Abstract:
:Autophagosome formation is promoted by the PI3 kinase complex and negatively regulated by myotubularin phosphatases, indicating that regulation of local phosphatidylinositol 3-phosphate (PtdIns3P) levels is important for this early phase of autophagy. Here, we show that the Caenorhabditis elegans myotubularin phosphatase MTM-3 catalyzes PtdIns3P turnover late in autophagy. MTM-3 acts downstream of the ATG-2/EPG-6 complex and upstream of EPG-5 to promote autophagosome maturation into autolysosomes. MTM-3 is recruited to autophagosomes by PtdIns3P, and loss of MTM-3 causes increased autophagic association of ATG-18 in a PtdIns3P-dependent manner. Our data reveal critical roles of PtdIns3P turnover in autophagosome maturation and/or autolysosome formation.
journal_name
EMBO Repjournal_title
EMBO reportsauthors
Wu Y,Cheng S,Zhao H,Zou W,Yoshina S,Mitani S,Zhang H,Wang Xdoi
10.15252/embr.201438618subject
Has Abstractpub_date
2014-09-01 00:00:00pages
973-81issue
9eissn
1469-221Xissn
1469-3178pii
embr.201438618journal_volume
15pub_type
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