Regulation of Cl- transport in T84 cell clones expressing a mutant regulatory subunit of cAMP-dependent protein kinase.

abstract：:During the late summer to fall of 1987, Caribbean reef corals experienced an intense and widespread discoloration event described as bleaching. Contrary to initial predictions, most bleached corals did not die. However, energy input from zooxanthellae decreased, as estimated from: (i) delta(13)C values, a measure of t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Porter JW,Fitt WK,Spero HJ,Rogers CS,White MW

更新日期：1989-12-01 00:00:00

abstract：:Mutations responsible for inherited disease may act by disrupting normal transcriptional splicing. Such mutations can be difficult to detect, and their effects difficult to characterize, because many lie deep within exons or introns where they may alter splice enhancers or silencers or introduce new splice acceptors o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Casadei S,Gulsuner S,Shirts BH,Mandell JB,Kortbawi HM,Norquist BS,Swisher EM,Lee MK,Goldberg Y,O'Connor R,Tan Z,Pritchard CC,King MC,Walsh T

更新日期：2019-12-16 00:00:00

abstract：:The development of seeds in flowering plants is placed under complex interactions between maternal tissues, the embryo, and the endosperm. The endosperm plays a major role in the regulation of seed size. In Arabidopsis thaliana, endosperm size depends on the coordination of the genetic pathway HAIKU (IKU) with epigene...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li J,Nie X,Tan JL,Berger F

更新日期：2013-09-17 00:00:00

abstract：:The peptide chain release factor 2 (RF2) gene, prfB, was cloned from Salmonella typhimurium by DNA hybridization using the Escherichia coli prfB probe. The nucleotide and amino acid sequences of prfB are 87.0% and 95.6% homologous between E. coli and S. typhimurium, respectively, including an in-frame premature UGA st...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kawakami K,Nakamura Y

更新日期：1990-11-01 00:00:00

abstract：:The role of the serum proteolytic system plasminogen/plasminogen activator as a biochemical tool used by the glia or neurons, or both, for maintaining their temporary and flexible cellular interactions during histogenesis of the nervous system is under study. The present report identifies a glia cell type, the Schwann...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kalderon N

更新日期：1984-11-01 00:00:00

abstract：:We have shown previously that guanine nucleotide-binding protein (G protein) beta gamma complexes purified from bovine brain membranes are methyl esterified on a C-terminal cysteine residue of the gamma polypeptide. In the present study, 3H-methylated G beta gamma complexes cleaved to their constituent amino acids by ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yamane HK,Farnsworth CC,Xie HY,Howald W,Fung BK,Clarke S,Gelb MH,Glomset JA

更新日期：1990-08-01 00:00:00

abstract：:The auditory cortex is critical for perceiving a sound's location. However, there is no topographic representation of acoustic space, and individual auditory cortical neurons are often broadly tuned to stimulus location. It thus remains unclear how acoustic space is represented in the mammalian cerebral cortex and how...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miller LM,Recanzone GH

更新日期：2009-04-07 00:00:00

abstract：:Serum amyloid A (SAA) is an acute-phase plasma protein that functions in innate immunity and lipid homeostasis. SAA is a protein precursor of reactive AA amyloidosis, the major complication of chronic inflammation and one of the most common human systemic amyloid diseases worldwide. Most circulating SAA is protected f...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jayaraman S,Gantz DL,Haupt C,Gursky O

更新日期：2017-08-08 00:00:00

abstract：:The expression of at least 24 distinct genes of Pseudomonas aeruginosa PAO1 is under direct control of the "ferric uptake regulator" (Fur). Novel targets of the Fur protein were isolated in a powerful SELEX (systematic evolution of ligands by exponential enrichment)-like cycle selection consisting of in vitro DNA-Fur ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ochsner UA,Vasil ML

更新日期：1996-04-30 00:00:00

abstract：:The amino-terminal sequences of two polypeptides with nonsuppressible insulin-like and cell-growth-promoting activities (NSILA I and II), isolated from human serum, were determined. Of the first 31 residues, 22 are identical in NSILA I and II. Moreover, a striking structural similarity was found between NSILA and insu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rinderknecht E,Humbel RE

