Lipid vesicles as carriers for introducing materials into cultured cells: influence of vesicle lipid composition on mechanism(s) of vesicle incorporation into cells.

abstract：:Muscular dystrophy is a general term encompassing muscle disorders that cause weakness and wasting, typically leading to premature death. Membrane instability, as a result of a genetic disruption within the dystrophin-glycoprotein complex (DGC), is thought to induce myofiber degeneration, although the downstream mecha...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Millay DP,Goonasekera SA,Sargent MA,Maillet M,Aronow BJ,Molkentin JD

更新日期：2009-11-10 00:00:00

abstract：:Under natural viewing conditions the input to the retina is a complex spatiotemporal signal that depends on both the scene and the way the observer moves. It is commonly assumed that the retina processes this input signal efficiently by taking into account the statistics of the natural world. It has recently been argu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Segal IY,Giladi C,Gedalin M,Rucci M,Ben-Tov M,Kushinsky Y,Mokeichev A,Segev R

更新日期：2015-03-10 00:00:00

abstract：:Mesenteric fat is known to undergo inflammatory changes after 2,4,6,-trinitrobenzensulphonic acid (TNBS)-induced colitis. Neurotensin (NT) and neurotensin receptor 1 (NTR1) have been shown to play a major role in the pathogenesis of intestinal inflammation. This led us to explore whether NT and NTR1 are expressed in t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Koon HW,Kim YS,Xu H,Kumar A,Zhao D,Karagiannides I,Dobner PR,Pothoulakis C

更新日期：2009-05-26 00:00:00

abstract：:Despite intense investigation over the past century, the molecular mechanisms that regulate maintenance and adaptation of the heart during postnatal development are poorly understood. Myocardin is a remarkably potent transcriptional coactivator expressed exclusively in cardiac myocytes and smooth muscle cells during p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Huang J,Min Lu M,Cheng L,Yuan LJ,Zhu X,Stout AL,Chen M,Li J,Parmacek MS

更新日期：2009-11-03 00:00:00

abstract：:The formation of several acyl groups and an amide group of Taxol is catalyzed by regioselective CoA thioester-dependent acyltransferases. Several full-length acyltransferase sequences, obtained from a cDNA library constructed from mRNA isolated from Taxus cuspidata cells induced for Taxol production with methyl jasmon...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Walker K,Long R,Croteau R

更新日期：2002-07-09 00:00:00

abstract：:G proteins are activated when they associate with G protein-coupled receptors (GPCRs), often in response to agonist-mediated receptor activation. It is generally thought that agonist-induced receptor-G protein association necessarily promotes G protein activation and, conversely, that activated GPCRs do not interact w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Okashah N,Wright SC,Kawakami K,Mathiasen S,Zhou J,Lu S,Javitch JA,Inoue A,Bouvier M,Lambert NA

更新日期：2020-09-01 00:00:00

abstract：:Metallo-β-lactamases (MBLs) hydrolyze almost all β-lactam antibiotics and are unaffected by clinically available β-lactamase inhibitors (βLIs). Active-site architecture divides MBLs into three classes (B1, B2, and B3), complicating development of βLIs effective against all enzymes. Bisthiazolidines (BTZs) are carboxyl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hinchliffe P,González MM,Mojica MF,González JM,Castillo V,Saiz C,Kosmopoulou M,Tooke CL,Llarrull LI,Mahler G,Bonomo RA,Vila AJ,Spencer J

更新日期：2016-06-28 00:00:00

abstract：:Phytoplankton often encounter turbulence in their habitat. As most toxic phytoplankton species are motile, resolving the interplay of motility and turbulence has fundamental repercussions on our understanding of their own ecology and of the entire ecosystems they inhabit. The spatial distribution of motile phytoplankt...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Breier RE,Lalescu CC,Waas D,Wilczek M,Mazza MG

更新日期：2018-11-27 00:00:00

abstract：:The chemokine stromal cell-derived factor 1, SDF-1, is an important regulator of leukocyte and hematopoietic precursor migration and pre-B cell proliferation. The receptor for SDF-1, CXCR4, also functions as a coreceptor for T-tropic HIV-1 entry. We find that mice deficient for CXCR4 die perinatally and display profou...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ma Q,Jones D,Borghesani PR,Segal RA,Nagasawa T,Kishimoto T,Bronson RT,Springer TA

