Brain G protein gamma subunits contain an all-trans-geranylgeranylcysteine methyl ester at their carboxyl termini.

abstract：:The auditory cortex is critical for perceiving a sound's location. However, there is no topographic representation of acoustic space, and individual auditory cortical neurons are often broadly tuned to stimulus location. It thus remains unclear how acoustic space is represented in the mammalian cerebral cortex and how...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miller LM,Recanzone GH

更新日期：2009-04-07 00:00:00

abstract：:How do firing patterns in a cortical circuit change when inhibitory neurons are excited? We virally expressed an excitatory designer receptor exclusively activated by a designer drug (Gq-DREADD) in all inhibitory interneuron types of the CA1 region of the hippocampus in the rat. While clozapine N-oxide (CNO) activatio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rogers S,Rozman PA,Valero M,Doyle WK,Buzsáki G

更新日期：2021-01-12 00:00:00

abstract：:We have detected an endoglycosidase activity produced by Flavobacterium meningosepticum. This enzyme, named endo F, cleaves glycans of both the high-mannose and the complex type linked through asparagine to the protein backbone. The data indicate that cleavage occurs via hydrolysis of the glycosidic bond of the N,N'-d...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Elder JH,Alexander S

更新日期：1982-08-01 00:00:00

abstract：:Chronic inflammation results in increased nitrogen monoxide (.NO) formation and the accumulation of nitrite (NO(2-)). Neutrophils stimulated by various inflammatory mediators release myeloperoxidase to produce the cytotoxic agent hypochlorous acid (HOCl). Exposure of chondrocytic SW1353 cells to HOCl resulted in a con...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Whiteman M,Hooper DC,Scott GS,Koprowski H,Halliwell B

更新日期：2002-09-17 00:00:00

abstract：:In order to understand the functions of simian virus 40 genes, permissive cells (TC7) were infected with mutants temperature sensitive in the complementation groups A, B, C, BC, and D at permissive and nonpermissive temperatures. Cells were examined for the localization of viral polypeptide antigens by immunofluoresce...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kasamatsu H,Nehorayan A

更新日期：1979-06-01 00:00:00

abstract：:Perception can be described as a process of inference, integrating bottom-up sensory inputs and top-down expectations. However, it is unclear how this process is neurally implemented. It has been proposed that expectations lead to prestimulus baseline increases in sensory neurons tuned to the expected stimulus, which ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kok P,Mostert P,de Lange FP

更新日期：2017-09-26 00:00:00

abstract：:The degradation of the plant cell wall by glycoside hydrolases is central to environmentally sustainable industries. The major polysaccharides of the plant cell wall are cellulose and xylan, a highly decorated β-1,4-xylopyranose polymer. Glycoside hydrolases displaying multiple catalytic functions may simplify the enz...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McKee LS,Peña MJ,Rogowski A,Jackson A,Lewis RJ,York WS,Krogh KB,Viksø-Nielsen A,Skjøt M,Gilbert HJ,Marles-Wright J

更新日期：2012-04-24 00:00:00

abstract：:In this paper, Vafa's approach to the formulation of conformal field theories is combined with the formal calculus developed in Frenkel, Lepowsky, and Meurman's work on the vertex operator construction of the Monster to give a geometric definition of vertex operator algebras. The main result announced is the equivalen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Huang YZ

更新日期：1991-11-15 00:00:00

abstract：:Phage phi 29 DNA cannot be phosphorylated with polynucleotide kinase and [gamma-32P]ATP because of the presence of a viral protein covalently linked to the 5' termini. The 5' ends can, however, be made susceptible to phosphorylation by treatment with alkali and alkaline phosphatase. Restriction fragments Hpa II C and ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Escarmís C,Salas M

更新日期：1981-03-01 00:00:00

abstract：:Translation of injected mRNA in oocytes of Xenopus laevis has been used as a highly sensitive and quantitative assay for interferon mRNA. Injection into oocytes of polyadenylylated RNA extracted from poly(I).poly(C)-induced human diploid fibroblasts (FS-4) leads to the synthesis of biologically active human fibroblast...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sehgal PB,Dobberstein B,Tamm I