更新日期：1976-12-01 00:00:00

abstract：:Single-cell RNA sequencing (scRNA-seq) enables the quantification of each gene's expression distribution across cells, thus allowing the assessment of the dispersion, nonzero fraction, and other aspects of its distribution beyond the mean. These statistical characterizations of the gene expression distribution are cri...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang J,Huang M,Torre E,Dueck H,Shaffer S,Murray J,Raj A,Li M,Zhang NR

更新日期：2018-07-10 00:00:00

abstract：:Column chromatography on (dT)(12-18)-cellulose was used to detect and isolate a novel DNA polymerase activity from human breast tumor cell line HBT-3. The new enzyme is similar to those of mammalian RNA tumor viruses in its molecular size, preference for poly(A).(dT)(12-18) as compared to either poly(dA).(dT)(12-18) o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gerwin BI,Bassin RH

更新日期：1973-08-01 00:00:00

abstract：:Klotho is a circulating protein, and Klotho deficiency disturbs endothelial integrity, but the molecular mechanism is not fully clarified. We report that vascular endothelium in Klotho-deficient mice showed hyperpermeability with increased apoptosis and down-regulation of vascular endothelial (VE)-cadherin because of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kusaba T,Okigaki M,Matui A,Murakami M,Ishikawa K,Kimura T,Sonomura K,Adachi Y,Shibuya M,Shirayama T,Tanda S,Hatta T,Sasaki S,Mori Y,Matsubara H

更新日期：2010-11-09 00:00:00

abstract：:Lipid droplets (LDs) are a conserved feature of most organisms. Vertebrate adipocytes have evolved to efficiently store and release lipids for the whole organism from a single droplet. Perilipin 1, the most abundant lipid-coat protein in adipocytes, plays a key role in regulating lipolysis. In other tissues such as li...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Patel S,Yang W,Kozusko K,Saudek V,Savage DB

更新日期：2014-06-24 00:00:00

abstract：:Because RNAs fold as they are being synthesized, their transcription rate can affect their folding. Here, we report the results of single-molecule fluorescence studies that characterize the ligand-dependent cotranscriptional folding of the Escherichia coli thiM riboswitch that regulates translation. We found that the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Uhm H,Kang W,Ha KS,Kang C,Hohng S

更新日期：2018-01-09 00:00:00

abstract：:Dopaminergic and glutamatergic neurotransmissions in the striatum play an essential role in motor- and reward-related behaviors. Dysfunction of these neurotransmitter systems has been found in Parkinson's disease, schizophrenia, and drug addiction. Cyclin-dependent kinase 5 (CDK5) negatively regulates postsynaptic sig...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chergui K,Svenningsson P,Greengard P

更新日期：2004-02-17 00:00:00

abstract：:The embryonic development of the optic nerve of the zebrafish, Brachydanio rerio, was studied by three-dimensional computer reconstruction from serial section electron micrographs. Growing fibers from retinal ganglion cells had growth cones in contact with more mature fibers from adjacent cell bodies. In the observed ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bodick N,Levinthal C

更新日期：1980-07-01 00:00:00

abstract：:Dihydrofolate reductase (EC 1.5.1.3; 5,6,7,8-tetrahydrofolate:NADP(+) oxidoreductase) from antifolate-resistant Lactobacillus casei has been isolated in pure form and examined in solution by high resolution proton magnetic resonance spectroscopy. The 220 MHz proton magnetic resonance spectrum of this small enzyme (abo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pastore EJ,Kisliuk RL,Plante LT,Wright JM,Kaplan NO

更新日期：1974-10-01 00:00:00

abstract：:A low molecular weight inhibitor of cell-free protein synthesis effective at subnanomolar concentrations is formed on incubation of cytoplasmic extracts from interferon-treated cells with double-stranded RNA and ATP. It can be conveniently synthesized by incubating a poly(I).poly(C)-Sepharose-bound enzyme fraction fro...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kerr IM,Brown RE

更新日期：1978-01-01 00:00:00

abstract：:The basic theory of a newly discovered physical technique, steady-state electrophoresis, is described followed by analyses of several representative macro-ion systems with the technique. This method measures apparent moments of molar diminished charge (defined below) of solvated macro-ions from an analysis of a concen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Godfrey JE

