Abstract:
:Although neural stem cell (NSC) transplantation has been investigated as a promising tool for reconstituting damaged brains, recent evidences suggest that NSCs may rescue the brain via paracrine effects rather than by direct cell replacements. In this study, we attempted to determine the neuroprotective effect of NSC-conditioned media (NSC-CM) in in vitro model of Huntington's disease. Cerebral hybrid neurons (A1) were transfected with either wild-type huntingtin (18 CAG repeats) or mutant huntingtin (100 CAG repeats). At 24h after the transfection, immunocytochemical patterns of the huntingtin aggregations, as well as the level of N-terminal proteolytic cleavages of huntingtin were analyzed. Neuronal apoptosis was evaluated with flowcytometry after Annexin-V and propidium iodide (PI) staining. Cerebral hybrid neurons transfected with mutant huntingtin showed five aggregates patterns, including diffuse cytoplasmic, dispered vacuoles, perinuclear vacuoles, nuclear inclusions (NI), and cytoplasmic inclusions (CI). NSC-CM reduced the levels of nuclear and cytoplasmic inclusions. The transfection with mutant huntingtin increased the level of N-terminal cleavages, which was reduced by the NSC-CM treatment. In addition, NSC-CM reduced the Annexin-V(+)PI(+) and Annexin-V(+)PI(-) neurons which were induced by the mutant huntingtin transfection. In summary, NSC-CM was neuroprotective in in vitro model of Huntington's disease with modulating mutant huntingtin-induced cytotoxicity.
journal_name
Neurosci Lettjournal_title
Neuroscience lettersauthors
Lim HC,Lee ST,Chu K,Joo KM,Kang L,Im WS,Park JE,Kim SU,Kim M,Cha CIdoi
10.1016/j.neulet.2008.02.035subject
Has Abstractpub_date
2008-04-25 00:00:00pages
175-80issue
3eissn
0304-3940issn
1872-7972pii
S0304-3940(08)00220-6journal_volume
435pub_type
杂志文章abstract::In recent years, animal behavioral models, particularly those used in pain research, have been increasingly scrutinized and criticized for their role in the poor translation of novel pharmacotherapies for chronic pain. This article addresses the use of animal models of pain from the perspective of industrial drug disc...
journal_title:Neuroscience letters
pub_type: 杂志文章,评审
doi:10.1016/j.neulet.2013.08.033
更新日期:2013-12-17 00:00:00
abstract::Transcranial direct current stimulation (tDCS) is a non-invasive technique that moderates cognitive and motor function. The effects of tDCS on cognitive and motor tasks vary among individuals. However, the source of the inter-individual variability remains unknown. The purpose of the present study was to examine wheth...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2013.04.022
更新日期:2013-06-17 00:00:00
abstract::Na+, K+-ATPase is one of major membrane proteins that has two subunits, alpha and beta. The alpha subunit has the ATPase activity and the ouabain binding site. Among four isoforms of the alpha subunit, expression of alpha1, alpha2, and alpha3, but not alpha4, is observed in matured rat brain. Ouabain is one of cardiac...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2006.11.061
更新日期:2007-02-08 00:00:00
abstract::Presentation of two different tones at two different time periods each under two different types (14C or 3H) of 2-deoxyglucose (2-DG) produced two bands of labelling in the inferior colliculus (IC) of a guinea pig. Filtering tests revealed that both bands were produced by only one of the labels (14C). Four guinea pigs...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(83)90052-6
更新日期:1983-10-10 00:00:00
abstract::Hemispheric asymmetries in spatial processing are generally considered to be responsible for the shorter reaction time (RT) of the left hand classically observed for right-handers when pointing at targets. Surprisingly, despite the special role which the right cerebral hemisphere is known to play in visual attention, ...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(01)02313-8
更新日期:2001-11-23 00:00:00
abstract::In this study the effect of memantine, an antagonist at the N-methyl-D-aspartate receptor, on spatial learning deficit and on neuronal damage following transient cerebral ischemia was evaluated. Global ischemia was induced by four-vessel-occlusion (4VO) for 20 min in rats. Memantine was administered 20 min before indu...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(96)12545-3
更新日期:1996-04-12 00:00:00
abstract::The aim of the present study was to examine the patterns of activation of the P600 waveform of the event-related potentials (ERP), applying principal component analysis (PCA) and repeated measures ANOVA, and whether these patterns are RF and gender dependent. The ERPs of thirty-nine healthy subjects (20 male and 19 fe...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2010.04.058
更新日期:2010-06-30 00:00:00