更新日期：1998-08-04 00:00:00

abstract：:Biodistribution studies of a water-soluble radioactive metallofullerene compound have been conducted using BALB/c mice. To this end, a sample containing Hox@C82 (x = 1, 2) was purified and derivatized to prepare the water-soluble metallofullerol, Hox@C82(OH)y. This metallofullerol was then neutron-activated (165Ho[n,g...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cagle DW,Kennel SJ,Mirzadeh S,Alford JM,Wilson LJ

更新日期：1999-04-27 00:00:00

abstract：:Pseudohypoparathyroidism type Ia (PHP-Ia) results from the loss of one allele of G(salpha), causing resistance to parathyroid hormone and other hormones that transduce signals via G(s). Most G(salpha)mutations cause the complete loss of protein expression, but some cause loss of function only, and these have provided ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Makita N,Sato J,Rondard P,Fukamachi H,Yuasa Y,Aldred MA,Hashimoto M,Fujita T,Iiri T

更新日期：2007-10-30 00:00:00

abstract：:A stable complex between pentaammineruthenium(III) and histidine-33 in horse heart ferricytochrome c is formed in the reaction between aquopentaammineruthenium(II) and the protein at pH 7. HPLC of the tryptic hydrolysate of the modified protein was employed to identify the pentaammineruthenium binding site. Spectrosco...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yocom KM,Shelton JB,Shelton JR,Schroeder WA,Worosila G,Isied SS,Bordignon E,Gray HB

更新日期：1982-11-01 00:00:00

abstract：:One half of a group of 20 patients with human papillomavirus type 16 (HPV16)-induced vulvar intraepithelial neoplasia grade 3 displayed a complete regression (CR) after therapeutic vaccination with HPV16 E6/E7 synthetic long peptides. Patients with relatively larger lesions generally did not display a CR. To investiga...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Welters MJ,Kenter GG,de Vos van Steenwijk PJ,Löwik MJ,Berends-van der Meer DM,Essahsah F,Stynenbosch LF,Vloon AP,Ramwadhdoebe TH,Piersma SJ,van der Hulst JM,Valentijn AR,Fathers LM,Drijfhout JW,Franken KL,Oostendorp J,Fle

更新日期：2010-06-29 00:00:00

abstract：:The Hippo-YAP/TAZ signaling pathway plays a pivotal role in growth control during development and regeneration and its dysregulation is widely implicated in various cancers. To further understand the cellular and molecular mechanisms underlying Hippo signaling regulation, we have found that activities of core Hippo si...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kim W,Cho YS,Wang X,Park O,Ma X,Kim H,Gan W,Jho EH,Cha B,Jeung YJ,Zhang L,Gao B,Wei W,Jiang J,Chung KS,Yang Y

更新日期：2019-05-07 00:00:00

abstract：:Swimming Escherichia coli responds to changes in temperature by modifying its motor behavior. Previous studies using populations of cells have shown that E. coli accumulate in spatial thermal gradients, but these experiments did not cleanly separate thermal responses from chemotactic responses. Here we have isolated t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Paster E,Ryu WS

更新日期：2008-04-08 00:00:00

abstract：:A central question in protein-DNA recognition is the origin of the specificity that permits binding to the correct site in the presence of excess, nonspecific DNA. In the P22 Arc repressor, the Phe-10 side chain is part of the hydrophobic core of the free protein but rotates out to pack against the sugar-phosphate bac...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schildbach JF,Karzai AW,Raumann BE,Sauer RT

更新日期：1999-02-02 00:00:00

abstract：:Proteolytic removal of the pre-segment from growing nascent chains of pre-human placental lactogen (hPL) occurred during in vitro translation of placental mRNA if crude membranes derived from ascites lysates, dog pancreas, or rat liver rough endoplasmic reticulum were added to the translation mixtures. The cotranslati...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Strauss AW,Zimmerman M,Boime I,Ashe B,Mumford RA,Alberts AW

更新日期：1979-09-01 00:00:00

abstract：:The spatial distribution of plant defenses within a leaf may be critical in explaining patterns of herbivory. The generalist lepidopteran larvae, Helicoverpa armigera (the cotton bollworm), avoided the midvein and periphery of Arabidopsis thaliana rosette leaves and fed almost exclusively on the inner lamina. This fee...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shroff R,Vergara F,Muck A,Svatos A,Gershenzon J

更新日期：2008-04-22 00:00:00

abstract：:Protein-protein interactions are essential for life. Yet, our understanding of the general principles governing binding is not complete. In the present study, we show that the interface between proteins is built in a modular fashion; each module is comprised of a number of closely interacting residues, with few intera...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Reichmann D,Rahat O,Albeck S,Meged R,Dym O,Schreiber G

