Nucleotide sequence at the termini of the DNA of Bacillus subtilis phage phi 29.

abstract：:Intestinal trefoil factor (ITF) is an essential regulator of colonic epithelial restitution, the rapid migration of colonocytes over mucosal wounds. High levels of ITF are frequently present in colorectal cancers and derived cell lines. Mucosal restitution requires the detachment of epithelium from substrate, which wo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Taupin DR,Kinoshita K,Podolsky DK

更新日期：2000-01-18 00:00:00

abstract：:The interactions between products of the 15-lipoxygenase cascade and human natural killer (NK) cell activity have been studied. Addition of human leukocyte-derived (14R,15S)-14,15-dihydroxy-5Z,8Z,10E,12E-ic osatetraenoic acid (14,15-DiHETE) to the NK cytotoxicity assay against K562 target cells resulted in inhibition ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ramstedt U,Serhan CN,Lundberg U,Wigzell H,Samuelsson B

更新日期：1984-11-01 00:00:00

abstract：:We report a machine-learning strategy for design of organic structure directing agents (OSDAs) for zeolite beta. We use machine learning to replace a computationally expensive molecular dynamics evaluation of the stabilization energy of the OSDA inside zeolite beta with a neural network prediction. We train the neural...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Daeyaert F,Ye F,Deem MW

更新日期：2019-02-26 00:00:00

abstract：:Starving Bacillus subtilis cells execute a gene expression program resulting in the formation of stress-resistant spores. Sporulation master regulator, Spo0A, is activated by a phosphorelay and controls the expression of a multitude of genes, including the forespore-specific sigma factor σ(F) and the mother cell-speci...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Narula J,Devi SN,Fujita M,Igoshin OA

更新日期：2012-12-11 00:00:00

abstract：:Genetic switches based on the [Formula: see text] system are master regulators of an array of cellular responses. Recent kinetic experiments have shown that [Formula: see text] can actively remove NF-κB bound to its genetic sites via a process called "molecular stripping." This allows the [Formula: see text] switch to...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Potoyan DA,Zheng W,Komives EA,Wolynes PG

更新日期：2016-01-05 00:00:00

abstract：:Gut microbial composition depends on different dietary habits just as health depends on microbial metabolism, but the association of microbiota with different diets in human populations has not yet been shown. In this work, we compared the fecal microbiota of European children (EU) and that of children from a rural Af...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： De Filippo C,Cavalieri D,Di Paola M,Ramazzotti M,Poullet JB,Massart S,Collini S,Pieraccini G,Lionetti P

更新日期：2010-08-17 00:00:00

abstract：:Although it is well described in model membranes, little is known about phase separation in biological membranes. Here, we provide evidence for a coexistence of at least two different lipid bilayer phases in the apical plasma membrane of epithelial cells. Phase connectivity was assessed by measuring long-range diffusi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Meder D,Moreno MJ,Verkade P,Vaz WL,Simons K

更新日期：2006-01-10 00:00:00

abstract：:The primary product of biological nitrogen fixation, ammonia, reversibly regulates nitrogenase activity in a variety of diazotrophs by a process called "NH4(+)-switch-off/on." Strong correlative evidence from work in Azospirillum lipoferum and Rhodospirillum rubrum indicates that this regulation involves both the inac...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fu H,Burris RH,Roberts GP

更新日期：1990-03-01 00:00:00

abstract：:Extensive terahertz laser vibration-rotation-tunneling spectra and mid-IR laser spectra have been compiled for several isotopomers of small (dimer through hexamer) water clusters. These data, in conjunction with new theoretical advances, quantify the structures, force fields, dipole moments, and hydrogen bond rearrang...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Keutsch FN,Saykally RJ

更新日期：2001-09-11 00:00:00

abstract：:The spectrum of cellular targets in the autoimmune diseases is both large and varied and includes among the nuclear components the so-called Sm and RNP antigens associated with systemic lupus erythematosus. The use of immunoaffinity chromatography with dual specificity for the Sm and RNP antigens has allowed for their...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： White PJ,Gardner WD,Hoch SO

更新日期：1981-01-01 00:00:00

abstract：:W. D. Hamilton's celebrated formula for the age-specific force of natural selection furnishes predictions for senescent mortality due to mutation accumulation, at the price of reliance on a linear approximation. Applying to Hamilton's setting the full nonlinear demographic model for mutation accumulation recently deve...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wachter KW,Evans SN,Steinsaltz D

