeast Stn1 with overhang-binding Cdc13 is increased by Cdc13 SUMOylation. Human and fission yeast CST instead interact with overhang-binding TPP1...

journal_title：EMBO reports

authors： Garg M,Gurung RL,Mansoubi S,Ahmed JO,Davé A,Watts FZ,Bianchi A

更新日期：2014-08-01 00:00:00

abstract：:Extracellular vesicles (EVs) have been shown to carry microbial components and function in the host defense against infections. In this study, we demonstrate that Mycobacterium tuberculosis (M.tb) RNA is delivered into macrophage-derived EVs through an M.tb SecA2-dependent pathway and that EVs released from M.tb-infec...

journal_title：EMBO reports

authors： Cheng Y,Schorey JS

更新日期：2019-03-01 00:00:00

abstract：:The mitochondrial matrix protease CLPP plays a central role in the activation of the mitochondrial unfolded protein response (UPR(mt)) in Caenorhabditis elegans Far less is known about mammalian UPR(mt) signaling, although similar roles were assumed for central players, including CLPP To better understand the mammalia...

journal_title：EMBO reports

authors： Seiferling D,Szczepanowska K,Becker C,Senft K,Hermans S,Maiti P,König T,Kukat A,Trifunovic A

更新日期：2016-07-01 00:00:00

abstract：:The mechanism by which YopP simultaneously inhibits mitogen-activated protein kinase (MAPK) and nuclear factor-kappaB pathways has been elusive. Ectopic expression of YopP inhibits the activity and ubiquitination of a complex consisting of overexpressed TGF-beta-activated kinase 1 (TAK1) and its subunit TAK1-binding p...

journal_title：EMBO reports

authors： Thiefes A,Wolf A,Doerrie A,Grassl GA,Matsumoto K,Autenrieth I,Bohn E,Sakurai H,Niedenthal R,Resch K,Kracht M

更新日期：2006-08-01 00:00:00

abstract：:The human XAB1/MBDin GTPase and its close homologues form one of the ten phylogenetically distinct families of the SIMIBI (after signal recognition particle, MinD and BioD) class of phosphate-binding loop NTPases. The genomic context and the partners identified for the archaeal and eukaryotic homologues indicate that ...

journal_title：EMBO reports

authors： Gras S,Chaumont V,Fernandez B,Carpentier P,Charrier-Savournin F,Schmitt S,Pineau C,Flament D,Hecker A,Forterre P,Armengaud J,Housset D

更新日期：2007-06-01 00:00:00

abstract：:Short interfering RNAs (siRNAs) are short (21-23 nt) double-stranded RNAs that direct the sequence-specific degradation of corresponding mRNAs, resulting in suppression of gene activity. siRNAs are powerful tools for gene functional analysis in mammals. Chemically synthesized siRNAs permit transient gene repression bu...

journal_title：EMBO reports

authors： Fritsch L,Martinez LA,Sekhri R,Naguibneva I,Gérard M,Vandromme M,Schaeffer L,Harel-Bellan A

更新日期：2004-02-01 00:00:00

abstract：:Anopheles gambiae, the major vector of human malaria parasite, is an important insect model to study vector-parasite interactions. Here, we developed a simple in vivo double-stranded RNA (dsRNA) knockout approach to determine the function of the mosquito antimicrobial peptide gene Defensin. We injected dsRNA into adul...

journal_title：EMBO reports

authors： Blandin S,Moita LF,Köcher T,Wilm M,Kafatos FC,Levashina EA

更新日期：2002-09-01 00:00:00

abstract：:Alternative splicing (AS) is a major source of transcriptome diversity. Long noncoding RNAs (lncRNAs) have emerged as regulators of AS through different molecular mechanisms. In Arabidopsis thaliana, the AS regulators NSRs interact with the ALTERNATIVE SPLICING COMPETITOR (ASCO) lncRNA. Here, we analyze the effect of ...

journal_title：EMBO reports

authors： Rigo R,Bazin J,Romero-Barrios N,Moison M,Lucero L,Christ A,Benhamed M,Blein T,Huguet S,Charon C,Crespi M,Ariel F