更新日期：1989-06-01 00:00:00

abstract：:The synthesis of the proteins laminin and collagen IV is stimulated approximately equal to 20-fold in F9 mouse teratocarcinoma stem cells after treatment of the cells with retinoic acid and N6, O2'-dibutyryl-cAMP (Bt2cAMP). A cDNA library from F9 cells treated with retinoic acid, Bt2cAMP, and theophylline (F9-R + DBC ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang SY,Gudas LJ

更新日期：1983-10-01 00:00:00

abstract：:The developmental origin of dendritic cells, a specialized system of major histocompatibility complex (MHC) class II-rich antigen-presenting cells for T-cell immunity and tolerance, is not well characterized. Granulocyte-macrophage colony-stimulating factor (GM-CSF) is known to stimulate dendritic cells, including gro...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Inaba K,Inaba M,Deguchi M,Hagi K,Yasumizu R,Ikehara S,Muramatsu S,Steinman RM

更新日期：1993-04-01 00:00:00

abstract：:Glc3Man9GlcNAc2 is the preferred substrate of the oligosaccharyltransferase of N-linked glycosylation of proteins, but nonglucosylated oligosaccharides can be transferred to proteins in Saccharomyces cerevisiae. Mutations affecting the addition of the three terminal glucose residues lead to accumulation of Man9GlcNAc2...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stagljar I,te Heesen S,Aebi M

更新日期：1994-06-21 00:00:00

abstract：:Tau is a highly soluble protein, yet it aggregates abnormally in Alzheimer's disease. Here, we address the question of proteolytic processing of tau and the nucleation of aggregates by tau fragments. We show in neuronal cell models that fragments of the repeat domain of tau containing mutations of FTDP17 (frontotempor...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang YP,Biernat J,Pickhardt M,Mandelkow E,Mandelkow EM

更新日期：2007-06-12 00:00:00

abstract：:In this article, a mechanism of arsenite [As(III)]resistance through methylation and subsequent volatization is described. Heterologous expression of arsM from Rhodopseudomonas palustris was shown to confer As(III) resistance to an arsenic-sensitive strain of Escherichia coli. ArsM catalyzes the formation of a number ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Qin J,Rosen BP,Zhang Y,Wang G,Franke S,Rensing C

更新日期：2006-02-14 00:00:00

abstract：:Eosinophil-derived neurotoxin (EDN) and eosinophil cationic protein (ECP) were isolated from lysates of human eosinophil granules by gel filtration and ion exchange chromatography on heparin-Sepharose. Radioimmunoassay, using monoclonal antibodies, of fractions from the heparin-Sepharose chromatography showed one peak...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gleich GJ,Loegering DA,Bell MP,Checkel JL,Ackerman SJ,McKean DJ

更新日期：1986-05-01 00:00:00

abstract：:We describe a procedure for predicting the tertiary folds of alpha-helical proteins from their primary sequences. The central component of the procedure is a method for predicting interhelical contacts that is based on a helix-packing model. Instead of predicting the individual contacts, our method attempts to identif...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhang C,Hou J,Kim SH

更新日期：2002-03-19 00:00:00

abstract：:The basic assumptions of van der Waals theory are contained in two well-known concepts: excluded volume (repulsive forces) and a homogeneous, isotropic field potential (attractive forces). We have superimposed on these, one more well-known concept: the existence of dimers, trimers, etc., at chemical equilibrium. With ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Heidemann RA,Prausnitz JM

更新日期：1976-06-01 00:00:00

abstract：:An intact T/E1A-binding domain (the pocket) is necessary, but not sufficient, for the retinoblastoma protein (RB) to bind to DNA-protein complexes containing E2F and for RB to induce a G1/S block. Indirect evidence suggests that the binding of RB to E2F may, in addition to inhibiting E2F transactivation function, gene...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sellers WR,Rodgers JW,Kaelin WG Jr

更新日期：1995-12-05 00:00:00

abstract：:Seed development in plants involves the coordinated growth of the embryo, endosperm, and maternal tissue. Several genes have been identified that influence seed size by acting maternally, such as AUXIN RESPONSE FACTOR2, APETALA2, and DA1. However, given the lack of gain-of-function effects of these genes on seed size,...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Adamski NM,Anastasiou E,Eriksson S,O'Neill CM,Lenhard M

更新日期：2009-11-24 00:00:00