Abstract:
:Central to chemotaxis is the molecular mechanism by which cells exhibit directed movement in shallow gradients of a chemoattractant. We used Dictyostelium mutants to investigate the minimal requirements for chemotaxis, and identified a basal signalling module providing activation of Ras at the leading edge, which is sufficient for chemotaxis. The signalling enzymes PI3K, TorC2, PLA2 and sGC are not required for Ras activation and chemotaxis to folate or to steep gradients of cAMP, but they provide a memory of direction and improved orientation of the cell, which together increase the sensitivity about 150-fold for chemotaxis in shallow cAMP gradients.
journal_name
EMBO Repjournal_title
EMBO reportsauthors
Kortholt A,Kataria R,Keizer-Gunnink I,Van Egmond WN,Khanna A,Van Haastert PJdoi
10.1038/embor.2011.210subject
Has Abstractpub_date
2011-12-01 00:00:00pages
1273-9issue
12eissn
1469-221Xissn
1469-3178pii
embor2011210journal_volume
12pub_type
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