更新日期：2020-05-06 00:00:00

abstract：:Mitochondrial respiration generates an electrochemical proton gradient across the mitochondrial inner membrane called protonmotive force (PMF) to drive diverse functions and synthesize ATP. Current techniques to manipulate the PMF are limited to its dissipation; yet, there is no precise and reversible method to increa...

journal_title：EMBO reports

authors： Berry BJ,Trewin AJ,Milliken AS,Baldzizhar A,Amitrano AM,Lim Y,Kim M,Wojtovich AP

更新日期：2020-04-03 00:00:00

abstract：:The impact of the mitochondrial permeability transition (MPT) on cellular physiology is well characterized. In contrast, the composition and mode of action of the permeability transition pore complex (PTPC), the supramolecular entity that initiates MPT, remain to be elucidated. Specifically, the precise contribution o...

journal_title：EMBO reports

authors： Bonora M,Morganti C,Morciano G,Pedriali G,Lebiedzinska-Arciszewska M,Aquila G,Giorgi C,Rizzo P,Campo G,Ferrari R,Kroemer G,Wieckowski MR,Galluzzi L,Pinton P

更新日期：2017-07-01 00:00:00

abstract：:Stat1 (signal transducer and activator of transcription 1) regulates transcription in response to the type I interferons IFN-alpha and IFN-beta, either in its dimerized form or as a subunit of the interferon-stimulated gene factor 3 (Isgf3) complex (consisting of Stat1, Stat2 and interferon-regulating factor 9). Full-...

journal_title：EMBO reports

authors： Pilz A,Ramsauer K,Heidari H,Leitges M,Kovarik P,Decker T

更新日期：2003-04-01 00:00:00

abstract：:Antibiotic resistance is a grave threat for public health. Understanding the mechanisms of resistance could lead to new drugs and therapeutic strategies against resistant pathogens. ...

journal_title：EMBO reports

authors： Ouellette M,Bhattacharya A

更新日期：2020-04-03 00:00:00

abstract：:X inactivation is the mechanism by which mammals adjust the genetic imbalance that arises from the different numbers of gene-rich X-chromosomes between the sexes. The dosage difference between XX females and XY males is functionally equalized by silencing one of the two X chromosomes in females. This dosage-compensati...

journal_title：EMBO reports

authors： Ng K,Pullirsch D,Leeb M,Wutz A

更新日期：2007-01-01 00:00:00

abstract：:A number of gene silencing phenomena that inactivate genes at the post-transcriptional level have been identified. Due to its potential for studying gene function, post-transcriptional gene silencing (PTGS) has become an intense area of research. In this review we describe the different means of inducing PTGS and disc...

journal_title：EMBO reports

authors： Chicas A,Macino G

更新日期：2001-11-01 00:00:00

abstract：:Regulated intramembrane proteolysis of the amyloid precursor-protein (APP) produces both a characterstic amyloid-β peptide that contributes to neuritic plaque formation and neurodegeneration in Alzheimer disease and a small APP intracellular domain (AICD) that transcriptionally activates genes implicated in Alzheimer ...

journal_title：EMBO reports

authors： Xu X,Zhou H,Boyer TG

更新日期：2011-03-01 00:00:00

abstract：:Autophagosome formation is promoted by the PI3 kinase complex and negatively regulated by myotubularin phosphatases, indicating that regulation of local phosphatidylinositol 3-phosphate (PtdIns3P) levels is important for this early phase of autophagy. Here, we show that the Caenorhabditis elegans myotubularin phosphat...

journal_title：EMBO reports

authors： Wu Y,Cheng S,Zhao H,Zou W,Yoshina S,Mitani S,Zhang H,Wang X

更新日期：2014-09-01 00:00:00

abstract：:Central to chemotaxis is the molecular mechanism by which cells exhibit directed movement in shallow gradients of a chemoattractant. We used Dictyostelium mutants to investigate the minimal requirements for chemotaxis, and identified a basal signalling module providing activation of Ras at the leading edge, which is s...

journal_title：EMBO reports

authors： Kortholt A,Kataria R,Keizer-Gunnink I,Van Egmond WN,Khanna A,Van Haastert PJ