更新日期：2005-01-04 00:00:00

abstract：:Voltage-gated Na(+) channels initiate action potentials during electrical signaling in excitable cells. Opening and closing of the pore of voltage-gated ion channels are mechanically linked to voltage-driven outward movement of the positively charged S4 transmembrane segment in their voltage sensors. Disulfide locking...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： DeCaen PG,Yarov-Yarovoy V,Scheuer T,Catterall WA

更新日期：2011-11-15 00:00:00

abstract：:Members of the LEF-1/TCF family of transcription factors have been implicated in mediating a nuclear response to Wnt signals by association with beta-catenin. Consistent with this view, mice carrying mutations in either the Wnt3a gene or in both transcription factor genes Lef1 and Tcf1 were previously found to show a ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Galceran J,Hsu SC,Grosschedl R

更新日期：2001-07-17 00:00:00

abstract：:Insulin-like growth factors I and II (IGF-I and -II) are potent mitogens for various cancers, including carcinoma of the prostate. In several experimental cancers, treatment with antagonists of growth hormone-releasing hormone (GH-RH) produces a reduction in IGF-I and -II, concomitant to inhibition of tumor growth. To...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lamharzi N,Schally AV,Koppán M,Groot K

更新日期：1998-07-21 00:00:00

abstract：:To determine the importance of the thrombin substrate recognition exosite for fibrinogen binding in the formation of both arterial and venous thrombi, we evaluated the antithrombotic effects of the tyrosine-sulfated dodecapeptide from residues 53-64 of hirudin (H peptide) in a nonhuman primate model. This peptide was ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cadroy Y,Maraganore JM,Hanson SR,Harker LA

更新日期：1991-02-15 00:00:00

abstract：:The kidney develops through reciprocal interactions between two precursor tissues: the metanephric mesenchyme and the ureteric bud. We previously demonstrated that the zinc finger protein Sall1 is essential for ureteric bud attraction toward the mesenchyme. Here, we show that Kif26b, a kinesin family gene, is a downst...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Uchiyama Y,Sakaguchi M,Terabayashi T,Inenaga T,Inoue S,Kobayashi C,Oshima N,Kiyonari H,Nakagata N,Sato Y,Sekiguchi K,Miki H,Araki E,Fujimura S,Tanaka SS,Nishinakamura R

更新日期：2010-05-18 00:00:00

abstract：:Molecular cloning has demonstrated a diversity of artificially expressed N-methyl-D-aspartate (NMDA) receptors, implying a similar diversity of naturally occurring NMDA receptors in situ. Particularly significant was the success in expression of NMDA receptor classes exhibiting various sensitivities to Mg2+ block, a v...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kato N,Yoshimura H

更新日期：1993-08-01 00:00:00

abstract：:Plants require metals for essential functions ranging from respiration to photosynthesis. These metals also contribute to the nutritional value of plants for both humans and livestock. Additionally, plants have the ability to accumulate nonessential metals such as cadmium and lead, and this ability could be harnessed ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rogers EE,Eide DJ,Guerinot ML

更新日期：2000-10-24 00:00:00

abstract：:Gap junctions are specialized membrane regions that seem to mediate intercellular communication. They appear to contain closely packed arrays of equally sized particles all of which, upon freeze-cleaving, remain attached to one membrane leaflet and not to the other. One gap junction cleavage face, therefore, always ex...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Staehelin LA

更新日期：1972-05-01 00:00:00

abstract：:In most organisms, light plays a key role in the synchronization of the circadian timing system with the environmental day-night cycle. Light pulses that phase-shift the circadian clock also induce the expression of period (per) genes in vertebrates. Here, we report the cloning of a zebrafish per gene, zfper4, which i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vallone D,Gondi SB,Whitmore D,Foulkes NS

更新日期：2004-03-23 00:00:00

abstract：:Frame-shift mutations at microsatellites occur as a time-dependent function of polymerase errors followed by failure of postreplicational mismatch repair. A cell-culture system was developed that allows identification of intermediate mutant cells that carry the mutation on a single DNA strand after the initial DNA pol...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gasche C,Chang CL,Natarajan L,Goel A,Rhees J,Young DJ,Arnold CN,Boland CR

更新日期：2003-02-18 00:00:00

abstract：:In epithelia, a primary damage of tight junctions (TJ) always leads to a secondary disruption of adherens junction (AJ), and vice versa. This response, if occurring in the testis, would disrupt spermatogenesis because the blood-testis barrier (BTB) must remain intact during the transit of spermatids in the seminiferou...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lie PP,Chan AY,Mruk DD,Lee WM,Cheng CY

更新日期：2010-06-22 00:00:00