更新日期：1977-08-01 00:00:00

abstract：:An epithelial cell line, designated COMMA-1D, was derived from mammary tissue of BALB/c mice in the middle of pregnancy. This line, in continuous cell culture for 12 months, exhibits several characteristics distinctive of normal mammary epithelial cells, including induction of casein synthesis in vitro and normal duct...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Danielson KG,Oborn CJ,Durban EM,Butel JS,Medina D

更新日期：1984-06-01 00:00:00

abstract：:Gyrate atrophy (GA), a recessive eye disease involving progressive loss of vision due to chorioretinal degeneration, is associated with a deficiency of the mitochondrial enzyme ornithine aminotransferase (OATase; ornithine-oxo-acid aminotransferase; L-ornithine:2-oxo-acid aminotransferase, EC 2.6.1.13) with consequent...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ramesh V,McClatchey AI,Ramesh N,Benoit LA,Berson EL,Shih VE,Gusella JF

更新日期：1988-06-01 00:00:00

abstract：:ClpB is a ring-forming, ATP-dependent protein disaggregase that cooperates with the cognate Hsp70 system to recover functional protein from aggregates. How ClpB harnesses the energy of ATP binding and hydrolysis to facilitate the mechanical unfolding of previously aggregated, stress-damaged proteins remains unclear. H...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Biter AB,Lee S,Sung N,Tsai FT

更新日期：2012-07-31 00:00:00

abstract：:Bacterial outer membrane porins have a robust beta-barrel structure and therefore show potential for use as stochastic sensors based on single-molecule detection. The monomeric porin OmpG is especially attractive compared with multisubunit proteins because appropriate modifications of the pore can be easily achieved b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen M,Khalid S,Sansom MS,Bayley H

更新日期：2008-04-29 00:00:00

abstract：:To clone the gene for the human Na+/H+ antiporter, we first constructed a stable mouse LTK- cell line (LAP1) lacking Na+/H+ antiport activity. Second, we devised a selective technique based on acid killing that specifically sorts out cells expressing low levels of Na+/H+ antiport activity from a population of antiport...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Franchi A,Perucca-Lostanlen D,Pouyssegur J

更新日期：1986-12-01 00:00:00

abstract：:The mitochondrial DNA polymerase from Drosophila embryos lacks dNTP turnover activity. However, a potent 3'----5' exonuclease activity can be detected by a specific assay in which the exonuclease excises mispaired nucleotides at the 3' termini of primed synthetic and natural DNA templates. The excision of a mispaired ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kaguni LS,Olson MW

更新日期：1989-09-01 00:00:00

abstract：:Human lymphocytes obtained from fasted adult subjects and cultured human tumor lymphocytes were investigated for specific insulin receptors. By use of monoiodoinsulin, specific insulin binding sites were demonstrated in peripheral human lymphocytes, cultured human lymphocytes, and in other types of human circulating c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gavin JR 3rd,Roth J,Jen P,Freychet P

更新日期：1972-03-01 00:00:00

abstract：:We have inserted the sequence 5'-AAG-GAGGU-3', which is complementary to the 3' terminus of Escherichia coli 16S rRNA, in a reading frame and analyzed its effect on the accuracy and overall rate of translation in vivo. Translation over the sequence yields a 50% ribosomal frameshift if the reading phase is A-AGG-AGG-U....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Spanjaard RA,van Duin J

更新日期：1988-11-01 00:00:00

abstract：:The cell-surface antigen detected by the monoclonal antibody JONES is expressed in the retina and a number of other central nervous system regions of the rat during the latter part of embryonic development and the early postnatal period. In addition to the expression on certain neuroblast populations it is found on so...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Blum AS,Barnstable CJ

更新日期：1987-12-01 00:00:00

abstract：:The final step in hydrocarbon biosynthesis involves loss of CO from a fatty aldehyde. This decarbonylation is catalyzed by microsomes from Botyrococcus braunii. Among the several detergents tested for solubilizing the decarbonylase, octyl beta-glucoside (0.1%) was found to be the most effective and released 65% of the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dennis M,Kolattukudy PE

