Abstract:
:Column chromatography on (dT)(12-18)-cellulose was used to detect and isolate a novel DNA polymerase activity from human breast tumor cell line HBT-3. The new enzyme is similar to those of mammalian RNA tumor viruses in its molecular size, preference for poly(A).(dT)(12-18) as compared to either poly(dA).(dT)(12-18) or poly(A).poly(dT), and ability to copy poly(C).(dG)(12-18). A similar DNA polymerase activity, defined by its binding to (dT)(12-18)-cellulose, is not detectable in several other cell lines of human tumors.
journal_name
Proc Natl Acad Sci U S Aauthors
Gerwin BI,Bassin RHdoi
10.1073/pnas.70.8.2453subject
Has Abstractpub_date
1973-08-01 00:00:00pages
2453-6issue
8eissn
0027-8424issn
1091-6490journal_volume
70pub_type
杂志文章abstract::To understand complex biological systems, such as the development of multicellular organisms, it is important to characterize the gene expression dynamics. However, there is currently no universal technique for targeted insertion of reporter genes and quantitative imaging in multicellular model systems. Recently, geno...
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