Exploiting antimicrobial resistance: Better knowledge of resistance mechanisms can inform the search for and development of new antibiotics.

abstract：:Chloroplast genes of higher plants are transcribed by two types of RNA polymerase that are encoded by nuclear (NEP (nuclear-encoded plastid RNA polymerase)) or plastid (PEP (plastid-encoded plastid RNA polymerase)) genomes. NEP is largely responsible for the transcription of housekeeping genes during early chloroplast...

journal_title：EMBO reports

authors： Hanaoka M,Kanamaru K,Fujiwara M,Takahashi H,Tanaka K

更新日期：2005-06-01 00:00:00

abstract：:While the genome sequence and gene content are available for an increasing number of organisms, eukaryotic selenoproteins remain poorly characterized. The dual role of the UGA codon confounds the identification of novel selenoprotein genes. Here, we describe a comparative genomics approach that relies on the genome-wi...

journal_title：EMBO reports

authors： Castellano S,Novoselov SV,Kryukov GV,Lescure A,Blanco E,Krol A,Gladyshev VN,Guigó R

更新日期：2004-01-01 00:00:00

abstract：:During the past years, yeasts have been successfully established as models to study the mechanisms of apoptotic regulation. We recently showed that mutations in the LSM4 gene, which is involved in messenger RNA decapping, lead to increased mRNA stability and apoptosis in yeast. Here, we show that mitochondrial functio...

journal_title：EMBO reports

authors： Mazzoni C,Herker E,Palermo V,Jungwirth H,Eisenberg T,Madeo F,Falcone C

更新日期：2005-11-01 00:00:00

abstract：:GlpF, the glycerol facilitator protein of Escherichia coli, is an archetypal member of the aquaporin superfamily. To assess its structure, recombinant histidine-tagged protein was overexpressed, solubilized in octylglucoside and purified to homogeneity. Negative stain electron microscopy of solubilized GlpF protein re...

journal_title：EMBO reports

authors： Braun T,Philippsen A,Wirtz S,Borgnia MJ,Agre P,Kühlbrandt W,Engel A,Stahlberg H

更新日期：2000-08-01 00:00:00

abstract：:The biosynthesis of RNA includes its post-transcriptional modifications, and the crucial functions of these modifications have supported their conservation within all three kingdoms. For example, the modifications located within or adjacent to the anticodon of the transfer RNA (tRNA) enhance the accuracy of codon bind...

journal_title：EMBO reports

authors： Agris PF

更新日期：2008-07-01 00:00:00

abstract：:Epidermal growth factor (EGF) activates the EGF receptor (EGFR) and stimulates its internalization and trafficking to lysosomes for degradation. However, a percentage of EGFR undergoes ligand-independent endocytosis and is rapidly recycled back to the plasma membrane. Importantly, alterations in EGFR recycling are a c...

journal_title：EMBO reports

authors： Ioannou MS,Kulasekaran G,Fotouhi M,Morein JJ,Han C,Tse S,Nossova N,Han T,Mannard E,McPherson PS

更新日期：2017-12-01 00:00:00

abstract：:The developmental and oncogenic roles of MYC proteins are well established, but the transcriptional targets mediating their functions remain elusive. Using small interfering RNA-mediated knockdown in breast and cervix carcinoma cell lines, which overexpress c-MYC, we show that c-MYC independently controls metabolism a...

journal_title：EMBO reports

authors： Cappellen D,Schlange T,Bauer M,Maurer F,Hynes NE

更新日期：2007-01-01 00:00:00

abstract：:Apoptotic cell volume decrease (AVD) and exposure of phosphatidylserine (PtdSer) at the cell surface are early events in apoptosis. However, the ion channels responsible for AVD, and their relationship to PtdSer translocation and cell death are poorly understood. Real-time analysis of calcium-induced apoptosis in lymp...

journal_title：EMBO reports

authors： Elliott JI,Higgins CF

更新日期：2003-02-01 00:00:00

abstract：:Very little is known about the biogenesis and assembly of oligomeric membrane proteins. In this study, the biogenesis of KcsA, a prokaryotic homotetrameric potassium channel, is investigated. Using in vivo pulse-chase experiments, both the monomeric and tetrameric form could be identified. The conversion of monomers i...

journal_title：EMBO reports

authors： van Dalen A,Schrempf H,Killian JA,de Kruijff B

更新日期：2000-10-01 00:00:00

abstract：:The high-mobility-group B (HMGB) chromosomal proteins are characterized by the HMG box, a DNA-binding domain that both introduces a tight bend into DNA and binds preferentially to a variety of distorted DNA structures. The HMGB proteins seem to act primarily as architectural facilitators in the manipulation of nucleop...