更新日期：2013-06-18 00:00:00

abstract：:Compensatory ventilatory responses to increased inspiratory loading are essential for adequate breathing regulation in a number of pulmonary diseases; however, the human brain sites mediating such responses are unknown. Midsagittal and axial images were acquired in 11 healthy volunteers during unloaded and loaded (30 ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gozal D,Omidvar O,Kirlew KA,Hathout GM,Hamilton R,Lufkin RB,Harper RM

更新日期：1995-07-03 00:00:00

abstract：:A direct method for the determination of N-linked glycosylation sites in highly glycosylated proteins is described. Carcinoembryonic antigen (CEA) and a nonspecific crossreacting antigen (NCA) were chemically deglycosylated, and peptide maps were prepared by reverse-phase HPLC. The peptides were sequenced on a gas-pha...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Paxton RJ,Mooser G,Pande H,Lee TD,Shively JE

更新日期：1987-02-01 00:00:00

abstract：:Most cells in the well-known pre-B-lymphocyte line 18-81 have correctly assembled genes for both alleles at the immunoglobulin heavy chain locus. Only one allele is "active"; the other, "silent" allele contains an amber termination codon. The rate of reversion of this amber codon was determined to be 0.3-1 X 10(-4) pe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wabl M,Burrows PD,von Gabain A,Steinberg C

更新日期：1985-01-01 00:00:00

abstract：:Cytotoxic T lymphocytes (CTLs) recognize antigens in the context of major histocompatibility complex (MHC) class I gene products. The T-cell receptor (TCR) that mediates this MHC-restricted antigen recognition recognizes short peptide fragments rather than the intact antigen. Presentation of peptides to the TCR may th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kievits F,Wijffels J,Lokhorst W,Ivanyi P

更新日期：1989-01-01 00:00:00

abstract：:We have cloned and characterized cDNAs coding for a receptor-class phosphotyrosine phosphatase gene from Drosophila melanogaster. The gene maps to the polytene chromosome bands 99A7-8. The cDNA clones code for a polypeptide of 1301 amino acids with a predicted molecular mass of 145 kDa. The extracellular domain includ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hariharan IK,Chuang PT,Rubin GM

更新日期：1991-12-15 00:00:00

abstract：:Von Willebrand factor-binding protein (VWbp), secreted by Staphylococcus aureus, displays secondary structural homology to the 3-helix bundle, D1 and D2 domains of staphylocoagulase (SC), a potent conformational activator of the blood coagulation zymogen, prothrombin (ProT). In contrast to the classical proteolytic ac...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kroh HK,Panizzi P,Bock PE

更新日期：2009-05-12 00:00:00

abstract：:Cell cultures of Catharanthus roseus were supplied with [2-13C, 3-2H]-deoxyxylulose or [2-13C,4-2H]1-deoxyxylulose. Lutein and chlorophylls were isolated from the cell mass, and hydrolysis of the chlorophyll mixtures afforded phytol. Isotope labeling patterns of phytol and lutein were determined by 2H NMR and 1H,2H-de...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Arigoni D,Eisenreich W,Latzel C,Sagner S,Radykewicz T,Zenk MH,Bacher A

更新日期：1999-02-16 00:00:00

abstract：:The sea anemone, Nematostella vectensis, has become an attractive new model organism for comparative genomics and evolutionary developmental biology. Over the last few years, many genes have been isolated and their expression patterns studied to gain insight into their function. More recently, functional tools have be...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Renfer E,Amon-Hassenzahl A,Steinmetz PR,Technau U

更新日期：2010-01-05 00:00:00

abstract：:Larger brains have an increasingly folded cerebral cortex whose white matter scales up faster than the gray matter. Here we analyze the cellular composition of the subcortical white matter in 11 primate species, including humans, and one Scandentia, and show that the mass of the white matter scales linearly across spe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Herculano-Houzel S,Mota B,Wong P,Kaas JH

更新日期：2010-11-02 00:00:00

abstract：:Most guanine nucleotide binding protein (G protein)-coupled receptors have a conserved cysteine in the C-terminal cytoplasmic tail near the seventh transmembrane spanning region. This cysteine is known to be palmitoylated in rhodopsin, the beta 2-adrenergic receptor (beta 2AR) and the alpha 2A-adrenergic receptor (alp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Eason MG,Jacinto MT,Theiss CT,Liggett SB

