Site-specific cleavage by metal ion cofactors and inhibitors of M1 RNA, the catalytic subunit of RNase P from Escherichia coli.

abstract：:Translational initiation factor 2 (IF2) is a guanine nucleotide-binding protein that can bind guanosine 3',5'-(bis) diphosphate (ppGpp), an alarmone involved in stringent response in bacteria. In cells growing under optimal conditions, the GTP concentration is very high, and that of ppGpp very low. However, under stre...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Milon P,Tischenko E,Tomsic J,Caserta E,Folkers G,La Teana A,Rodnina MV,Pon CL,Boelens R,Gualerzi CO

更新日期：2006-09-19 00:00:00

abstract：:Here, we describe the identification and characterization of a nuclear body (matrix-associated deacetylase body) whose formation and integrity depend on deacetylase activity. Typically, there are 20-40 0.5-microM bodies per nucleus, although the size and number can vary substantially. The structure appears to contain ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Downes M,Ordentlich P,Kao HY,Alvarez JG,Evans RM

更新日期：2000-09-12 00:00:00

abstract：:Most B lymphocytes in normal individuals express two classes of cell-surface immunoglobulins, IgM and IgD. The specificity of the two antigen receptors is identical since they are produced by transcription and differential splicing of the same variable region gene segment to the heavy-chain constant region gene segmen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Irsch J,Irlenbusch S,Radl J,Burrows PD,Cooper MD,Radbruch AH

更新日期：1994-02-15 00:00:00

abstract：:We provide here evidence that supports the occurrence of a biologically dormant form of selectin ligand carbohydrate, the sialyl 6-sulfo Lewis X containing modified sialic acid, in human leukocytes. The modification of sialic acid involves first de-N-acetylation of sialic acid moiety through ubiquitous de-N-acetylatio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mitsuoka C,Ohmori K,Kimura N,Kanamori A,Komba S,Ishida H,Kiso M,Kannagi R

更新日期：1999-02-16 00:00:00

abstract：:The pattern of neurodegeneration in Alzheimer's disease (AD) is very distinctive: neurofibrillary tangles (NFTs) composed of hyperphosphorylated tau selectively affect pyramidal neurons of the aging association cortex that interconnect extensively through glutamate synapses on dendritic spines. In contrast, primary se...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Carlyle BC,Nairn AC,Wang M,Yang Y,Jin LE,Simen AA,Ramos BP,Bordner KA,Craft GE,Davies P,Pletikos M,Šestan N,Arnsten AF,Paspalas CD

更新日期：2014-04-01 00:00:00

abstract：:In most macroscale robotic systems, propulsion and controls are enabled through a physical tether or complex onboard electronics and batteries. A tether simplifies the design process but limits the range of motion of the robot, while onboard controls and power supplies are heavy and complicate the design process. Here...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen T,Bilal OR,Shea K,Daraio C

更新日期：2018-05-29 00:00:00

abstract：:c-Mpl, a member of the hematopoietic cytokine receptor family, is the receptor for thrombopoietin. To investigate signal transduction by c-Mpl, a chimeric receptor, composed of the extracellular domain of human growth hormone receptor and the intracellular domain of c-Mpl, was introduced into the interleukin 3-depende...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gurney AL,Wong SC,Henzel WJ,de Sauvage FJ

更新日期：1995-06-06 00:00:00

abstract：:The beta 2-adrenergic receptor (beta 2AR) can be constitutively activated by mutations in the third intracellular loop. Whereas the wild-type receptor exists predominantly in an inactive conformation (R) in the absence of agonist, the mutant receptor appears to spontaneously adopt an active conformation (R*). We now d...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pei G,Samama P,Lohse M,Wang M,Codina J,Lefkowitz RJ

更新日期：1994-03-29 00:00:00

abstract：:Cancer therapies using genes and other macromolecules might realize their full clinical potential if they could be delivered to tumor tissue in optimal quantities. Unfortunately, the compromised circulation within tumors poses a formidable resistance to adequate and uniform penetration of these agents. Previously, we ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Netti PA,Hamberg LM,Babich JW,Kierstead D,Graham W,Hunter GJ,Wolf GL,Fischman A,Boucher Y,Jain RK

更新日期：1999-03-16 00:00:00

abstract：:In mantle cell lymphoma (MCL), the translocation t(11;14) is considered the cytogenetic hallmark of the disease. Recently, however, deletion of the chromosomal region 11q22-q23 has been identified as a frequent event in this type of cancer, indicating the existence of a pathogenically relevant tumor suppressor gene in...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schaffner C,Idler I,Stilgenbauer S,Döhner H,Lichter P