journal_title：EMBO reports

authors： Travers AA

更新日期：2003-02-01 00:00:00

abstract：:The EMBO conference 'From Structure to Function of Translocation Machines' took place in April 2013 in Dubrovnik, Croatia. The meeting brought together a mix of established and aspiring researchers to discuss a wealth of unpublished data and ideas in a lively scientific programme of talks designed to shatter the estab...

journal_title：EMBO reports

authors： Lithgow T,Waksman G

更新日期：2013-07-01 00:00:00

abstract：:In response to a variety of stresses, mammalian cells undergo a persistent proliferative arrest known as cellular senescence. Many senescence-inducing stressors are potentially oncogenic, strengthening the notion that senescence evolved alongside apoptosis to suppress tumorigenesis. In contrast to apoptosis, senescent...

journal_title：EMBO reports

authors： Childs BG,Baker DJ,Kirkland JL,Campisi J,van Deursen JM

更新日期：2014-11-01 00:00:00

abstract：:Mammalian artificial chromosomes (MACs) provide a new tool for the improvement of our knowledge of chromosome structure and function. Moreover, they constitute an alternative and potentially powerful tool for gene delivery both in cultured cells and for the production of transgenic animals. In the present work we desc...

journal_title：EMBO reports

authors： Auriche C,Donini P,Ascenzioni F

更新日期：2001-02-01 00:00:00

abstract：:Microbes exert a major impact on human health and disease by either promoting or disrupting homeostasis, in the latter instance leading to the development of infectious diseases. Such disparate outcomes are driven by the ever-evolving genetic diversity of microbes and the countervailing host responses that minimize th...

journal_title：EMBO reports

authors： Soares MP,Weiss G

更新日期：2015-11-01 00:00:00

abstract：:Elongation of the telomeric overhang by telomerase is counteracted by synthesis of the complementary strand by the CST complex, CTC1(Cdc13)/Stn1/Ten1. Interaction of budding yeast Stn1 with overhang-binding Cdc13 is increased by Cdc13 SUMOylation. Human and fission yeast CST instead interact with overhang-binding TPP1...

journal_title：EMBO reports

authors： Garg M,Gurung RL,Mansoubi S,Ahmed JO,Davé A,Watts FZ,Bianchi A

更新日期：2014-08-01 00:00:00

abstract：:The female sex steroid hormone oestrogen stimulates both cell proliferation and cell differentiation in target tissues. These biological actions are mediated primarily through nuclear oestrogen receptors (ERs). The ligand-dependent transactivation of ERs requires several nuclear co-regulator complexes; however, the ce...

journal_title：EMBO reports

authors： Okada M,Takezawa S,Mezaki Y,Yamaoka I,Takada I,Kitagawa H,Kato S

更新日期：2008-06-01 00:00:00

abstract：:The bacterium Bacillus subtilis produces the DNA integrity scanning protein (DisA), a checkpoint protein that delays sporulation in response to DNA damage. DisA scans the chromosome and pauses at sites of DNA lesions. Structural analysis showed that DisA synthesizes the small molecule cyclic diadenosine monophosphate ...

journal_title：EMBO reports

authors： Oppenheimer-Shaanan Y,Wexselblatt E,Katzhendler J,Yavin E,Ben-Yehuda S

更新日期：2011-06-01 00:00:00

abstract：:The Wnt signaling pathway can be grouped into two classes, the β-catenin-dependent and β-catenin-independent pathways. Wnt5a signaling through a β-catenin-independent pathway promotes microtubule (MT) remodeling during cell-substrate adhesion, cell migration, and planar cell polarity formation. Although Wnt5a signalin...

journal_title：EMBO reports

authors： Kikuchi K,Nakamura A,Arata M,Shi D,Nakagawa M,Tanaka T,Uemura T,Fujimori T,Kikuchi A,Uezu A,Sakamoto Y,Nakanishi H

更新日期：2018-07-01 00:00:00

abstract：:Uropathogenic Escherichia coli (UPEC) is the primary cause of symptomatic urinary tract infection. The P-pili, a bacterial surface organelle, mediates the bacterial host--cell adhesion. The PapG adhesin has generated much interest in recent years, not only because of its clinical value, i.e. in the prevention of micro...

journal_title：EMBO reports

authors： Sung MA,Fleming K,Chen HA,Matthews S

更新日期：2001-07-01 00:00:00

abstract：:Around 100 researchers gathered at the eighteenth EMBO Conference on 'The Molecular and Developmental Biology of Drosophila', which took place in Crete in June 2012. Whether deconstructing or integrating at the genetic, cellular or organ level, the talks highlighted the synergy of combining methods, approaches and dis...

journal_title：EMBO reports

authors： Miguel-Aliaga I,Oliver B,Teleman AA

更新日期：2012-11-06 00:00:00

abstract：:The structure of the DNA-binding NAC domain of Arabidopsis ANAC (abscisic-acid-responsive NAC) has been determined by X-ray crystallography to 1.9A resolution (Protein Data Bank codes 1UT4 and 1UT7). This is the first structure determined for a member of the NAC family of plant-specific transcriptional regulators. NAC...