abstract::Substance P (SP) plays an important role in sensitization of spinal cord neurons. In this study, we investigated SP-induced calcium activities in cultured rat spinal cord neurons with confocal laser scanning microscopy. Our results showed that SP increased [Ca(2+)](i) by calcium entry rather than release from intracel...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(00)01135-6
更新日期:2000-06-16 00:00:00
abstract::8-Hydroxy-2-[di-n-propylamino]-tetralin) (8-OH-DPAT), a 5-HT1A/7 receptor agonist, has a chronobiological effect on the circadian system. To identify how the 8-OH-DPAT exerts this effect, we specifically destroyed the serotonergic (5-HT) fibres connecting the median raphe nuclei (RN) to the suprachiasmatic nuclei (SCN...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(98)00749-6
更新日期:1998-10-30 00:00:00
abstract::A pronounced decrease in dopamine content in both the striatum and the mesolimbic area was observed up to 120 days of age after treatment with 6-hydroxydopamine (100 micrograms, intracisternally) following desipramine pretreatment (20 mg/kg, i.p.) at the 5th postnatal day. In the 6-hydroxydopamine-treated rats, Met-en...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(88)90154-1
更新日期:1988-04-22 00:00:00
abstract::Cognitive impairment in Parkinson's disease is accompanied by a marked decrease of cerebrocortical nicotinic receptors. To study the putative site of impaired receptor synthesis, frontal cortices of Parkinson patients with cognitive dysfunction have been screened for the expression of the nicotinic receptor alpha 4 su...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(95)11367-6
更新日期:1995-03-10 00:00:00
abstract::Presence of substance P-like immunoreactive neurons and nerve fibers is demonstrated in the trigeminal ganglion of newborn and adult human subjects by the indirect immunofluorescence technique. Two populations of neurons containing high and low densities of immunoreactive material, respectively, are identified. Morpho...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(90)90780-d
更新日期:1990-03-02 00:00:00
abstract::It is known that alcohol induces disorders in the metabolism of retinoids and particularly in the biosynthetic pathways of retinoic acid (RA). Since RA has, along with other hormones and particularly triiodothyronine (T3), a physiological role in the adult brain, the effect of chronic exposure to alcohol on RA and T3 ...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(96)12455-1
更新日期:1996-03-15 00:00:00
abstract:BACKGROUND:Studies have demonstrated that the failure of oligodendrocyte precursor cells (OPCs) differentiation as a major cause of remyelination failure in demyelinating disease. The reasons for this failure are not completely understood. We hypothesized that the present of myelin debris in CNS play an important role ...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2020.134872
更新日期:2020-04-23 00:00:00
abstract::Cerebellar Purkinje cells differentiate the most elaborate dendritic trees among neurons in the brain and constitute the principal part of cerebellar neuronal circuitry. In the present study, we examined the role of the GluA2 subunit of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-type glutamate recepto...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2017.07.054
更新日期:2017-09-14 00:00:00
abstract::In vivo L-band electron spin resonance (ESR) system was used to measure the decay of nitroxide radicals in the living rat cerebrum containing a cortical area with an iron-induced epileptogenic region. Carbamoyl-PROXYL (3-carbamoyl-2,2,5,5-tetramethyl-1-pyrrolinyloxy) was injected intraperitoneally. The decay of nitrox...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(96)12412-5
更新日期:1996-03-01 00:00:00
abstract::The somatosensory projection to the striatum of the rat was investigated by electrophysiological mapping. Fifty-five units were encountered that responded to specific forms of stimulation delivered to particular parts of the body. Units that responded to stimuli delivered to the hindlimb, scrotum and tail were found i...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(82)90405-0
更新日期:1982-06-30 00:00:00
abstract::Spinocerebellar ataxias (SCAs) are a group of clinically and genetically heterogeneous neurological diseases. The expansion of unstable microsatellite repeats has been identified as the underlying pathogenic cause of 10 subtypes of autosomal dominant SCAs. The aetiology of sporadic SCA is unknown. The aim of this stud...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2007.09.055
更新日期:2007-12-11 00:00:00
abstract::A tight coupling between lexico-semantic access and motor control has been established on the basis of neuropsychological, neurophysiological, and behavioral evidence. For example, sensory and motor cortices have been shown to be active when subjects listen to words denoting bodily actions. Kinematic analyses of subje...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2013.09.021
