Abstract:
:Large amounts of DNA are frequently required for use in detection assays and genomic analysis. The limited availability of DNA can be a critical obstacle to meeting research and clinical needs. DNA amplification methods are often required to generate sufficient material from small specimens or environmental samples with low DNA content. The MDA (multiple displacement amplification) reaction is increasingly the method of choice for many applications because of its extensive coverage of the genome, the generation of extremely long DNA products compared with older whole genome amplification methods and the high DNA yields, even from exceedingly low amounts of starting material. Remarkably, MDA enables genomic sequencing even from single microbial cells. Some of the uses of MDA and its strengths and limitations will be discussed.
journal_name
Biochem Soc Transjournal_title
Biochemical Society transactionsauthors
Lasken RSdoi
10.1042/BST0370450subject
Has Abstractpub_date
2009-04-01 00:00:00pages
450-3issue
Pt 2eissn
0300-5127issn
1470-8752pii
BST0370450journal_volume
37pub_type
杂志文章,评审abstract::In this study the network of ATP-consuming and -producing reactions, interacting via the cytosolic ATP/ADP+P(i) system, was studied for the first time in an intact organ, the isolated perfused rat liver, using top-down metabolic-control analysis. Flux control in the metabolically resting state (only oxidative phosphor...
journal_title:Biochemical Society transactions
pub_type: 杂志文章
doi:10.1042/bst0280109
更新日期:2000-02-01 00:00:00
abstract::Dissimilatory metal-reducing bacteria are able to conduct electrons from their cytoplasmic membrane across the periplasm and the outer membrane to redox proteins located on the surface of their cells. The Mtr respiratory pathway in Shewanella is the best-understood metal-reducing pathway to date. The core components o...
journal_title:Biochemical Society transactions
pub_type: 杂志文章
doi:10.1042/BST20120129
更新日期:2012-12-01 00:00:00
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journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/BST0351109
更新日期:2007-11-01 00:00:00
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journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/bst0310134
更新日期:2003-02-01 00:00:00
abstract::Resveratrol (3,4',5-trihydroxystilbene) is found in various plants, including grapes, berries and peanuts. It is also present in wines, especially red wines. During the last years, it has been the focus of numerous in vitro and in vivo studies investigating its biological attributes, which include mainly antioxidant a...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/BST0351156
更新日期:2007-11-01 00:00:00
abstract::The binding sites on human IgG1 for human Fc gamma receptor (Fc gamma R) I, Fc gamma RIIa, Fc gamma RIIb, Fc gamma RIIIa and neonatal FcR have been mapped. A common set of IgG1 residues is involved in binding to all Fc gamma Rs, while Fc gamma RII and Fc gamma RIII utilize distinct sites outside this common set. In ad...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/bst0300487
更新日期:2002-08-01 00:00:00
abstract::Blood vessel formation during vertebrate development relies on a process called angiogenesis and is essential for organ growth and tissue viability. In addition, angiogenesis leads to pathological blood vessel growth in diseases with tissue ischaemia, such as neovascular eye disease and cancer. Neuropilin 1 (NRP1) is ...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/BST20140244
更新日期:2014-12-01 00:00:00
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journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/BST20120009
更新日期:2012-08-01 00:00:00
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journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/BST0330586
更新日期:2005-08-01 00:00:00
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journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/BST0360321
更新日期:2008-06-01 00:00:00
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journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/BST20130142
更新日期:2013-12-01 00:00:00
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journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/BST0371002
更新日期:2009-10-01 00:00:00
abstract::Signalling by pattern recognition receptors (PRRs) is critical for protecting the host against pathogens. Disruption of these signalling pathways has been implicated in many diseases ranging from infection susceptibility to cancer and autoimmune disease. Understanding how PRRs signal is of critical importance due to t...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/BST20160459
更新日期:2017-10-15 00:00:00
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journal_title:Biochemical Society transactions
pub_type: 杂志文章
doi:10.1042/BST20200666
更新日期:2020-12-01 00:00:00
abstract::SUMO (small ubiquitin-related modifier) modification is emerging as an important post-translational control in transcription. In general, SUMO modification is associated with transcriptional repression. Although many SUMO-modified transcription factors and co-activators have been identified, little is known about the ...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/BST0351397
更新日期:2007-12-01 00:00:00
abstract::The calcium-sensing receptor is a multimodal, multimetabolic sensor that mediates the feedback-dependent control of whole body calcium metabolism. Remarkably, in addition to its role in Ca(2+)(o) (extracellular Ca(2+)) sensing, the CaR (Ca(2+)-sensing receptor) also responds to L-amino acids. L-amino acids appear to a...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/BST0351195