更新日期：2000-03-14 00:00:00

abstract：:During development, organisms acquire three-dimensional (3D) shapes with important physiological consequences. While basic mechanisms underlying morphogenesis are known in eukaryotes, it is often difficult to manipulate them in vivo. To circumvent this issue, here we present a study of developing Vibrio cholerae biofi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fei C,Mao S,Yan J,Alert R,Stone HA,Bassler BL,Wingreen NS,Košmrlj A

更新日期：2020-04-07 00:00:00

abstract：:Complexin activates Ca(2+)-triggered neurotransmitter release and regulates spontaneous release in the presynaptic terminal by cooperating with the neuronal soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) and the Ca(2+)-sensor synaptotagmin. The N-terminal domain of complexin is importa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lai Y,Choi UB,Zhang Y,Zhao M,Pfuetzner RA,Wang AL,Diao J,Brunger AT

更新日期：2016-08-09 00:00:00

abstract：:In a medium without Na+, gamma-aminobutyric acid (GABA) binds at 0 degrees to freshly prepared crude synaptic membranes from rat cerebral cortex with an apparent dissociation constant of 218 nM. An endogenous inhibitor of the Na+-independent GABA binding was removed from these membranes by freezing and thawing and by ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Toffano G,Guidotti A,Costa E

更新日期：1978-08-01 00:00:00

abstract：:An RNA-directed DNA polymerase was isolated from the peripheral blood leukocytes of a patient with acute myelomonocytic leukemia by successive purification of a particulate cytoplasmic fraction with endogenous, ribonuclease-sensitive DNA polymerase activity. Like RNA-directed DNA polymerase from mammalian type-C virus...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gallagher RE,Todaro GJ,Smith RG,Livingston DM,Gallo RC

更新日期：1974-04-01 00:00:00

abstract：:The importance of founder events in promoting evolutionary changes on islands has been a subject of long-running controversy. Resolution of this debate has been hindered by a lack of empirical evidence from naturally founded island populations. Here we undertake a genetic analysis of a series of historically documente...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Clegg SM,Degnan SM,Kikkawa J,Moritz C,Estoup A,Owens IP

更新日期：2002-06-11 00:00:00

abstract：:Senescence is controlled by time-evolving networks that describe the temporal transition of interactions among senescence regulators. Here, we present time-evolving networks for NAM/ATAF/CUC (NAC) transcription factors in Arabidopsis during leaf aging. The most evident characteristic of these time-dependent networks w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kim HJ,Park JH,Kim J,Kim JJ,Hong S,Kim J,Kim JH,Woo HR,Hyeon C,Lim PO,Nam HG,Hwang D

更新日期：2018-05-22 00:00:00

abstract：:Historical collapse of ancient states or civilizations has raised new awareness about its possible relevance to current issues of sustainability, in the context of global change. This Special Feature examines 12 case studies of societies under stress, of which seven suffered severe transformation. Outcomes were comple...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Butzer KW,Endfield GH

更新日期：2012-03-06 00:00:00

abstract：:A joint analysis of all-atom molecular dynamics (MD) calculations and picosecond time-resolved x-ray structures was performed to gain single-molecule insights into mechanisms of protein function. Ensemble-averaged MD simulations of the L29F mutant of myoglobin after ligand dissociation reproduce the direction, amplitu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hummer G,Schotte F,Anfinrud PA

更新日期：2004-10-26 00:00:00

abstract：:From a flounder pituitary cDNA library, cDNA clones encoding a 28-kDa glycoprotein produced by the pars intermedia of the pituitary were isolated and characterized. Nucleotide sequencing demonstrated a precursor of the 28-kDa protein, which consisted of 231 amino acid residues, to be cleaved into a signal peptide (24 ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ono M,Takayama Y,Rand-Weaver M,Sakata S,Yasunaga T,Noso T,Kawauchi H

更新日期：1990-06-01 00:00:00

abstract：:The mechanisms involved in the uptake of uni- and multi-lamellar lipid vesicles by BALB/c mouse 3T3 cells have been investigated. Vesicles are incorporated into cells both by endocytosis and by a nonendocytotic mechanism which we propose involves fusion of vesicles with the plasma membrane. The nonendocytotic pathway ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Poste G,Papahadjopoulos D