更新日期：1994-11-08 00:00:00

abstract：:Respiratory cytochrome c has been found to be phosphorylated at tyrosine 97 in the postischemic brain upon neuroprotective insulin treatment, but how such posttranslational modification affects mitochondrial metabolism is unclear. Here, we report the structural features and functional behavior of a phosphomimetic cyto...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Guerra-Castellano A,Díaz-Quintana A,Pérez-Mejías G,Elena-Real CA,González-Arzola K,García-Mauriño SM,De la Rosa MA,Díaz-Moreno I

更新日期：2018-07-31 00:00:00

abstract：:The tau and gamma subunits of DNA polymerase III holoenzyme are both products of the dnaX gene. Since tau and gamma are required as stoichiometric components of the replicative complex, a mechanism must exist for the cell to coordinate their synthesis and ensure that both subunits are present in an adequate quantity a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Flower AM,McHenry CS

更新日期：1990-05-01 00:00:00

abstract：:Mutations at the mouse limb deformity (ld) locus result in defects of growth and patterning of the limb and kidney during embryonic development. The gene responsible for this phenotype is large and complex, with the capacity to generate a number of alternatively spliced messenger RNA transcripts encoding nuclear prote...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vogt TF,Jackson-Grusby L,Rush J,Leder P

更新日期：1993-06-15 00:00:00

abstract：:Heat shock locus V (HslV; also called ClpQ) is the proteolytic core of the ATP-dependent protease HslVU in Escherichia coli. It has sequence similarity with the beta-type subunits of the eukaryotic and archaebacterial proteasomes. Unlike these particles, which display 72-point symmetry, it is a dimer of hexamers with ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bochtler M,Ditzel L,Groll M,Huber R

更新日期：1997-06-10 00:00:00

abstract：:Investigation of retinal neurochemistry in a well-defined chick model of form-deprivation myopia indicated that dopamine and its metabolite 3,4-dihydroxyphenylacetic acid are reduced in myopic as compared to control eyes. The reduction in retinal dopamine is evident only during light adaptation and is accompanied by a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stone RA,Lin T,Laties AM,Iuvone PM

更新日期：1989-01-01 00:00:00

abstract：:A key element for the development of suitable anti-cancer drugs is the identification of cancer-specific enzymatic activities that can be therapeutically targeted. Mucosa-associated lymphoid tissue transformation protein 1 (MALT1) is a proto-oncogene that contributes to tumorigenesis in diffuse large B-cell lymphoma (...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hailfinger S,Lenz G,Ngo V,Posvitz-Fejfar A,Rebeaud F,Guzzardi M,Penas EM,Dierlamm J,Chan WC,Staudt LM,Thome M

更新日期：2009-11-24 00:00:00

abstract：:Antibodies prepared against peptides CP2, CP4, and CP5, which occur within the first 1522 amino acid residues of the alpha 1 subunit of dihydropyridine-sensitive skeletal muscle calcium channels, specifically recognized a 175-kDa form of the alpha 1 subunit in immunoblots and immunoprecipitation experiments. In contra...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： De Jongh KS,Merrick DK,Catterall WA

更新日期：1989-11-01 00:00:00

abstract：:Cytoplasmic RNA extracted from human tissue culture cells infected with adenovirus type 2 was used to program protein synthesis in a cell-free system derived from mammalian cells. Analysis of the protein product by polyacrylamide gel electrophoresis revealed ten adenovirus-specific polypeptides. Five of these were fur...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Anderson CW,Lewis JB,Atkins JF,Gesteland RF

更新日期：1974-07-01 00:00:00

abstract：:Translational initiation factor 2 (IF2) is a guanine nucleotide-binding protein that can bind guanosine 3',5'-(bis) diphosphate (ppGpp), an alarmone involved in stringent response in bacteria. In cells growing under optimal conditions, the GTP concentration is very high, and that of ppGpp very low. However, under stre...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Milon P,Tischenko E,Tomsic J,Caserta E,Folkers G,La Teana A,Rodnina MV,Pon CL,Boelens R,Gualerzi CO

更新日期：2006-09-19 00:00:00