更新日期：2011-12-01 00:00:00

abstract：:One limitation for the study of chromosomal fragile sites is that they must be studied on metaphase spreads, after the breakage. We show here that bacterial lac operator (lacO) repeats are prone to spontaneous breakage, which when combined with a fluorescent lac repressor (lacR) has allowed us to track a fragile site ...

journal_title：EMBO reports

authors： Jacome A,Fernandez-Capetillo O

更新日期：2011-09-30 00:00:00

abstract：:Trypanosomes use RNA editing to produce most functional mitochondrial messenger RNA. Precise insertion and deletion of hundreds of uridines is necessary to make full-length cytochrome c oxidase III (COXIII) mRNA. We show that COXIII mRNA can be alternatively edited by a mechanism using an alternative guide RNA to make...

journal_title：EMBO reports

authors： Ochsenreiter T,Hajduk SL

更新日期：2006-11-01 00:00:00

abstract：:The cytosolic pathogen sensor RIG-I is activated by RNAs with exposed 5'-triphosphate (5'-ppp) and terminal double-stranded structures, such as those that are generated during viral infection. RIG-I has been shown to translocate on dsRNA in an ATP-dependent manner. However, the precise role of the ATPase activity in R...

journal_title：EMBO reports

authors： Patel JR,Jain A,Chou YY,Baum A,Ha T,García-Sastre A

更新日期：2013-09-01 00:00:00

abstract：:RNA modifications have recently emerged as an important layer of gene regulation. N6-methyladenosine (m6 A) is the most prominent modification on eukaryotic messenger RNA and has also been found on noncoding RNA, including ribosomal and small nuclear RNA. Recently, several m6 A methyltransferases were identified, unco...

journal_title：EMBO reports

authors： Leismann J,Spagnuolo M,Pradhan M,Wacheul L,Vu MA,Musheev M,Mier P,Andrade-Navarro MA,Graille M,Niehrs C,Lafontaine DL,Roignant JY

更新日期：2020-07-03 00:00:00

abstract：:Somatic hypermutation of immunoglobulin variable genes, which increases antibody diversity, is initiated by the activation-induced cytosine deaminase (AID) protein. The current DNA-deamination model posits that AID deaminates cytosine to uracil in DNA, and that mutations are generated by DNA polymerases during replica...

journal_title：EMBO reports

authors： Seki M,Gearhart PJ,Wood RD

更新日期：2005-12-01 00:00:00

abstract：:MicroRNAs (miRNAs) originate from stemloop-forming precursor RNAs found in longer primary transcripts that often contain introns. We show that in plants, those introns, when located 3' of the stemloop, can promote mature miRNA accumulation, through a mechanism that likely operates at the level of miRNA processing or s...

journal_title：EMBO reports

authors： Schwab R,Speth C,Laubinger S,Voinnet O

更新日期：2013-07-01 00:00:00

abstract：:Intercellular communication orchestrates effective immune responses against disease-causing agents. Extracellular vesicles (EVs) are potent mediators of cell-cell communication. EVs carry bioactive molecules, including microRNAs, which modulate gene expression and function in the recipient cell. Here, we show that for...

journal_title：EMBO reports

authors： Fernández-Messina L,Rodríguez-Galán A,de Yébenes VG,Gutiérrez-Vázquez C,Tenreiro S,Seabra MC,Ramiro AR,Sánchez-Madrid F

更新日期：2020-04-03 00:00:00

abstract：:In Escherichia coli cells, the origin of chromosomal replication is temporarily inactivated after initiation has occurred. Origin sequestration is the first line of defence against over-initiation, providing a time window during which the initiation potential can be reduced by: (i) titration of DnaA proteins to newly ...

journal_title：EMBO reports

authors： Boye E,Løbner-Olesen A,Skarstad K

更新日期：2000-12-01 00:00:00

abstract：:We have identified a novel component, Helicase89B, that is required for the inducible antimicrobial response in Drosophila larvae by means of a P-element insertional genetic screen. Helicase89B belongs to the Mot1p/BTAF1 subfamily of SNF2-like ATPases. This subfamily can interact with TATA-binding proteins, but whethe...

journal_title：EMBO reports

authors： Yagi Y,Ip YT

更新日期：2005-11-01 00:00:00