更新日期:2007-11-01 00:00:00
abstract::The axial ligand of the catalytic mononuclear T1 copper site (Met(502)) of the CotA laccase was replaced by a leucine or phenylalanine residue to increase the redox potential of the enzyme. These mutations led to an increase in the redox potential by approx. 100 mV relative to the wild-type enzyme but the catalytic co...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/BST0351579
更新日期:2007-12-01 00:00:00
abstract::The Cat family of genes have revealed unexpected complexity and regulation. The first 5 years of research have been productive, yet many important questions remain to be addressed. A major problem for the field of amino acid transport is a severe paucity of data on the structure of these proteins and their arrangement...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/bst0240846
更新日期:1996-08-01 00:00:00
abstract::The examples shown here illustrate the power of gene targeting to the epidermis as a means of developing animal models for the study of human skin diseases. In this short review, I have focused on contributions which stem predominantly from my own laboratory [31, 32, 34, 37, 47]. However, other laboratories have also ...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/bst0191112
更新日期:1991-11-01 00:00:00
abstract::The binding sites of the oestrogen receptors alpha and beta have a structural requirement for ligands with two oxygen atoms (one of which is phenolic) spaced 11-12 A apart. They are open to many non-steroidal compounds, such as those from plants and the chemical industry. The importance of this interaction is the subj...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/0300-5127:0290231
更新日期:2001-05-01 00:00:00
abstract::ESCs (embryonic stem cells) are derived from the inner cell mass of pre-implantation embryos and are pluripotent, meaning they can differentiate into all of the cells that make up the adult organism. This property of pluripotency makes ESCs attractive as a model system for studying early development and for the genera...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/BST0390674
更新日期:2011-04-01 00:00:00
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journal_title:Biochemical Society transactions
pub_type: 杂志文章
doi:10.1042/bst0210039
更新日期:1993-02-01 00:00:00
abstract::The mitotic spindle is the macromolecular machine utilized to accurately segregate chromosomes in cells. How this self-organized structure assembles is a key aspect of understanding spindle morphogenesis. In the present review, we focus on understanding mechanisms of spindle self-assembly and address how subcellular s...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/BST20140243
更新日期:2015-02-01 00:00:00
abstract::For a disease such as cancer, where a number of alterations to normal cell function accumulate over time, there are several opportunities to inhibit, slow down or even reverse the process. Many of the changes which drive the disease process occur in cell-signalling pathways that regulate proliferation and apoptosis. A...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/bst0280007
更新日期:2000-02-01 00:00:00
abstract::A novel strategy to treat anxiety and fear-related disorders such as phobias, panic and PTSD (post-traumatic stress disorder) is combining CBT (cognitive behavioural therapy), including extinction-based exposure therapy, with cognitive enhancers. By targeting and boosting mechanisms underlying learning, drug developme...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/BST20130233
更新日期:2014-04-01 00:00:00
abstract::Modulation of traits beneficial for cultivation and yield is one of the main goals of crop improvement. One of the targets for enhancing productivity is changing the architecture of inflorescences since in many species it determines fruit and seed yield. Inflorescence shape and organization is genetically established ...
journal_title:Biochemical Society transactions
pub_type: 杂志文章
doi:10.1042/BST20190441
更新日期:2020-06-30 00:00:00
abstract::Escherichia coli FtsK is a septum-located DNA translocase that co-ordinates the late stages of cytokinesis and chromosome segregation. Relatives of FtsK are present in most bacteria; in Bacillus subtilis, the FtsK orthologue, SpoIIIE, transfers the majority of a chromosome into the forespore during sporulation. DNA tr...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/BST0380395
更新日期:2010-04-01 00:00:00
abstract::Although it is now well established that PI3K (phosphoinositide 3-kinase) is a key enzyme in several intracellular processes, there are still relatively few reports that precisely identify the specific isoforms of PI3K actually involved in such events. The lack of specific inhibitors has made it particularly difficult...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/BST0350211
更新日期:2007-04-01 00:00:00
abstract::Proline-rich cell-penetrating peptides, particularly the SAP (sweet arrow peptide), (VRLPPP)(3), have been proposed to be useful intracellular delivery vectors, as a result of their lack of cytotoxicity combined with their capacity to be internalized by cells. A common limitation of the therapeutic use of peptides is ...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/BST0350794
更新日期:2007-08-01 00:00:00
abstract::Pantothenate (vitamin B5) is a water-soluble vitamin essential for the synthesis of CoA and ACP (acyl-carrier protein, cofactors in energy yielding reactions including carbohydrate metabolism and fatty acid synthesis. Pantothenate is synthesized de novo by plants and micro-organisms; however, animals obtain the vitami...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/BST0330743
更新日期:2005-08-01 00:00:00