更新日期：1992-06-15 00:00:00

abstract：:Liddle syndrome is a mendelian form of hypertension characterized by constitutively elevated renal Na reabsorption that can result from activating mutations in the beta or gamma subunit of the epithelial Na channel. All reported mutations have deleted the last 45-76 normal amino acids from the cytoplasmic C terminus o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hansson JH,Schild L,Lu Y,Wilson TA,Gautschi I,Shimkets R,Nelson-Williams C,Rossier BC,Lifton RP

更新日期：1995-12-05 00:00:00

abstract：:The biosynthesis of beta-nerve growth factor (betaNGF) was studied in mouse submaxillary glands incubated with L-[(35)S]cystine. betaNGF was isolated from tissue extracts by the addition of antiserum against betaNGF and the washed immunoprecipitates were analyzed by sodium dodecyl sulfate gel electrophoresis. With sho...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Berger EA,Shooter EM

更新日期：1977-09-01 00:00:00

abstract：:The harvest of wildlife for human consumption is valued at several billion dollars annually and provides an essential source of meat for hundreds of millions of rural people living in poverty. This harvest is also considered among the greatest threats to biodiversity throughout Africa, Asia, and Latin America. Economi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brashares JS,Golden CD,Weinbaum KZ,Barrett CB,Okello GV

更新日期：2011-08-23 00:00:00

abstract：:Neovascular diseases of the retina include age-related macular degeneration and diabetic retinopathy, and together they comprise the leading causes of adult-onset blindness in developed countries. Current surgical, pharmaceutical, and laser therapies for age-related macular degeneration (AMD) rarely result in improved...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Raisler BJ,Berns KI,Grant MB,Beliaev D,Hauswirth WW

更新日期：2002-06-25 00:00:00

abstract：:Cytological preparations of interphase nuclei and chromosomes from mouse 3T6 cells prepared at various times after infection with the murine sarcomaleukemia virus complex were hybridized with the [(3)H]DNA product of the viral RNA-directed DNA polymerase. While uninfected nuclei had an average of 4 autoradiographic gr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Loni MC,Green M

更新日期：1974-09-01 00:00:00

abstract：:During zebrafish development, zygotic gene expression initiated at the midblastula transition converts maternal information on embryo polarity into a transcriptional read-out. Expression of a homeobox gene, vega1, is activated at midblastula transition in all blastomeres, but is down-regulated dorsally before gastrula...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kawahara A,Wilm T,Solnica-Krezel L,Dawid IB

更新日期：2000-10-24 00:00:00

abstract：:The recent discovery of superconductivity with relatively high transition temperature (Tc) in the layered iron-based quaternary oxypnictides La[O(1-x)F(x)] FeAs by Kamihara et al. [Kamihara Y, Watanabe T, Hirano M, Hosono H (2008) Iron-based layered superconductor La[O1-xFx] FeAs (x = 0.05-0.12) with Tc = 26 K. J Am C...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hsu FC,Luo JY,Yeh KW,Chen TK,Huang TW,Wu PM,Lee YC,Huang YL,Chu YY,Yan DC,Wu MK

更新日期：2008-09-23 00:00:00

abstract：:The objective of this study has been to determine which Na,K-ATPase isoforms are expressed in red blood cells and whether kinetic differences in the uncoupled sodium efflux mode between the human red blood cell Na,K-ATPase and other preparations can be explained by differences in the underlying subunit composition. To...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stengelin MK,Hoffman JF

更新日期：1997-05-27 00:00:00

abstract：:The avian predentary is a small skeletal structure located rostral to the paired dentaries found only in Mesozoic ornithuromorphs. The evolution and function of this enigmatic element is unknown. Skeletal tissues forming the predentary and the lower jaws in the basal ornithuromorph Yanornis martini are identified usin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bailleul AM,Li Z,O'Connor J,Zhou Z

更新日期：2019-12-03 00:00:00

abstract：:Learning theories hypothesize specific circuits encode essential information for performance. For simple tasks in invertebrates and mammals, the essential circuits are known, but for cognitive functions, the essential circuits remain unidentified. Here, we show that some essential information for performing a choice t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhang GR,Cao H,Kong L,O'Brien J,Baughns A,Jan M,Zhao H,Wang X,Lu XG,Cook RG,Geller AI

更新日期：2010-08-10 00:00:00