更新日期:2013-10-25 00:00:00
abstract::Using retrograde transport of Fluoro-Gold (FG) combined with immunofluorescence for phenylethanolamine-N-methyltransferase (PNMT), we have examined afferents to the nucleus locus coeruleus (LC) from the rostral ventrolateral medulla (nucleus paragigantocellularis; PGi) in rats sustaining lesions of the medullary adren...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(90)90612-d
更新日期:1990-10-16 00:00:00
abstract::An intraperitoneal injection of 0.1 nmol uridine in rats resulted in a transient excess slow-wave sleep if administered shortly before onset of the dark period. The sleep latency was remarkably shortened. A small dose (0.01 nmol) and larger doses (1, 10, 100 nmol) caused no effect. Uridine at a dose of 0.1 nmol was en...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(85)90297-6
更新日期:1985-11-20 00:00:00
abstract::The effect of acute phenylethanolamine-N-methyltransferase (PNMT) inhibition on rat brain catecholamine concentrations was studied. All PNMT inhibitors tested were effective in depleting hypothalamic and brain stem epinephrine. Norepinephrine concentrations were unaffected; however, dopamine levels were increased in a...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(80)90235-9
更新日期:1980-10-20 00:00:00
abstract::The present study tests the hypothesis that nitration is a potential mechanism of N-methyl-D-aspartate (NMDA) receptor modification, by assessing the effect of peroxynitrite in vitro on the glutamate and ion-channel sites of the NMDA receptor in the fetal guinea pig. Nitration of NMDA receptor subunits was confirmed b...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(00)01608-6
更新日期:2000-12-15 00:00:00
abstract::Precise evaluation of the ascending reticular activating system (ARAS) is important for diagnosis, prediction of prognosis, and management of patients with disorders of impaired consciousness. In the current study, we attempted to reconstruct the direct neural pathway between the brainstem reticular formation (RF) and...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2015.09.004
更新日期:2015-10-08 00:00:00
abstract::Since calcium homeostasis is altered in cultured skin fibroblasts from aged and Alzheimer donors, the present study examined the degradation of spectrin, a substrate of the calcium dependent protease calpain. Spectrin proteolysis was estimated as the percentage of spectrin breakdown products (e.g., 150 + 155 kDa bands...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(91)90694-o
更新日期:1991-01-02 00:00:00
abstract::P2X receptors (P2XRs) are a family of ATP-gated ionic channels that are expressed in numerous excitable and non-excitable cells. Despite the great advance on the structure and function of these receptors in the last decades, there is still lack of specific and potent antagonists for P2XRs subtypes, especially for the ...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2018.10.047
更新日期:2019-01-18 00:00:00
abstract::In vivo rates of incorporation and turnover of palmitate and arachidonate in brain phospholipids were measured in awake rats treated chronically with lithium, following intravenous infusion of radiolabeled palmitate and arachidonate, respectively. Chronic lithium, at a brain level considered to be therapeutic in human...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(96)13264-x
更新日期:1996-12-20 00:00:00
abstract::The present study examines the online realization of pragmatic meaning using event-related potentials (ERPs). Participants read sentences including the English quantifier some, which has both a semantic meaning (at least one) and a pragmatic meaning (not all). Unlike previous ERP studies of this phenomenon, sentences ...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2012.11.044
更新日期:2013-02-08 00:00:00
abstract::Glucagon-like peptide-1 (GLP-1), an insulinotropic peptide secreted from enteroendocrine cells, has been known to have a neuroprotective effect. However, it is not fully understood the intracellular mediator of GLP-1 signaling in neuronal cells. In the present study, we examined the change in intracellular signaling o...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2016.01.052
更新日期:2016-03-11 00:00:00
abstract::The pattern of projection of the ventrobasal thalamic complex (VB) upon the neocortical barrel field of normal and reeler mutant mice was determined by orthograde degeneration experiments and by Timm's histochemical method. In both reeler and normal mice the thalamic terminals are concentrated at the midcortical zone ...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(76)90091-4
更新日期:1976-09-01 00:00:00