journal_title：EMBO reports

authors： Ernst HA,Olsen AN,Larsen S,Lo Leggio L

更新日期：2004-03-01 00:00:00

abstract：:Nuclear-pore complexes (NPCs) are large protein channels that span the nuclear envelope (NE), which is a double membrane that encloses the nuclear genome of eukaryotes. Each of the typically 2,000-4,000 pores in the NE of vertebrate cells is composed of multiple copies of 30 different proteins known as nucleoporins. T...

journal_title：EMBO reports

authors： Capelson M,Hetzer MW

更新日期：2009-07-01 00:00:00

abstract：:Defects in protein folding and the proteasomal pathway have been linked with many neurodegenerative diseases. PLIC-1 (protein linking IAP to the cytoskeleton) is a ubiquitin-like protein that binds to the ubiquitin-interacting motif (UIM) of the proteasomal subunit S5a. Here, we show that PLIC-1 also binds to the UIM ...

journal_title：EMBO reports

authors： Heir R,Ablasou C,Dumontier E,Elliott M,Fagotto-Kaufmann C,Bedford FK

更新日期：2006-12-01 00:00:00

abstract：:We employed electron cryo-tomography to visualize cytosolic ribosomes on the surface of mitochondria. Translation-arrested ribosomes reveal the clustered organization of the TOM complex, corroborating earlier reports of localized translation. Ribosomes are shown to interact specifically with the TOM complex, and nasce...

journal_title：EMBO reports

authors： Gold VA,Chroscicki P,Bragoszewski P,Chacinska A

更新日期：2017-10-01 00:00:00

abstract：:It has been suggested that DNA methylation/demethylation is involved in regulating V(D)J rearrangement. Although methylated DNA is thought to induce an inaccessible chromatin structure, it is unclear whether DNA methylation can directly control V(D)J recombination independently of chromatin structure. In this study, w...

journal_title：EMBO reports

authors： Nakase H,Takahama Y,Akamatsu Y

更新日期：2003-08-01 00:00:00

abstract：:Although glucocorticoid (GC)-induced nongenomic effects have been reported, the underlying mechanisms remain unexplained. We previously described that lymphocyte-specific protein tyrosine kinase (LCK) and FYN oncogene related to SRC, FGR, YES (FYN) mediate GC-induced inhibition of T-cell-receptor (TCR) signalling. Her...

journal_title：EMBO reports

authors： Löwenberg M,Verhaar AP,Bilderbeek J,Marle Jv,Buttgereit F,Peppelenbosch MP,van Deventer SJ,Hommes DW

更新日期：2006-10-01 00:00:00

abstract：:The Polycomb group (PcG) of proteins conveys epigenetic inheritance of repressed transcriptional states. In Drosophila, the Polycomb repressive complex 1 (PRC1) maintains the silent state by inhibiting the transcription machinery and chromatin remodelling at core promoters. Using immunoprecipitation of in vivo formald...

journal_title：EMBO reports

authors： Breiling A,O'Neill LP,D'Eliseo D,Turner BM,Orlando V

更新日期：2004-10-01 00:00:00

abstract：:In Escherichia coli cells, the origin of chromosomal replication is temporarily inactivated after initiation has occurred. Origin sequestration is the first line of defence against over-initiation, providing a time window during which the initiation potential can be reduced by: (i) titration of DnaA proteins to newly ...

journal_title：EMBO reports

authors： Boye E,Løbner-Olesen A,Skarstad K

更新日期：2000-12-01 00:00:00

abstract：:Various aerolysin-like pore-forming proteins have been identified from bacteria to vertebrates. However, the mechanism of receptor recognition and/or pore formation of the eukaryotic members remains unknown. Here, we present the first crystal and electron microscopy structures of a vertebrate aerolysin-like protein fr...

journal_title：EMBO reports

authors： Jia N,Liu N,Cheng W,Jiang YL,Sun H,Chen LL,Peng J,Zhang Y,Ding YH,Zhang ZH,Wang X,Cai G,Wang J,Dong MQ,Zhang Z,Wu H,Wang HW,Chen Y,Zhou CZ

更新日期：2016-02-01 00:00:00

abstract：:Dynactin has been proposed to link the microtubule-associated motor cytoplasmic dynein with membranous cargo; however, the mechanism by which dynactin-membrane interaction is regulated is unknown. Here we show that dynein and dynactin exist in discrete cytosolic and membrane-bound states in the filamentous fungus Neur...

journal_title：EMBO reports

authors： Kumar S,Zhou Y,Plamann M

更新日期：2001-10-01 00:00:00