更新日期：1976-05-01 00:00:00

abstract：:We have used a semiselective Hahn spin-echo sequence of the form (1331)-tau-(2662)-tau-AQ, delivered by a surface coil to obtain high-resolution 1H NMR spectra from the brains of intact dead rats. This sequence gave suppression of the tissue water resonance by a factor of 80,000 when tau = 68 ms. Delivery of a frequen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hetherington HP,Avison MJ,Shulman RG

更新日期：1985-05-01 00:00:00

abstract：:We describe the effect of a 200-microns wavelength free electron laser beam on the ability of asynchronized and synchronized mammalian tissue culture cells to incorporate tritiated thymidine. Compared to controls (unexposed cells), a significant proportion of exposed cells exhibited a reduction in isotope incorporatio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Berns MW,Bewley W,Sun CH,Templin P

更新日期：1990-04-01 00:00:00

abstract：:To elucidate cellular diversity and clonal evolution in tissues and tumors, one must resolve genomic heterogeneity in single cells. To this end, we have developed low-cost, mass-producible micro-/nanofluidic chips for DNA extraction from individual cells. These chips have modules that collect genomic DNA for sequencin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Marie R,Pedersen JN,Bærlocher L,Koprowska K,Pødenphant M,Sabatel C,Zalkovskij M,Mironov A,Bilenberg B,Ashley N,Flyvbjerg H,Bodmer WF,Kristensen A,Mir KU

更新日期：2018-10-30 00:00:00

abstract：:Global activation of the embryonic genome is the most critical event in early mammalian development. After fertilization, a rich supply of maternal proteins and RNAs support development whereas a number of zygotic and embryonic genes are expressed in a stage-specific manner leading to embryonic genome activation (EGA)...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Misirlioglu M,Page GP,Sagirkaya H,Kaya A,Parrish JJ,First NL,Memili E

更新日期：2006-12-12 00:00:00

abstract：:We have previously shown that lymphoblasts alloactivated in vitro acquire the capacity of stimulating the autologous mixed lymphocyte response. This response is anti-idiotypic in nature because lymphocytes so primed display accelerated memory responses only when restimulated by autologous lymphoblasts that have been a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Suciu-Foca N,Reed E,Rohowsky C,Kung P,King DW

更新日期：1983-02-01 00:00:00

abstract：:The crystal structure of a recombinant alphaEC domain from human fibrinogen-420 has been determined at a resolution of 2.1 A. The protein, which corresponds to the carboxyl domain of the alphaE chain, was expressed in and purified from Pichia pastoris cells. Felicitously, during crystallization an amino-terminal segme...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Spraggon G,Applegate D,Everse SJ,Zhang JZ,Veerapandian L,Redman C,Doolittle RF,Grieninger G

更新日期：1998-08-04 00:00:00

abstract：:Embryonic stem cells (ESCs) represent an important research tool and a potential resource for regenerative medicine. Generally, ESCs are cocultured with a supportive feeder cell layer of murine embryonic fibroblasts, which maintain the ESCs' capacity for self-renewal and block spontaneous differentiation. These cumber...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miyabayashi T,Teo JL,Yamamoto M,McMillan M,Nguyen C,Kahn M

更新日期：2007-03-27 00:00:00

abstract：:The biosynthesis of specific polypeptides directed by purified viral messenger RNA from JLS-V9 cells infected with Rauscher leukemia virus has been studied in a rabbit reticulocyte lysate. The 35S viral mRNA gives rise to two major products of 65,000 and 72,000 molecular weight. The synthesis of specific polypeptides ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gielkens AL,Van Zaane D,Bloemers HP,Bloemendal H

更新日期：1976-02-01 00:00:00

abstract：:In the human, cone photoreceptors (L, M, and S) and the melanopsin-containing, intrinsically photosensitive retinal ganglion cells (ipRGCs) are active at daytime light intensities. Signals from cones are combined both additively and in opposition to create the perception of overall light and color. Similar mechanisms ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Spitschan M,Jain S,Brainard DH,Aguirre GK

更新日期：2014-10-28 00:00:00

abstract：:Mice with a targeted mutation of the gastric inhibitory polypeptide (GIP) receptor gene (GIPR) were generated to determine the role of GIP as a mediator of signals from the gut to pancreatic beta cells. GIPR-/- mice have higher blood glucose levels with impaired initial insulin response after oral glucose load. Althou...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miyawaki K,Yamada Y,Yano H,Niwa H,Ban N,Ihara Y,Kubota A,Fujimoto S,Kajikawa M,Kuroe A,Tsuda K,Hashimoto H,Yamashita T,Jomori T,Tashiro F,Miyazaki J,Seino Y

更新日期：1999-12-21 00:00:00