Abstract:
:Here, we describe the identification and characterization of a nuclear body (matrix-associated deacetylase body) whose formation and integrity depend on deacetylase activity. Typically, there are 20-40 0.5-microM bodies per nucleus, although the size and number can vary substantially. The structure appears to contain both class I and the recently described class II histone deacetylases (HDAC)5 and 7 along with the nuclear receptor corepressors SMRT (silencing mediator for retinoid and thyroid receptor) and N-CoR (nuclear receptor corepressor). Addition of the deacetylase inhibitors trichostatin A and sodium butyrate completely disrupt these nuclear bodies, providing a demonstration that the integrity of a nuclear body is enzyme dependent. We demonstrate that HDAC5 and 7 can associate with at least 12 distinct proteins, including several members of the NuRD and Sin3A repression complexes, and appear to define a new but related complex.
journal_name
Proc Natl Acad Sci U S Aauthors
Downes M,Ordentlich P,Kao HY,Alvarez JG,Evans RMdoi
10.1073/pnas.97.19.10330keywords:
subject
Has Abstractpub_date
2000-09-12 00:00:00pages
10330-5issue
19eissn
0027-8424issn
1091-6490pii
97/19/10330journal_volume
97pub_type
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